고지방식이(高脂肪食餌)로 유발(誘發)된 비만백서(肥滿白鼠)모델에서 창출(蒼朮), 의이인(薏苡仁), 마황(麻黃), 창출합의이인(蒼朮合薏苡仁) 마황합록차(麻黃合綠茶) 약침(藥鍼)이 혈청지질(血淸脂質) 및 간기능(肝機能)에 미치는 영향(影響)

윤대환,강자돈,주준성,채우석,나창수,Youn, Dae-Hwan,Kang, Ja-Don,Joo, Joon-Sung,Chae, Woo-Seok,Na, Chang-Su 대한약침학회 2004 Journal of pharmacopuncture Vol.7 No.1

Objective : This research was performed to investigate the effect of herbal acupuncture(Atratylodes japonica, Coix lachrymajobi, Ephedra sinica, Atratylodes japonica mixed with Coix lachrymajobi and Ephedra sinica mixed with Green tea) at Pungnyung(ST40) and Umnungchon(SP9) on weight gain, food intake, food efficiency, serum of lipid concentrations, liver function and HDL to total cholesterol ratio of rats fed high fat diet for 5weeks. Method : Experimental groups were divided into normal group(Normal), high fat diet group(Control), high fat diet and Atractylodes japonica-herbal acupuncture group(AJ), high fat diet and Coix lachrymajobi-herbal acupuncture group(CL), high fat diet and Ephedra sinica-herbaI acupuncture group(ES), high fat diet and Atractylodes japonica+Coix lachrymajobi-herbal acupuncture group(AJ+CL), Ephedra sinica+Green tea-herbal acupuncture group(ES+GT). Herbal acupuncture was bilaterally treated at the level of 132.5mg/kg body weight per 2day. Results : Body weight and food efficiency were decreased in AJ, ES, AJ+CL, ES+GT. The level of serum total cholesterol, triglyceride and free fatty acid were increased in AJ, ES, ES+GT. That of serum HDL-cholesterol was increased in AJ. The change of food intake, the level of serum phospholipid and ALP were not significant. The HDL to Total cholesterol ratio was increased in AJ and ES. Conclusion : Atractylodes japonica-herbal acupuncture in ST40 SP9 is effective on Body weight, food efficiency ratio, the level of serum lipid, protection of liver function and prevention cardiovascular risk by obesity induced by high fat diet. Herbal acupuncture mixed Ephedra sinica with Green tea can control the body weight, food efficiency ratio and the level of serum lipid.

高脂肪食餌로 誘發된 肥滿白鼠모델에서 저용량 레이져 요법이 血淸脂質 및 肝機能에 미치는 影響

윤대환,이유경,김성철,최동희,나창수 경락경혈학회 2004 Korean Journal of Acupuncture Vol.21 No.4

Objectives : Laser therapy started in 1958 when Schawlow and Townes suggested medical value of Laser therapy. He-Ne laser has been utilized as a clinical treatment for various diseases by Plog since 1975. Low Level Laser Therapy (LLLT) has been used as medication for controlling obesity in the Korean Medicine. So this study is planned to investigate the effects of LLLT on the level of serum lipid and weight gain Methods : Experimental groups were divided into normal group(Normal), high fat diet group(Control), high fat diet and LLLT by helium-neon (He-Ne) on the tail is carried out once a 2 day during 5 weeks. The animals were divided into six groups: no ischemia-induced and no LLLT-treated group (Normal), the ischemia-induced and no LLLT-treated group (Control), the ischemia-induced and 5 mW 5 min LLLT-treated group (LLLT5-5), the ischemia-induced and 30 mW 5 min LLLT-treated group (LLLT30-5), the ischemia-induced and 5 mW 10 min LLLT-treated group (LLLT5-10), 30 mW 10 min LLLT-treated group (LLLT30-10). The effect of LLLT is observed by weight gain, food intake, food efficiency, serum of lipid concentrations, liver function and HDL to total cholesterol ratio of rats fed high fat diet for 5weeks. Results : Body weight and food intake were decreased in LLL5-5, LLLT30-5, LLLT30-10. Food efficiency was decreased in LLLT30-10. The level of serum Triglyceride, Free fatty acid, AST, ALT, ALP were decreased in LLLT30-10. Serum HDL-cholesterol was increase in LLLT5-10, LLLT30-10. Also serum ALT was decrease in LLLT5-5Conclusions : LLLT(30 mW-10 min) is effective on Body weight, food efficiency ratio, the level of serum lipid and protection of liver function by obesity induced by high fat diet, and LLLT(5 mW-5 min) act on decrease of Body weight, food intake and ALT

