고려인삼종자(高麗人蔘種子)의 발아특성(發芽特性)에 관(關)한 연구(硏究)

원준연,조재성,Won, Jun Yeon,Jo, Jae Seong 충남대학교 농업과학연구소 1988 Korean Journal of Agricultural Science Vol.15 No.1

This study was conducted to define the optimal conditions for embryo growth during seed stratification and for breaking dormancy as well as seed germination of stratified ginseng seeds. The experiments were also carried out to detect some materials which were expected to induce seed dormancy in the ginseng seeds. The results summarized as follows; 1. The growth of embryo during seed stratification was significantly inhibited by the existence of endocarp. The fastest embryo growth was resulted at $15^{\circ}C$ and an estimated optimal temperature for embryo growth was about $18^{\circ}C$. 2. There was no significant difference between the embryo growth and germination ratio of ginseng seeds which were sown in seed bed at Aug-5 without seed stratification and that of artificial seed stratification. 3. Embryo growth and germination ratio was significantly inhibited by high temperature treatment at $30^{\circ}C$ for 24 hours or respiration stress by immersing seeds in water for 10 days or more. 4. When the seed stratification was started at $10^{\circ}C$, growth of embryo in the ginseng seeds were almost stopped. But, when the seeds were stratified first at $20^{\circ}C$ for 50 days and next at $10^{\circ}C$ for 50 days, the embryo growth was significantly promoted compared with the embryo growth in the seeds which were stratified at $20^{\circ}C$ for 100 days. 5. The successive embryo growth after seed stratification was significantly accelerated at $10^{\circ}C$ but the seeds chilled at $5^{\circ}C$ for 100 days were resulted in the highest germination ratio as well as the shortest days for germination. 6. The successive embryo growth during chilling treatment and seed germination were significantly inhibited by immersing seeds in water just before chilling treatment or during chilling treatment and by interruption of chilling treatment with raising temperature to $20^{\circ}C$ for 20 days during chilling treatment. 7. The germination ratio of ginseng seeds which finished chilling treatment was highest at $10^{\circ}C$ and 62.5% was the estimated soil moisture for the best germination of ginseng seeds. The ginseng seeds were found to require high amount of oxygen for germination. 8. Only water soluble material in homogenized ginseng seeds showed a significant inhibiting effect on the seed germination of sesame, millet and soybean. Water soluble material dissolved from undehisced ginseng seeds showed stronger inhibiting effect on the seedling growth of sesame than material from dehisced ginseng seeds. Extraction temperature did not influence the inhibiting effect of the material dissolved from ginseng seeds on the seedling growth of sesame. 