인간포배기 배아의 효과적인 유리화 동결법의 개발을 위한 연구

이상민,이주희,이상원,이승민,윤산현,임진호,박흠대,이성구,Lee, Sang-Min,Lee, Ju-Hee,Lee, Sang-Won,Lee, Seoung-Min,Yoon, San-Hyun,Lim, Jin-Ho,Park, Huem-Dai,Lee, Seong-Goo 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.3

Objective: The purpose of this study was to evaluate the survival rate of vitrified blastocyst according to the freezing vessels, equilibration time in cryoprotectant and artificial dehydration method. Methods: Human blastocysts were vitrified after loading onto the plastic straw, open-pulled straw (OPS), electron microscopy grid (EM grid) for 1.5 min or 3 min. They also were directly plunged into LN2 within 30sec. For artificial shrinkage of blastocysts, 36 gauge fine needle was pushed at the cellular junction of the trophectoderm into the blstocoele cavity until it shrank without damage of inner cell mass. Results: The survival rate of vitrified blastocysts on plastic straw, OPS, EM grid as freezing vessels were 26.7, 13.0 and 60.5%, respectively. The survival rate of EM grid was significantly higher than that of plastic straw and OPS (p<0.05). For 1.5 min equilibrium, the survival rates of early blastocyst (EB), middle blastocyst (MB) and late blastocyst (LB) were 64.4, 81.0, and 20.0% respectively. For 3 min equilibrium, the survival rates of EB, MB, and LB were 69.9, 50.0 and 57.5% respectively. The survival rates of EB and MB were significantly higher than that of LB in 1.5 min equilibrium group (p<0.05), however, the significance was not observed in 3 min equilibrium groups. In cytoplasmic shrinkage before vitrification, the survival rates of EB, MB and LB were 92.9, 100 and 75.9% respectively. The survival rate of MB was significantly higher than that of LB (p<0.05). The survival rates of vitrified blastocysts by artificial dehydration and slow-frozen blastocysts were not significantly different as 88.9 and 66.7%, respectively. Conclusion: This study showed that the vitrification of human blastocysts using EM grid and artificial dehydration is an effective method. Therefore, these methods would be an useful techniques for blastocyst cryopreservation.

학교시설 통합정보시스템 구축방안에 관한 연구 - 정보화 구축설계 방향을 중심으로 -

이상민,노근철,유승호,Lee, Sang Min,Ro, Keun Chul,Yoo, Seung Ho 한국교육녹색환경연구원 2017 교육·녹색환경연구 Vol.16 No.1

학교 시설이 가장 중요한 공공시설 중 하나라는 사실에도 불구하고 학교시설에 대한 정보가 체계적으로 관리되지 않고 있는 실정이다. 이에 본 연구에서는 학교시설 정책의 가장 기본적인 요소인 학교시설 현황에 대한 객관적인 데이터를 체계적으로 확보하기 위한 시스템 구축방향에 대한 연구를 진행하였다. 본 연구에서는 학교 시설 정보 관리 절차 및 학교시설의 정보 자료 관리 수준을 향상시킬 수있는 시스템 구축방향을 제안하였으며, 실무적인 활용이 가능한 서비스 제공을 위한 시스템 구축 설계방향을 제시하였다. Despite the fact that school facilities are one of the most important public facilities, information about school facilities is not systematically managed. The purpose of this study was to investigate the direction of system construction to provide objective data on school facilities, which is the basic element of school facility policy. In this study, we propose a system construction method to improve the information management procedure of school facility information and school facilities, and suggest a system design direction for providing service that can be practically used.

경두개 자기 자극이 인지 기능에 미치는 영향

이상민,채정호,Lee, Sang Min,Chae, Jeong-Ho 대한생물정신의학회 2016 생물정신의학 Vol.23 No.3

Transcranial magnetic stimulation (TMS) is a safe, noninvasive and useful technique for exploring brain function. Especially, for the study of cognition, the technique can modulate a cognitive performance if the targeted area is engaged, because TMS has an effect on cortical network. The effect of TMS can vary depending on the frequency, intensity, and timing of stimulation. In this paper, we review the studies with TMS targeting various regions for evaluation of cognitive function. Cognitive functions, such as attention, working memory, semantic decision, discrimination and social cognition can be improved or deteriorated according to TMS stimulation protocols. Furthermore, potential therapeutic applications of TMS, including therapy in a variety of illness and research into cortical localization, are discussed.

