아동문학과 문학교육에 대하여

윤혜명 서울대학교 국어교육과 1971 先淸語文 Vol.2 No.1

한국 재래종 콩 유전자원의 이소플라본 함량과 수량관련형질에 대한 연차간 비교

윤혜명(Hyemyeong Yoon),이정윤(Jungyoon Yi),케베데 타예 데스타(Kebede taye Desta),신명재(Myong-Jae Shin),이윤정(Yoonjung Lee),이수경(Sukyeung Lee),왕샤오한(Xiaohan Wang),최유미(Yu-Mi Choi),이성우(Sungwoo Lee) 한국육종학회 2021 한국육종학회지 Vol.53 No.4

In this study, composition and agronomic traits, including yield-related traits, were investigated and compared among 35 Korean landracegermplasm for two years (2019 and 2020). The contents of 12 isoflavones were measured using high-performance liquid chromatography, and agronomictrait data were collected according to the Rural Development Administration criteria. Comparing isoflavone composition between the two years,all isoflavones, except glycitein, were generally higher in 2019. The values of yield-related and major agronomic traits decreased in most accessionsin 2020. ANOVA showed highly significant differences (p<0.001) in genotypes, years, and genotype-by-year interaction for all isoflavones, the numberof pods per plant, and 100-seed weight. The number of seeds per pod, days to flowering, and days to maturity also showed significant differencesamong genotypes, as well as between the two years. Correlation analysis revealed that daidzin and genistin were positively correlated with theirmalonylated form. Malonyl daidzin was also positively correlated with malonyl genistin levels. The accessions with the least variation in the totalisoflavone content were IT 153844 (907.5 μg/g, 898.6 μg/g) and IT 252252 (663.2 μg/g, 636.4 μg/g). Some of the evaluated landraces will bea promising genetic source for developing soybean varieties with high levels of environmental stability in isoflavone content and agronomic traits.

주요영양성분과 농업적 특성 분석을 통한 한중일 귀리(Avena sativa L.) 유전자원의 원산지별 다양성 비교

이수경,윤혜명,이명철,오세종,무함마드 라우프,허온숙,노나영,이정윤,현도윤,조규택,고호철,최유미 한국육종학회 2019 한국육종학회지 Vol.51 No.1

Oats are known for their abundance of quality proteins and lipids compared to those of other grain crops; they especially contain a large amount of good quality fibers, including β-glucan. The Korean domestic consumption of oats is rapidly increasing due to their high nutritional value. However, the research for functional food breeding material has been insufficient because oats have been focused on as feed. Therefore, this study aimed to evaluate the agronomic traits and important nutritional ingredients, including β-glucan, protein, lipid, fiber, and dietary fiber, of 142 oat germplasms from Korea, China, and Japan, which are maintained in the National Agrobiodiversity Center. In addition, the diversity by origin of the germplasms was analyzed based on their agronomic traits. For the agronomic characteristics, Korean oat germplasms were on average headed and matured earlier than the Chinese and Japanese ones. Seven accessions, including IT151107, were matured before mid-June, which makes double cropping possible in the Southern region of Korea. In the diversity analysis by origin based on quantitative agronomic traits, Korean oat germplasms were in the distinguished group compared to Chinese and Japanese germplasms. Oat germplasm had a wider range of main ingredients compared to that of Korean domestic oat cultivars. However, Chinese germplasms had a significantly higher β-glucan content, while Korean germplasms had significantly higher crude protein, crude lipid, and crude fiber contents, than did other germplasms from other origins. Dietary fiber contents showed no significant differences between origins. IT129802 (China), IT166594 (Korea), IT166584 (Korea), IT151108 (Korea), and IT129798 (China) showed the highest β-glucan, protein, lipid, fiber, and dietary fiber contents, respectively. These selected oat germplasms can be superior breeding materials for various functional and agronomic purposes.