9. Water soluble materials dissolved from the berry pulps, leaves, fresh roots and dried roots also showed a significant inhibiting effect on the seedling growth of sesame. 10. Water soluble materials dissolved from the ginseng seeds, leaves and fresh roots showed a significant inhibiting effect on the germination of true fungi and the growth of spawn but the growth of phytopathogenic bacteria was not. 11. Among the water soluble materials dissolved from ginseng seeds, the materials of low molecular weight less than 3,000 were resulted a significant inhibiting effect on the seedling growth of sesame and the materials of high molecular weight also showed an inhibiting effect. 고려인삼종자(高麗人蔘種子)의 미숙배(未熟胚) 생장(生長) 최적조건(最適條件), 배숙종자(胚熟種子)의 유안타파(休眼打破) 및 발아최적조건(發芽最適條件)을 구명(究明)하는 한편 종자내(種子內)에 존재(存在)할 것으로 추측(推測)되는 발아억제물질(發芽抑制物質)을 추구(追究)하기 위하여 본(本) 연구(硏究)를 수행(遂行)하였던 바 그 결과(結果)를 요약(要約)하면 다음과 같다. 1. 내과피(內果皮)의 존재(存在)는 미숙배(未熟胚)의 생장(生長)을 현저(顯著)히 억제(抑制)하였으며 내과피(內果皮)의 유무(有無)에 관계(關係)없이 $15^{\circ}C$에서 배(胚)의 생장(生長)이 가장 왕성(旺盛)하였고 회귀분석(回歸分析)에 의한 배생장(胚生長) 최적온도(最適溫度)의 추정치(推定値)는 $18^{\circ}C$내외(內外)였다. 2. 수확직후(收穫直後) 미숙배종자(未熟胚種子) 파종시(播種時) 묘상(苗床)에서의 배생장(胚生長) 및 발아율(發芽率)은 개갑처리종자(開匣處理種子)의 배생장(胚生長) 및 발아율(發芽率)과 차이(差異)가 없었다. 3. 개갑처리전(開匣處理前) 미숙배종자(未熟胚種子)를 $30^{\circ}C$이상(以上)의 고온(高溫)에서 24시간(時間), 10일이상(日以上)의 침지처리(浸漬處理)는 배(胚)의 생장(生長) 및 발아율(發芽率)을 현저(顯著)히 지연(遲延)시켰다. 4. 미숙배종자(未熟胚種子)의 배생장(胚生長)은 $10^{\circ}C$에서 거의 정지상태(停止狀態)이나 $20^{\circ}C$에서 50일간(日間) 치상후(置床後) 계속(繼續) $10^{\circ}C$에서 50일간(日間) 치상(置床)할 경우(境遇) 생장(生長)이 현저(顯著)히 촉진(促進)되어 $20^{\circ}C$에서만 100일간(日間) 개갑(開匣)시킨 종자(種子)보다 배율(胚率)이 크게 증가(增加)되었다. 5. 개갑(開匣)이 완료(完了)된 종자(種子)의 계속적(繼續的)인 배생장(胚生長)은 $10^{\circ}C$에서 촉진(促進)되었으나 발아율(發芽率)은 $5^{\circ}C$에서 100일간(日間) 저온처리(低溫處理)한 종자(種子)에서 높았고 발아소요일수(發芽所要日數)도 짧았다. 6. 개갑(開匣)된 종차(種子)의 저온처리직전(低溫處理直前) 혹은 과정중(過程中) 침지(浸漬) 또는 20일간(日間) $20^{\circ}C$로의 저온상승처리(溫度上昇處理)는 저온처리중(低溫處理中)의 배생장(胚生長) 및 발아(發芽)를 현저(顯著)히 저해(沮害)하였다. 7. 저온처리(低溫處理)를 완료(完了)한 종자(種子)는 $10^{\circ}C$에서 최적(最高)의 발아율(發芽率)을 보였고 발아최적토양수분(發芽最適土壤水分)의 추정치(推定値)는 62.5%였으며 높은 산소요구도(酸素要求度)를 가지고 있었다. 8. 마쇄(磨碎)된 인삼종자(人蔘種子)의 증류수추출물(蒸溜水抽出物)만이 참깨, 조 및 콩의 발아억제효과(發芽抑制效果)를 뚜렷이 나타내었으며 개갑종자(開匣種子)보다는 미개갑종자추출물(未開匣種子抽出物)의 억제효과(抑制效果)가 더 컸고, 추출온도(抽出溫度)에 따른 억제효과(抑制效果)의 차이(差異)는 인정(認定)되지 않았다. 9. 인삼(人蔘)의 장육(醬肉), 잎, 뿌리 및 건조(乾操)한 뿌리의 물추출물(抽出物)도 참깨 및 인삼(人蔘)의 유묘생육(幼苗生育)을 현저(顯著)히 억제(抑制)하는 효과(效果)를 나타내었다. 10. 인삼(人蔘)의 종자(種子), 잎 및 뿌리의 증류수추출물(蒸溜水抽出物)은 진균(眞菌)의 발아(發芽) 및 균사생장(菌絲生長)을 크게 억제(抑制)하였으나 세균(細菌)의 증식(增殖)에는 영향(影響)을 미치지 않았다. 11. 인삼종자(

