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오세량(Sei Ryang Oh),정근영(Keun Young Jung),이형규(Hyeong Kyu Lee) 한국응용생명화학회 1996 Applied Biological Chemistry (Appl Biol Chem) Vol.39 No.2
To screen inhibitors on complement system from natural resources, micro-screening method was established by using hemolytic complement assay. Complement fixation reaction was carried out in the microplate system. For standard hemolysis (50% hemolysis) of the classical pathway (CP), hemolysin and complement serum were diluted to 1/75∼1/100 and 1/80∼1/120, respectively, when sheep erythrocytes were 5.0×10^8 cells/㎖. In case of the alternative pathway (AP), complement serum was diluted to 1/5 and EGTA and Mg^(2+) were added 4 mM, 4∼8 mM, respectively, when rabbit erythrocytes were 4.0×10^8 cells/㎖. Dimethyl sulfoxide was used for the assay of non-aquous soluble compounds or extracts and its final concentration was not more than 1%. Three phenylpropanoids showed anticomplementary activities in proportion to the concentration for both pathways and rosmarinic acid exihibited the highest inhibitory activities: 5.4±3.6%(0.063 mM)∼95.8±0.2%(0.5 mM) and 35.1±0.9%(0.063 mM)∼95.6±1.1%(1 mM) on the CP and the AP, respectively.
방선균 분리주 No . 2077 이 생산하는 살충성 물질 SR 2077 의 구조 동정
오세량(Sei Ryang Oh),이형규(Hyeong Kyu Lee),최수근(Soo Keun Choi),김정일(Jeong Il Kim) 한국응용생명화학회 1994 Applied Biological Chemistry (Appl Biol Chem) Vol.37 No.4
During the screening of insecticidal compounds from soil microorganisms, SR 2077 was isolated from the metabolites of Actinomycetes isolate No. 2077 and identified as albocyctine by UV and NMR data analyses.
Streptomyces sp . no . 46 이 생산하는 살충성 물질의 구조 동정
오세량(Sei Ryang Oh),이형규(Hyeong Kyu Lee),구본탁(Bon Tak Koo),최수근(Soo Keun Choi),박상구(Sang Gu Park),신병식(Byung Sik Shin),박승환(Seung Hwan Park),김정일(Jeong Il Kim) 한국응용생명화학회 1994 Applied Biological Chemistry (Appl Biol Chem) Vol.37 No.2
In searching for new insecticidal compounds from soil microorganisms, strains of streptomyces species showed insecticidal activities on Musca domestica and Bombyx mori were selected. Compounds Ⅰ-Ⅳ, which were isolated from the metabolites of no. 46 strain, were identified as piericidin C₁, C₂, C₃ and D₁, respectively by UV and NMR data analyses.
감국(Chrysanthemi Flos)의 새로운 알킬알콜배당체 성분에 관한 연구
정근영,오세량,김천석,김정희,이형규,Jung, Keun-Young,Oh, Sei-Ryang,Kim, Chun-Suk,Kim, Jung-Hee,Lee, Hyeong-Kyu 한국생약학회 1996 생약학회지 Vol.27 No.1
In the course of phytochemical studies of Chrysanthermi Flos(Chrysanthemum indicum L., Compositae), two compounds were isolated by repeated column chromatography. Compound 1 is identified as adenosine on the basis of spectroscopic means and comparison with an authentic standard. Compound 2 is determined to be a new alkyl alcohol glycoside, 1-octen-3-ol $3-O-{\beta}-D-xylopyranosyl(1{\rightarrow} 6)-{\beta}-D-glucopyranoside$ on the spectroscopic evidence. Compounds 1 and 2 are reported for the first time from this plant.
UPLC-QTOF-MS분석를 이용한 국내산 더덕 주산지의 표지물질 선정
안영민 ( Young Min An ),장현재 ( Hyun-jae Jang ),김두영 ( Doo-young Kim ),백남인 ( Nam-in Baek ),오세량 ( Sei-ryang Oh ),이대영 ( Dae Young Lee ),류형원 ( Hyung Won Ryu ) 한국응용생명화학회 2021 Journal of Applied Biological Chemistry (J. Appl. Vol.64 No.3
더덕(Codonopsis lanceolata)은 주로 한국, 중국 등 동아시아 지역에 재배되고 있으며, 더덕의 뿌리는 기침, 기관지염, 천식, 결핵, 소화 불량의 증상을 치료하기 위한 기능성 식품 및 전통 의학으로 사용되어져 왔다. 보고된 바에 의하면 phenylpropanoids, polyacetylenes, saponins, flavonoids와 같은 다양한 식물 천연물 성분들이 항비만, 항염, 항암, 항산화, 항미생물 활성과 같은 약리학적 작용에 관여한다고 보고되어 있다. MS기반 대사체학 분석을 이용한 주산지의 마커 성분을 선정하는 것은 다른 지역에서 재배된 약용 식물의 안전성뿐만 아니라 화학적 조성과 생물학적 효능의 변화와도 관련이 있기 때문에 부작용 없이 더덕의 유익한 효과만을 보장하는데 중요하다. 본 연구에서는 국내산 더덕의 주산지 특성을 구별하기 위해 UPLC-QTOF-MS를 기반으로 하는 대사체 프로파일링과 다변량 통계분석 기법인 PCA 분석을 수행하여 판별모델을 확립하였다. 그 결과 인제(강원도), 횡성(강원도), 무주(전라북도)의 3개 그룹이 PCA와 loading plot 분석결과 tangshenoside I, lancemaside A, lancemaside G는 더덕 주산지를 구별하기 위한 잠재적 대사체 마커들로 제안하였다. Codonopsis lanceolata (Deoduk) was grown in East Asia, including Korea, China, Japan, and Russia, and the roots of C. lanceolata have been used as functional foods and traditional medicine to treat symptoms of cough, bronchitis, asthma, tuberculosis, and dyspepsia. The phytochemicals of C. lanceolata have been reported such as phenylpropanoids, polyacetylenes, saponins, and flavonoids that are involved in pharmacological effects such as anti-obesity, anti-inflammation, anti-tumor, antioxidant, and anti-microbial activities. Selecting marker substances of the main producing area by MS-based metabolomics analysis is important to ensure the beneficial effect of C. lanceolata without side-effects because differences in cultivated areas of plants were related not only to the safety of medicinal plants but also to changes in chemical composition and biological efficacy. In our present study, ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry combined with multivariate statistical analysis was applied to recognize the main producing area of C. lanceolata in South Korea. As a result of Principal Component Analysis and loading plot analysis of three groups, Inje (Kangwon-do), Hoengseong (Kangwon-do), and Muju (Jeonlabuk-do), several secondary metabolites of C. lanceolata including tangshenoside I, lancemaside A, and lancemaside G, were suggested as potential marker substances to distinguish the place of main producing area of C. lanceolata.
