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인삼(人蔘)(Panax ginseng)의 종자형성(種子形成)에 따른 배유세포(胚乳細胞)의 딕티오좀 및 Spherosome 형성
유성철,김우갑,Yu, Seong-Cheol,Kim, Woo-Kap 한국현미경학회 1991 Applied microscopy Vol.21 No.2
This study has been carried out to investigate the development of dictyosome, and roles of dictyosome about the formation of spherosome in the endosperm cell during seed formation of Panax ginseng with electron microscope. The result is as follows; In the endosperm cells of early stage during seed formation of Panax ginseng, plastid, mitochondria, endoplasmic reticulum, dictyosome and ribosomes are evenly distributed in cytoplasm. Electron lucent vesicles derived from dictyosome are observed in endosperm cells. Vesicles that contain low electron density are derived from forming face of dictyosome and releases into the cytosol. This vesicles formed multi vesicular body or fused with the plasma membrane. The spherical spherosomes are formed from dictyosome containing the lipid materials of even electron density and are gradually increased in size and number. Dictyosome is located in between vacuole and spherosome and it's cisternae form a semicircle and a circle. Some membrane of the protein body that accumulate the storage protein are originate from the spherical vacuole which interfused between vesicles and vacuoles derived from dictyosome.
인삼(Panax ginseng C.A. Meyer) 배유세포의 Protein Body 형성에 관한 연구
유성철,정병갑,김우갑,Yu, Seong-Cheol,Jeong, Byung-Kap,Kim, Woo-Kap 한국현미경학회 1988 Applied microscopy Vol.18 No.2
The developmental processes of the protein body are studied on endosperm cells of Panax ginseng during seed maturation periods. The spherosome, mitochondria, rough endoplasmic reticulum, and ribosome are observed and then are gradually increased in early endosperm cells. Protein body developed from vesicles produced by the rough endoplasmic reticulum and was formed at the enlarged ends of rough endoplasmic reticulum. Also, vacuole-like protein body was observed in associated with rough endoplasmic reticulum. Golgi complex is observed in associated with vacuole and its vesicles containing proteinaceous granules moved and accumulated to the vacuole. Proteinaceous granules are deposited in the spherical or oval shaped vacuole and gradually, vacuole is surrounded by the multi-membranous structure. Rough endoplasmic reticulum, ribosome, Golgi complex, and vacuole are observed in associated with protein body formation.
유성철,허환,김준규 대한미용성형외과학회 2008 Archives of Aesthetic Plastic Surgery Vol.14 No.2
Telangiectasis is defined as permanent visible dilatation of the cutaneous blood vessels. Clinically, its diameter ranges from 0.1 to 1.0mm which represents either an expanded venule, an capillary, or sometimes an arteriole on the face. Until now, many treatment methods have been reported such as various laser therapy, sclerotherapy, electrodessication and so on. We used superpulse CO₂ laser to remove nasal telangiectasis in 8 patients between May 2004 and July 2007. Laser therapy was done in continual 5 sessions with intervals of 3-5 weeks. In each session, laser beam was shot to the point of the bifurcation, trifurcation of telangiectatic vessels or to the points of the widest diameter. In each point, shots of the laser beam were repeated 5 times to reach deep dermis. The laser setting was 7W with 0.5mm diameter in superpulse mode. Photographs were taken before and after procedures to ascertain the changes of nasal telangiectasis. Our results showed that the main lesions were cleared effectively. We think that irradiation of CO₂ laser onto the bifurcating points can be a valuable tool to treat nasal telangiectasis.
전자현미경을 이용한 인삼종자 배유세포내의 지질 및 지질가수분해 효소의 분포
유성철,노미전 고려인삼학회 1992 Journal of Ginseng Research Vol.16 No.2
This study was carried out to investigate the localization of lipids and lipase activity with lipid staining and cytochemical technique in endosperm cells of Panax ginseng C.A. Meyer seed. In endosperm cells of indehiscent seed, protein bodies facing the umbiliform layer are different in electron density during the various degraded processes. Gradually, protein matrix near the cell wall was lysed and electron lucent inclusions appeared on umbiliform layer. The protein body with high electron density and the spherosome with low electron density were observed in endosperm cells. As a result of lipid staining, electron density of spherosome is more intense than those of the protein matrix within the protein body in endosperm cells of indehiscent seed. Free spherical spherosomes within the umbiliform layer have a high electron density. The spherical spherosomes were more electron densed and were uniform in comparison with the cytoplasmic proteinaceous granules in endosperm cells of seed with red seed coat. The major component of spherosome was determined to be lipid. Lipase activity occurs in the spherosome and near the endosperm cell wall facing the umbiliform layer. Cytochemical reaction products of lipase were observed in the spherosome membrane and in the inner regions of spherosome. After protein bodies were digested, lipase activities were observed in free spherosomes and near the cell wall of endosperm cells. Umbiliform layer composing of fibrillized wall and digested materials of the endosperm cell showed a little lipase reaction products.
An Algorithmic Approach to Total Breast Reconstruction with Free Tissue Transfer
유성철,Grant M. Kleiber,David H. Song 대한성형외과학회 2013 Archives of Plastic Surgery Vol.40 No.3
As microvascular techniques continue to improve, perforator flap free tissue transfer is now the gold standard for autologous breast reconstruction. Various options are available for breast reconstruction with autologous tissue. These include the free transverse rectus abdominis myocutaneous (TRAM) flap, deep inferior epigastric perforator flap, superficial inferior epigastric artery flap, superior gluteal artery perforator flap, and transverse/vertical upper gracilis flap. In addition, pedicled flaps can be very successful in the right hands and the right patient, such as the pedicled TRAM flap, latissimus dorsi flap, and thoracodorsal artery perforator. Each flap comes with its own advantages and disadvantages related to tissue properties and donor-site morbidity. Currently, the problem is how to determine the most appropriate flap for a particular patient among those potential candidates. Based on a thorough review of the literature and accumulated experiences in the author’s institution, this article provides a logical approach to autologous breast reconstruction. The algorithms presented here can be helpful to customize breast reconstruction to individual patient needs.