말초성 전정질환 환자에서의 회전반응 검사와 냉온자극 검사의 비교

원준연,이광선,윤태현,김윤경 대한이비인후과학회 1998 대한이비인후과학회지 두경부외과학 Vol.41 No.1

해가림자재에 따른 인삼의 엽록소 형광 반응 및 광합성 변화

원준연,이충열,오동주,김성만 한국약용작물학회 2008 한국약용작물학회지 Vol.16 No.6

This study was conducted to investigate the influence of shading material on the chlorophyll fluorescence, photosynthesis, transpiration, stomatal conductance and its any correlations in Panax ginseng C.A.Meyer. Fo was higher in polyethylene shade net than in silver-coated shading plate, but this treatment caused a lower Fm in comparison with silver-coated shading plate. Also, Fv/Fm and PhiPS2 showed higher in silver-coated shading plate than in polyethylene shade net. The relationship between net photosynthetic rate and transpiration, stomatal conductance were increased as the PAR (Photosynthetic active radiation) was increased and reached maximum at the 200-400 μmol/m2/s of PAR in all of leaves, and the higher in silver-coated shading plate than in polyethylene shade net. A linear equation was obtained between net photosynthetic rate and transpiration, net photosynthetic rate and stomatal conductance. SPAD was higher in silver-coated shading plate than in polyethylene shade net.

중소기업 정책자금의 지원방식 만족도에 관한 연구

원준연 한국무역학회 2015 한국무역학회 학술대회 Vol.2015 No.5

혈액투석 환자에서 이온화 칼슘의 직접 및 간접 측정의 비교

원준연,김성록 대한신장학회 1998 Kidney Research and Clinical Practice Vol.17 No.6

It is important to maintain normal calcium concentration especially ionized calcium concentration in chronic renal failure patients on hemodialysis. The direct measurement of ionized calcium is less commonly used due to a lack of automated equipment as well as the cost of laboratory equipment. Numerous formulas for adjusted total calcium and calculated ionized calcium are used in clinical practice. We examined the relationship between direct measured ionized calcium and total calcium, corrected total calcium, calculated ionized calcium(formula of Nordin et al) in 53 chronic renal failure patients on hemodialysis. The results were as follows; 1) In predialysis group, plasma total and ionized calcium levels were 2.36±0.26, 1.04±0.21mmol/L respectively, and higher than normal controls. The correlations between plasma ionized calcium and total calcium, calculated ionized calcium, corrected total calcium were r=0.72(P=0.0001), r=0.81(P=0.0001), r=0.65(P=0.0001) respectively. The plasma ionized calcium level was t correlated with the level of albumin, pH, phosphate, parathyroid hormone. 2) The plasma total and ionized calcium levels were significantly increased with hemodialysis and values were 2.49±0.14mmol, 1.14±0.14mmol/L respectively. The correlation between ionized and total calcium was r=0.41(P=0.0021). These results suggested that the calculated ionized calcium(formula of Nordin et al) and total calcium can be used to predict the plasma ionized calcium level in chronic renal patients on hemodialysis.

Age Differences in Pet Sensitization by Pet Ownership

원준연,권재우,홍승노,이우현 대한이비인후과학회 2021 Clinical and Experimental Otorhinolaryngology Vol.14 No.2

Objectives. The association between pet sensitization and pet ownership remains unclear. Therefore, we aimed to elucidate the association between pet sensitization and pet ownership by age. Methods. We retrospectively reviewed 2,883 patients who visited our allergy clinic for nasal symptoms from January 2003 to December 2014, of whom 1,957 patients with data on skin-prick tests and questionnaire responses were included and divided into adults (age >19 years) and children (age ≤19 years). The association between pet sensitization and pet ownership was evaluated in both groups. Results. Among children, dog and cat sensitization showed no associations with dog and cat ownership, respectively. However, among adults, dog sensitization was significantly associated with dog ownership (odds ratio [OR], 3.283; P<0.001), and cat sensitization with cat ownership (OR, 13.732; P<0.001). After adjustment for age, sex, familial history of allergy, sinusitis, diabetes mellitus, other pet ownership, and non-pet sensitization, significant associations remained between dog sensitization and dog ownership (adjusted OR [aOR], 3.881; P<0.001), and between cat sensitization and cat ownership (aOR, 10.804; P<0.001) among adults. Dog ownership did not show any association with allergic rhinitis, asthma, or atopic dermatitis, whereas atopic dermatitis had a significant association with cat ownership in adults (aOR, 4.840; P<0.001). Conclusion. Pet ownership in adulthood increased the risk of pet sensitization. However, pet ownership was not associated with the prevalence of atopic disorders, regardless of age, except for atopic dermatitis and cat ownership in adults.

