마황(Ephedra sinica) 에탄올추출물의 항산화 효과 및 각질세포 보호효과

유민정 ( Min Jeong Ryu ),모정희 ( Jeong Hee Mo ) 한국미용학회 2014 한국미용학회지 Vol.20 No.4

This study induced oxidative stress in human HaCat cells to test the antioxidativity of Ephedra sinica ethanol extract and its protectiveeffects in order to develop safer and more outstanding skin care materials and products from natural sources. Ephedra sinica ethanol extract has a polyphenol content of 175.5±0.66 μg/ml, flavonoid content of 56.91±2.15 μg/ml. As a result of measuring the DPPH radical absorption capacity, IC50 was 27.51 μg/ml, which was higher than that for BHT, a synthetic antioxidant, at 105.1 μg/ml, and it was above90% at 400 μg/ml, which is similar to that of vitamin C. With respect to the cell survival rates, when the extract was applied to HaCaTcells according to each concentration, cytotoxity was observed depending on the concentration. When the cell survival rate of the control group was considered as 100%, the test group which did not have any pre-treatment showed a cell survival rate of 49.5%. When the cell was stimulated with H2O2, ROS was removed by 81.5%, 76.0%, and 66.2%, which indicates its removal effect depending on concentration. The protective effect of the extract on HaCat cells from H2O2, rotenone and paraquat was checked and observed depending on concentration. Therefore, as this study discovered the antioxidativity of Ephedra sinica ethanol extract, its ROS removal from HaCat cells and the protective effect from oxidative stress, it is suggested that it will restore oxidative damage in skin.

노루발풀 추출물의 in vitro에서의 항산화, 항염증 및 항히스타민 활성

유민정 ( Min Jeong Ryu ),이숙영 ( Sook Young Lee ),차영주 ( Young Ju Cha ) 한국미용학회 2012 한국미용학회지 Vol.18 No.3

Pyrola japonica in Chinese traditional medicine have been used as tonics, sedatives, analgesics against rheumatoid arthritis, hemostatics, and recently isolated anti-inflammatory agents from whole plants. We have investigated antioxidant effect of P. japonica extract using DPPH raidcal scavenging test, total polyphenol and flavonoid content assay, nitrite(NO) scavenging test in cell-free system. Also, antihistamine activity by β-hexosaminidase assay and anti-inflammatory effect by inhibition rate of NO production in RAW 264.7 cell was evaluated. Skin irritation test of the extracts was evaluated in accordance with the guidelines of patch test. Plant extracts were prepared by ethanol extraction method. DPPH raidcal scavenging activity of ethanol extract of P. japonica. was the highest in 400 μg/mL as 88.62±1.51% and increased in a concentration-dependent manner. Total polyphenol and total flavonoid content of the extract was 107.5±0.03 μg/mL and 50±0.01 μg/mL, respectively. The NO scavenging activity in cell-free system was the highest in 800 μg/mL as 42.13±2.72% and it was also increased in a concentration-dependent manner. P. japonica extract will not significantly affect cell viability of RBL-2H3 cell for β-hexosaminidase assay and RAW 264.7 cell for NO content assay at the concentration of 5~50 μg/mL. The inhibition of β-hexosaminidase release was respectively indicated as 15.5, 43.0, 56.4, 67.9% in 5, 10, 20, 50 μg/mL of extract and it was increased in a concentration-dependent manner. Anti-inflammatory activity by NO content measurement in RAW cell was the highest in 50 μg/mL (NO content of 5.7±0.5 μM/mL) of P. japonica extract and additionally it was concentration-dependent. The patch application performed in order to determine dermal irritation of the extract showed result of no dermal reaction that is safety on skin. These results suggest that P. japonica extract can use as a resource of natural cosmetic material for anti-oxidant, anti-inflammatory, anti-itching gradients, and also safety on skin.

