국내육성 톨 페스큐 품종간 생육특성 및 종자생산성

이상훈,최기준,김기용,지희정,황태영,박형수,김종근,이기원,Lee, Sang-Hoon,Choi, Gi Jun,Kim, Ki-Yong,Ji, Hee Chung,Hwang, Tae Young,Park, Hyung Soo,Kim, Jong Gun,Lee, Ki-Won 한국축산환경학회 2014 한국축산시설환경학회지 Vol.20 No.4

A new tall fescue variety (Festuca arundinacea Schreb.) named Greenmaster and Purumi were. In this study, we evaluated the seed production of new tall fescue domestic varieties were cultivated in Korea. Agronomic aspects of tall fescue domestic varieties were determined using seed production and forage nutritive value of straw at the National Institute of Animal Science from 2013 to 2014. The heading date according to the varieties displayed differences and domestic varieties showed enhanced disease resistance. The harvested seed yields of Greenmaster was 35% higher as 1,664 kg/ha and it's also yield of straw higher than that of Fawn. However, the nutritive value of both varieties was similar. The dry matter yield of Pumumi was 25% higher as 11,080 kg/ha. Accordingly, developed a new variety of domestic tall fescue with excellent environmental adaptability, aiming to make contribution to the vitalization of the Korean grassland industry.

수중충격파를 이용하여 충격고화와 반응합성으로 제조된 Ti₅Si₃ 금속간 화합물의 표면코팅 층의 특성에 관한 연구

이상훈,Lee, Sang-Hoon 한국분말야금학회 2008 한국분말재료학회지 (KPMI) Vol.15 No.2

The objective of the present study is to investigate the increase in the functional characteristics of a substrate by the formation of a thin coating layer. Thin coating layers of $Ti_5Si_3$ have high potential because $Ti_5Si_3$ exhibits high hardness. Shock induced reaction synthesis is an attractive fabrication technique to synthesize uniform coating layer by controlling the shock wave. Ti and Si powders to form $Ti_5Si_3$ using shock induced reaction synthesis, were mixed using high-energy ball mill into small scale. The positive effect of this technique is highly functional coating layer on the substrate due to ultra fine substructure, which improves the bonding strength. These materials are in great demand as heat resisting, structural and corrosion resistant materials. Thin $Ti_5Si_3$ coating layer was successfully recovered and showed high Vickers' hardness (Hv=1183). Characterization studies on microstructure revealed a fairly uniform distribution of powders with good interfacial integrity between the powders and the substrate.

SNS 데이터를 활용한 관광지 혼잡도 및 방문자 특성 분석에 관한 연구

이상훈,김수연,Lee, Sang Hoon,Kim, Su-Yeon 한국산업정보학회 2022 한국산업정보학회논문지 Vol.27 No.5

SNS has become a very close service to our daily life. As marketing is done through SNS, places often called hot places are created, and users are flocking to these places. However, it is often crowded with a large number of people in a short period of time, resulting in a negative experience for both visitors and service providers. In order to improve this problem, it is necessary to recognize the congestion level, but the method to determine the congestion level in a specific area at an individual level is very limited. Therefore, in this study, we tried to propose a system that can identify the congestion level information and the characteristics of visitors to a specific tourist destination by using the data on the SNS. For this purpose, posting data uploaded by users and image analysis were used, and the performance of the proposed system was verified using the Naver DataLab system. As a result of comparative verification by selecting three places by type of tourist destination, the results calculated in this study and the congestion level provided by DataLab were found to be similar. In particular, this study is meaningful in that it provides a degree of congestion based on real data of users that is not dependent on a specific company or service.

