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Arbitrary, Complex Cell Patterning via Inkjet Printing of a Cell Membrane-Anchoring Polymer
윤화인,조용우,최지숙,이병국,오제훈 한국고분자학회 2012 Macromolecular Research Vol.20 No.5
Well-defined cell patterns, including both adherent and non-adherent cells, were created using piezoelectric inkjet printing of a cell membrane-anchoring polymer composed of a hydrophobic oleyl group, a hydrophilic poly(ethylene glycol) (PEG) chain, and an amino-reactive N-hydroxy-succinimide (NHS) end group. Various complex living cell patterns were created by surface engineering in which inkjet-printed, membrane-anchoring polymer patterns controlled the geometric distributions of immobilized cells. Non-adherent (SNU-620) and adherent cells (HeLa) were stably anchored and patterned on a glass substrate through interactions between cell membranes and the hydrophobic oleyl groups. The effects of an apoptosis inducer (staurosporine) and an anticancer drug (paclitaxel)were visualized on cells immobilized on the polymer-printed patterns. This approach may have broad utility in most advanced biomedical devices requiring miniaturized and precisely controlled living cell patterns.
백종열, 윤화도 배재대학교 자연과학연구소 2010 自然科學論文集 Vol.21 No.1
This study was carried out to develop new rootstock variety for oriental melon using 4 commercial pumpkin varieties, 7 cucurbita varieties, and 28 germplasms of pumpkin. The Hanaro, Dong-A and Sponge are resistant to Meloidogyne incognita, but showed very low root growing. In pumpkins, CMA16, CMA34 and CMO40 are almost resistant but they also showed bad root growing.