일부 지역 주민들의 스트레스 관련요인에 대한 연구

오경재,이정미,길상선,권근상 대한예방의학회 2003 예방의학회지 Vol.36 No.2

Hepa1c1c7 세포에서 카드뮴에 의한 세포사멸 신호전달체계에 관한 연구

오경재,염정호 한국독성학회 2001 Toxicological Research Vol.17 No.3

Cadmium is an ubiquitous toxic metal and chronic exposure to cadmium results in the accumulation of cadmium in the liver and kidneys. In contrast, acute exposure leads to damage mainly in the liver. Apoptosis induced by cadmium has been shown in many tissues in vivo and in cultured cells in vitro. However, the molecular mechanism of cadmium-induced apoptosis is not clear in hepatocyte. To investigate the induction of apoptosis in the hepatocyte, we used mouse hepatoma cell line, Hepe1c1c7 cells, and analysed the molecules that involved in cadmium-induced apoptosis. Cadmium induced the genomic DNA fragmentation, PARP cleavage, and activation of caspase-3 like protease. Caspase-9 cysteine protease was activated in a time-dependent manner, but caspase-8 cysteine protease was not significantly activated in cadmium-treated Hepa1c1c7 cells. Cadmium also induced mitochondrial dysfunction including cytochrome c release from mitochondria, change of mitochondrial membrane potential transition, and translocation of Bax protein into mitochondria. These results strongly indicated that the signal pathway of apoptotic death in cadmium-treated Hepa1c1c7 cells is modulated by caspase cascade via mitochondria.

초등학생의 구강보건 지식수준과 구강보건 행동에 관한 연구

오경재,김정숙 원광대학교 대학원 2009 論文集 Vol.42 No.-

Among chronic diseases in Korean adults, dental canes is third most common, following arthritis and hypertension. Elementary students use medical services frequently for cold, ophthalmic diseases and dental canes, and dental caries appears to be experienced by all age groups. In Korea, dental canes prevention programs are increasing, and through such programs, health teachers responsible for students' health need to manage oral health as one of their important tasks. Desirable oral health programs should develop teaching methods for oral health education and induce people's interest in oral health and voluntary oral health management habits, and by doing so, promote oral health knowledge and behavior and make oral health prevention and treatment activities more effective. The present study purposed to survey oral health knowledge level and oral health behavior among 12 year old children in their mixed dentition period who may experience caries of permanent teeth, and to provide basic materials necessary to set directions for oral health education in school. For this, we collected data from 240 fifth graders (12 year old children) at an elementary school in Iksan, Jollabuk do during the period from the 13^(th) to 20^(th) of May, 2008, and excluding 12 respondents who answered inappropriately, we analyzed data from 228 children and drew conclusions as follows. 1. As to oral health knowledge level, 95.6% of the students knew that 'chocolate, cookie and candy cause caries easily.' 2. As to oral health knowledge level according to general characteristics, there were significant differences in oral health knowledge level according to father's academic qualification and mother's academic qualification(p<.05). Oral health knowledge level was significantly different according to the condition of teeth, but in the results of posterior analysis, no notable difference was observed among the groups(p<.05). 3. As to oral health behavior related to toothbrush according to general characteristics, a significant difference was observed only according to gender(p<.05). 4. As to experience in preventive dental treatment according to general characteristics, a significant difference was observed only according to father's academic qualification(p<.05). 5. As to oral health knowledge level according to the installation and operation of oral health room in school, there was no significant difference in the necessity for the installation of oral health room, and there was a significant difference in the necessity for oral health teachers(p<.05). 6. As to the necessity for oral health teachers according to general characteristics, it was significantly different only according to gender and the condition of teeth(p<.05). According to gender, while 57.6% of male students replied 'Necessary,' 28.8% 'Don't know' and 13.6% 'Not necessary,' 71.8% of female students replied 'Necessary,' 14.6% 'Don't know' and 13.6% 'Not necessary.' This shows that both male and female students think that oral health teachers are necessary.