우주개발과 진공기술의 응용

이상훈,서희준,유성연,Lee, Sang-Hoon,Seo, Hee-Jun,Yoo, Seong-Yeon 한국진공학회 2008 Applied Science and Convergence Technology Vol.17 No.4

진공이란 공간의 기체압력이 대기압보다 낮은 상태, 즉 분자밀도가 약 $2.5{\times}10^{19}$분자/$cm^3$ 보다 적은 상태를 의미하며, 극청정환경 제공, 단열효과, 입자의 장거리 비행가능, 증발과 승화작용, 안정된 플라즈마를 유지, 생화학 반응 억제, 우주환경 제공 등의 특성으로 인해 오늘날 전 산업분야 및 과학기술 분야에 응용이 되고 있다. 우주환경은 이러한 고진공 환경과 태양 복사열에 의한 고온 환경 및 극저온이 반복되는 가혹한 환경으로 특징지어지는데, 위성체는 지상에서 발사되어 우주궤도에 진입한 순간부터는 계속해서 우주환경에 노출된다. 위성체가 이러한 가혹한 우주환경에 노출될 경우 주요부품에 기능장애가 초래되기도 하며 이는 결국 임무의 실패로 이어지기도 한다. 따라서 위성체는 지상에서 우주환경시험을 거쳐 기능 및 작동상태를 점검해야 하며, 이를 위해서는 우주환경을 모사 할 수 있는 우주환경 모사장비가 필요하다. 우주환경모사장비라 함은 우주환경의 주특징인 고진공상태와 극저온 환경을 모사할 수 있는 지상장비를 말하며, 장비의 설계 및 제작에는 기본적으로 진공기술이 응용되게 된다. 본 논문에서는 한국항공우주연구원이 보유하고 있는 인공위성의 개발에 필수 장비인 우주환경모사장치들을 소개하고, 최근 발달된 국내 진공기술로 국산화 제작에 성공한 유효제원 ${\varphi}8m{\times}L10\;m$ 급의 대형열진공챔버를 통하여 우주개발에 응용되는 진공기술을 소개하고자 한다. Vacuum is any air or gas pressure less than a prevailing pressure in an environmental or, specifically, any pressure lower than the atmospheric pressure and is used by a wide variety of scientists and engineering - including clean environment, thermal insulation, very long mean free path, plasma, space simulation[1]. The space environment is characterized by such a severe condition as high vacuum, and very low and high temperature. Since a satellite will be exposed to such a space environment as soon as it goes into its orbit, space environmental test should be carried out to verify the performance of the satellite on the ground under the space environmental conditions. A general and widely used method to simulate the space environment is using a thermal vacuum chamber which consists of vacuum vessel and thermally controlled shroud. As indicated by name of vacuum chamber, the vacuum technology is applied to design and manufacture of the thermal vacuum chamber. This paper describe the vacuum technology which is applied to space business.

톨 페스큐 신품종 '그린마스터2호'의 품종 특성 및 수량성

이상훈,김기용,지희정,황태영,박형수,채현석,이기원,Lee, Sang-Hoon,Kim, Ki-Yong,Ji, Hee Jung,Hwang, Tae Young,Park, Hyung Soo,Chae, Hyun Seok,Lee, Ki-Won 한국초지조사료학회 2015 한국초지조사료학회지 Vol.35 No.1