근적외선분광분석에 의한 동아시아 지역 재래종 벼 유전자원의 아밀로스 및 단백질 함량 변이분석

오세종,최유미,윤혜명,이수경,유은애,이명철,Muhammad Rauf,채병수 한국작물학회 2019 한국작물학회지 Vol.64 No.2

본 연구는 선행연구에서 개발된 근적외선 분광분석(NIRS) 예측모델을 활용하여 측정된 국내외 재래종 메벼 유전자원의 아밀로스 및 단백질 함량 자료를 통계처리 하여 자원의지리적 특성과 성분 함량에 대한 정확한 정보를 제공하기위해 실시하였다. 1. 정규분포분석 결과 메벼 유전자원의 아밀로스 평균은22.0%였고, 단백질 평균은 8.2%였으며 전체 자원의 95% 를 차지하는 자원들의 함량범위는 아밀로스의 경우 15.0- 28.9%, 단백질은 5.4-10.9%였다. 자원의 다양성지수는아밀로스의 경우 0.81, 단백질은 0.50이었다. 2. ANOVA, DMRT에 사용된 자원 수는 한국 자원의 경우1,032, 북한은 994, 일본은 800, 중국은 528자원이었다. 국가별 아밀로스 평균함량은 중국 자원의 경우 23.34%, 한국 자원은 21.55%, 일본 자원은 21.45%, 북한 자원은20.48%였다. 단백질 평균함량은 중국 자원의 경우 9.02%, 일본 자원은 8.06%, 북한 자원은 8.04%, 한국 자원은7.99%였다. ANOVA 결과 벼 유전자원의 아밀로스 및단백질 함량은 국가별 차이가 있었고 1% 유의수준에서차이가 인정되었다. 3. DMRT 결과 국가별 아밀로스 함량은 한국과 일본, 북한, 중국의 세 집단으로 나눌 수 있었으며 각 집단 간 아밀로스 함량차이는 1% 유의수준에서 차이가 인정되었다. 단백질 함량의 경우 한국, 일본, 북한과 중국의 두 집단으로 나눌 수 있었으며 각 집단 간 단백질 함량차이는1% 유의수준에서 차이가 인정되었다. 북한 자원은 가장낮은 아밀로스 평균함량을 나타냈고, 한국 자원은 가장낮은 단백질 평균함량을 나타냈다. 이에 비해 중국 자원은 가장 높은 아밀로스와 단백질 평균함량을 나타냈다. 이러한 지리적 분포에 따른 벼 자원 간 함량차이는 각지역별 자원 선호도와 품종 특성이 반영된 결과라고 할수 있다. A statistical analysis of 4,380 non-glutinous landrace rice germplasm collected from four East Asian countries namely South Korea (1,032), North Korea (994), Japan (800), and China (528) was conducted using normal distribution, variability index value (VIV), analysis of variation (ANOVA), and Duncan’s multiple range test (DMRT) based on a data obtained from Near-Infrared Reflectance Spectroscopy (NIRS) analysis. In normal distribution, the average protein content was 8.2%, and the non-glutinous rice amylose, ranging over 10%, was found to be 22.0%. Protein content in most gremplasm was between 5.4 and 10.9%, and amylose content was between 15.0 and 28.9%. The VIV was 0.50 for protein, and 0.81 for non-glutinous rice amylose content. The average amylose content was 23.34% in Chinese, 21.55% in South Korean, 21.45% in Japanese, and 20.48% in North Korean resources, while the average protein content was found to be 9.02% in Chinese, 8.06% in Japanese, 8.04% in North Korean, and 7.99% in South Korean resources. ANOVA of amylose and protein content showed significant differences at p=0.01. The F-test value for amylose content was 94.92, and for protein content was 81.82 compared to the critical value of 3.79. DMRT of amylose and protein content revealed significant differences (p<0.01). Among the various germplasm obtained from different countries, that from North Korean had the lowest level of amylose content, whereas that from South Korea had the lowest level of protein content than all other resources. Chinese resources had the highest level of amylose and protein content. It is recommended to use these results in breeding fields.