RAW 264.7 Cell에서 납에 의한 NO 생성의 조절에 미치는 Glutathione의 효과

오경재,권근상,윤욱희,신새론,Oh, Gyung-Jae,Kwon, Keun-Sang,Yoon, Wook-Hee,Shin, Sae-Ron 대한예방의학회 2002 Journal of Preventive Medicine and Public Health Vol.35 No.4

Balb/c 마우스의 복강내에 Abelson leukemia virus (A-MuLV)를 주입하여 발생시킨 대식세포주 RAW 264.7 세포의 배양조건에 납과 NAC및 BSO를 첨가하여 세포생존율과 NO 및 ATP 생성량의 변화를 관찰한 결과, 납을 처리한 기본배양조건에서 RAW 264.7 세포의 생존율은 각 농도에서 차이가 없었으며 NO의 생성량은 $0.5{\mu}M$의 납농도에서부터 용량 의존적으로 감소하였으나 ATP의 생성량은 각 농도군에서 차이가 없었다. NAC을 전처리하고 납을 처리한 배양조건에서의 NO 및 ATP의 생성량은 대조군과 차이가 없었다. BSO를 전처리하고 납을 처리한 배양조건에서의 NO의 생성량은 납만 처리했을 때와 달리 각각의 농도군에서 대조군과 차이가 있었다. ATP생성량은 역시 차이가 없었다. 이상의 결과에서 납의 농도가 증가함에 따라 ATP의 생성량은 변화가 없으면서 NO가 감소하는 것을 볼 때, 납에 의한 대식세포에서 NO생성의 억제기전은 수은 및 카드뮴 등과 같이 미토콘드리아에 영향을 미쳐 ATP생성이 억제됨으로 L-arginine-NO경로에서 ATP를 필요로 하는 iNOS가 작용을 못하여 NO 생성이 저하되는 기전과는 다른 기전이 있음을 보여준다. 또한 iNOS의 조효소인 세포내 GSH를 증가시키는 NAC을 전처리했을 때 NO의 생성량이 대조군 수준으로 회복되고 세포내 GSH를 감소시키는 BSO를 전처리했을 때는 오히려 NO의 생성량에 영향을 미치지 않는 납의 농도에서조차 NO 생성의 감소가 일어난 것으로 볼 때 GSH는 대식세포에서 NO생성을 저하시키는 납의 독성에 보호작용이 있음을 확인할 수 있었다. Objectives : To evaluate the elect of glutathione(GSH) on lead induced modulation of nitric oxide(NO) synthesis, and to examine how lead modulates NO production in macrophages. Methods : This study was observed in a culture of RAW 264.7 cells, which originated from a tumor in a Balb/c mouse that was induced by the Abelson murine leukemia virus. The compounds investigated were lead chloride, N-acetyl-cystein(NAC), and Buthionine Sulfoximine( BSO). Results : ATP synthesis in RAW 264.7 cells was unchanged by each lead concentration exposure in a dose dependent manner. The NO synthesis was decreased when exposed to lead($PbCl_2$) concentration $0.5{\mu}M$. The presence of $300{\mu}M$ NAC, used as a pretreatment in the culture medium, caused the recovery of the lead induced decrease in NO synthesis, but in the presence of $300{\mu}M$ BSO as a pretreatment, there was no recoverey. Pretreatment with NAC and BSO had no affect on ATP synthesis at any of the lead concentrations used. Conclusions : These results indicated that GSH has a protective effect toward lead toxicity, and suggested that the inhibition of NO production in macrophage due to lead toxicity may be related to cofactors of iNOS (inducible nitric oxide synthase)

