안개 D형 저지원을 갖는 MLS 내성 황색 포도상 구균의 분포와 내성 기전

윤은정(Eun-Jeong Yoon),김현지(Hyunjee Kim),권애란(Ae-Ran Kwon),최성숙(Sung-Sook Choi),심미자(Mi-Ja Shim),최응칠(Eung-Chil Choi) 대한약학회 2006 약학회지 Vol.50 No.3

A new type of macrolides, lincosamides and streptogramin B antibiotics (MLS) resistance, showing the characteristic phenotype growing within the inhibition zone area around the clindamycin disk, was identified among clinically isolated Staphylococcus aureus. We named the phenotype as MLS resistance with foggy-D shape (fMLS). The average frequency of fMLS isolates was 9%. All of fMLS isolates have only erm(A) for the resistance determinant. The growth pat- tern of the challenged fMLS isolate looks intermediate phase between the patterns of inducible MLS resistance and constitutive MLS resistance.

시프로플록사신 내성 황색 포도상 구균에서 MIC의 하향 분포로 입증된 DW-224a의 in vitro, 항균 활성의 비교 우수성

윤은정 ( Eun Jeong Yoon ),이춘영 ( Chun Yeong Lee ),이종서 ( Jong Seo Lee ),최응칠 ( Eung Chil Choi ),심미자 ( Mi Ja Shim ) 韓國藥劑學會 2009 Journal of Pharmaceutical Investigation Vol.39 No.6

임상분리 호흡기 감염증 원인 균주에서의 Macrolide계 항생물질의 내성

윤은정,하장범,최응칠,심미자,Yoon Eun-Jeong,Ha Jang-Bum,Choi Eung-Chil,Shim Mi-Ja 대한약학회 2004 약학회지 Vol.48 No.6

The prevalence of resistance to a range of macrolides was determined for clinical isolates of common respiratory pathogens using NCCLS testing methods and interpretative criter ia.71.4% of Streptococcus pneumoniae, 62.3% of Staphylococcus aureus, 50.8% of coagulase-negative staphylococci and 4.4% of Strpetococcus pyogenes were erythromycin resistant. Also, the rates of resistance to other macrolides and clindamycin in these clinical isolates were as high as to eryth -romycin. Almost all of the macrolide-resistant isolates were positive for erm of the methylase gene, or mef of the efflux gene.

새로운 플루오로 퀴놀론계 항균제에 대한 내성 MRSA 균주의 In Vitro 선발과 그 내성 기전 분석

윤은정,김현지,이춘영,최응칠,심미자,Yoon, Eun-Jeong,Kim, Hyun-Jee,Lee, Chun-Yeong,Choi, Eung-Chil,Shim, Mi-Ja 대한약학회 2008 약학회지 Vol.52 No.3

Clinically isolated methicillin-resistant Staphylococcus aureus strains were exposed to subinhibitory concentration of DW286, DW-224a, gemifloxacin, trovafloxacin, sparfloxacin and ciprofloxacin during 26- to 39-days period. Subculturing led to resistance development, and most of the selected mutants were above susceptible breakpoints. Selected mutants had broad cross resistance to other quinolone antibiotics and only one mutant was completely susceptible to all fluoroquinolones. Twenty five among 42 mutants revealed mutations on DNA gyrase and topoisomerase IV by sequencing. Also 16 mutants had fluoroquinolones MICs that were 4-32 times lower in the presence of reserpine. In conclusion, alterations in DNA gyrase or topoisomerase IV and action of efflux pumping out system are the resistance mechanisms of DW-224a.

임상 분리 그람 양성 구균에 대한 MLS계 항생물질의 내성

윤은정(Eun-Jeong Yoon),윤종민(Jong-Min Yoon),최성숙(Sung-Sook Choi),권애란(Ae-Ran Kwon),심미자(Mi-Ja Shim),최응칠(Eung-Chil Choi) 大韓藥學會 2006 약학회지 Vol.50 No.3

A total of 398 strains of Staphylococcus aureus (99), coagulase-negative staphylococci (CNS) (99), enterococci (100), Streptococcus pyogenes (18) and Streptococcus agalatiae (82) were in Korean tertiary hospital from Dec. 2004 to Mar 2005. When minimal inhibitory concentrations (MIC), phenotypes and genotypes were determined, 70% of S. aureus, 65.7% of CNS, 78% of enterococci and 37.8% of S. agalatiae were resistant to erythromycin and 95.7% of erythromycin-resistant (EMR) S. aureus 92.3% of EMR CNS and all of EMR enterococci and S. agalatiae had erm of the methylase gene of msr(A) of the efflux gene.

