http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
RT-PCR에 의한 카네이션괴저바이러스와 카네이션둥근반점바이러스 정밀진단
이시원,강은하,허노열,김상목,김유정,신용길 한국식물병리학회 2013 식물병연구 Vol.19 No.1
카네이션은 세계 3대 절화용 화훼작물의 하나로, 농가생산액 210억 원에 이르는 주요작물이다. 이들은 절화,종자, 묘 및 삽수의 4 품목으로 수출입 되고 있다. 카네이션과 같은 영양번식성 작물의 경우, 증식하는 과정 중에 바이러스의 확산과 전파가 용이한데, 우리나라에서는카네이션괴저바이러스(CNFV)와 카네이션둥근반점바이러스(CRSV)를 식물검역 바이러스로 지정하여 수입검사를수행하고 있다. 본 연구에서는 CNFV와 CRSV를 신속,정밀하고 쉽게 진단할 수 있는 특이적인 프라이머를 고안하였으며, 높은 검출 감도를 가지는 nested 프라이머 조합을 개발하였다. CNFV를 검사하기 위해 최종 선발된특이적인 프라이머는 2 세트로 288과 447 bp를, CRSV를검사하기 위해 최종 선발된 특이적인 프라이머는 2 세트로 503과 549 bp를 증폭하였다. CNFV의 nested는 2 세트 모두 147 bp로 동일하며, CRSV는 각각 395와 347 bp의 밴드를 증폭하였다. 또한, 실험의 신뢰도를 높이기 위하여, 증폭산물에 염기서열 6개를 삽입한 플라스미드를제작하여 양성대조구로 활용하였다. 본 연구에서 개발한방법은, 향후 CNFV와 CRSV에 대한 신속, 정밀한 국경검역을 지원할 수 있을 것이라고 기대된다. Carnation is considered to be one of the top three cutting flowers in the world, which is a main crop with 21billion annual volume of manufacture. The four carnation items such as cuttings, seed, plant and unrooted cuttings are imported and exported. Viruses can be easily transmitted during vegetative propagation of carnation. Carnation necrotic fleck virus (CNFV) and Carnation ringspot virus (CRSV) are designated as Korea plant quarantine viruses and inspected. This study was aimed to develop specific primer sets for easy and rapid detection of CNFV and CRSV. Two RT-PCR primer sets were efficiently amplified 288 and 447 bp fragments for CNFV and 503 549 bp fragments for CRSV. Furthermore, developed nested primer sets make possible to high sensitive detection and verification. CNFV nested PCR primer sets all produced band of 147 bp and CRSV nested PCR primer sets did bands of 395 and 347 bp. In addition, plasmid inserted 6sequences in amplicon were used as a positive control to improve inspection confidence. The successful application of PCR module newly developed in this study will be highly useful for detect of CNFV and CRSV for quarantine inspections.
이시원,최영식,박요한,오경승,신중우,김일진,Shinya Uchino 대한내분비학회 2005 Endocrinology and metabolism Vol.20 No.1
Multiple endocrine neoplasia I(MEN I) is a genetic disorder that consists of neoplasia of neuroendocrine type in the parathyroid glands, in the islets of Langerhans in the pancreas, and in the anterior pituitary gland. Primary hyperparathyroidism is the most common feature and occurs in approximately 95% of MEN I patients. Pancreatic islet cell tumors occur in 40% of MEN I patients. Most of these tumors produce excessive amounts of hormones, such as gastrin, insulin, glucagon and vasoactive intestinal polypeptide(VIP). VIP-producing pancreatic tumors(VIPoma) associated with MEN I are rare and so far only one has been reported in Korea.Recently, we came across a case of MEN I, associated VIPoma presented persistent hypercalcemia after a parathyroidectomy. A 70 year old man had suffered from large amount of watery diarrhea, severe general weakness and paralysis of lower limbs for 3 months which suggests symptoms of hypercalcemia. Before the patient visited our hospital, he underwent subtotal parathyroidectomy due to hyperparathyroidism. Even though he was operation, there was no subsidization of the symptoms and abnormal findings of blood chemistry such as hypercalcemia, hypocalemia were remained unchanged. However, the parathyroid hormone level was still within normal limits. Abdominal computerized tomography scan demonstrated a mass of 2.5cm diameter in tail of the pancreas. As serum level of VIP hormone was also elevated, distal pancreatectomy was carried out to performed. There was improvement in the symptoms towards the normal condition and the level of biochemical parameters such as serum potassium, calcium and VIP, were also within the normal limits. In a direct sequence analysis, GAC→CAT(Asp→His) point mutation, at codon 383 of exon 9 of the MEN I gene was identified in both the patient and his son. The authors report a rare case of VIPoma associated with MEN I with review of literature on MEN I 저자들은 부갑상선 아전절제술 이후에도 교정되지 않는 고칼슘혈증과 계속된 수양성 설사로 인한 하지 마비 및 전신 쇄약을 주소로 내원한 70세 남자 환자에서 일반 화학 검사, 호르몬 검사, 방사선 검사 등으로 VIPoma와 동반된 MEN I을 진단하여 외과적 절제술 후 수양성 설사, 저칼륨혈증 및 고칼슘혈증이 호전되었을 뿐아니라 환자와 환자의 아들에게 시행한 DNA 염기서열 분석에서 MEN I 유전자 exon 9의 codon 383에서 GAC가 CAT(Asp→His)로 전이되는 점돌연변이가 확인된 1예를 경험하였기에 문헌 고찰과 함께 보고하는 바이다.