근적외선분광분석에 의한 육성계통 벼 유전자원의 아밀로스 및 단백질 성분함량에 관한 통계분석

오세종,최유미,윤혜명,이명철,신명재,유은애,현도윤,채병수 한국육종학회 2019 한국육종학회지 Vol.51 No.4

A statistical analysis for 9,771 non-glutinous rice in breeding line germplasm collected from Korea (2,836), China (2,136), Japan (1,219), and Philippines (1,213) was conducted using normal distribution, variability index value (VIV), analysis of variation (ANOVA) and Ducan’s multiple range test (DMRT) based on the data obtained from NIRS analysis. In normal distribution, the average protein content was 7.9%, and non-glutinous rice ranging over 10% amylose had 23.6% of average content. Most resources were in between 5.3 and 10.5% in protein content, and 15.7 and 31.5% in amylose content. The VIV was 0.54 for protein, and 0.83 for non-glutinous rice amylose content. The average amylose content was 25.18% in Filipino, 24.54% in Chinese, 22.08% in Korean, and 21.47% in Japanese resources, while the average protein content was found to be 8.19% in Filipino, 7.79% in Chinese, 7.58% in Korean, and 7.42% in Japanese resources. The ANOVA of amylose and protein content showed significant differences at the level of 0.01. The F-test value was 412.2 for amylose content, and 108.4 for protein compared to critical value 3.78. The DMRT of amylose and protein content showed significant differences (p<0.01) among resources of other countries. The Filipino resources had the highest level of amylose and protein content, whereas, the lowest level of amylose and protein content were found in Japanese compared to other origins. It is recommended these results be helpful materials to breeding fields.

밀 유전자원의 근적외선분광분석 예측모델에 의한 단백질 함량 변이분석

오세종,최유미,윤혜명,이수경,유은애,현도윤,신명재,이명철,채병수 한국작물학회 2019 한국작물학회지 Vol.64 No.4

A near-infrared reflectance spectroscopy (NIRS) prediction model was set to establish a rapid analysis system ofwheat germplasm and provide statistical information on the characteristics of protein contents. The variability index value (VIV) ofcalibration resources was 0.80, the average protein content was 13.2%, and the content range was from 7.0% to 13.2%. Aftermeasuring the near-infrared spectra of calibration resources, the NIRS prediction model was developed through a regression analysisbetween protein content and spectra data, and then optimized by excluding outliers. The standard error of calibration, R2, and theslope of the optimized model were 0.132, 0.997, and 1.000 respectively, and those of external validation results were 0.994, 0.191,and 1.013, respectively. Based on these results, a developed NIRS model could be applied to the rapid analysis of protein in wheat. The distribution of NIRS protein content of 6,794 resources were analyzed using a normal distribution analysis. The VIV was 0.79,the average protein was 12.1%, and the content range of resources accounting for 42.1% and 68% of the total accessions were 10-13%and 9.5-14.6%, respectively. The composition of total resources was classified into breeding line (3,128), landrace (2,705), andvariety (961). The VIV in breeding line was 0.80, the protein average was 11.8%, and the contents of 68% of total resources rangedfrom 9.2% to 14.5%. The VIV in landrace was 0.76, the protein average was 12.1%, and the content range of resources of 68% of totalaccessions was 9.8-14.4%. The VIV in variety was 0.80, the protein average was 12.8%, and the accessions representing 68% of totalresources ranged from 10.2% to 15.4%. These results should be helpful to the related experts of wheat breeding. 본 연구는 근적외선 분광분석기(NIRS) 예측모델을 설정하여 유전자원 대량분석 체계를 확립하고 그에 따른 국내외 밀 자원의 단백질 함량에 관한 기초 정보를 제공하고자하였다. 1. 농업유전자원센터에 보유하고 있는 20,000여 자원 중1,798자원을 검량 자원으로 선발하였다. 검량자원의 NIR스펙트럼을 측정하였고, 단백질 함량 습식분석 데이터입력 등 일련의 통계적 처리 과정을 거쳐 NIRS 예측모델을 설정했다. 검량 자원의 다양성 지수는 0.80이었고,습식 분석법에 의한 단백질 평균은 13.2%, 함량 구간은7.0-20.8%였다. 최적화된 NIRS 모델의 R2, SEC, Slope은 0.997, 0.132, 1.000이었다. 300자원을 사용하여 외부검정 과정을 실시하였고 R2, SEP, Slope은 0.994, 0.191,1.013이었다. 최적화된 NIRS 모델과 외부검정 결과의통계치가 상호 유사하였고, 1에 가까운 R2와 Slope 값,낮은 SEC와 SEP 값을 볼 때 본 연구에서 설정한 NIRS모델은 습식 분석법을 대체하여 밀 자원의 단백질 함량분석에 적용 가능할 것으로 판단되었다. 2. 국내외 수집된 밀 6,794자원의 NIRS 단백질 함량 측정값을 정규분포로 작성하여 특성을 파악했다. 자원의 다양성 지수는 0.79, 단백질 평균은 12.1%, 전체 자원의임의구간 42.1% 단백질 함량자원 범위는 10-13%이었으며, 68.0%를 차지하는 자원들의 단백질 함량 범위는9.5-14.7%였다. 3. 전체 6,794자원의 품종 집단 구성은 육성계통 3,128자원, 재래종 2,705자원, 육성품종 961자원이었다. 육성계통 자원의 다양성 지수는 0.80, 단백질 평균은 11.8%,전체 자원의 68%를 차지하는 자원들의 함량 범위는 9.2-14.5%였다. 재래종 자원의 다양성 지수는 0.76, 단백질평균은 12.1%, 전체 자원의 68.0%를 차지하는 자원들의 함량 범위는 9.8-14.4%였다. 육성품종 자원의 다양성지수는 0.80, 단백질 평균은 12.8%, 전체 자원의 68.0%를 차지하는 자원들의 함량 범위는 10.2-15.4%였다. 재래종 자원은 가장 낮은 다양성 지수를 나타냈고, 육성계통과 육성품종은 동일한 다양성 지수를 나타냈다. 육성계통은 가장 낮은 단백질 평균을 나타냈고, 육성품종은가장 높은 단백질 평균을 나타냈다.