MLS$_B$계 항생물질 유도 내성 세균에서 In vitro로 선발된 지속성 내성형 erm(A)와 erm(C)의 분자적 특성 규명

윤은정,진성혜,최응칠,심미자,Yoon, Eun-Jeong,Jin, Sung-Hye,Choi, Eung-Chil,Shim, Mi-Ja 대한약학회 2007 약학회지 Vol.51 No.2

The predominant Macrolides-Lincosamide-Streptogramin B (MLS$_B$) antibiotics resistance genes in staphylococci are erm(A) and erm(C). There is the phenomenon that the ratio of constitutively MLS$_B$ antibiotics resistance (cMLS) in erm(A) is much higher than in erm(C). Thus, we confirmed that the difference of the mutation ratio between erm(A) and erm(C) makes the phenomenon. We examined 8 staphylococci carrying inducibly expressed (iMLS) erm(A) or erm(C) genes. After overnight incubation in the presence of the non-inducer MLS$_B$ antibiotics, spontaneous mutants constitutively expressed MLS$_B$ resistance were selected. Against our expectation, the mutation ratio of erm(A) was lower than erm(C). Therefore, possibilities of other factors determining the ratio of cMLS phenotype might be concerned. All the mutants showed sequence alterations in translational attenuator and all the alterations seemed to give rise to change the second structure of mRNA to express constitutively. For erm(A), 4 different types of sequence deletions ranging from 72 bp to 122 bp and 3 different types of duplications ranging 24 bp to 93 bp were detected. Also, there were 9 different types of duplications ranging 15bp to 154bp in erm(C).

유아의 지능과 창의성과의 관계

이경화(Lee Kyung Hwa),윤은정(Yoon Eun Jeong) 한국영재교육학회 2004 영재와 영재교육 Vol.3 No.1

  본 연구에서는 발달의 민감기에 있는 유아들의 지능과 창의성 간의 관계를 살펴봄으로써 적절한 교육적 환경조성과 함께, 창의성 교육프로그램 개발을 위한 기초 자료를 제공하는데 목적을 두었다. 이를 위해 서울시에 소재한 SIGT 영재교육연구원에 내원한 유아 81명을 대상으로 지능검사(K-WPPSI)와 창의성 검사를 실시하였다.<BR>  연구 결과, 유아의 지능과 창의성 간에는 전체지능과 도형창의성, 창의적 성격에서 유의미 한 상관관계를 나타낸 반면에 언어창의성과는 상관이 없는 것으로 나타났다. 또한 유아의 지능수준에 따라 도형창의성에서 차이가 나타났으며 연령에 따라서는 동작성 지능의 도형영역 과 도형창의성의 완성도, 주제, 비관습성에서 유의미한 차이가 있었으며 창의적 성격은 호기심이 유의미한 차이를 보였다.<BR>  위와 같은 결과는 유아의 지능과 창의성의 균형적인 발달을 위해서는 지능과 창의성수준, 창의적 성격, 연령을 고려한 차별화된 교수-학습 방법과 프로그램 개발의 필요성에 대한 시 사점을 주었다.   This research aims to find relationships between intelligence and creativity among the children who are in a particularly sensitive stage of physical or mental development. From this, a basic data will be processed, which will be helpful for determining an educational condition and the creation of a relevant program.<BR>  First, there was a significant relationship in creativity of figure (P<.01) and characteristic creativity (P<.05) but there was very little in relation between creativity of language and intelligence. Second, there was a significant relationship between the creativity of figure (P<.01 and creativity according to the level of intelligence. It indicates that there were high levels of creativity of figure in talented children. However, there was no significant relation between characteristic creativity and the creativity of language. Nevertheless, there was as high a level of creativity as there was of high intelligence in all results. Third, there was partial difference between creativity and intelligence according to age. That means there was difference in figure area among the intelligence(P<.05) and there was significant difference within perfection, subjection and unconventionality(P<.001) of creativity of figure among the creativity. In addition there was particularly difference in curiosity(P<.01) among the characteristic of creativity.

