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정소희,이미겸,김태일 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.1
Cationic methylcellulose/zoledronic acid nano-complex was developed for efficient gene delivery and anticancer effect. Zoledronic acid (Zol) is typically used for osteoporosis treatments and is known to induce apoptosis in breast cancer cells. Methylcellulose derivative (MCPEI) was synthesized by oxidation of methylcellulose and reductive amination with polyethylenimine. Zol was loaded into MCPEI to form MCPEI/(Zol:pDNA) nano-complex. MCPEI/(Zol:pDNA) nano-complex showed higher transfection efficiency than MCPEI in HeLa cells, due to endosome buffering of imidazole group of Zol. Bafilomycin A1-treated transfection showed significantly decreased efficiency, indicating that transfection is processed by endocytosis. MCPEI/(Zol:pJDK-apoptin) complex showed significant anticancer activity in MDA-MB-231 cells, due to the combinatorial effect of Zol and apoptin. It was concluded that MCPEI/(Zol:pDNA) complex improved the transfection efficiency and showed potential for anticancer treatment.
Synthesis and Characterization of Cationic Hydroxyethylcellulose for Gene Delivery System
박지원,정소희,이미겸,김태일 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.2
Hydroxyethyl cellulose (HEC), one of the water-soluble cellulose ether is widely used for variety of applications including pharmaceutical productions. In this study, conjugate of low molecular weight polyethyleneimine (PEI, Mw 2000) and HEC was developed for gene delivery system. The cationic hydroxyethylcellulose derivative (HECP2k) was synthesized by reductive amination of PEI to periodate-oxidized HEC. It could form the positively charged nano-particles with pDNA. HECP2k-treated cells showed high viability in both serum-free condition and serum condition due to the biocompatibility of HEC and low cytotoxicity of PEI2k. Transfection efficiency of HECP2k polyplex was high, similar with that of PEI25k in both serum-free and serum condition. Therefore, HECP2k showed a potential for gene delivery system.