足三里 鍼刺가 人體의 Skin temperature, Galvanic skin response 및 Heart rate에 미치는 影響

윤대환,나창수 경락경혈학회 2003 Korean Journal of Acupuncture Vol.20 No.3

大敦·湧泉 捻轉 및 迎隨 補瀉手技法이 2K1C에 의하여 誘發된 高血壓 白鼠에 미치는 影響

윤대환,한정희,나창수,김선희 경락경혈학회 2007 Korean Journal of Acupuncture Vol.24 No.3

Objectives : The purpose of this study was to examine effects of acupuncture by needle manipulation at LR1KI1 on the blood pressure, plasma levels of ANP(atrial natriuretic peptide), renin and cardiomegalic index in hypertensive rat induced by two kidney one clip (2K1C).Material and methods : The groups divided into 7 groups; Control, no treatment. Acu-1, acupuncture at LR1KI1 bilaterally and the needle was twirled and rotated forward with the thumb of the right hand 6 times. Acu-2, acupuncture at LR1KI1 bilaterally and the needle was twirled and rotated forward with the forefinger of the right hand 6 times. Acu-3, acupuncture at LR1KI1 bilaterally and the needle was inserted in the opposite direction(body direction) as the channel runs, Acu-4, acupuncture at LR1KI1 bilaterally, the needle was inserted in the opposite direction(body direction) as the channel runs and the needle was twirled and rotated forward with the forefinger of the right hand 6 times. The acupuncture treatments by needle manipulation were started on the 4 week after inducing 2K1C, and executed 5times for 2 weeks. Results : Systolic blood pressure was decreased significantly in Acu-2, Acu-3 and Acu-4. Plasma renin was decreased significantly in Acu-2 and Acu-4. Plasma ANP was increased significantly in Acu-2, however was decreased significantly in Acu-4. Cardiac hypertrophy index was decreased significantly in Acu-1, Acu-2, Acu-3 and Acu-4.Conclusions : These results suggest that acupuncture by needle manipulation at LR1KI1 is effective in hypertension by renal blood problem.

三陰交, 陰陵泉, 膀胱兪 單獨 및 配穴 艾灸 施術이 Uranyl Acetate에 의하여 誘發된 白鼠의 急性腎不全에 미치는 影響

윤대환,박종승,김용성,김정상,나창수 경락경혈학회 2003 Korean Journal of Acupuncture Vol.20 No.2

直刺, 迎隨 및 捻轉手技法에 따라 시행한 經渠·復溜 鍼刺가 中大腦動脈 閉塞에 의하여 誘發된 局所 腦虛血 白鼠 hippocampus의 抗細胞自滅死 및 神經保護에 미치는 影響

윤대환,최찬헌,백진웅,정연진,나창수,정지연,변정윤 경락경혈학회 2007 Korean Journal of Acupuncture Vol.24 No.3

足三里의 竹瀝 藥鍼이 Intraluminal Filament 揷入術에 의하여 誘發된 白鼠의 虛血性 局所 腦損傷에 미치는 影響

윤대환,이봄비,심인섭,나창수 경락경혈학회 2005 Korean Journal of Acupuncture Vol.22 No.2

Objectives : Bambusae Caulis in Liquamen(BCL) has been used to medication for early stroke in the Oriental Medicine. So this study was planned to investigate the effects of BCL on the focal ischemia-induced by intraluminal filament insertion in the rats. Materials and methods : The focal ischemia was induced by Intraluminal Filament insertion into middle cerebral artery. BCL herbal acupuncture at ST36 was carried out during 3 weeks after focal ischemia-induced. Eight-arm radial maze was used for the behavioral task and neuroprotective effect of BCL acupuncture was observed by Cresyl violet, AchE, ChAT-stainResults : The error rate in the eight-arm radial maze task was significantly decreased in BCL1(0.89mg/kg) herbal acupuncture group compared to control group on 3,4,5days, compared to saline acupuncture group on 3,5days. The rate of correct choice was significantly increased in saline acupuncture, BCL1 herbal acupuncture group, BCL2(0.089mg/kg) herbal acupuncture groups compared to control group. The density of neurons in the hippocampal CA1 was the most increased in BCL1 herbal acupuncture group compared to the others. The density of AchE in the hippocampal CA1 was significantly increased in BCL1 herbal acupuncture, BCL2 herbal acupuncture groups compared to control group, and in BCL2 herbal acupuncture groups increased compared to saline acupuncture, control groups. The density of ChAT in the hippocampal CA1 was significantly increased in BCL1 herbal acupuncture group compared to saline acupuncture, control groups. Conclusions : These results suggest that BCL herbal acupuncture could be used as a medication for controlling the early stroke

대돈(大敦)${\cdot}$용천(湧泉) 염전(捻轉) 및 영수(迎隨) 보사수기법(補瀉手技法)이 2K1C에 의하여 유발(誘發)된 고혈압(高血壓) 백서(白鼠)에 미치는 영향(影響)

윤대환,한정희,김선희,나창수,Youn, Dae-Hwan,Han, Jeong-Hee,Kim, Suhn-Hee,Na, Chang-Su 경락경혈학회 2007 Korean Journal of Acupuncture Vol.24 No.3