Evaluation of Major Dietary Ingredients in Diverse Oats (Avena sativa L.) Germplasm

Muhammad Rauf,윤혜명,이수경,신명재,고호철,이명철,오세종,현도윤,허온숙,최유미 한국작물학회 2019 Journal of crop science and biotechnology Vol.22 No.5

Oat (Avena sativa L.) is one of the world’s most important cereal crops, owing to its use as an important source of essential nutrients for both humans and animals. In the present study, the main nutritional components of 975 oat germplasm accessions were investigated. Crude fiber content ranged from 0.08 to 6.79%, with a mean of 2.14%, whereas total dietary fiber content ranged from 5.32 to 17.59%, with a mean of 11.01%, β-glucan content ranged from 1.02 to 6.33%, with a mean of 3.05%, and lipid content ranged from 1.41 to 8.72%, with a mean of 4.73%. Furthermore, most of the germplasm accessions exhibited 1-3% crude fiber content in the range of 1-3%, 9-12% total dietary fiber content, 2-4% β-glucan content, and 4-5% lipid content. Both the crude fiber and total dietary fiber contents were significantly but weakly correlated with β -glucan content, and the nutritional contents of germplasm accessions from different countries varied significantly. The highest mean crude fiber, total dietary fiber, β-glucan, and lipid contents were exhibited by the germplasm accessions collected from Canada, the UK, the Netherlands, and Turkey, respectively, and the accessions that exhibited the highest crude fiber (n=4), total dietary fiber (n=7), β-glucan content (n=5), and lipid (n=12) contents were identified. These findings provide valuable information for breeding programs aiming to improve the nutritional value of already existing high-yielding oat cultivars.

Evaluation of sprout growth traits and flavonoid content in common and tartary buckwheat germplasms

Muhammad Rauf,윤혜명,이수경,현도윤,이명철,오세종,최유미 한국육종학회 2019 Plant Breeding and Biotechnology Vol.7 No.4

Buckwheat sprouts are used as a functional food in several countries owing to their soft and slightly crispy texture andattractive fragrance. They possess excellent anti-oxidant activity and are rich in amino acids, minerals, and flavonoids; however, sproutgrowth and flavonoid content may vary among buckwheat germplasms. The present study was performed to evaluate 87 common and13 tartary buckwheat germplasms for sprout growth traits and rutin and quercetin content. We found that the common type buckwheatsprouts had considerably higher leaf length and width, fresh shoot weight, and extract weight than those of tartary buckwheat sprouts,but the rutin and quercetin content was significantly higher in tartary buckwheat sprouts. The rutin content ranged from 323.7 to 750.6 mg/100 g DW in common buckwheat sprouts and 2220 to 3185 mg/100 g DW in tartary buckwheat sprouts. Similarly, the quercetin contentranged from 0.27 to 1.82 and 4.76 to 30.41 mg/100 g DW in common and tartary buckwheat sprouts, respectively. Additionally, therewas no significant correlation between the growth traits and rutin content in common buckwheat sprouts, whereas, the leaf length oftartary buckwheat sprouts showed a significant positive correlation with the rutin content. This information can be used by plantbreeders and plant geneticists to study the genetic and genomic approaches for buckwheat breeding program.