연구논문 : 오이풀 추출물의 비듬균에 대한 항진균 효과 및 RAW 264.7 세포에서의 항염증 효과

유민정 ( Min Jeong Ryu ),임요섭 ( Yo Sup Rim ),최다인 ( Da In Choi ),이숙영 ( Sook Young Lee ) 한국미용학회 2011 한국미용학회지 Vol.17 No.2

In this study, we analyzed the anti-fungal activity against Malassezia furfur, anti-oxidative activity and anti-inflammatory activity of Sanguisorba officinalis extracts. The ethanol extract of S. officinalis was sequentially fractionated with hexane, ethyl acetate, butanol and water. The cell viability was measured by MTT assay. Nitric oxide (NO) production was measured by Griess reagent assay. Anti-fungal activity was examined by paper disc method. The total phenolic contents and flavonoid contents were determined in all the samples, in the following order: ethanol > ethyl acetate > butanol > water > n-hexane. The ethyl acetate fraction showed high inhibition activity to DPPH scavenging radical activity at 500 μg/mL (96%) and more strongly inhibited NO productions compared with other fractions. Also, the ethyl acetate fraction showed the highest anti-fungal activity. According to the results above, it was summarized that the ethyl acetate fraction of S. officinalis have anti-oxidative, anti-fungal and anti-inflammatory activity in M. furfur or macrophage. It would be proposed that ethyl acetate fraction of S. officinalis can become a new cosmetic materials.

6종의 약용식물 추출물의 비듬균(Malassezia furfur)에 대한 증식억제 및 항산화 효과

유민정 ( Min Jeong Ryu ),이숙영 ( Sook Young Lee ),박열 ( Yeol Park ),양영기 ( Young Ki Yang ) 한국미용학회 2010 한국미용학회지 Vol.16 No.1

This study examined to screen the cosmetic materials with the antioxidative and antifungal activites of the crude extracts from 6 spp. medicinal plants. The extracts were prepared by ethanol extraction method. The antioxidant activities of extracts was used the method of DPPH radical and nitrite scavenging assay, total polyphenolics and flavonoid contents. Their antifungal activity against Malassezia furfur examined through the paper disc agar diffusion assay. Among the 6 plants tested, the extracts of Forsythia viridissima and Echinops setifer displayed a strong antifungal effect against M. furfur. The DPPH radical scavenging activity of the aerial part of Houttuyni cordata showed a considerable IC50 value (89.81 ㎍/㎖) and it was higher than that of BHT standard oxidant. The contents of total phenolics (292.36 ㎍/㎖) and flavonoids (208.20 ㎍/㎖) in the Houttuynia cordata aerial part were higher than the other medicinal plants. The nitrite scavenging ability was high in the extract of Forsythia viridissima fruit. These results suggested that medicinal plants could be the potential source of anti-dandruff agent.

연구논문 : 측백나무 잎 추출물의 미용 소재로서의 기능성 분석

정은숙 ( Eun Suk Jeong ),유민정 ( Min Jung Ryu ) 한국미용학회 2015 한국미용학회지 Vol.21 No.1

In this study, antioxidant (DPPH free radical scavenging activity, Nitric Oxide Scavenging activity), antimicrobial activities and bioactive materials (polyphenolics and flavonoids compounds) of ethanol extract of leaves from Thuja orientalis (TOL) were investigated. The ethanol extract from TOL showed higher DPPH radical scavenging activity and Nitric Oxide (NO) scavenging activity than other crude plant extract in a concentration-dependent manner. The present results suggest that the ethanol extract from TOL may be potent agent for antioxidant and thereby inhibit the pathological conditions caused by excessive generation of NO and ROS. Total phenolic content and Total flavonoid content was 61.2±2.35 and 42.32±1.015 μg/mL in ethanol extract of TOL respectively. The result of antimicrobialeffects, we were able to confirm that ethanol extract of TOL at S. epidermidis, p. acnes and M. furfur showed the clear zone on 250, 500 μg/disc concentration. The highest antimicrobial activity showed at p. ovale on 125, 250, 500 μg/disc concentration of ethanol extract of TOL. Taken together, ethanol extract of TOL was shown to have the most potent antioxidative and antimicrobial activities and the highest content of polyphenolic and flavonoids compounds.