고혈압, 당뇨병, 이상지질혈증 진단을 받은 성인의 직업별 심뇌혈관질환 인지도

오경재,이영훈 사단법인 대한보건협회 2018 대한보건연구 Vol.44 No.2

Objective: This study investigated the difference in the awareness of cardio-cerebrovascular disease (CCVD) according to occupation among Korean adults diagnosed with pre-existing diseases of CCVD including hypertension, diabetes mellitus, or dyslipidemia. Methods: Data from the 2013 Korea Community Health Survey was used. A total of 61,459 adults (26,237 males, 35,222 females) older than 40 years diagnosed with hypertension, diabetes mellitus, or dyslipidemia were included in the final analysis. Occupation was classified as managers/professionals (MP), clerks (CL), service/sales workers (SS), agricultural/forestry/fishery workers (AFF), mechanical/manual laborer (MM), and housewives/unemployed people (HU). Association between occupation and the awareness of CCVD was examined using the logistic regression analysis after adjusting for socio-demographics. Results: The awareness of CCVD was the highest in CL (48.3%) and the lowest in AFF (19.5%) among males, and the highest in MP (54.0%) and the lowest in AFF (11.4%) among females, respectively. In males, the fully-adjusted odds ratio (OR) for awareness of CCVD of AFF (OR, 0.84; 95% confidence intervals [CI], 0.74-0.95) and MM (OR, 0.85; 95% CI, 0.76-0.94) than MP were significantly lower. In females, the fully-adjusted OR for awareness of CCVD of AFF (OR, 0.56; 95% CI, 0.47-0.68), MM (OR, 0.59; 95% CI, 0.49-0.70), HU (OR, 0.68; 95% CI, 0.57-0.79), CL (OR, 0.71; 95% CI, 0.56-0.90), and SS (OR, 0.77; 95% CI, 0.65-0.92) than MP were significantly lower. Among the detailed pathways, there were gender-specific occupational differences of awareness of CCVD by internet, public advertising, medical clinics, and public health centers, individually. Conclusion: In Korean adults diagnosed with hypertension, diabetes mellitus, or dyslipidemia, significant disparities in the awareness of CCVD were identified by gender and occupation. To reduce the disparity of awareness of CCVD, public health efforts with an emphasis on AFF and MM in both genders will be needed. 연구목적: 본 연구는 고혈압, 당뇨병, 이상지질혈증이 있는 우리나라 성인에서 직업군에 따른 심뇌혈관질환 인지도의 차이가 있는지를 확인하고자 하였다. 연구방법: 2013년 지역사회건강조사에 참여한 대상자 중 고혈압, 당뇨병 또는 이상지질혈증의 진단을 받은 40세 이상 성인 61,459명(남자 26,237명, 여자 35,222명)을 최종 분석대상으로 하였다. 직업군은 전문행정관리직, 사무직, 판매서비스직, 농림어업, 기능단순노무직, 주부∙무직의 6개로 분류하였다. 직업과 심뇌혈관질환 인지도의 관련성은 로지스틱 회귀분석을 이용하여 인구사회학적 특성(연령, 거주지역, 교육수준, 혼인상태, 월평균 가구소득, 국민기초생활수급)을 보정한 후 평가하였다. 연구결과: 심뇌혈관질환 인지도는 남자에서는 사무직(48.3%)이 가장 높았고 농림어업(19.5%)이 가장 낮았으며, 여자에서는 전문행정관리직(54.0%)이 가장 높았고 농림어업(11.4%)이 가장 낮았다. 고혈압, 당뇨병, 또는 이상지질혈증 진단을 받은 남자에서는 전문행정관리직에 비해 농림어업(odds ratio [OR], 0.84; 95% confidence intervals [CI], 0.74-0.95)과 기능단순노무직(OR, 0.85; 95% CI, 0.76-0.94)의 심뇌혈관질환 인지 교차비가 유의하게 낮았다. 여자에서는 전문행정관리직에 비해 농림어업(OR, 0.56; 95% CI, 0.47-0.68), 기능단순노무직(OR, 0.59; 95% CI, 0.49-0.70), 주부∙무직(OR, 0.68; 95% CI, 0.57-0.79), 사무직(OR, 0.71; 95% CI, 0.56-0.90), 판매서비스직(OR, 0.77; 95% CI, 0.65-0.92)의 심뇌혈관질환 인지 교차비가 유의하게 낮았다. 심뇌혈관질환 인지의 각 세부경로 중 인터넷, 대중광고, 병의원, 보건기관을 통한 각각의 심뇌혈관질환 인지도는 성 및 직업군에 따라 유의한 차이가 있었다. 결론: 고혈압, 당뇨병, 이상지질혈증의 진단을 받은 우리나라 성인에서 직업군에 따른 심뇌혈관질환 인지도의 유의한 차이가 확인되었다. 심뇌혈관질환 인지도의 형평성을 개선하기 위해서는 특히 남녀 모두에서 상대적으로 인지도가 낮은 농림어업과 기능단순노무직에 특화된 공중보건중재가 필요하겠다.

