1.2-Dichlorobenzene을 분해하는 Pseudomonas sp. DCB3의 분리 및 특성

서승교, 우철주, 이창호 한국환경보건학회 1997 한국환경보건학회지 Vol.23 No.4

Four bacterial strains able to degrade dichlorobenzene as the sole source of carbon and energy were isolated from soil by selective enrichment culture, and among them, one isolation was the best in the cell growth and identified as Pseudomonas sp. DCB3 by its morphology and physiological properties. Cell growth dramatically increased in a minimal medium containing 500ppm of dichlorobenzene was not detected any more at 72 hours after cultivation. The optimal temperature and initial pH for the growth of the isolated strain were 30$\circ$C and 7.0, respectively. Cell growth was increased by supplementing $(NH_2)_2CO$ and 50 ppm yeast extract as additional nutrients. Therefore, it was suggested that Pseudomonas sp. DCB3 could be effectively used for the biological treatment of wastewater containing dichlorobenzene.

환경 및 아세트 알데히드 제조 공장 폐수의 처리에 이용되는 Microbacterium Laevaniformans 가 생성하는 Isocitrate Lyase 의 정제 및 특성

서승교,우철주,전진곤,정기택 한국미생물생명공학회 1992 한국미생물생명공학회 학술발표초록집 Vol.1992 No.1

Posters ; 효소 및 생리활성 물질 ; Candida sp . L - 16 이 생산하는 Xylose Reductase 의 분리 정제 및 그 특성

이종수,우철주,서종교,정기택 한국미생물생명공학회 1991 한국미생물생명공학회 학술발표초록집 Vol.1991 No.1

버섯 폐상퇴비의 이화학성과 미생물 조사

주길재,우철주,이인구 慶北大學校農業科學技術硏究所 2002 慶北大農學誌 Vol.20 No.-

본 연구는 팽이버섯의 병재배용 폐배지를 이용하여 페상퇴비를 제조하고 토비의 이화학성 및 미생물을 분석한 내용이다. 폐상퇴비의 유기물 함량은 43.29%, 유기물함량/질소비는 27.00%, 전질소함량은 1.60%, 수분함량은 46.48%, 염농도는 0.64%, P_2O_5는 1.32%, K_2O 1.18%로 나타났다. 퇴비내 존재하는 미생물의 수는 1.6×10^10(cfu/g)으로 나타났으며, 이들 미생물을 동정한 결과 대부분이 Bacillus sp.으로 나타났고 주로 B. lentimobus, B. coagulans, B. brevis, Clostridium thermocellum, Escherichia coli 등이 존재하였고, 방선균류는 주로 Streptomyces thermovulgaris, S. thermovulgaris 등 Streptomyce네. 가 주종이며, Micropolyspora faeni도 존재하였다. 곰팡이류는 Aspergillus sp. 나 Penicillum sp. 등이 존재하는 것으로 확인되었다. This study was conducted to investigate physicochemical and microbiological properties on waste composts of mushroom. The waste compost of mushroom consisted of 43.29% organic matter(O.M.), 27.0 O.M./Nitrogen, 1.60% total nitrogen, 46.48% water content, 0.64% salt content, 1.32% P_2O_5, 1.18% K_2O and dry base. The microorganisms in the waste compost of mushroom were counted 1.6 x 10^10 cfu/g. The main population of aerobic bacteria were Bacillus, B. coagulans, B. brevis, Clostridium thermocellum, Esherichia coli, Streptomyces thermovulgaris, S. thermofuscus, Micropolyspora faeni, Aspergillus sp. and Penicillum sp..