본 시험은 엔도파이트 프리 톨 페스큐 신품종을 개발하기 위하여 2008년부터 2014년까지 농촌진흥청 국립축산과 학원 초지사료과에서 수행되었다. 톨 페스큐 신품종 '그린마스터2호'는 6배체 품종으로서 잎은 녹색이며, 출수기가 5월 16일경으로서 대비품종인 'Fawn' 보다 5일 늦은 중생품종이고, 출수기에 초장은 82.5 cm 정도이다. 지역적응시험 4개 지역평균 건물수량은 '그린마스터2호'가 15.1톤/ha로서 대비품종인 'Fawn' 보다 29% 많았으며, 지역별로는 4개 지역 모두에서 'Fawn' 보다 생산성이 우수하였다. '그린마 스터2호'의 사료가치는 조단백질(CP) 함량이 13.9%, 건물소화율(IVDMD)이 69.9%, 가소화양분총량(TDN)이 63.8%, 중성세제불용섬유(NDF)가 59.4%, 산성세제불용섬유(ADF) 는 33.5%로서 Fawn과 대등하였다. '그린마스터2호'는 엔도파이트에 감염되지 않는 품종이면서 'Fawn' 보다 국내 기후환경 적응성과 영속성이 우수하여 초지조성에 알맞은 품종으로서 우리나라 전국에서 재배 가능하다. A new tall fescue variety (Festuca arundinacea Schreb.) named 'Greenmaster2ho' was developed by the National Institute of Animal Science, Rural Development Administration, in Cheonan, Korea from 2010 to 2014. For the synthetic seed production of this new variety, 5 superior clones were selected and polycrossed: 09XFa02, 09XFa03, 09XFa11, 09XFa13, and 09XFa14. The agronomic growth characteristics and forage production capability of the seeds were studied at Cheonan from 2010 onward, and regional trials were conducted in Cheonan, Hoengseong, Jeju, and Jinju from 2012 to 2014. Greenmaster2ho showed resistance to disease, persistence, and regrowth ability that were all enhanced when compared with Fawn. At 15,119 kg/ha, the dry matter (DM) yield of Greenmaster2ho was 29% higher than that of Fawn, but the nutritive value of both varieties as forage was similar. This study aimed to make a contribution to the vitalization of the Korean grassland industry by developing a new tall fescue variety with excellent environmental adaptability.

염과 건조 스트레스 조건에서 톨 페스큐의 종자 발아율과 유전자 발현 변화분석

이상훈,이기원,최기준,김기용,지희정,황태영,이동기,Lee, Sang-Hoon,Lee, Ki-Won,Choi, Gi Jun,Kim, Ki-Yong,Ji, Hee Jung,Hwang, Tae Young,Lee, Dong-Gi 한국초지조사료학회 2014 한국초지조사료학회지 Vol.34 No.2

염 또는 건조 스트레스 처리에 의한 톨 페스큐 종자의 발아율 변화와 유식물체 수준에서의 유전자 발현을 조사하기 위하여 in vitro 조건에서 NaCl과 PEG를 처리하여 분석하였다. NaCl 처리시 톨 페스큐 품종별 발아율은 50 mM 농도에서 발아율이 서서히 감소하기 시작하였으며 350 mM의 농도에서는 모든 품종에서 발아가 되지 않는 경향을 보였다. NaCl 처리 농도에 따른 발아율 감소율은 Fawn 품종이 가장 큰 변화를 보였으며 Kentucky-31(E-) 품종이 가장 강한 내성을 보였다. 또한, PEG 처리시 톨 페스큐 품종별 발아율의 변화도 NaCl 처리시와 유사한 경향을 보였으며 고농도인 30% PEG 처리구에서는 모든 품종에서 발아가 되지 않는 경향을 보였으며 Kentucky-31(E-) 품종이 가장 강한 내성을 보였다. 톨 페스큐 유식물체 수준에서 염해와 건조 스트레스에 의한 유전자 발현양상을 조사하기 위하여 DEGs (differentially expressed genes) 탐색을 위한 ACP-based GeneFishing$^{TM}$ PCR 분석을 통해 NaCl 또는 PEG 처리에 따른 발현량의 차이를 보이는 총 4개의 DEG를 선발하여 클로닝하고 염기서열을 분석하였다. 무처리구에 비해 NaCl 처리시 4개의 DEG가 증가하였고 감소하는 DEG는 확인 되지 않았으나, PEG 처리에서는 3개의 DEG (DEG 1, 3, 및 4)가 증가하였고 1개의 DEG가 감소하는 경향을 나타내었다. 발굴된 DEG들을 blastx 검색에 의하여 rubisco large subunit (DEG1), microsomal glutathion S-transferase (GST) 3-like isoform 1 (DEG2) 유전자로 동정되었다. Salinity and drought stresses are probably the most significant abiotic factor limiting plant's growth, also negatively affect seed germination and early seedling development. To study on effect of NaCl and PEG stress on seed germination and gene expression pattern of tall fescue, the levels of NaCl and PEG-induced water stresses were determined in first experiment. Different concentration of NaCl (0 to 350 mM) and PEG (0 to 30%) were used for seed treatment. Seed Germination percentage reduced with increasing osmotic potential of growth medium either due to NaCl or PEG. Seeds were not germinate at 350 mM NaCl or 30% PEG treatment. On the basis of the results, Kentucky31(E-) had more resistant than Fawn in both stress conditions. Furthermore, we have used an annealing control primer-based differential display reverse transcription-polymerase chain reaction method to identify salt- and drought stress-induced differentially expressed genes (DEGs) in tall fescue leaves. Using 120 annealing control primers, a total of 4 genes were identified and sequenced. The possible roles of the identified DEGs are discussed in the context of their putative role during salinity and drought stresses.

