Microfluidic Cell Culture Systems for Cellular Analysis

연주헌,박제균 한국바이오칩학회 2007 BioChip Journal Vol.1 No.1

Microfluidic systems have significant implications for the fields of cell biology and cell-based assay as they enable conventional assays to be conducted using an automated and high-throughput approach. Unlike conventional in vitro cell culture methods, microfluidics can provide small and complex structures mimicking the in vivo environment of cells. Recent research has shown that microfluidic cell culture systems convey more reliable results due to their ability to grow cells as biological systems do, and because they outperform those from conventional cell cultures and assay systems. According to a variety of cell types, different microfluidic platforms have been reported. The performance of microfluidic devices highly depends on the inner structure providing cells with the in vivo-like environment to cells. In this paper, the microfluidic cell culture systems developed are reviewed and categorized according to their cell type and assay development. Potential applications of current microfluidic cell culture systems are also addressed.

도움받기에서 도움주기로 가는 여정

연주헌 한국뇌과학연구원 2022 브레인 Vol.93 No.-

왜 캐나다로 포스닥을 가셨나요?

연주헌 한국생물공학회 2015 BT NEWS Vol.22 No.1

Microfluidic platform for angiogenesis and vascular fusion

연주헌(Ju Hun Yeon),호청평(Qing Ping Hu),전누리(Noo Li Jeon) 대한기계학회 2010 대한기계학회 춘추학술대회 Vol.2010 No.11

Angiogenesis and vascular fusion is a complex cellular process which has an essential role in tumor growth, tumor metastasis and other pathological processes. Various in vivo and in vitro models have been developed for studying angiogenesis and anastomosis. In vivo models, the observation of angiogenesis is technically difficult and observed effects are also difficult to quantify. For this reason, the compound screening for angiogenesis on in vivo models was limited and the development of angiogenesis research was restrained. Recently, several in vitro models have been attempted to recreate the complex sequence of events of angiogenesis with varying degrees of success. However, the formation of new blood vessels and blood vessel fusion were not attempted to study in vitro models because it was not proved the appropriate model for angiogenesis and vascular fusion. In this study, we developed a novel microfluidic platform using the appropriate micro-architecture and 3D gel for mimicking angiogenesis. This device demonstrates its capability to precise control the cellular environment, the direction of vessel growth and the distribution of new vessels. Microfluidic platform for angiogenesis and vascular fusion will improve the study about specific vascular fusion and will be beneficial to quantitative angiogenesis assay in further research.

Toxicity Monitoring of HepG2 Cultured Cell Chip

Ju Hun Yeon(연주헌),Je-Kyun Park(박제균) 한국생물공학회 2004 한국생물공학회 학술대회 Vol.2004 No.10

Chip-base Impedance Measurement of Animal Cells Cultured in Peptide Hydrogel

Ju Hun Yeon(연주헌),Ji Hyun Sin(신지현),Minseok S. Kim(김민석),Je-Kyun Park 한국생물공학회 2005 한국생물공학회 학술대회 Vol.2005 No.10

국학기공 프로그램이 여성노인의 심박변이도와 혈중 세로토닌 농도에 미치는 영향

심준영,유성모,신재한,강윤정,연주헌 한국노인복지학회 2018 노인복지연구 Vol.73 No.1

본 연구는 국학기공 수련이 여성노인의 심박변이도(HRV)와 혈중 세로토닌 농도에 미치는영향을 알아보는데 있다. 이를 위하여 여성노인 40명을 대상으로 실험집단 20명과 대조집단20명으로 구분하고 실험집단에 대해 10주간, 주 2회, 회당 60분씩 국학기공 프로그램을 실시하였다. 분석결과, 심박변이도는 SDNN Index, RMSSD, pNN50에서 실험집단의 평균값이 대조집단에 비해 유의하게 높게 나타났으며, 우울의 지표가 되는 세로토닌 농도에서도 실험집단의평균값이 유의하게 더 많은 증가를 보였다. 이러한 결과는 국학기공 수련이 여성노인의 스트레스에 대한 생리적 회복탄력성을 높이고, 우울감을 해소하여 생리적 건강상태를 개선하는데 도움을 줄 수 있음을 시사한다. This research investigates the effect of Kookhak-Qigong program on the heart rate variability (HRV) and serotonin level in blood for elderly women. We designed a training program that consists of 2 sessions of 60 minutes training per week for 10 weeks against 40 elderly women, randomly divided into the experimental group (20) and control group (20). After the 10 weeks of training, the SDNN Index, RMSSD, and pNN50 results showed that the average HRV of the experimental group was significantly higher than the control group. The concentration of Serotonin level as an indication of depression was significantly increased in the experimental group than the control group. These results show that Kookhak-Gigong program increases the biological resilience against stress in elderly women, alleviates depression, and helps in improving physiological health conditions.