D-xylose 발효효모의 분리 및 성질

이종수,우철주,송형익,정기택 한국미생물학회 1990 미생물학회지 Vol.28 No.4

D-xylose로부터 직접 ethanol을 생산할 목적으로 D-xylose를 유일한 탄소원과 에너지원으로 이용하는 효모들을 토양, 톱밥, 썩은 나무들로부터 분리하였다. 이 균주들 가운데서 ethanol 생산력이 가장 우수한 균주는 썩은 나무로부터 분리한 Candida sp. L-16으로 동정되었다. 이균주를 이용한 ethanol 발효의 최적 조건은 진탕속도 60rpm, 배양온도 28C, 초기 pH 4.5 및 D-xylose 농도 5%이었다. 알코올 생산은 배양 4일째에 최대치에 도달했고 이때의 ethanol 농도는 2.4%(v/v)로서 이론적인 ethanol 수율은 총당에 대해 74.4%이었다. In order to ferment D-xylose directly to ethanol, Yeasts capable of utilizing D-xylose as a sole carbon source and energy source were isolated from soil, sawdust and rotten woods. Among them, the yeast strain, which showed the best ability to produce ethanol, was identified as Candida sp. L-16 isolated from rotten woods. The optimal conditions for production of ethanol were 60rpm of agitation speed, 28j.deg.C of temperature, 4.5 of initial pH and 5% of D-xylose concentration. Ethanol production was reached to maximum state for 4 days culture. Under these optimal conditions, the maximum ethanol concentration and theoretical ethanol yield were 2.4%(v/v) and 74.4% of theoretical value, respectively.

난분해성 염소계화합물의 생물학적 처리

이창호,우철주,서승교 한국위생과학회 1997 한국위생과학회지 Vol.3 No.1

A bacterium SWR103 has been selected from various samples of industrial waste water and soil. Based an the morphological and physiological characteristics, the isolated SWR103 was identificated as Pseudomonas sp. and named as Pseudomonas sp. SWR103. The optimal temperature and initial pH for the growth of the isolated strain were 30℃ and 7.0, respectively. When 1,3-dichlorobenzene was added to the minimal media as a sole source of carbon and energy, the concentration of optimum for cell growth was 500ppm. When 500ppm 1,3-dichlorobenzene was given in hte minimal media, Pseudomonas sp. SWR103 completely unitilize it within 84hr. Cell growth was increased by supplementing 50ppm yeast extract as additional nutrients. Therefore, it was suggested that Pseudomonas sp. SWR103 could be effectively used for the biological treatment of waste water containing 1,3-dichlorobenzene.

Killer 효모 Saccharomyces cerevisiae B15-1의 에탄올 발효특성

이창호,우철주,이종수,정기택,박희동 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.3

정치배양에 의한 Saccharomyces cerevisiae B15-1의 에탄올 발효에 미치는 영향 인자와 초기 기질농도에 따른 발효 특성을 연구, 검토하여 에탄올 생성 최적 조건하에서 발효액중의 생성된 에탄올 농도, 균체 농도 및 당 농도와의 관계를 규명하고자 하였다. 에탄올 생성의 최적 조건은 배양 온도 30℃, 초기 pH 5.0이었으며, 에탄올 비생성 속도는 초기 기질농도가 150 g/l일 때 1.203 g-EtOH/g-cell·hr로서 가장 높게 나타났다. 에탄올 발효능은 기질농도가 150 g/l 이하에서는 거의 일정하였으나 200 g/l에서는 급격히 감소하였다. 최종 균체량과 최종 에탄올 생성량은 초기 기질의 농도가 증가함에 따라 증가하는 현상을 보였으나 그 증가율은 기질의 농도가 높을 수록 다소 감소하는 경향을 나타내었다. 초기 기질농도가 150 g/l 이하에서는 대부분이 발효에 의해 에탄올로 전환되었으나 200 g/l의 당을 사용한 경우에는 균체의 증식과 발효가 완료된 후에도 약 10.7 g/l의 기질이 에탄올로 전환되지 못하고 배지에 남아 있었다. 초기 기질 농도를 매개변수로 생성된 에탄올과 에탄올 수율을 조사한 결과 에탄올 수율은 초기 당농도가 증가할수록 급격히 감소하였으며, 초기 당농도가 일정한 경우 에탄올 생성량이 소량일 때는 에탄올의 생성량이 증가할수록 에탄올의 수율은 급격히 증가하다가 점차 완만한 증가현상을 보였으며 에탄올 생성량이 60 g/l 이상에서는 거의 일정하였다. Characteristics of ethanol fermentation were investigated during the stationary culture of a killer yeast, Saccharomyces cerevisiae B15-1. Specific ethanol production rate reached the maximum level, 1.203 g-EtOH/g-cell·hr, at 150 g/l of the initial glucose concentration. No big differences were obtained in ethanol fermentability based on the initial sugar concentration below 150 g/l. When 200 g/l of sugar was used, fermentability dropped significantly. Although the final cell mass and the amount of ethanol produced were increased, their increase rates were declined according to the increase of initial sugar concentration. It was found that most of the sugar used below 150 g/l of concentration could be changed to ethanol. However, when 200 g/l of sugar was used, some of them remained in the media even after increase of cell mass and fermentation stopped. The ethanol yield was decreased when initial sugar concentration was high, and were increased when the amount of ethanol produced was increased and finally reached the plateau over 60 g/l of ethanol concentration.

