Monitoring 4-Chlorobiphenyl-Degrading Bacteria in Soil Microcosms by Competitive Quantitative PCR

이수연,MinSupSong,유경만,김배훈,방성호,이인수,김치경,박용근 한국미생물학회 2002 The journal of microbiology Vol.40 No.4

The competitive quantitative PCR method targeting pcbC gene was developed for monitoring 4-chlorobiphenyl( 4CB)-degrading bacteria, Pseudomonas sp. strain DJ-12, in soil microcosms. The method involves extraction of DNA from soil contaminated with 4CB, PCR amplification of a pcbC gene fragment from the introduced strain with a set of strain-specific primers, and quantification of the electrophoresed PCR product by densitometry. To test the adequacy of the method, Pseudomonas sp. strain DJ-12 was introduced into both contaminated and non-contaminated soil microcosms amended with 4CB. Pseudomonas sp. strain DJ-12 was monitored and quantified by a competitive quantitative PCR in comparison with 4CB degradation and the result was compared to those obtained by using the conventional cultivation method. We successfully detected and monitored 4CB-degrading bacteria in each microcosm and found a significant linear relationship between the number of 4CB-degrading bacteria and the capacity for 4CB biodegradation. The results of DNA spiking and cell-spreading experiments suggest that this competitive quantitative PCR method targeting the pcbC gene for monitoring 4CBdegrading bacteria appears to be rapid, sensitive and more suitable than the microbiological approach in estimating the capacity of 4CB biodegradation in environmental samples. 4CB)-degrading bacteria, Pseudomonas sp. strain DJ-12, in soil microcosms. The methodinvolves extraction of DNA from soil contaminated with 4CB, PCR amplification of a pcbC gene fragmentfrom the introduced strain with a set of strain-specific primers, and quantification of the electrophoresedPCR product by densitometry. To test the adequacy of the method, Pseudomonas sp. strainDJ-12 was introduced into both contaminated and non-contaminated soil microcosms amended with4CB. Pseudomonas sp. strain DJ-12 was monitored and quantified by a competitive quantitative PCRin comparison with 4CB degradation and the result was compared to those obtained by using the conventionalcultivation method. We successfully detected and monitored 4CB-degrading bacteria in eachmicrocosm and found a significant linear relationship between the number of 4CB-degrading bacteriaand the capacity for 4CB biodegradation. The results of DNA spiking and cell-spreading experimentssuggest that this competitive quantitative PCR method targeting the pcbC gene for monitoring 4CBdegradingbacteria appears to be rapid, sensitive and more suitable than the microbiological approachin estimating the capacity of 4CB biodegradation in environmental samples.

종로 귀금속 및 보석산업의 직접 특성에 관한 연구

이수연 대한지리학회 2003 대한지리학회 학술대회논문집 Vol.- No.-

취약성 자기애가 조망수용에 미치는 영향: 반성적 반응양식의 매개효과

이수연,이수림 한국교육치료학회 2019 교육치료연구 Vol.11 No.3

This study is to examine the relationships with Vulnerable Narcissism, Perspective-taking and Reflection and to clarify the mediating effect of Reflection between Vulnerable Narcissism on Perspective-taking. The research surveyed 290 people using Vulnerable Narcissism, Perspective-taking, and Reflection scale. The collected data was analyzed by independent sample t-test, correlation analysis, a multiple regression analysis of the mediating effect with SPSS 21.0 program. The results of this study were as following: Firstly, The study shows significant positive correlation between vulnerable narcissism and perspective taking and significant positive correlation between response to reflection. Secondly, The study shows a result of the mediating effect of reflection between vulnerable narcissism on perspective-taking. The response to reflection had a significant effect of complete mediation effect on the relationship between vulnerable narcissism and perspective taking. 본 연구는 취약성 자기애, 반성적 반응양식, 조망수용 간의 관계를 살펴보고, 취약성 자기애가 조망수용에 미치는 영향에서 반성적 반응양식의 매개효과를 확인하고자 하였다. 이를 위해 290명을 대상으로, 취약성 자기애, 조망수용, 반성적 반응양식 척도에 대한 설문조사를 실시하였다. 취약성 자기애에서 조망수용에 이르는 경로를 검증하기 위해 SPSS 21.0을 이용하여 일반적인 기술통계와 상관분석 및 매개효과 검증을 위한 중다회귀분석을 하였다. 연구의 결과를 요약하면 다음과 같다. 첫째, 상관분석 실시 결과, 취약성 자기애는 조망수용과의 관계에서 유의한 정적상관을 보였으며, 반성적 반응양식과도 정적인 상관을 보였다. 둘째, 취약성 자기애가 조망수용에 미치는 영향에서 반성적 반응양식의 완전매개효과가 있는 것으로 나타났다. 이에 본 연구 결과의 함의와 제한점을 논의하였다.

