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ACE-Inhibitory Properties of Proteolytic Hydrolysates from Giant Jellyfish Nemopilema nomurai
윤호동,김연계,임치원,윤소미,이문희,문호성,윤나영,박희연,이두석 한국수산과학회 2011 Fisheries and Aquatic Sciences Vol.14 No.3
This study aimed to determine the degree of hydrolysis and angiotensin-I-converting enzyme (ACE)-inhibitory activity of Giant Jellyfish Nemopilema nomurai (jellyfish) hydrolysates. The degree of hydrolysis using six proteolytic enzymes (Alcalase, Flavozyme,Neutrase, papain, Protamex, and trypsin) ranged from 13.1-36.8% and the inhibitory activities from 20.46-79.58%. Using papain hydrolysate, we newly isolated and characterized ACE-inhibitory peptides with a molecular weight of 3,000-5,000 Da that originated from jellyfish collagen. The purified peptide (FII-b) was predicted to be produced from an alpha-2 fragment of the type IV collagen of jellyfish. The N-terminal sequence of FII-b was Asp-Pro-Gly-Leu-Glu-Gly-Ala-His-Gly- and showed 87% identity to the collagen type IV alpha-2 fragment of Rattus norvegicus and a predicted protein from Nematostella vectensis, indicating that the ACE-inhibitory peptide originated from the collagen hydrolysate and had an IC_(50) value of 3.8 μg/mL. The primary structure of the fragment is now being studied; this peptide represents an interesting new type of ACE inhibitor and will provide knowledge of the potential applications of jellyfish components as therapies for hypertension.
윤호동,정은지,최지웅,이민섭,박명애,윤나영,김연계,조득문,김재일,김형락 한국수산과학회 2011 Fisheries and Aquatic Sciences Vol.14 No.4
Bacterial lipopolysaccharide (LPS) induces expression of pro-inflammatory cytokines and enzymes producing nitric oxide (NO) and prostaglandins (PGs) in immune cells. This process is mediated by the activation of nuclear factor kappaB (NF-κB). In this study, we investigated the anti-inflammatory characteristics of Codium fragile ethanolic extract (CFE) mediated by the regulation of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) using LPS-stimulated murine macrophage RAW 264.7 cells. CFE significantly inhibited LPS-induced NO and PGE2 production in a dose-dependent manner and suppressed the expression of iNOS and COX-2 proteins in LPS-stimulated RAW 264.7 cells with no cytotoxicity. Pro-inflammatory cytokines, such as interleukin (IL)-1β, IL-6, and tumor necrosis factor-α, were significantly reduced by treatment of CFE in LPS-stimulated RAW 264.7 cells. CFE inhibited the promoter activity of (NF)-κB in LPS-stimulated macrophages. Treatment with CFE suppressed translocation of the NF-κB p65 subunit by preventing proteolytic degradation of inhibitor of κB-α. These results indicate that the CFE-mediated inhibition of NO and PGE_2 production in LPS-stimulated RAW 264.7 cells is mediated through the NF-κB-dependent transcriptional downregulation of iNOS and COX-2, suggesting the potential of CFE as a nutraceutical with anti-inflammatory activity.
수산물 가공부산물의 이용에 관한 연구 1 - 가다랭이 내장 발효 silage 제조를 위한 유산균주의 배양특성
윤호동(Ho Dong Yoon),이두석(Doo Seog Lee),지청일(Cheong Il Ji),서상복(Sang Bok Suh) 한국수산과학회 1997 한국수산과학회지 Vol.30 No.1
In order to utilize fish by-products from the skipjack tuna (Katsuwonus pelamis) canning manufactures, Lactobacillus bulgaricus KCTC 3188 and L. plantarum KCTC 1048 were used as a starter culture for the preparation of fermented fish silage with skipjack tuna viscera. The optimum temperature and pH on bacterial growth and lactic acid production of L. bulgaricus and L. plantarum in MRS broth were 35℃ and around pH 6.0, respectively. And the optimum concentrations of the carbohydrate sources added to the broths were 7% far dextrose and 10% for molasses on the basis of total weights of skipjack tuna viscera. The pH of acid treated skipjack tuna viscera silage (ASS) slightly increased from 4.0 to 4.5, while that of fermented skipjack tuna viscera silages by the use of lactic acid bacterias (FSS) was significantly declined from 5.9 to about 4.0 after 42 days of storage at 35℃. Though the content of volatile basic nitrogen (VBN) in ASS was lower than those of FSS after 42 days of storage at 35℃, VBN content in silages slightly increased from an initial value of 62∼65 ㎎/100 g to final value of 113∼155 ㎎/100 g over 42 days. The fermented silage by L. plantarum reached a maximum concentration of amino nitrogen and showed 81% of hydrolysis degree after 4 days of storage at 35℃.
