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Characterization of elastin-like polypeptides (ELPs) and their applications as biomaterials
원종인 한국공업화학회 2020 한국공업화학회 연구논문 초록집 Vol.2020 No.-
Repetitive polypeptides are emerging as a new class of biomaterials due to their unique physical and biological properties. These repetitive peptide-based biomaterials can be applied to the design of new polymeric biomaterials with improved properties. As one of the repetitive polypeptides, elastin-like polypeptides (ELPs) are thermally responsive and can undergo a reversible phase transition. Due to their abilities to self-assemble and thermo sensitivity, ELPs have been applied to many research fields. In this study, an ELP expression system in E. coli was constructed and the expression level of ELPs according to the ELP length was explored in a cell-free system and in vivo system. Based on these results, I demonstrated that the expressed ELPs were applied to the various biological fields such as drug delivery system, biosensor for the detection of heavy metal contamination, a scaffold material in tissue engineering, and a useful tool for DNA analysis.
Recent Advances in DNA Sequencing by End-Labeled Free-Solution Electrophoresis (ELFSE)
원종인 한국생물공학회 2006 Biotechnology and Bioprocess Engineering Vol.11 No.3
End-Labeled Free-Solution Electrophoresis (ELFSE) is a new technique that is a promising bioconjugate method for DNA sequencing (or separation) and genotyping by both capillary and microfluidic device electrophoresis. Because ELFSE enables high-resolution electrophoretic separation in aqueous buffer alone (i.e., without a polymer matrix), it eliminates the need to load viscous polymer networks into electrophoresis microchannels. To achieve microchannel DNA separations with high performance, ELFSE requires monodisperse perturbing entities (i.e., drag-tags), which create a large amount of frictional drag when pulled behind DNA during free-solution electrophoresis, and which have other properties suitable for microchannel electrophoresis. In this article, the theoretical concepts of ELFSE and the required characteristics of the drag-tag molecules for the ultimate performance of ELFSE are reviewed. Additionally, the merits and limitations of current drag-tags are also discussed in the context of recent experimental data of ELFSE separation (or sequencing).
원종인 숙명여자대학교 2003 숙명語文論集 Vol.- No.5
Confucian ethics is dominant through the Chosun Dynasty and it is a fundamental principle of that period. In that society the rulling classes had prohibited strictly the remarriage of a woman by fidelity ideology which was an idea of Confucianism. But the social order was collapsed severly in late Chosun Dynasty. Dendong Omi whom we have analized in this article, didn't have a mind to follow the fidelity ideology. So she chose to start another marrige. She lost her husband four times and remarry three times. During flower pancake game she met a young widow who was in anguish whether she will marry or not. When Dendong Omi saw her situation, she told her own tough story. Finally the young widow escaped from the conflict about her marriage. The auther have presented repeatdly the lesson through Dendong Orni's pitiful life. The lesson is like this if one woman rernary , she must be hit by misfortune. So Dendong Omi Flower Pancake Song was written not for personal life but for establish ing Confucian ideology which had controlled the members in the society. The writer have inserted the life of Dendong Omi to the Dendong Omi flower Pancake Song that is abundant in lyricism. This work enlarged the formal patency and flexiblity of a boudoir poem. Traditional boudoir poim only spoke out moral lessons but the writer of Dendong Omi Flower Pancake Song put a story. the boudoir poem have been developed into high dimension form. It is the significance of Korean literature history.