상지로봇재활치료가 만성기 뇌졸중 환자의 상지 및 인지기능, 일상생활활동에 미치는 효과

이수연,장기연,강미영 대한신경치료학회 2022 신경치료 Vol.26 No.2

Purpose The aim of this study was to investigate the effects of robotassisted therapy to improve upper limb and cognitive function and performances of activities of daily living(ADL). Methods We analyzed 6 month followup data of 22 chronic stroke patients including 11 experimental and 11 control patients. Experimental group underwent the robotassisted therapy using ArmeoⓇSpring and general occupational therapy. Control group underwent only general occupational therapy. Outcome measures were used Manual Muscle Test (MMT), Passive Range of Motion (PROM) for upper extremities function. KoreanMini Mental State Examination (KMMSE) and Global Deterioration Scale (GDS) for cognitive function. KoreanModified Barthel Index (KMBI) for ADL performances. Results After intervention, 11 experimental patients significantly increased muscle strength, and showed tendency of improvement PROM and general cognitive function, not statistically. Both of experimental and control group were showed improvement of ADL performances. Conclusion Robotassisted therapy based on virtual reality would be useful and effective intervention to upper limb and cognitive function and performance ability of ADL in chronic stroke patients.

고령자의 근로관계 종료와 정당성 판단기준

이수연 한국노동법학회 2013 노동법학 Vol.0 No.46

하이라이트 : 형체 결합 단백질 기반 면역센서 개발 동향

이수연,장정호,이진형 한국화학공학회 2012 NICE Vol.30 No.1

유화제가 약과의 품질에 미치는 효과

이수연,김명애 한국조리과학회 2002 한국식품조리과학회지 Vol.18 No.3

This study was conducted to examine the effect of emulsifiers on the quality characteristics of Yackwa. Lecithin and various sucrose fatty acid esters (S-570, S-970, S-1570) were used as emulsifiers. The addition of emulsifiers to Yackwa decreased the hardness, cohesiveness, springiness and gumminess, but increased the brittleness. This tendency was most distinct by the addition of S-970. All sensory characteristics of Yackwa such as color, smooth surface, smell, internal layer formation, softness and overall quality, were improved greatly by the addition of emulsifiers. As a result, the mechanical and sensory characteristics of Yackwa were improved by the addition of the emulsifiers, and sucrose fatty acid esters showed better results than lecithin.

사물인터넷(IoT) 환경을 위한 안전한 그룹 키 관리 기법

이수연 한국융합보안학회 2016 융합보안 논문지 Vol.16 No.7

최근 스마트 기기의 보급화로 Wi-Fi, LTE 등을 이용한 무선인터넷 사용률이 대폭 증가한 반면 유선인터넷의 비율은 감소하고 있는 추세이다. 이렇듯 장소에 구애받지 않고 무선인터넷에 접속하여 사람과 사람뿐만이 아니라 사람과 사물간의 통신, 사물과 사물간의 통신인 사물인터넷의 범위가 점점 다양해지고 있는 추세이다. 사물인터 넷(Internet Of Thing)의 빠른 확산과 함께 사물인터넷 보안 위협에 대한 우려도 크게 늘고 있다. 본 논문에서는 사물인터넷 환경에서 그룹 통신을 위한 키 분배와 관리에 대한 새로운 기법을 제안한다. 제안 기법은 키를 분배 하지 않고 그룹에 속한 사물이 자신의 비밀 정보와 공개 값을 가지고 계산한 그룹 키를 사용하여 그룹 멤버 사이 에 안전한 그룹 통신이 가능하게 된다. Recently, the popularity of smart devices such as Wi-Fi and LTE has increased the use ratio of wireless dramatically. On the other hand, the use ratio of wired internet is decreasing. The IoT(Internet of Things) is not only for people but also for communication between people and things, and communication between things and things by connecting to a wireless without choosing a place. Along with the rapid spread of the IoT there is a growing concern about the threat of IoT security. In this paper, the proposed scheme is a efficiency group key agreement in IoT environment that guarantees secure communication among light-weight devices. The proposed scheme securely be able to communication with the group devices who share a group key, generated by own secret value and the public value. Such property is suitable to the environment which are required a local area and a group