봉독약침이 류마티스 관절염 환자의 기능회복 및 삶의 질에 미치는 영향

이상훈,이현종,박상민,김수영,박재경,홍승재,양형인,최도영,김건식,이두익,이윤호,이재동,Lee, Sang-Hoon,Lee, Hyun-Jong,Park, Sang-Min,Kim, Su-Young,Park, Jae-Kyung,Hong, Seung-Jae,Yang, Hyung-In,Choi, Do-Young,Kim, Keon-Sik,Lee, Doo-Ik,Lee, 대한약침학회 2002 Journal of pharmacopuncture Vol.5 No.2

Objective: To evaluate the effects of bee venom acupuncture(BVA) on the rehabilitation and quality of life in rheumatoid arthritis(RA) patients Methods: Patients with RA were treated with the BVA therapy twice a week for 3 months. Tender joint counts, swollen joint counts, morning stiffness, Erythrocyte Sedimentation Rate(ESR), C-reactive protein(CRP), patient global assessment, physician global assessment, Korean health assessment questionnaire(KHAQ) were estimated and analyzed before and after BVA therapy. Results: Tender joint counts, swollen joint counts, morning stiffness showed significant decrease after BVA therapy. But, as acute inflammatory reactants, ESR showed no significant difference and CRP showed significant increase after BVA therapy. Patient global assessment, physician global assessment, and KHAQ index showed significant improvement after BVA therapy. Conclusions: BVA therapy can improve rehabilitation and health-related quality of life in RA patients as well as clinical symptoms and signs. Further study is required in more population with large scale including acute inflammatory reaction of BVA therapy.

연부 조직에 발생한 악성 횡문근양 종양 - 증례 보고 -

이상훈,김한수,오주한,서성욱,이한구,Lee, Sang-Hoon,Kim, Han-Soo,Oh, Joo-Han,Suh, Sung-Wook,Lee, Han-Koo 대한근골격종양학회 2000 대한골관절종양학회지 Vol.6 No.4

횡문근양 종양은 주로 소아기의 신장에서 발생하는 신생물로, 침습적인 양상을 보이는 비교적 드문 종양이다. 최근에 신장 외의 다양한 부위에서 발생한 예들이 보고되고 있으며, 연부 조직에 발생한 경우에는 미분화된 횡문근 육종으로 오진되기 쉽다. 횡문근양 종양은 횡문근 육종보다 침습적이고 나쁜 예후를 보이는 종양으로, 횡문근 육종과의 조직학적인 정확한 감별이 필요하며, 면역조직화학 검사가 그 감별에 도움을 줄 수 있다. 저자들은 치골 부위와 하복벽 부위에서 발생한 횡문근양 종양을 경험하여 문헌의 고찰과 함께 보고하고자 한다. Malignant rhabdoid tumor is a highly aggressive tumor of children, that often arises in the kidney. Some rhabdoid tumors have been reported in various extra-renal location including the central nervous system, liver, skin, and soft tissues. In case of arising in soft tissues, it may be misdiagnosed as rhabdomyosarcoma. It is important to distinguish malignant rabdoid tumor from rhadomyosarcoma, because malignant rhabdoid tumor has more aggressive behavior and poorer survival rate. And this differential diagnosis can be performed by several immunohistochemistry. Here we report a case of malignant rhabdoid tumor that arose in lower abdominal wall with related articles.