2003 전북지역 전염병관리 실무과정(FMTP)의 교육요구도 평가

오경재,박재영 圓光大學校 醫科學硏究所 2005 圓光醫科學 Vol.20 No.2

폐경 후 여성의 거주 지역에 따른 여가시간신체활동과 관련요인

오경재 원광대학교 의과학연구소 2009 圓光醫科學 Vol.24 No.1

이 연구는 일개 도·농 통합 도시에서 무작위로 도심 및 농촌 각각 1개 마을 및 통 지역을 선택한 후 해당 지역에 거주하는 지역 주민 중 연구의 목적을 충분히 이해하고 동의한 50세 이상 여성 196명을 대상으로 실시하였다. 조사는 2007년 6월 1일부터 22일까지 3주에 걸쳐 이루어졌으며, 자료는 연구자로부터 교육을 받은 조사원들에 의해 직접면접 조사를 통해 수집하였다. 대상자들의 일반적인 특성에서 농촌지역 여성은 도시지역 여성보다 상대적으로 학력이 낮고 직업적으로는 1차 산업 종사자가 많이 분포하였다. 가구소득은 전반적으로 농촌지역이 도시지역보다 소득수준이 낮게 나타나고 있다. 폐경 후 경과기간은 도시여성보다 농촌여성에서 더 긴 것으로 나타났다. 한편 도시지역 여성과 농촌 여성의 규칙적인 운동실천율은 차이가 없었다. 그러나 여가시간신체활동지수는 농촌 여성보다 도시 지역 여성에서 높은 것으로 나타났다. 여가시간신체활동지수를 종속변수로 하는 다변량 분석 결과에서는 영향 요인은 농촌지역 여성에서는 가구소득이, 도시지역에서는 폐경 후 경과기간으로 나타났다. 이러한 결과는 농촌 여성에 있어서의 낮은 소득수준은 건강증진이나 유지를 위한 여가시간 신체활동을 저해하는 주요한 요인임을 제시하고 있으며 도시 여성들의 경우 심리적 박탈감과 내분비계의 급격한 기능 변화로 만성질환에의 이환 위험성 또한 크게 상승하는 폐경 초기의 신체활동에 관심을 가져야 하는 근거를 제시하고 있다. 결론적으로 만성질환 예방 및 건강증진을 위해 신체활동을 증가시키기 위해서는 거주 지역에 따른 여성들의 특성을 고려해야 하며 만성질환 예방 및 건강증진에 있어서는 운동 외에도 포괄적인 신체활동을 대표할 수 있는 관련 지표들을 이용해야 할 것으로 사료된다. Background : This study described the physical activity of postmenopausal women and examines the relationships among leisure-time physical activity(LTPA) behavior, background, and resides correlates of behavior between urban and rural area Methods : The study included 177 residents, who lived in a small city from June 1 to 22 2007. Respondents were interviewed by means of a structured questionnaire. Data analyses were conducted using x²-test, Monte Carlo test, and multiple logistic regression analysis. Results : This study showed that general characteristic factors in women who reside rural were significantly higher than women who reside downtown in the following categories: people older than 60 years, people who were engaged in the agriculture, and low income. Period after menopause was higher in women who reside rural than women who reside downtown, but no significant difference was found between residential districts in BMI, age at menarche, age at menopause, and duration of breast feeding. Leisure time activity index was significantly higher. than in women who reside downtown. Household income associated with leisure time activity in women who reside rural, while period after menopause associated with it in women who reside downtown. Conclusion : These results suggest that leisure time activity was found to be a practical index for degree of physical activity, and it should be considered intervention strategies for control chronic disease, especially in postmenopausal women who reside rural.