함수 호출의 안전성 향상을 돕는 스마트 계약 코드 재작성기

이수연,정형근,조은선 한국정보보호학회 2019 정보보호학회논문지 Vol.29 No.1

When a Solidity smart contract has a problem in calling a function of another contract, the fallback function is supposedto be executed automatically. However, it may be are arbitrarily created, with their behaviors unknown to developers, andfallback function execution is vulnerable to exploits by attackers. in In this paper, we propose a preprocessing based methodto reduce the risk with less overhead of developers’. Developers mark the intention using the newly defined keywords inthis paper, and the preprocessor reduces the risk by preprocessing the conditional variables and conditional statementsaccording to the keywords. Solidity에서 다른 계약의 함수를 호출할 때 특정 문제로 인해 호출할 수 없으면 fallback 함수가 실행된다. 이fallback 함수는 임의로 작성될 수 있는 함수로 어떤 동작을 하는지 정해지지 않았기 때문에 함수의 동작을 알 수없어 공격에 이용되기 쉽다. 본 논문에서는 이러한 위험성을 개발자의 부담 없이 줄이기 위해 전처리기를 이용한 해결방법을 제안한다. 개발자는 새롭게 정의된 키워드를 이용해서 의사표시를 하고, 전처리기는 해당 키워드에 따라상태변수와 조건문을 이용해서 전처리 과정을 진행하여 위험성을 줄인다.

토픽 모델링을 활용한 ‘수돗물 유충’ 뉴스 빅데이터 분석

이수연,김태종 한국콘텐츠학회 2020 한국콘텐츠학회논문지 Vol.20 No.11

본 연구는 ‘수돗물 유충’ 사태의 뉴스 빅데이터를 분석함으로써, ‘수돗물 유충’과 관련된 주요 키워드와 토픽을 파악하여, 환경문제에 대한 위기 대응력 제고방안을 제안하기 위해 수행되었다. 2020년 7월 13일부터 8월 31일까지 보도된 1,975건의 ‘수돗물 유충’ 뉴스를 토픽 모델링 기법으로 분석하였다. 그 결과 언론에서 나타난 ‘수돗물 유충’ 사태가 발생기, 확산기, 수습기로 구분되며, 각 5개의 토픽을 선정하여, 환경문제의 발생과 추진 과정을 확인할 수 있었다. 분석 결과를 바탕으로 환경문제에 대한 위기 대응 방안을 다음과 같이 제언하였다. 첫째, ‘수돗물 유충’ 사건을 중심으로 얽혀있는 다양한 맥락을 탐구하고 통합적인 안목을 형성하는 교육으로 환경문제에 대한 대응력을 기를 수 있도록 해야 한다. 둘째, 인터넷 커뮤니티를 활용한 시민참여의 환경정보 공유와 환경감시 역할 부여가 필요하다. 셋째, 신속하고 정확한 환경정보 제공과 소통을 담당하는 환경 커뮤니케이터의 양성 및 배치가 필요하다. 본 연구는 ‘수돗물 유충’ 관련 뉴스 빅데이터를 기반으로 국내에서 처음으로 토픽 모델링 분석기법을 활용하여 분석한 연구로서, 비정형 데이터로 나타나는 환경 관련 이슈를 실증적이고 체계적으로 분석했다는 학술적 의의와 환경교육 및 커뮤니케이션 개선 방안을 제시했다는 정책적 의의를 지닌다. This study was conducted to propose measures to improve crisis response to environmental issues by analyzing the news big data on the ‘tap water larvae’ situation and identifying related major keywords and topics. To accomplish this, 1,975 cases of 'tap water larvae' reported between July 13 to August 31, 2020 were divided into three periods and analyzed using topical modeling techniques. The analysis output 15 topics for each period. According to the result, the ‘tap water larvae’ incident, as reported in the media, is divided into the occurrence, diffusion, and rectification stages. The government’s response and civilian risk consciousness and reaction could also be seen. Based on the result, the following measures to respond to environment risk is proposed. First, it is necessary to explore the various intertwined context with the ‘tap water larvae’ incident at its core and develop responsiveness to environmental problems through education which forms integrated views. Second, a role to monitor the environment must be implemented and civilian-participated environmental information must be shared through the application of internet communities. Third, the cultivation and deployment of environmental communicators who provide and communicate fast and accurate environment information is required. This study, as the first in Korea to use the topic modeling analysis method based on big data related to 'tap water larvae', has academic significance in that it has empirically and systematically analyzed environmental issues which appear as unstructured data. It also political significance as it suggests ways to improve environmental education and communication.