Objectives : The purpose of this study was to examine effects of acupuncture by needle manipulation at $LR1{\cdot}Kl1$ on the blood pressure, plasma levels of ANP(atrial natriuretic peptide), renin and cardiomegalic index in hypertensive rat Induced by two kidney one clip(2K1C). Material and methods : The groups divided into 7 groups; Control, no treatment. Acu-1,acupuncture at $LR1{\cdot}Kl1$ bilaterally and the needle was twirled and rotated forward with the thumb of the right hand 6 times. Acu-2, acupuncture at $LR1{\cdot}Kl1$ bilaterally and the needle was twirled and rotated forward with the forefinger of the right hand 6 times. Acu-3, acupuncture at $LR1{\cdot}Kl1$ bilaterally and the needle was inserted in the opposite direction(body direction) as the channel runs, Acu-4, acupuncture at $LR1{\cdot}Kl1$ bilaterally, the needle was inserted in the opposite direction(body direction) as the channel runs and the needle was twirled and rotated forward with the forefinger of the right hand 6 times. The acupuncture executed 5times for 2 weeks. Results : Systolic blood pressure was decreased significantly in Acu-2, Acu-3 and Acu-4. Plasma renin was decreased significantly in Acu-2 and Acu-4. Plasma ANP was increased significantly in Acu-2, however was decreased significantly in Acu-4. Cardiac hypertrophy index was decreased significantly in Acu-1, Acu-2, Acu-3 and Acu-4. Conclusions : These results suggest that acupuncture by needle manipulation at $LR1{\cdot}Kl1$ is effective in hypertension by renal blood problem.

족삼리(足三里) 침자(鍼刺)가 인체(人體)의 Skin temperature, Galvanic skin response 및 Heart rate에 미치는 영향(影響)

윤대환,나창수,Youn Dae-Hwan,Na Chang-Su 경락경혈학회 2003 대한침구의학회지 Vol.20 No.3

Objectives : The purpose of this study was to investigate the Effect of Acupuncture Chok-samni(ST36) on Skin temperature(ST), Galvanic skin response(GSR) and Heart rate(HR) in humans. ST36 is used in uneasiness, high fever, stroke, paralysis on oriental medicine. Methods : Subjects were 18 healthy oriental medical students volunteers(15male, 3female, mean age-28). Under sequence of ST36 acupuncture, the first group was observed ST, the second group was observed GSR and the third group was observed HR. Skin temperature was measured on Haegye acupoint(ST41) before and after acupuncture stimulation. Each test took 15minutes. This test was performed twice in order to increase the statistical authenticity. Results : Our result indicates that acupuncture stimulation of ST36 statistically decreased skin temperature on ST41. Also statistically decreased in HR. The change of GSR after ST36 acupuncture was decreased, but there is no statistical. Conclusions : These results suggested that acupuncture stimulation of ST 36 has effect on ST and HR in human body, and may be used by treatment as control the nerve system.

合谷 復溜 補瀉 施術이 少汗에 미치는 影響(Ⅱ)

윤대환,나창수,최찬헌,김영선,김왕인 경락경혈학회 2010 Korean Journal of Acupuncture Vol.27 No.2

Objectives : The purpose of this study is to identify the effect of tonifying or sedative manipulation on LI4 and KI7 for sweet deficiency under cold environment. Methods : The participants were divided into 4 groups; intact group without acupuncture(Intact), acupuncture group inserting straightly with LI4 & KI7 method((LI4-straight⋅KI7-straight), acupuncture group inserting⋅twisting with LI4 tonificaton & KI7 tonificaton method(LI4-tonify ⋅KI7-tonify) and acupuncture group inserting⋅twisting with LI4 sedation & KI7 sedation method(LI4-purge⋅KI7-purge). We obtained the baseline data under cold condition with the temperature 14±1℃. After making the participant staying under cold condition for 10 minute, we measured sweating rate on skin surface and biological responses such as body temperature, oxygen saturation, pulse rate, systolic blood pressure, diastolic blood pressure, deoxy-Hb and oxy-Hb in subcutaneous. Results : The sweating rate on skin surface was not different in all treatment groups compared to the intact group. The body temperature was significantly increased in LI4-straight⋅KI7-straight group compared to the intact group. The oxygen saturation significantly increased in LI4-purge⋅KI7-purge group compared to the intact group. The systolic blood pressure was significantly increased in LI4-purge⋅KI7-purge group compared to the intact group. The deoxy-Hb was significantly decreased in LI4-purge⋅KI7-purge group compared to the intact group. Conclusions : The above results, the effect of LI4, KI7 acupuncture due to the tonifying or sedative manipulation on sweating control could be observed. However, other biological responses with the acupuncture of tonifying or sedative manipulation were giving different expressions. We suggest that continued research of tonifying or sedative manipulation is of importance order to find these expressions.