수산물 가공부산물의 이용에 관한 연구 2 - 가공방법에 따른 가디랭이 내장 silage 의 저장 중 성분변화
윤호동(Ho Dong Yoon),이두석(Doo Seog Lee),서상복(Sang Bok Suh) 한국수산과학회 1997 한국수산과학회지 Vol.30 No.1
For an effective use of fish by-products from the skipjack tuna (Katsuwonus pelamis) canning manufactures, the changes of chemical properties of skipjack tuna viscera silage by the processing method during storage were investigated. The acid treated skipjack tuna viscera silage (ASS) were higher in the contents of moisture, lipid, protein and mineral but lower in the contents of carbohydrate and polyunsaturated fatty acids than those of fermented skipjack tuna viscera silage (FSS) by L. bulgaricus, KCTC 3188 and L. plantarum, KCTC 1048. Especially, the contents of total n-3 fatty acids in FSS increased remarkably during storage. The dominant amino acids in ASS and FSS were glutamic acid (Glu), aspartic acid (Asp), leucine (Leu), glycine (Gly), and alanine (Ala). And the contents of tryptophan (Trp) decreased by 30% in ASS and 5% in FSS in comparision with that of raw skipjack tuna viscera after 42 days of storage. The concentration of vitamin B₁and B₂in FSS increased gradually during storage but the concentration of vitamin B₂in ASS decreased. In the organoleptic evaluation, ASS gave a grayish brown color and a fishy odor. On the other hand, FSS had reddish brown color and sour taste by the production of lactic acid during storage.
Graphite Nanosheets/PVDF 복합체의 열전 성질
윤호동(Ho Dong Yoon),남승웅(Seung Woong Nam),응우옌두(Nguyen D K Tu),김대흠(Dae Heum Kim),김희숙(Hee Suk Kim) 한국고분자학회 2013 폴리머 Vol.37 No.5
유연 열전소자를 제조하기 위한 열전재료로서, graphite nanosheet(GNS)와 poly(vinylidene fluoride) (PVDF)를 복합화하여 GNS/PVDF 복합체를 제조하였다. GNS의 함량에 따른 전기전도도, 열전도도, 지벡상수를 측정하여열전성능을 확인하였다. GNS의 함량이 10에서 70 wt%로 증가하면서 전기전도도는 389에서 1512 S/m로 향상되는결과를 보였다. 복합체의 전기전도도가 크게 증가하는 반면에 지벡 상수는 26.7에서 31.2 μV/K로 큰 변화를 보이지않았으며, 열전도도 역시 0.24 W/m·K를 유지하면서 변화를 보이지 않았다. 고분자와의 복합화를 통하여 GNS 자체의 높은 열전도도를 낮춤으로써 향상된 열전성능을 갖는 열전재료를 제조할 수 있었다. GNS/PVDF composites were prepared using graphite nanosheets (GNS) and poly(vinylidene fluoride) (PVDF)for flexible thermoelectric application. We measured the electrical conductivity, thermal conductivity and Seebeck coefficient of GNS/PVDF composites with different contents of GNS and then evaluated the thermoelectric properties of GNS/PVDF composites. The electrical conductivity of GNS/PVDF composites increased from 389 to 1512 S/m with increasing the content of GNS from 10 to 70 wt%. While the electrical conductivity dramatically increased, Seebeck coefficient and thermal conductivity did not show any big difference as the content of GNS increases. In this study, we demonstrated that GNS/PVDF composites improved the thermoelectric properties by decreasing the thermal conductivity due to the phonon scattering at the interfaces between polymer and GNS nanoplatelets.