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닭 뉴캣슬병의 발병기전 규명을 위한 RT-PCR 및 면역조직화학적 연구
이민권 ( Min Kwon Lee ),진영배 ( Young Bae Jin ),문운경 ( Oun Kyong Moon ),김순복 ( Soon Bok Kim ) 한국가축위생학회 2004 韓國家畜衛生學會誌 Vol.27 No.1
The present experiment was carried out to study the pathogenesis of Newcastle disease(ND), ND virus(NDV) antigens and genes in various organs from NDV inoculated chickens were detected by immunohistochemistry and RT-PCR. Immunohistochemically, NDV antigens were detected in the spleen, thymus, cecal tonsil, proventriculus, trachea and lungs at 12 hour post-inoculation (hpi). Viral antigens were localized mainly in the cytoplasm of lymphocytes and macrophages. After 48 hpi, clinical findings of the affected chickens were open-mouth breathing, conjunctivitis, watery diarrhea and edema around the eye and neck. After 72 hpi, chickens showed muscular tremor, paralysis of the legs and wings, and coma. Histopathological results consist of multi-focal necrosis with hemorrhages in lymphoid aggregates of the intestinal tracts, necrosis of the lymphoid tissues, neuronal degeneration and necrosis, and perivascular cuffing. Using RT-PCR, virus genes were detected in the spleen and proventriculus at 48 hpi, and in the brain at 60 hpi.
중간상과의 거래특성이 제조업체의 온라인 경로 활용에 미치는 영향
이민권(Min Kwon Lee),임영균(Young Kyun Lim) 한국유통학회 2004 流通硏究 Vol.9 No.1
본 연구는 국내 제조업체의 온라인 경로 활용이 중간상과의 거래특성에 의해 어떠한 영향을 받는가를 실증분석하고 있다. 국내 81개 제조업체를 대상으로 한 서베이 분석결과, 거래특성변수는 온라인과 오프라인에서 취급되는 제품의 범위에 유의적인 영향을 미치지 않는 것으로 나타났다. 경로기능별 활용도에 있어서는 거래특성변수 중 제조업체의 중간상에 대한 의존도와 제조업체가 인식하는 중간상의 기회주의가 제조업체의 온라인 활용도에 영향을 미치는 것으로 나타났다. 대체로 이들 거래특성변수는 배달, 반품환불, A/S와 같이 전통적으로 오프라인 중간상이 수행하는 것이 보다 경제적으로 효율적인 기능에 있어 상대적으로 더 큰 영향을 미치는 것으로 나타났다. 그러나 제품정보제공 기능의 활용도는 거래특성변수와 무관한 것으로 나타났다. 본 연구는 이상의 분석결과를 토대로 제조업체의 온라인 경로 활용과 관련된 이론 및 실제적 시사점을 제시하고 있다. This study investigates the effects of relationship characteristics on the adoption of online distribution channels in Korea. A questionnaire survey of 81 domestic manufacturing companies revealed that relationship characteristics have no effects on the range of products sold through online channels. In terms of functional usage, manufactures' dependence and their perceptions of middlemen's opportunism were found to affect the extent of adoption of online channels. Overall, these two variables were significantly related to such functions as delivery, refunding, and A/S that have been traditionally considered efficient when implemented by the middlemen. None of the relationship characteristics was found significantly related to the adoption of online channels as a tool of information provision. The authors discuss the theoretical and practical implications of the findings.
경남북부지역의 소 Neospora caninum 및 Toxoplasma gondii 항체 양성률 조사
이민권 ( Min Kwon Lee ),박종식 ( Jong Sik Park ),김민희 ( Min Hee Kim ),박동엽 ( Dong Yeop Park ),김철호 ( Cheol Ho Kim ),김국헌 ( Guk Heon Kim ),조재현 ( Jae Hyeon Cho ) 한국가축위생학회 2011 韓國家畜衛生學會誌 Vol.34 No.3
In the present study, we carried out a seropositive survey of Neospora (N) caninum and Toxoplasma (T) gondii in cattle at northern area of Gyeongnam province, Korea. Serum sample was obtained from Holstein cattle (n=248, 44 farms in 5 counties) and Korean indigenous cattle (n=120, 23 farms in 4 counties). All 368 sera were tested for antibodies to N. caninum and T. gondii by an enzyme-linked immunosorbent assay (ELISA). Seropositive of N. caninum at farm and heads were 41.8% (28/67) and 12.8% (47/368), respectively. Twenty-five cattle (6.8%) were seropositive to T. gondii, and eighteen (26.9%) of 67 studied farms had cows seropositive to T. gondii. The seroprevalence of Holstein cattle was much higher than that of Korean indigenous cattle. There was no apparent association of T. gondii seropositivity with age, while the seropositivity for N. caninum was associated with cattle age. The farm of cattle above the age of 5 years (16.8%) was more infective than less than 4 years (9.3%). This is the first report on seroprevalence of N. canium and T. gondii in Holstein and Korean indigenous cattle in Gyeongnam, Korea.