Indicators Related to Cardiopulmonary Resuscitation According to Occupation Among Family Members of Coronary Heart Disease Patients

오경재,이경숙,김경수,이영훈 전남대학교 의과학연구소 2020 전남의대학술지 Vol.56 No.3

This study aimed to evaluate differences in cardiopulmonary resuscitation (CPR)-related indicators among families of community-dwelling coronary heart disease (CHD) patients according to their occupation. A total of 6,867 family members living with CHD patients were selected for analysis from the 2016 Korea Community Health Survey. Respondents’ occupations were classified into managers and professionals (MP), clerks (CL), service and sales workers (SSW), agricultural/forestry/fishery workers (AFFW), mechanical and manual laborers (MML), and homemakers and unemployed (HU). The adjusted odds ratio (aOR) for awareness of CPR in the MP (3.82), SSW (1.73), and MML (1.29) groups were higher than that in HU (reference), while the CL (1.42) and AFFW (1.04) groups showed no significant difference compared to HU. The aORs for experience with CPR education and manikin-assisted CPR training were higher among the MP (4.00 and 3.94), CL (2.61 and 2.26), SSW (2.02 and 1.91), and MML (1.99 and 1.69) groups than in HU, and only AFFW (1.22 and 1.18) showed no difference from HU. Finally, the aOR for self-efficacy in CPR performance was significantly higher among the MP (3.17), CL (1.64), SSW (1.87), and MML (1.44) groups than in HU. However, there was no significant difference between AFFW (1.22) and HU in self-efficacy in CPR performance. To improve the survival rate of CHD patients through successful CPR at the pre-hospital stage during cardiac arrest, it is important to increase the ability of family members of CHD patients to perform CPR, especially among those in AFFW and HU occupations.

수은에 의한 EMT-6 세포의 $NO_2^-$ 및 ATP 생성

오경재,고대하,염정호,Oh, Gyung-Jae,Koh, Dai-Ha,Youm, Jung-Ho 대한예방의학회 1996 예방의학회지 Vol.29 No.3