Bacillus sp. HB-5 균주가 생산하는 단백 질 분해 효소의 분리. 정제 및 화장품에의 응용

이범천 ( Bum-chun Lee ),윤은정 ( Eun-jeong Yoon ),이동환 ( Dong-hwan Lee ),표형배 ( Hyeong-bae Pyo ) 대한화장품학회 2000 대한화장품학회지 Vol.26 No.1

A bacterial strain No. HB-5, which was capable of producing a protease in the culture conditions, was isolated from the soil. The protease was purified from cultural filtrate of Bacillus sp. HB-5 by membrane ultrafiltration and DEAE- cellulose chromatography, gel filtration on Sephadex G-100. The molecular weight was estimated to be 60Kda. The optimal pH and temperature for the activity of the purified protease pH were 11 and 50°C, respectively. The enzyme was stable within a pH range 8-12 and up to 60°C. The enzyme activity was highly inhibited by PMSF at 1mM. The proteolytic actions of protease and papain on human epidermis keratins which are major protein impurities on the skin, were compared. The bacterial protease degraded more effectively than papain. Product containing 2% protease exhibited 21% increase on the skin coloration index. These results suggest that cosmetic product containing protease produced by Bacillus sp. HB-5 could remove the adherent keratin layer and then make a softer skin.

전북지역 도축돈 적내장 육안병변 조사

추금숙 ( Keum Suk Chu ),윤은정 ( Eun Jeong Yoon ),유기홍 ( Ki Hong You ),하용수 ( Yong Su Ha ) 한국동물위생학회 2014 韓國家畜衛生學會誌 Vol.37 No.3

Respiratory diseases are common in swine industry and have great economic importance. Respiratorydisorders cause substantial losses to the swine industry. Losses associated with respiratory disease vary considerably between herds and seasons. In a survey was carry out to investigate the lesion of red internal organs in slaughtered pigs and provided assistant data which are useful for each farm. From november 2012 to december 2013, 1,680 pigs out of 84 farms were sampled in Jeonbuk province. Gross lesions such as swine enzootic pneumonia (SEP), pleuritis, pleuropneumonia, pericarditis, liver milk spot were examined for the pigs. Overall prevalence of SEP was 55.7%. According to season, the incidence occurred higher in fall than winter, spring, and summer. The mean SEP score was 0.91, the highest incidence occurred in fall. The prevalence of pleuropneumonia, pleuritis, pericarditis, and milk spot was 36.4%, 49.7%, 2.3%, and 8.8%, respectively. The positive rate of PRRS and PCV2 was 2.9% and 70.0% by PCR analysis.

시각장애인을 대상으로 한 음악 연구의 동향

박혜영(Hye Young Park),조미란(Mi Ran Cho),윤은정(Eun Jeong Yoon) 한국콘텐츠학회 2018 한국콘텐츠학회논문지 Vol.18 No.6

본 연구는 시각장애인을 대상으로 한 음악 연구의 동향을 살펴보기 위해 1998년부터 2017년까지 국내 · 국제 학술지에 게재된 총 62편의 학술논문을 내용분석 하였다. 발표연대, 학술지 분야, 연구방법, 연구유형, 연구주제, 연령, 장애특성을 분석틀로 하였다. 연구결과, 최근 10년간 국내 · 외 모두 논문 편수가 급증하였으며, 교육학, 치료학 등 다양한 분야로 확대되는 경향을 보였다. 연구방법에서 국내는 조사연구가 압도적으로 많은 반면, 국외는 질적연구와 실험연구가 고르게 분포하였다. 연구유형과 주제를 살펴보면, 국내는 음악흥미와 적성을 주제로 한 인식 및 현황조사 연구가 다수였으나, 국외는 음악중재, 음악지각 및 인지를 주제로한 음악의 영향과 경험 연구가 높은 빈도를 나타냈다. 연령은 국내 · 외 모두 성인과 아동청소년으로 조사되었고 노인 대상은 확인되지 않았다. 장애특성별 빈도에서 전맹, 저시력을 대상으로 한 연구가 많은 반면, 중도나 중복장애는 적었다. 이는 시각장애와 음악에 관한 연구 동향을 설명함으로써 해당 분야 후속연구의 방향을 제안한다는 의의를 갖는다. The purpose of this study was to perform a content analysis on 62 domestic and international journals from 1998 to 2017, in order to review research trends in music and people with visual impairments. The framework of the content analysis was based on the year of publication, field of journal, research method, type of research, research subject, participants’ age, and factors of impairment. The results showed that both domestic and international journals have rapidly increased in the last 10 years, and have expanded into the fields of education and therapy. In the research method, survey research was conducted much more frequently in domestic journals, while both qualitative and experimental research were employed on a roughly even basis in international journals. With respect to the type and subject of research, music perception and recent trends on the subject of musical interest and aptitude took up the majority of domestic journals, while international journals focused on music intervention, music perception and cognition with musical influences and experiences. The participants in both domestic and international journals were children, adolescents and adults, while the elderly were not observed. In the factors of impairment, research on people with total blindness or low vision were conducted frequently, but those with adventitious vision loss or multiple impairments were rarely performed. This study could be meaningful to suggest the direction of further studies, through the investigation of research trends in music and people with visual impairments.