"연구실안전환경조성법"의 배경과 주요 내용

이상민,Lee, Sang-Min 한국화재보험협회 2007 防災와 保險 Vol.120 No.-

지난 2005년 3월에 재정되 "연구실안전환경조성법"은 과학기술관련 연구실에 대한 특화된 안전과리체계의 수립과 더불어 산업재해로 인정받지 못해 충분한 보상을 받을 수 없었던 연구실 사고피해에 대한 보상내용을 담고 있다.

녹색건축 전문가 자격제도 개발 연구

이상민,이승민,Lee, Sang Min,Lee, Seung Min 한국교육녹색환경연구원 2014 교육·녹색환경연구 Vol.13 No.1

In an effort to resolve environmental pollution problems, developed countries enforce green building certification systems. The same is true in Korea. However compared professional accreditation system to certification systems did not systematically in Korea. Therefore, in accordance with development of green building related systems and technology the requirement for foster green building professionals is urgent. Accordingly this study is purposed to analyze and propose that green building professional accreditation system development and operation of the direction. Through data analysis domestic and international green building professional accreditation system for draw a development plan. And then conduct an expert Delphi survey reflected in the survey results to development plan.

동의보감(東醫寶鑑) 중(中) 목통(木通)이 주약(主藥)으로 배오(配伍)된 처방(處方)의 활용(活用)에 대한 고찰(考察)

이상민,권택현,옥인수,윤용갑,Lee, Sang-Min,Kwon, Taek-Hyun,Ok, In-Soo,Yun, Young-Gab 대한한의학방제학회 2005 大韓韓醫學方劑學會誌 Vol.13 No.1

This report describes the remedial fields, symptoms, pathology, dosage, prescriptional constitution of 46 studies related to the use of Lignum akebiae main blended prescriptions from Dongeubogam. The following conclusions were reached through investigations on the prescriptions that use Lignum akebiae as a key ingredient. Lignum akebiae blended prescriptions are utilized 17.4% of prescriptions appear in the chapter of urine. Prescriptions that utilize Lignum akebiae as the main ingredient are used commonly in the treatment of the symptoms of a urine and a cystic disease related to the urinary organs, or the diseases of eye, ear, nose, throat and the symptoms of a tumor, abscess. Lignum akebiae is utilized for an etiological and pathologenic fators related to heat, dampness, phlegm and summer heat such as excessive fever of heart and bladder, fever of small intestine, lower energizer dampness and heat, fever due to blood deficiency, phlegm fire, affection by summer heat and dampness etc. The clinical dosage of Lignum akebiae is 1 don(about 3.75gram), according to symptoms, it has ranged at a wide variety of amounts from 3 pun(about 1.13gram) to 1.5 don(about 11.25gram). Lignum akebiae is widely applied with base prescriptions such as Dozuksan, Moktongchajuntang, Manjunmoktongtang. etc.

추출방식에 따른 방풍통성산(防風通聖散)이 지방세포 대사에 미치는 영향

이상민,김병우,임태진,김동희,권기록,Lee, Sang-Min,Kim, Byoung-Woo,Rhim, Tae-Jin,Kim, Dong-Heui,Kwon, Ki-Rok 대한약침학회 2008 Journal of pharmacopuncture Vol.11 No.1

Objective : The purpose of this study is to investigate the effects of Bangpungtongsung-san extracts on the preadipocytes proliferation, of 3T3-L1 cell line. lipolysis of adipocytes in rat's epididymis and localized fat accumulation of porcine by extraction methods(alcohol and water). Methods : Diminish 3T3-L1 proliferation and lipogenesis do primary role to reduce obesity. So, 3T3-L1 preadipocyte and adipocytes were performed on cell cultures, and using Sprague-Dawley rats for the lipogenesis, and treated with 0.01-$1mg/m{\ell}$ Bangpungtongsung-san Extracts depend on concentrations. Porcine skin including fat tissue after treated Bangpungtongsung-san Extracts by means of the dosage dependent variation are investigated the histologic changes after injection of these extracts. Results : Following results were obtained from the 3T3-L1 preadipocyte proliferation and lipolysis of adipocyte in rats and histologic investigation of fat tissue. 1. Bangpungtongsung-san extracts were showed the effect of decreased preadipocyte proliferation on the high dosage($1.0mg/m{\ell}$). 2. Bangpungtongsung-san extracts were showed the effect of decreased the activity of glycerol-3-phosphate dehydrogenase(GPDH) on the high dosage($1.0mg/m{\ell}$) and Specially, alcohol extract of Bangpungtongsung-san was clear as time goes by high concentration. 3. Bangpungtongsung-san extracts were showed tries to compare the effect of lipolysis, alcohol extract of Bangpungtongsung-san on the high dosage($1.0mg/m{\ell}$) was observed the effect is higher than water extract. 4. Investigated the histological changes in porcine fat tissue after treated Bangpungtongsung-san extracts, we knew that water extract of Bangpungtongsung-san was showed the effect of lipolysis on the high dosage($10.0mg/m{\ell}$) and alcohol extract of Bangpungtongsung-san was showed significant activity to the lysis of cell membranes in all concentration. Conclusion : These results suggest that Bangpungtongsung-san efficiently induces diminish proliferation of preadipocyte and lipolysis in adipose tissue.