현장 타설용 콘크리트의 시공품질관리를 위한 연구 ( A Study on The Construction Quality Management of Concrete on Building site Placement )

원준연,이종균,김성식,임남기,정상진 대한건축학회 2001 大韓建築學會論文集 : 構造系 Vol.17 No.1

동절기 단열갱폼으로 인한 경제성 효과 및 투입 원가 분석 연구

원준연,이영도,남경용,Won, Joon-Yuen,Lee, Young-Do,Nam, Kyung-Yong 한국건축시공학회 2018 한국건축시공학회지 Vol.18 No.4

This paper verifies the superiority of warming work in winter by applying the insulation gang-form to the apartment housing site and analyzes the economic feasibility of the application. According to the experimental results, the actual cost of warming work was about 52 million won less than planned, and 160 million won less than the existing average.(Note - The cost of gang form material increased from 260 million won to 310 million won after the change) As a result, the construction cost could be reduced by about 110 million won. As the costs of warming work can change depending on the number of floors, the building number, and the area of each site, it is deemed necessary to conduct a thorough review in advance at the site where the cost of warming work is to be applied. 본 논문은 단열갱폼의 공동주택현장 적용을 통해 단열갱폼 동절기 보양우수성을 확인하고, 현장 적용에 따른 경제적 타당성을 분석하고 있다. 실험결과에 따르면 실제 보양투입 비용은 사전계획대비 약 5천 2백만 원을 절감하였으며, 기존 일반갱폼대비 약 1억 6천만 원을 절감하였다.(참고 - 변경전 갱폼 자재비는 2억 6천만 원에서 변경 후 3억 1천만 원으로 약 5천만 원 증가함) 결과적으로 단열갱폼으로 변경하여 약 1.1억 원 가량의 공사비를 절감할 수 있었다. 현장마다 층수, 동수, 면적이 달라짐에 따라 보양공사비가 변경될 수 있으니 단열갱폼 적용 예정인 현장에서는 사전에 충분한 검토가 이루어져야 할 것으로 판단된다.

녹색쌀 생산을 위한 벼 품종들의 특성 비교

원준연,조진웅 한국작물학회 2015 한국작물학회지 Vol.60 No.4

This research analyzed the characteristics of varieties of rice such as a harvest time and yield, and to select a proper variety to product green rice according to a conventional planting culture and a late planting culture. The most proper harvest time of rice, in general, was 15 to 25 days after heading to product the green rice. Sinsunchal among glutinous rice varieties and Chilbo among nonglutinous rice varieties showed the most amount harvest at 25 days after heading, which was a limit harvest time for the whole green rice production in overall rice varieties. The amount of green rice according to transplanting times, the May 30 th transplanting was more than the June 20 th . The yield of green rice harvested at 15~25 days after heading was varied according to varieties. Proper glutinous varieties for green rice production were Dongjinchal, Sangdongchal, Boseokchal, and Sinsunchal, in order of listed, which were transplanted at the May 30 th . Meanwhile, Boseokchal, Backokchal, and Sinsunchal, in order of listed, were proper varieties for green rice production, which were transplanted at the June 20 th . In nonglutinous rice, Samkwang, Nunbora, and Chilbo, which were transplanted at the May 30 th , were proper varieties for green rice production. Hwanggeomnodeul, Hopum, and Chilbo, which were transplanted at the June 20 th , were proper varieties for green rice production. 녹색쌀 생산을 위한 벼 품종들의 적합 품종선발과 수확시기 및 수량 등을 알기 위하여 적기 및 만식재배에 의한 특성을 살펴본 결과 녹색쌀을 생산하기 위한 벼 품종들의 적정수확시기는 일반적으로 출수 후 15~25일 경이 가장 적당하였으며 벼 품종별 녹색통쌀의 한계 수확시기인 출수 후 25 일경 수량은 찰벼 품종으로는 신선찰벼가 가장 많았으며 메벼 품종은 칠보벼가 가장 많았다. 이앙시기별 녹색쌀 수량은 일반적으로 5월30일 이앙이 6월 20일 이앙보다 많았으며 수확시기인 출수 후 15~25일 수량은 품종에 따라 다양하였다. 녹색쌀에 적합한 벼 품종은 5월30일 이앙재배는 찰벼로는 동진찰벼, 생동찰벼, 보석찰벼, 신선찰벼 순으로 적합하였고 6월 20일 이앙재배는 보석찰벼, 백옥찰벼, 신선찰벼 순으로 적합하였다. 메벼는 5월 30일 이앙재배는 삼광벼, 눈보라, 칠보벼가 적합하고 6월 20일 이앙재배일 경우 황금노들, 호품벼, 칠보벼가 적합하였다.