Assessment of Phenolic Content, Saponin Content, and Antioxidant Activities in Gray, Red, and White Adzuki Bean Germplasm: A Multivariate Analysis

Kebede Taye Desta,윤혜명(Hyemyeong Yoon),신명재(Myoung-Jae Shin),이수경(Sukyeung Lee),Xiaohan Wang,최유미(Yu-Mi Choi),전영아(Young-ah Jeon),주영광(YoungKwang Ju),이정윤(JungYoon Yi) 한국작물학회 2023 한국작물학회지 Vol.68 No.3

Seed color is controlled by several genes and is a key trait in determining the metabolite content and biological activities of legume genotypes. In this study, 296 adzuki bean accessions, including 159 grey, 99 red, and 38 white adzuki beans, were grown in Korea. Variations in total phenolic content (TPC), total saponin content (TSC), DPPH• scavenging activity, ABTS•+ scavenging activity, and ferric reducing antioxidant power (FRAP) were assessed and were reported to be in the ranges of 1.52- 8.24 mg GAE/g, 14.36-114.22 mg DE/g, 0.23-12.84 mg AAE/g, 1.05-17.66 mg TE/g, and 0.59-13.14 mg AAE/g, respectively, with a wide variation across adzuki beans. Except for DPPH• scavenging activity, the average values declined in the order gray > red > white adzuki beans, each demonstrating a significant variation (p < 0.05). White adzuki beans, which showed low metabolite content and antioxidant activity, were clearly separated from the gray and red genotypes using principal component and hierarchical cluster analyses. Moreover, TPC, TSC, and antioxidant activities were strongly correlated, regardless of seed color. Overall, the diversity of the TPC, TSC, and antioxidant activity in a broad population of adzuki bean genotypes was determined. Furthermore, this study found that seed color variation in adzuki beans had a significant effect on the metabolite content and antioxidant activity. Superior accessions with high levels of TPC, TSC, and antioxidant activity were also discovered and could be used for functional plant breeding and human consumption. The findings of this study may be useful for understanding the relationship between seed coat color and metabolite concentration in adzuki beans, paving the way for molecular-level analyses.

Variations of Yield Components and Anthocyanin Contents in Soritae and Yakkong Black Soybean Landraces Collected from Different Areas

최유미(Yu-Mi Choi),윤혜명(Hyemyeong Yoon),신명재(Myoung-Jae Shin),이윤정(Yoonjung Lee),이수경(Sukyeung Lee),Wang Xiao Han,Kebede Taye Desta 한국자원식물학회 2021 한국자원식물학회지 Vol.34 No.6

In this study, we cultivated 115 Soritae and 86 Yakkong black soybean landraces collected from ten different locations in Korea. Then, the variations of three yield components (one-hundred seeds weight (HSW), number of seeds per pod (SPP), and yield per plant (YPP)) and three anthocyanins (cyanidin-3-O-glucoside (C-3-O-G), delphinidin-3-O-glucoside (D-3-O-G) and petunidin-3-O-glucoside (Pt-3-O-G)) were investigated according to landrace type and collection area. Both yield components and anthocyanin contents significantly varied between the soybeans demonstrating genetic differences. Soritae landraces had the highest average HSW and TAC, whereas Yakkong landraces displayed the highest average SPP irrespective of collection area. Relatively, Yakkong landraces from Gyeongsangnam-do (1697.29 ㎎/100 g) and Soritae landraces from Gyeonggi-do (2340.94 ㎎/100 g) had the highest average TAC. Principal component analysis clearly separated Soritae and Yakkong landraces. Moreover, TAC and C-3-O-G showed positive and significant associations in both Soritae (r = 0.972) and Yakkong (r = 0.885) landraces, while yield components showed negative or weak correlations with each other. Overall, ten landraces were identified as important resources owing to their high yield (>150 g/plant) and high level of TAC (>2300 ㎎/100g). This study could lay foundations to molecular level investigations and reinforce the use of Yakkong and Soritae landraces during cultivar development.