Candida sp . L-16 이 생산하는 Xylitol Dehydrogena SE 의 분리 정제 및 특성

이종수,서종교,우철주,정기택 한국미생물생명공학회 1992 한국미생물생명공학회 학술발표초록집 Vol.1992 No.-

동치미로부터 항돌연변이 물질을 생산하는 유산균의 분리 및 특성

주길재,이창호,우철주 한국생명과학회 2001 생명과학회지 Vol.11 No.5

Various lactic acid bacteria were isolated from Korean Dongchimi (whole radish Kimichi with added water) in order to study their antimutagenic activity. Ames test using Salmonella enterica serovar typhimurium TA98 and TA100 showed the strain DLAB19 to have the highest antimutagenic activity among the 300 isolated strains against MNNG(N-methyl-N-nitro-N-nitrosoguanidine), NPD (4-nitro-O-phenylenediamine), 4-NQO(4-nitroquinoline-1-oxide) and AFB$_{1}$(aflatoxin B$_{1}$). The strain was identified as Leuconostoc mesenteroides subsp. cremoris according to the Bergeys Mannual Systematic Bscteriology based on its morphological, cultural, physiological characteristics and biological system Antimutagenic activity of Leu. mesenteroides subsp. cremoris DLAB19 was found in the culture supernatant suggesting the bacterium secretes, the antimutagenic substance in the media. The antimutagenic activity of Leu. mesenteroides subsp. cremoris DLAB19 was reconfirmed by the spore-rec assay using spores of Bacillus subtilis H17 (Rec$^{+}$) and M45 (Rec$^{[-10]}$ ).).

Killer 효모 융합주 FWKS 260 이 분비하는 Killer Toxin 의 정제

정기택,방광웅,우철주,정용진,김재근,송형익 한국미생물학회 1992 미생물학회지 Vol.30 No.3

Killer toxin from killer yeast fusant FWKS 260 developed by protoplast fusion between the wild killer yeast and alcohol-fermenting yeast was purified by ammonium sulfate fractionation. Amicon PM I0 concentration. Sephadex G-200 and Scphadcx G-75 column chromatography. The purified killer toxin showed a single band by SIX-polyacvlamide gel electrophoresis. The protein part of killer toxin was active site. which was found by treating the proteolytic enzyme such as pronase E and pepsin to killer toxin. The killer toxin was stable at pH 2.0-5.0 and 20$^{\circ}$C. but inactivated with increasing temperature. The molecular weight was determined to be approximately 13.000 according to the results obtained from the SDS-polyacrylamide gel electrophoresis. It was confirmed that the purified killer toxin is glycoprotein by showing a red single band after st'tining with Schiffs reagent.