수은이 세포성 면역반응에 미치는 효과와 그 기전을 밝히기 위해 cytokine-EMT-6 cell-NO 모형에 대조군에는 수은을 첨가하지 않았고 나머지 실험군에는 $0.05-0.8{\mu}M$의 mercury chloride를 처리하였다. 그리고 배양시간별, 수은농도별로 세포생존율, 세포성 면역의 지표인 NO를 nitrite로 간접정량하고 대사과정에 필수 에너지요소인 nitrite를 측정한 결과는 아래와 같이 요약된다. 1. EMT-6세포의 세포생존율은 대조군과 36시간째 $0.8{\mu}M(89.50%)$을 제외한 모든 수은첨가군에서 모두 90%이상의 값을 나타내었으며, 배양시간 및 첨가한 수은농도별 군간 차이는 없었고 모든 수은첨가군과에서 대조군에 비해 큰 차이가 없었다. 2. 수은에 노출된 EMT-6 세포의 nitrite 생성량 및 ATP생성량은 배양시간의 경과에 따라 전반적으로 증가하였으며 시간경과에 따른 nitrite 생성량과 ATP 생성량은 첨가한 수은농도의 변화에 따라 각 군간에 현저한 차이를 보였다. 그리고 시간경과의 영향을 보정한 상태에서도 첨가한 수은농도의 변화에 따라 각 군간 차이는 모두 현저하게 나타났다. 한편, Nitrite 생성량과 ATP 생성량은 둘 다 수은첨가군 모두에서 대조군의 값에 비해 현저하게 낮았고, 첨가된 수은농도와 nitrite 생성량 및 ATP 생성량 사이에는 높은 음의 상관관계가 있어 수은농도의 증가에 따라 용량-반응의 관계(dose-dependent)로 감소하는 경향을 보였다. 이상의 결과, 배양조건에 수은의 첨가로 인하여 nitrite 생성량과 ATP 생성량이 동시에 감소하는 바, 수은에 의한 세포성 면역의 저하는 면역세포의 NO 생성량의 감소에 의한 것으로 사료되며, 이는 수은이 세포내 에너지생산에 관련된 대사과정을 억제시키므로 나타나는 결과라고 판단된다. Effect or mercury chloride on the synthesis or $NO_2^-$ and ATP were observed in EMT-6 cells which were cultured with cytokines$(IL-1\alpha\;and\;IFN-\gamma)$ and various concentrations of mercury chloride from 0.05 to $0.8{\mu}M$. Viability of EMT-6 cells were observed above 90% in almost groups. There were not significant differences in the viability between mercury supplemented groups and control group. It suggests viability of EMT-6 cells were not influenced by these concentrations of mercury chloride. Results of the synthesis of nitrite showed significant time and group effect. There is a significant interaction effect between concentration of mercury chloride and culture time. The effect of various concentration of mercury chloride is not the same for all levels of culture time. There were significant differences in the synthesis of nitrite between mercury chloride supplemented groups and control group, and the synthesis of nitrite in EMT-6 cell by the supplement of mercury chloride was significantly decreased in a dose-dependent manner. Results of the synthesis of ATP showed a significant group effect, and the time main effect and the $Group{\times}Time$ interaction were also significant. There were significant differences in the synthesis of ATP between mercury chloride supplemented groups and control group, and the synthesis of ATP in EMT-6 cell by the supplement of mercury chloride was significantly decreased in a dose - dependent manner. These results suggest that the disorder of cell mediated immunity by mercury chloride could be related to the inhibition of nitric oxide synthesis which will be caused by the decreased synthesis of ATP.

Public Awareness of Stroke and Its Predicting Factors in Korea: a National Public Telephone Survey, 2012 and 2014

오경재,문지영,이유미,박형근,박기수,윤용운,강길원,김병권,서재희,이희영,이원경,이건세,김희숙,이영훈 대한의학회 2016 Journal of Korean medical science Vol.31 No.11

The aim of this study was to investigate time trends in the public awareness of stroke and its predicting factors. The target population was 9,600 community-dwelling adults, aged 19–79 years, in 16 metropolitan cities and provinces in Korea. The survey samples in 2012 and 2014 were selected separately (entirely different sets of subjects) using a proportionate quota sampling method. Information concerning knowledge of stroke and demographics was collected by trained telephone interviewers using random digit dialing. After excluding subjects with a non-response or refusal to answer any question, the analyses included 8,191 subjects in 2012 and 8,127 subjects in 2014. Respondents’ awareness of stroke warning signs (numbness or weakness, difficulty speaking or understanding speech, dizziness, visual impairment, and severe headache) was highest for difficulty speaking or understanding speech (80.9% in 2012 and 86.4% in 2014). There were significant increases in the proportion of respondents understanding the appropriate action (i.e., calling an ambulance) at the time of stroke occurrence (59.6% to 67.1%), and in the proportion aware of the general need for prompt treatment (86.7% to 89.8%). In multivariable logistic regression analysis, older age, higher education level, higher household income, current non-smoking, exposure to stroke-related public relations materials, and experience of stroke education were significantly associated with both high knowledge of stroke warning signs and awareness of the need for prompt treatment. Between 2012 and 2014, the public’s awareness of stroke increased significantly. More specialized interventions, including public relations materials and education, should focus on subgroups who have lower stroke knowledge.