Evaluation of Millet (Panicum miliaceum subsp. miliaceum) Germplasm For Seed Fatty Acids Using Near-Infrared Reflectance Spectroscopy

이영이,Jung Bong Kim,Ho Sun Lee,Young-A Jeon,Sok Young Lee,Chung Kon Kim 한국작물학회 2012 Korean journal of crop science Vol.57 No.1

The objective of this study was to rapidly evaluate fatty acids in a collection of millet (Panicum miliaceum subsp. miliaceum) of different origins so that this information could be disseminated to breeders to advance germplasm use and breeding. To develop the calibration equations for rapid and nondestructive evaluation of fatty acid content, near-infrared reflectance spectroscopy (NIRs) spectra (1104-2494 nm) of samples ground into flour (n=100) were obtained using a dispersive spectrometer. A modified partial least-squares model was developed to predict each component. For foxtail millet germplasm, our models returned coefficients of determination (R2) of 0.89, 0.89, 0.89, and 0.92 for palmitic acid, oleic acid, linoleic acid, and total fatty acids, respectively. The prediction of the external validation set (n=10) showed significant correlation between references values and NIRs values (r2=0.64, 0.90, 0.79, and 0.89 for palmitic acid, oleic acid, linoleic acid, and total fatty acids, respectively). Standard deviation/standard errors of cross-validation (SD/SECV) values were close to 3 (2.62, 2.40, 1.85, and 2.23 for palmitic acid, oleic acid, linoleic acid, and total fatty acids, respectively). These results indicate that these NIRs equations are functional for the mass screening and rapid quantification of the oleic and total fatty acids characterizing millet germplasm. Among the samples, IT153514 showed an especially high content of fatty acids (48.14 mg g-1), whereas IT123909 had a very low content (34.44 mg g-1).

철학의 장(場)으로서 장르 판타지의 가치 - 이영도의 『드래곤 라자』, 『퓨처 워커』를 중심으로

이영서 대중서사학회 2024 대중서사연구 Vol.30 No.2

본고의 목적은 이영도의 작품을 분석함으로써 장르 판타지의 연구적 가치를 입증하는 것이다. 웹소설 시장의 약진으로 장르문학에 대한 담론이 어느 때보다 활성화되고 있음에도 아직 학계의 관심은 크지 않은 편인데, 이는 보수적인 관점에서 장르 판타지의 특수한 형식·구조와 그것으로부터 창출되는 고유한 연구적 가치가 충분히 규명되지 않았기 때문인 것으로 보인다. 이러한 문제의식에 따라 본고에서는 문학 연구에서 가장 전통적이라고 할 수 있는 서사이론과 철학 담론을 활용하여 ‘철학의 장(場)’으로서 장르 판타지의 가치를 입증하고자 했다. 먼저 『드래곤 라자』의 경우, 가상의 세계관을 통해 종족・지리・도구뿐 아니라 신화・역사・언어 등 새로운 문화를 창조함으로써 장르 판타지에서 배경이 가지는 특수한 위상과 역할을 잘 보여준다. 특히 ‘유피넬-헬카네스의 인식론’은 포스트구조주의의 코스모스-카오스의 존재론과 연결되어 독자들의 철학적 사유를 자극한다. 『퓨처 워커』는 이러한 철학적 배경 속에서 ‘고정된 현재’라는 미증유의 사건을 만들어냄으로써 문제의식을 심화한다. 현재가 고정되면서 나타나는 유령의 현존・도래할 것의 중지와 같은 사건들은 데리다의 시간관을 상징적으로 반영하고 있는데, 이로 말미암아 『퓨처 워커』의 세계는 현실 규범뿐 아니라 서사 규범마저 뛰어넘는 철학적 사고실험장으로 기능한다. 그리고 이러한 철학적 배경과 사건 속에서 수행되는 다양한 인물들의 선택과 갈등은 철학적 사고실험을 서사화 하는 좋은 예시가 된다. 이렇듯 이영도 문학은 장르 판타지로서 현실 규범과 서사 규범 양자로부터 자유롭다는 장점을 십분 활용하여, 흥미롭고도 직관적인 방식으로 독자들에게 근원적인 철학적 질문을 던진다. 바로 이 지점에서 장르 판타지는 순수문학보다도 철학에 대해 더 넓은 개방성을 가지고 있으며, 앞으로는 이러한 가치를 인정받아 장르 판타지와 이영도 문학에 대한 연구가 더욱 활발해질 것으로 기대된다. The purpose of this paper is to prove the literary and research value of the genre of fantasy, by analyzing two of Lee Young-do’s novels. Although discourse on genres like fantasy is more active than ever due to the rapid development of the web-novel market, interest has not yet sparked in academia, because the unique form and structure of genres and its inherent research value have not been sufficiently clarified from a conservative point of view. While taking this issue into consideration, this paper attempts to prove the value of fantasy as ‘a Field of philosophy’ by using narrative theory and philosophical discourse, which are the most traditional in literary study. First of all, in the case of Dragon-Raja, the novel shows the unique status and role of the setting in fantasy which implies the creation of new cultures such as myths, history, and language, as well as race, geography, and tools, in a virtual world. In particular, ‘Eupinel-Helkanes’ epistemology’ is linked to Cosmos-Khaos’ ontology of post-structuralism and stimulates readers’ philosophical thinking. Future-Walker further allows to delve into the issue at hand. In this novel, the author creates an unprecedented event called ‘fixed present’ in this philosophical setting. Events such as the presence of a ghost and the stop of to-come, that appear as the present is symbolically fixed, reflect Derrida’s view on time. As a result, the world of Future-Walker functions as a field of philosophical thought experiment that goes beyond not only reality-code but also narrative-code. In addition, the choices and conflicts of various characters carried out in these philosophical settings and events serve as a good example of how to turn philosophical thought experiments into narrative. As such, Lee Young-do’s works, being categorized as fantasy novels, take full advantage of being free from both reality-code and narrative-code, asking readers fundamental philosophical questions in an interesting and intuitive way. At this point, the genre of fantasy has a wider openness to philosophy than ‘pure literature’, and from now on, this value is expected to be recognized, and research on fantasy novels and Lee Young-do’s works is expected to become more active.