Olfactory and Taste Dysfunction in Patients with Asymptomatic and Mildly Symptomatic COVID-19 in Korea

원준연,허윤,김태석,김충효,이우현 대한비과학회 2021 Journal of rhinology Vol.28 No.1

Background and Objectives: The association between chemosensory dysfunction (CSD) and coronavirus disease 2019 (COVID-19) remains unclear. The aim of the present study was to determine the incidence of olfactory and taste dysfunction in asymptomatic and mildly symptomatic patients with COVID-19 and to evaluate the symptoms associated with CSD in patients with COVID-19. Materials and Method: On March 9, 2020, 309 patients with asymptomatic or mildly symptomatic COVID-19 confirmed by real-time polymerase chain reaction (RT-PCR) were admitted to the No. 7 Community Treatment Center in Korea. An internetbased survey about symptomatology was administered to these patients, with responses obtained from 244 (79.0%). Subjects who completed the survey were enrolled in this study and were categorized into either a CSD group or a normal chemosensory group based on the presence or absence of CSD, respectively. Results: General symptoms, including fever, myalgia, and chills, were most common (29.1%), followed by upper respiratory tract infection (URI) symptoms (20.9%), CSD (20.5%), and nasal symptoms (13.5%). In patients with CSD (n=50), 10 (4.1%) reported no other symptoms. After adjustment for age, sex, past medical history, and other symptoms, general symptoms [odds ratio (OR), 3.63; confidence interval (CI), 1.70-7.76] and nasal symptoms (OR, 7.00; CI, 2.61-18.80) were significantly associated with CSD. Conclusion: The incidence of CSD was relatively high (20.5%) in asymptomatic and mildly symptomatic patients with COVID-19. General symptoms were independent risk factors of CSD, suggesting a sensorineural mechanism for the observed olfactory and taste dysfunction. Background and Objectives: The association between chemosensory dysfunction (CSD) and coronavirus disease 2019 (COVID-19) remains unclear. The aim of the present study was to determine the incidence of olfactory and taste dysfunction in asymptomatic and mildly symptomatic patients with COVID-19 and to evaluate the symptoms associated with CSD in patients with COVID-19.Materials and Method: On March 9, 2020, 309 patients with asymptomatic or mildly symptomatic COVID-19 confirmed by real-time polymerase chain reaction (RT-PCR) were admitted to the No. 7 Community Treatment Center in Korea. An internet-based survey about symptomatology was administered to these patients, with responses obtained from 244 (79.0%). Subjects who completed the survey were enrolled in this study and were categorized into either a CSD group or a normal chemosensory group based on the presence or absence of CSD, respectively.Results: General symptoms, including fever, myalgia, and chills, were most common (29.1%), followed by upper respiratory tract infection (URI) symptoms (20.9%), CSD (20.5%), and nasal symptoms (13.5%). In patients with CSD (n=50), 10 (4.1%) reported no other symptoms. After adjustment for age, sex, past medical history, and other symptoms, general symptoms [odds ratio (OR), 3.63; confidence interval (CI), 1.70-7.76] and nasal symptoms (OR, 7.00; CI, 2.61-18.80) were significantly associated with CSD.Conclusion: The incidence of CSD was relatively high (20.5%) in asymptomatic and mildly symptomatic patients with COVID-19. General symptoms were independent risk factors of CSD, suggesting a sensorineural mechanism for the observed olfactory and taste dysfunction.