영흥도에서 분리된 Phaeodactylum tricornutum의 증식 및 Monounsaturated fatty acid 관련 지방산 조성 분석

이상민,조용희,신동우,전효남,류영진,임상민,이철균,Lee, SangMin,Cho, Yonghee,Shin, ong-Woo,Jeon, Hyonam,Ryu, YoungJin,Lim, Sang-Min,Lee, Choul-Gyun 한국해양바이오학회 2014 한국해양바이오학회지 Vol.6 No.2

Oxidation stability and cold fuid property are considered as the most important factors for determining biodiesel quality. Among the fatty acids, monounsaturated fatty acid satisfy both oxidation stability and cold flow property of biodiesel quality standards. Microalgae with high monounsaturated fatty acid contents is have some benefit for producing to produce biodiesels with satisfying quality standards. In this study, monounsaturated fatty acid contents of a isolated microalga from Youngheung island was analyzed. Phaeodactylum tricornutum was isolated by streaking, and growth rate and fatty acid composition of the algae were investigated. Total FAME contents were consisted of 26% of saturated fatty acids, 43% of monounsaturated fatty acids, and 18% of polyunsaturated fatty acids. The contents of monounsaturated fatty acid were especially high in the Phaeodactylum species. This result implies that the FAMEs from P. tricornutum may contribute to improve the oxidation stability and cold flow property of biodiesel.

저항 용접을 이용한 금속 샌드위치 판재 접합에 관한 연구 : Part 2 - 공정해석

이상민,김진범,나석주,Lee Sang-Min,Kim Jin-Beom,Na Suck-Joo 대한용접접합학회 2005 대한용접·접합학회지 Vol.23 No.6

In part 1. optimal process parameters such as thickness of stopper and welding time are achieved to produce high strength ISB(Inner Structured and Bonded) panels. Developed process is different from the usual resistance welding process in the number of points welded at a time. In part 2, Numerical modeling for this new process is proposed and the variation of contact area with respect to the gap of electrodes is studied through FE analyses, Besides, it is tried to figure out the welding nugget formation and proper distance between welding points. FE analytic results show that inner structures are melted more than skin plate, and current distribution between points to be welded can be controlled by distance welding points. Comparison of some FE analytic results with corresponding experimental results could confirm the validity of the proposed numerical modeling.

지역특성을 고려한 학교규모 적정화 방향 설정에 관한 연구

이상민,조창희,Lee, Sang-Min,Cho, Chang-Hee 한국교육녹색환경연구원 2016 교육·녹색환경연구 Vol.15 No.1

본 연구는 적정규모학교의 효율적인 육성을 위하여, 적정규모학교에 대한 이론적 고찰, 국내 학생수 및 학교현황 분석, 적정규모학교 육성사업 현황분석, 적정규모학교 가이드라인(안) 제안 등의 순서로 연구를 진행하였다. 적정규모학교 기준(안)은 시 도별 특성과 지역의 여건을 고려하며, 기존 선행연구들의 교육적 효과와 교육과정의 운영 효율성을 고려하여 이상적인 방향으로 제안하고자 한다. 본 연구에서는 적정규모학교의 효율적인 육성을 위하여, 적정규모학교에 대한 이론적 고찰, 국내 학생수 및 학교현황 분석, 적정규모학교 육성사업 현황분석을 통해 적정규모학교 가이드라인(안)을 제안하였다. School appropriate scale refers to the size of the school to minimize students' educational deficits and ensure an appropriate level of students, the number of classes, class size, etc., and maximize educational effects. Difficult set of absolute standards appropriate scale school, contemporary social situations and educational effects, and seek a comprehensive and reasonable direction, considering this is required. School appropriate scale it is preferable to consider the effect of conditions in areas such as education, curriculum, but it is common to set the range in terms of school operational effectiveness, and efficiency of education funding, school environment. So the concept of the appropriate scale including the school's status changes associated with school size, is seeking a new direction is needed so that the school environment, etc. are considered.