언론사 여성리더 시대

李英伊(이영이) 관훈클럽 2004 관훈저널 Vol.- No.92

게슈탈트 꿈작업

이영이 전국대학교학생생활상담센터협의회 2013 전국대학교학생생활상담센터협의회 연차대회 Vol.- No.7

일하멍 배우멍 - 볍씨학교 제주학사 이야기

이영이 교육공동체벗 2018 오늘의 교육 Vol.- No.47

일(一) 대학병원의 간호수기시 병원 감염괸리 실태조사

이영이,이경희,이영희,임종미 凡石學術奬學財團 1999 凡石學術論文集 Vol.3 No.-

초저온보존 및 인위노화 처리가 인삼 개갑종자의 발아율에 미치는 영향

이영이,이정윤,김장욱,백형진,윤문섭,Kotnala Balaraju 한국약용작물학회 2018 한국약용작물학술대회 발표집 Vol.2018 No.10

Background : Ginseng seeds are one of short-lived seeds species which loose their viability easily in the condition of conventional storage. Cryopreservation using liquid nitrogen (LN) has been recommended as a alternative storage for this kind of germplasm short lived or dessiccation-sensitive. This study was performed to find out whether cryopreservation could assure initial viability not only for seeds with high germination rate but also for seeds with low germination rate (in aging process). Methods and Results : In this work, 3 cultivars of dehisced ginseng seeds were artificial aged in the condition of 40℃, 95% RH during 6 hours with 3 hours-interval. The germination rate of ginseng seeds was decreased to the range of 71 - 94% of initial viability by artificial aging treatment. After 24 hours of vapor-LN exposure on both of artificial aged and non treated seeds as a cryopreservation, germination rate for each cultivar was decreased with the range of 76 - 95% of initial viability. While the decreasing patterns of germination rate for each cultivar showed similar curves between before and after vapor-LN exposure, the aging effects could be slightly little by cryopreservation for 3 cultivars of ginseng seeds. Conclusion : From the above results, we may suggest that cryopreservation could be recommened for storage tool of dehisced ginseng seeds even with low viability also and expected to make slower seed aging process during preservation period through further study.

