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윤희준(Hee-Jun Yun),김태윤(Tai-Yun Kim) 한국정보과학회 1993 한국정보과학회 학술발표논문집 Vol.20 No.1
EDI 변환 처리 시스템에서 EDI 표준 데이타베이스의 역할은 크다. 기존의 테이블 방식의 데이타베이스의 구성을 객체지향 기법을 사용한 새로운 접근 방식의 표준 데이타베이스의 구성을 제안한다. EDI 메세지의 구성 요소들의 정의와 속성 및 연산 그리고 구성 요소들간의 관계에 대한 설계를 제안하였다.
반복핵이식에 의한 복제동물 생산에 관한 연구 III. 토끼에서 제3세대 복제수정란의 생산
이효종,전병균,윤희준,박충생,최상용,윤창현,강대진,Lee Hyo-jong,Jeon Byeong-gyun,Yin Xi-jun,Park Choong-saeng,Choe Sang-yong,Yun Chang-hyun,Kang Dae-jin 한국임상수의학회 1995 한국임상수의학회지 Vol.12 No.1
The recycling nuclear transplantation(NT) technique has the powerful potential of producing a large number of genetically identical embryos and offsprings from one embryo. Multiple generational cloning by this technique utilizes the NT embryo itself as the donor for the next generation of cloning. In this experiment, we have produced the third generational cloned embryos by recycling NT. Further we examined comparatively the electrofusion rate and in vitro developmental potential in the cloned embryos of the first second and third generations. The embryos of 16-cell stage were collected from the mated does by flushing oviducts with Dulberco's phosphate buffered saline containing 10 % fetal calf serum(FCS) at 47 hours after hCG injection. In the first generation NT, the nuclear donor embryos were synchronized in the phase of Gl/S transition of 32-cell stage. The first and second generation NT embryos developed to 16-cell were used as donor nuclei for second and third generation. The recipient cytoplasms were utilized the oocytes collected at 14 hours after hCG injection, following revoming the nucleus and the first polar body by micromanipulation. The separated blastomeres were injected into the enucleated recipient oocytes by micromanipulation and were fused by electrical stimulation. The electrofusion rate was seen to be 78.0, 88.0 and 90.3 % in the first second and third generation NT rabbit embryos, respectively. The fused oocytes were co-cultured with a monolayer of rabbit oviductal epithelial cells in M-199 solution containing 10 % FCS for 120 hours at 39$^{\circ}C$ in a 5% $CO_2$ incubator. The in vitro developmental potential to blastocyst stage was significantly(P<0.05) decreased in the third(7.2 %) generation NT embryos compared to the first(53.1 %) and second(16.1 %) generation NT embryos. Following in vitro development to blastocyst stage, they were stained with Hoechst 33342 dye for counting the number of blastomeres by fluorescence microscopy. The mean blastomere numbers and cell cycle numbers of NT embryos during the culture period were significantly(p<0.05) decreased in the second(93.9 cells and 6.55 cylces) and third(81.5 cells and 1.35 cylces) generation, compared to the first(189.9 cells and 7.55 cylces) generation.
컴포넌트를 이용한 XML기반 생물자원정보관리시스템 설계
이계준,손강렬,양진호,윤희준,Lee, Gye-Jun,Son, Gang-Ryeol,Yang, Jin-Ho,Yun, Hui-Jun 한국과학기술정보연구원 2002 지식정보인프라 Vol.10 No.-
생물다양성정보는 크게 생물종다양성 정보 구축과 종정보를 대상으로 구축되어지는 컨텐트정보로 나눠 데이터베이스화한다. 이때, 분야별 데이터베이스 통합은 종정보에 대한 횡적인 통합과 컨텐트에 대한 종적인 통합이 동시에 이루어지며, 분류학자들에 의해 정의된 항목과 국제적인 GSD(Global Species Database) 구축의 표준이 되는 내용들을 기반으로 구성요소들의 표준화 정의와 XML기반 표준 DTD를 작성한다.