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배양포유동물세포에서 환경돌연변이원에 의해 유발된 자매염색분체교환의 분석에 관한 연구
이석우,남정구,남재환,이병광,김은영,이승길 ( Sok Woo Lee,Joung Koo Nam,Jae Hwan Nam,Byoung Kwang Lee,Eun Young Kim,Seung Gil Lee ) 한국환경생물학회 1991 환경생물 : 환경생물학회지 Vol.9 No.2
The assay of SCEs has been used to measure mutagenicity induced several mutagens to high vertebrates. In this study, we used BHK-21, CHO-K1 and human peripheral lymphocyte as culture cell, and calcium chloride dihydrate(CaCl_2·2H_2O), sodium hypochloride(NaOCl), phenol(C_6H_5OH), benzo(a)pyrene(C_20H_12), carbon tetrachloride(CCl_4) and chloroform(CHCl_3) as mutagens. We examined and compared frequency between spontaneous and induced SCEs by mutagens. The mean spontaneous SCEs of human lymphocyte from 4 donors is scored 6.92±1.54(SCEs/cell) and BHK-21 is scored 0.18±0.03(SCEs/chromosome) and CHO-K1 is scored 0.61±0.08(SCEs/chromosome). In order to decided concentration of mutagens which is enough to induce SCEs and to effect SCEs score, we observed cell cytotoxicity and cell metaphase dependent on several concentration of mutagens. And then, using that concentration as control, we examined SCEs frequency induced three different concentration of mutagen. The increase of SCEs frequency induced by calcium chloride dihydrate is very little, but benzo(a)pyrene and phenol are great increase. Especially, we cannot find metaphase over 0.006mM benzo(a)pyrene and 1mM phenol. Although the increase of SCEs frequency induced by CHCl_3 is little, we observed many acentromeric chromosome in cell culture over 25mM CHCl_3. And we detected that SCEs frequency related with chromosome length of test cell.