[뇌교육 가이드] “뇌교육의 탄생과 비전”

장래혁,양현정,하나현,연주헌,최윤리,김재홍,김나옥,신채은,김진희,이윤성,신재한,신혜숙,이수경,권효숙,오창영,노형철,박종찬,김태균,강나리,황현정(사진),김민석(사진) 한국뇌과학연구원 2017 브레인 Vol.65 No.-

중년 직장 여성의 스트레스와 우울에 미치는 뇌교육 기반 기공 프로그램의 효과

오미경,신재한,강윤정,연주헌 사단법인 인문사회과학기술융합학회 2018 예술인문사회융합멀티미디어논문지 Vol.8 No.12

The purpose of this study is to develop a Brain Education based Kigong program to reduce stress and depression in middle – aged women and to verify the effectiveness of the program. We used the questionnaire scale to measure the degree of stress and the questionnaire scale to measure the degree of depression as a tool to measure the dependent variables and analyze the blood to confirm their biochemical changes. The subjects of this study were 40 female (40 experimental group, 20 control group) who were in the 40 ~ 60 age group of nutrition team at oo university in Jeonnam. Both the experimental group and the control group were given pre – and post - stress questionnaire and depression scale questionnaire and blood was collected. The experimental group performed the Brain Education based Kigong program for 12 weeks, twice a week (50 minutes), and the control group did not. Data were analyzed using independent sample t-test, paired t-test, and two-way ANOVA analysis. The results of the study showed that Brain Education based Kigong programs reduced the degree of depression in middle – aged women and did not show a significant decrease in stress level. Blood analysis showed that insulin, total cholesterol, and LDL cholesterol showed a higher rate of decrease in the experimental group compared to the control group, but no statistically significant difference was found. These results suggest that Brain Education based Kigong program may have a effectiveness on depression in middle – aged women, but the identification of biological indicators should be clarified in future studies. 본 연구는 중년 직장 여성의 스트레스와 우울을 감소시키기 위한 뇌교육 기반 기공프로그램을 개발하고 프로그램의 효과를 검증하는 것을 목적으로 한다. 종속변수를 측정하기 위한 도구로 스트레스의 정도를 측정하는 질문지 척도와 우울의 정도를 측정하는 질문지 척도를 사용하였으며, 이들의 생화학적 변화를 확인하고자 혈액을 분석하였다. 연구대상은 전남의 oo대학교 영양팀 40~60대 여성 40 명 (20명 실험집단, 20명 통제집단)을 대상으로 하였다. 실험집단과 통제집단 모두 사전과 사후에 스트레스 척도와 우울척도 설문을 실시하고 혈액을 채취하였다. 실험집단은 주 2회(50분) 12주간 뇌교육 기반 기공 프로그램을 실시하였으며, 통제집단은 하지 않았다. 자료 분석은 독립표본 t검증, 대응표본 t검증 및 two-way ANOVA분석이 사용되었다. 연구결과는 뇌교육 기반 기공 프로그램이 중년 직장여 성의 우울의 정도를 감소시키는 것으로 나타났으며, 스트레스 정도에서는 유의미한 감소가 나타나지 않았다. 혈액 분석 결과, Insulin, Total cholesterol, LDL cholesterol에서 통제집단에 비하여 실험집단 에서 감소율이 더 높게 나타났지만 통계적으로 유의한 차이가 나타나지 않았다. 이러한 결과는 뇌교육 기반 기공 프로그램이 중년여성의 우울정도를 감소시키는 데 영향을 미치는 것이라 할 수 있겠으나 생물학적인 지표의 확인은 앞으로의 연구에서 밝혀져야 할 것이다.

Monitoring the Differentiation and Migration Patterns of Neural Cells Derived from Human Embryonic Stem Cells Using a Microfluidic Culture System

이나연,박재우,김형준,연주헌,권지혜,고정재,오승훈,김현숙,김애리,한백수,이상철,전누리,송지환 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.6

Microfluidics can provide unique experimental tools to visualize the development of neural structures within a microscale device, which is followed by guidance of neurite growth in the axonal isolation compartment. We utilized microfluidics technology to monitor the differentiation and migration of neural cells derived from human embryonic stem cells (hESCs). We co-cultured hESCs with PA6 stromal cells, and isolated neural rosette-like structures, which subsequently formed neurospheres in suspension culture. Tuj1-positive neural cells, but not nestin- positive neural precursor cells (NPCs), were able to enter the microfluidics grooves (microchannels), suggesting that neural cell-migratory capacity was dependent upon neuronal differentiation stage. We also showed that bundles of axons formed and extended into the microchannels. Taken together, these results demonstrated that microfluidics technology can provide useful tools to study neurite outgrowth and axon guidance of neural cells, which are derived from human embryonic stem cells.