들깨 종자 활력 수준별 초저온 동결보존 후 발아율 및 Ascorbate Peroxidase 활성 변화

이영이,이명희,이정윤,이태윤,손은호,박홍재 한국환경농학회 2017 한국환경농학회지 Vol.36 No.4

식물 종자를 비롯한 각종 유전자원을 액체질소에 저장하면 유전형질 특성의 손실 없이 장기간 보존할 수 있다. 본 연구에서 보존조건이 까다로워 단명 종자로 분류되는 들깨 종자를초저온 동결저장 방법으로 보존할 수 있는지를 조사하였다. 수집한 들깨 품종별 종자의 초기 발아율은 40-95% 수준으로 다양하였는데, 수분함량을 3-8%로 조절한 종자를 액체질소에 처리하여도 발아율은 감소하지 않았다. 종자의 수분함량이 4-5%인 종자의 초저온 처리 후 발아율이 가장 높았으며, 초기 발아율이 낮은 품종에서는 초저온 처리에 의해 발아율이 증가하기도 했다. 인위 노화처리에 따라 종자의 발아율과ascorbate peroxidase 활성은 감소하였으며, 품종별로 종자의활력 저하 정도는 크게 달리 나타났다. 대조 처리 종자와 비교하였을 때, 초저온 처리과정에 발생할 수 있는 산화스트레스가 들깨 종자의 활력을 저해하지는 않을 것으로 추정되었다. 따라서 들깨 종자를 4-5% 수분함량으로 건조시켜 초저온동결 저장하면 활력 손실 없이 장기간 보존할 수 있을 것으로판단되며, 노화가 급속히 진행되는 품종의 경우에는 고활력자원을 확보하거나 휴면타파 처리 후 초저온 동결 보존하는것이 유리할 것으로 생각된다. BACKGROUND: Seed of perilla (Perilla frutescens var. japonica Hara) is short-lived in conventional storage conditions. For long-term conservation of plant species, cryopreservation is the method currently available. This study was performed to find out reliable methods for a long-term storage of seeds of perilla as a genetic resource. METHODS AND RESULTS: Using seeds of 9 perilla cultivars, the effects of desiccation, aging, andcryopreservation on seed germinability and ascorbate peroxidase activity in the seeds were investigated. Initial germinability of the seeds was various, and dry seeds of all cultivars survived cryopreservation without loss of viability. The highest germination was achieved at 4-5% moisture content, and stimulatory effect of cryogenic temperature on the seed germination was observed in some cultivars. Accelerated aging of perilla seeds led to reduction in germination and ascorbate peroxidase activity, and the susceptibility of seeds to aging was different among the tested cultivars. No significant difference in germination was observed for the aged seeds of control and liquid nitrogen exposed. CONCLUSION: The results of this study suggest that cryopreservation at 4-5% moisture content would be a suitablemethod for long-termconservation of perilla seeds without detrimental effects on germination.

독일 통일 7년 후 : 심리학적 연구대상으로서 구동서독 지역간 불평등

이영이 오늘의 문예비평 1998 오늘의 문예비평 Vol.- No.-

Inhibition of mitotic clonal expansion mediates fisetin-exerted prevention of adipocyte differentiation in 3T3-L1 cells

이영이,배은주 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.11

Adipocytes are the key player in adipose tissueinflammation and subsequent systemic insulin resistanceand its development involves complex process of proliferationand differentiation of preadipocytes. Fistein, apolyphenol flavonoid, is known to exert anti-inflammatory,anti-carcinogenic and anti-diabetic effects. In this study,we aimed to investigate the effect of fisetin on adipocyteproliferation and differentiation in 3T3-L1 preadipocytecell line and its mechanism of action. We found that fisetininhibits adipocyte differentiation in a concentrationdependent manner, which were evidenced by Oil Red Ostaining and the protein expression of mature adipocytemarker genes fatty acid synthase and peroxisome proliferator-activated receptor c. Moreover, the proliferation ofpreadipocytes was also markedly suppressed by treatmentof fisetin for 24 and 48 h in the differentiation medium. Wealso found that fisetin inhibition of adipocyte differentiationwas largely due to the effect on mitotic clonalexpansion. Fisetin suppression of preadipocyte proliferationat early stage of differentiation was accompanied bythe changes of expression of a series of cell cycle regulatoryproteins. Altogether, our results suggest that theinhibition of adipocyte differentiation by fisetin may be atleast in part mediated by cell cycle arrest duringadipogenesis.