이민권(Lee Min Kwon),이해성(Lee Hae Sung) 대한토목학회 2006 대한토목학회논문집 A Vol.26 No.1A
이 논문에서는 캔틸레버 공법으로 시공되는 대칭형 강 사장교의 폐합해석법을 제안한다. 폐합시공 전에는 폐합단면 양쪽에서 두개의 독립적인 구조계가 성립되기 때문에 폐합단면에 적절한 단면력을 가해 주어야만 폐합단면에서 수직 처짐과 회전각에 대한 적합조건을 만족시킬 수 있다. 그러나 실제 시공에서는 폐합단면에 단면력을 재하하는 것이 거의 불가능하기 때문에 시공 중에 실제로 교량에 재하할 수 있는 외력에 의하여 적합조건을 만족시켜야 한다. 이 논문에서는 몇 개 케이블의 무응력 길이와 데릭 크레인의 인양력을 조정하여 적합조건을 만족시킬 수 있는 해석 방법을 제안한다. 적합조건식은 케이블의 무응력 길이와 크레인 인양력에 대하여 비선형이므로 Newton-Raphson 방법에 의하여 반복적으로 푼다. 케이블 부재는 탄성현수선 요소를 사용하여 모델링하였고, 주탑과 거더는 일반적인 3차원 뼈대요소를 사용하여 이산화하였다. 교량의 케이블 무응력 길이와 인양력에 대한 변위 민감도는 평형방정식을 직접 미분하여 계산하였다. 주어진 신뢰도 구간에서 시공 오차를 예측하기 위한 몬테-카를로 모사법을 제안하였다. 제안된 방법을 제 2 진도대교의 폐합시공에 적용하여 그 타당성과 효율성을 검증하였다. This paper presents the closing analysis of a symmetric steel cable-stayed bridge erected by a free cantilever method. Two independent structural systems are formed before the closing procedure of a bridge is performed, and thus the compatibility conditions for vertical displacement and rotational angle are not satisfied at the closing section without the application of proper sectional forces. Since, however, it is usually impossible to apply sectional forces at the closing section, the compatibility conditions should be satisfied by proper external forces that can be actually applicable to a bridge. Unstrained lengths of selected cables and the pull-up force of a derrick crane are adjusted to satisfy nonlinear compatibility conditions, which are solved iteratively by the Newton-Raphson method. Cable members are modeled by the elastic catenary cable elements, and towers and main girders are discretized by linear 3-D frame elements. The sensitivities of displacement with respect to the unstrained lengths of selected cables and the pull-up force of the derrick crane are evaluated by the direct differentiation of the equilibrium equation. A Monte-Carlo simulation approach is proposed to estimate expected construction errors for a given confidence level. The proposed method is applied to the second Jindo Grand Bridge to demonstrate its validity and effectiveness.
김유오(Kim Yoo-oh),이민권(Lee Min-kweon) 한국유통과학회 2005 유통과학연구 Vol.3 No.2
In the business environment which enclosed the domestic small and d medium distribution industry, and a business sign of recovery, reduction of income, job insecurity, and a national pension an aging society - positive exhibition of the large-sized store in comparison with the low personal consumption depended uneasy and deregulation, and consumer needs progress of individualization diversification it is ultimately faced with a difficult situation. Therefore the seen research examine the various range of a circulation policy through precedence research. It is going to consider and see the influence which the circulation regulation means of the purpose of large-sized store regulation and a major power is compared, and regulation.
배양세포에서 전염성조혈장기괴사증 바이러스항원의 면역조직화학적 검출
문운경 ( Oun Kyung Moon ),이민권 ( Min Kwon Lee ),진영배 ( Young Bae Jin ),김순복 ( Soon Bok Kim ) 한국가축위생학회 2002 韓國家畜衛生學會誌 Vol.25 No.3
This experiment was done to set up the immunohistochemical detection method for infectious hematopoietic necrosis virus(IHNV) antigens in the monolayers of CHSE-214 cell cultures inoculated with IHNV. Specific identification of IHNV antigens was detected in the cytoplasms of infected cells by the use of monoclonal antibodies to glycoproteins. The specific positive signal was observed as a distinct red color. The result showed that streptavidin alkaline phosphatase immunohistochemistry specifically identified IHNV antigens in infected cultured cells.