반하(Pinellia ternata)에서의 [6]-gingerol 함량과 멜라닌 저해 활성에 영향을 미치는 원산지 판별 마커로의 활용
류형원 ( Hyung Won Ryu ),안주현 ( Ju Hyeon An ),원효준 ( Hyo Jun Won ),서수경 ( Soo-kyung Seo ),김두영 ( Doo-young Kim ),구창섭 ( Chang-sub Ku ),오세량 ( Sei-ryang Oh ) 한국응용생명화학회(구 한국농화학회) 2016 Journal of Applied Biological Chemistry (J. Appl. Vol.59 No.4
반하(Pinellia ternata Breitenbach)는 천남성과(Araceae)의 약용식물로 동아시아 일대가 원산지이며, 유럽과 북아메리카 일부지역에도 널리 분포하고 있는 식물이다. 반하의 괴경(tuber)은 약재로 사용되어 구토, 염증, 외상을 치료하는 목적으로 사용되고 있다. 보고된 약리학적 작용으로는 항경련, 항종양, 살충, 세포독성 등이 있는 것으로 알려져 있다. 반하는 약리학적으로 유용한 약용식물로 잘 알려져 있음에도 불구하고, 원산지를 판별할 수 있는 신뢰성이 있고 표준화된 방법이 없는 실정이다. 이를 위한 한국산과 중국산 반하를 판별할 수 있는 유의적인 화합물을 탐색하기 위해 UPLC-PDA와 QTof-MS에 기반을 둔 대사체 표지법을 이용하였다. 한국산으로부터 원산지 판별 화합물로 예상되는 화합물을 반복적인 역상 칼럼크로마토그래피에 기반을 둔 활성유도 분획법을 통해 분리하였다. 그리고, NMR과 MS를 포함한 물리화학적, 분광학적 정보의 결과를 토대로 [6]- gingerol이라는 화합물의 구조를 동정하였다. [6]-gingerol은 원산지를 판별할 수 있는 능력과 melanin 생합성 저해 활성을 지닌 기능성 화장품 소재로서 가치가 있다고 판단되어짐에 따라 유효성분 원산지 판별 화합물로 Fingerprint법에 의해 선정되었다. 더욱이, 한국산과 중국산 반하 유래 [6]-gingerol의 함량 비교를 위해, 유효성이 검증된 분석법을 이용하여 이에 대한 검량곡선을 작성하여 그 함량을 비교하였다. 이것은 한국산 반하판별을 위한 화합물 선정과 성공적인 유효성 검증을 다룬 최초의 보고이다. Pinellia ternata Breitenbach, the natural medicinal plant of the Araceae family, is a perennial plant originated from the East Asia, but also widely distributed in Europe and North America. Its tuber is used as traditional medicine for treatment of various diseases such as vomiting, inflammation, and traumatic injury. Pharmacological studies revealed that P. ternata possesses anticonvulsant, anti-tumor, insecticidal, and cytotoxic activities. Despite being well-known as the useful medicinal plant, there is no reliable, standardized method for origin discrimination. Ultra performance liquid chromatography-photodiode array detector and quadrupole time of flight-mass spectrometry based metaboliteprofiling was applied to explore significant metabolite for origin discrimination between Korean and Chinese P. ternata. One compound was isolated from Korean P. ternata using repeated ODS column chromatography by bioactivity guided fractionation, and determined as [6]-gingerol according to the results of spectroscopic data including nuclear magnetic resonance and MS. This compound was selected as cosmeceutical biomarker by fingerprints, and it was associated to melanin inhibitory effect determining its origin authenticity. Furthermore, the calibration curve of biomarker was prepared using validated method for the comparison of content between Korean and Chinese P. ternata. This is the report to address the selection and successful validation of the discriminant metabolite for confirmation of Korean P. ternata.