http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : 유지헌(Ji Heon Yu) (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
-
정향과 마가목 복합물의 in vitro와 in vivo 항비만 효과 연구
유지헌(Ji Heon Yu),안희연(Hui Yeon An),노성수(Seong-Soo Roh),신미래(Mi-Rae Shin) 한국영양학회 2024 Journal of Nutrition and Health Vol.57 No.2
본 연구에서는 정향과 마가목 복합물 (SS)의 항비만 효과를 알아보기 위해 실험을 진행하였다. SS 투여는 3T3-L1 세포 내 TG와 TC가 유의적으로 감소하는 효과를 나타냈으며, 지질 합성관련 유전자와 지방산 산화 관련 유전자 발현을 조절하는 효과를 보여주었다. 비만이 유도된 C57BL/6 mice에서 SS 투여는 혈청 내 leptin 호르몬 수치를 감소시켰으며, AMPK/ACC/SREBP-1 경로를 경유하여 TG 합성을 억제하였다. 또한, 조직병리학적 분석을 통해 지질 축적과 지방세포의 크기가 감소된 것을 확인하였다. 따라서 SS는 비만의 예방과 치료를 위한 잠재력을 갖춘 소재로 사료된다. Purpose: This study investigated the anti-obesity effects of a combination of Syzygium aromaticum L. and Sorbus commixta Hedl. (SS) in vitro and in vivo. Methods: The extracts of Syzygium aromaticum extract (SA) and Sorbus commixta extract (SC) were prepared individually using distilled water. They were mixed in a 1:2 ratio for use in the experiment. To assess the anti-obesity potential of SS in vitro, we examined cell proliferation, cellular triglyceride (TG), and total cholesterol (TC) levels, as well as lipogenesis and β-oxidation in 3T3-L1 cells. To confirm its anti-obesity potential in vivo, C57BL/6J mice were fed a 60% high-fat diet (HFD) to induce obesity. SA alone, SC alone, and their combination compound, SS (at a dosage of 200 mg/kg) were orally administered for 6 weeks. Thereafter, to conduct a comparative evaluation, serum analysis, western blotting of liver tissues, and histopathological analysis were performed. Results: Both SS200 and SS400 significantly inhibited the cellular TG and TC contents in the 3T3-L1 cells. Furthermore, treatment of the cells with SS (at a dose 200 and 400 μg/mL) also led to a noticeable regulation of key lipogenic and β-oxidation factors. Treatment of obese mice with SS resulted in a greater reduction in serum leptin and TG levels compared to treatment with the individual compounds (SA and SC). Furthermore, activation of AMPactivated protein kinase α by SS treatment resulted in the suppression of sterol regulatory element-binding proteins (SREBP)-1, leading to the inhibition of acetyl-CoA carboxylase (ACC) expression. Conclusion: Our results suggest that SS may have the potential to prevent obesity through a reduction in the TG and TC levels and regulation of lipogenesis and β-oxidation.
-
H₂O₂ 유도 U2OS 세포에서 Pinus sylvestris L.의 산화적 스트레스 및 골관절염 개선 효과
신미래(Mi-Rae Shin),최정원(Jeong Won Choi),김민주(Min Ju Kim),안희연(Hui Yeon An),유지헌(Ji Heon Yu),정일하(Il Ha Jeong),유왕근(Wang Keun Yoo),Bold Sharavyn,노성수(Seong-Soo Roh) 한국식품영양과학회 2024 한국식품영양과학회지 Vol.53 No.5
This study investigated the pharmacological impact of the Pinus sylvestris L. water extract (PS) on hydrogen peroxide (H2O2)-induced oxidative stress in the human osteosarcoma cell line U2OS and a monosodium iodoacetate (MIA)-induced osteoarthritis model in rats. A MitoSoxTM indicator was used to measure mitochondrial reactive oxygen species (ROS) levels, while mitochondrial integrity was assessed using MitoTrackerTM. Moreover, we assessed oxidative stress-related markers, including sirtuin 1 (Sirt1), metal-responsive transcription factor 1 (MTF1), and 4-hydroxynonenal (4-HNE) using immunohistochemistry. We performed serum analysis and western blotting in vivo. The cell viability of U2OS cells was increased in a concentration-dependent manner, independent of H2O2 treatment. PS dramatically reduced the accumulation of mitochondrial ROS, while simultaneously reversing the decreased MitoTrackerTM levels. Additionally, both Sirt1 and MTF1 intensities were significantly elevated, whereas 4-HNE intensity noticeably decreased. In the animal experiment, the MIA treatment resulted in a reduction of hindpaw weight distribution changes. However, PS pretreatment significantly reversed this reduction. The serum levels of tumor necrosis factor-alpha, interleukin (IL)-6, and IL-1β were significantly decreased compared to the control group. The control group, treated with only MIA, exhibited increased levels of three enzymes: Matrix metalloproteinase 13 (MMP- 13), which degrades collagen or proteoglycan, and MMP-2 and MMP-3, which degrade the non-collagen matrix components of the joints. However, the PS treatment significantly inhibited the levels of the enzymes except MMP-3. In conclusion, these results suggest that PS alleviated oxidative stress and regulated inflammatory cytokines and the expression of MMPs.
ScienceON
DBpia연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