국내 방사선치료기기의 품질관리 현황조사 및 분석

이상훈,김주리,조삼주,조광환,임천일,김혁주,허현도,신동오,권수일,최진호,Lee, Sang-Hoon,Kim, Ju-Ree,Cho, Sam-Ju,Cho, Kwang-Hwan,Lim, Chun-Il,Kim, Hyeog-Ju,Huh, Hyun-Do,Shin, Dong-Oh,Kwon, Soo-Il,Choi, Jin-Ho 한국의학물리학회 2010 의학물리 Vol.21 No.2

국내 개설된 방사선종양학과 중 선형가속기 및 근접치료기기가 설치되어 환자치료를 시행하고 있는 총 72개 기관에 대해 설문을 수행하였다. 설문은 선형가속기, 근접치료기기 등의 품질관리 효율성을 파악하기 위해 주기별 품질관리 수행여부, 수행인력 및 주체, 수행 시간을 조사하였다. 선형가속기 및 근접치료기기에 대한 각각의 품질관리 검사 항목에 대한 주기별 수행 여부를 조사하였다. 조사결과 품질관리 검사항목 수에서 미국 및 유럽의 품질관리 지침서와 비교하여 일일 및 주간점검 항목수의 경우는 8~10로서 유사한 결과를 보였으나, 월간과 연간점검 항목 수는 미국 및 유럽의 경우 17~21 항목이었고, 국내의 경우는 15.5로 낮게 나타났다. 또한 국내의 여건은 품질관리를 수행 할 인력 및 시간의 부족과 제한으로 인해 미국 및 유럽의 품질관리 지침서에서 권고하는 소요인력 및 시간과 비교하여 대략 50% 수준의 낮은 수치를 보였다. 따라서 본 연구에서 수집한 국내 방사선종양학과의 품질관리 검사항목 및 실행 주기, 수행 인력 및 수행시간에 관한 현황 조사 결과와 기존에 발표된 미국의학물리학회 TG-40 보고서 및 유럽의 품질관리 지침서를 활용한다면 국내 실정 및 각 기관에 적합한 품질관리 지침서를 수립하는데 유용할 것으로 기대된다. We have taken surveys about total 72 departments of radiation oncology which is performing the treatment with linear accelerator and brachytherapy unit in Korea. The survey was included the research about the linear accelerator, brachytherapy, Also, we surveyed the various performance (QA period, manpower, time) of quality control for understanding of efficiency. The survey results show that the QA test of daily and weekly are almost same comparing to USA and Europe but the QA performance of monthly and yearly in Korea are 15.5 which is less than USA and Europe recommended QA item number of 17 to 21. The manpower and QA time in Korea also lower than 50% of USA and Europe recommended because the manpower and QA time limitation in Korea. It will be expected that the manual of quality management in each clinic could be appropriately established when combining the present results with previously published AAPM TG-40 and other protocols.

재발성 거대 세포종의 치료

이상훈,김한수,장종범,이한구,Lee, Sang-Hoon,Kim, Han-Soo,Chang, Chong-Bum,Lee, Han-Koo 대한근골격종양학회 1999 대한골관절종양학회지 Vol.5 No.1

Between Sep. 1986 and Mar. 1996, twenty four patients with recurrent giant cell tumor of their long bones were treated and followed up for more than two years. We applied three different modalities for treatment: curettage with bone graft, curettage and packing with polymethylmethacrylate, wide excision with or without reconstruction. During the average 51.5 months after operation(24-97 months), two of three patients who underwent curettage with bone graft showed recurrence. Patients who underwent wide excision showed no recurrence. Five of fifteen patients who underwent curettage and packing with polymethylmethacrylate showed recurrence. Four of seven who showed recurrence were treated with reapplication of curettage and packing with polymethylmethacrylate. All four patients showed no recurrence, and two of them have been followed up for more than two years. We suggest that the curettage and packing with polymethylmethacrylate is an effective treatment modality of the recurrent giant cell tumor.