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나노물질의 세포독성 평가법으로 Colony Forming Efficiency Assay에 대한 검증연구
조은혜,이재우,박선영,김필제,최경희,엄익춘,Jo, Eunhye,Lee, Jaewoo,Park, Sun-Young,Kim, Pilje,Choi, Kyunghee,Eom, Igchun 한국환경보건학회 2015 한국환경보건학회지 Vol.41 No.1
Objectives: The cytotoxcities of Au, Ag, SWCNT, $SiO_2$, and ZnO nanomaterials were evaluated in order to assess their potential toxicological effects in in vitro cell models using colony forming efficiency (CFE) assay. Methods: The CFE assay of the test materials was carried out on Hep G2 cells. The size distribution of nanomaterials was studied by transmission electron microscopy (TEM). Changes in cell viability after treatment with a toxicant will result in a decreased number of colonies formed in comparison to solvent. Results: The TEM images show that all the particles except SWCNT and ZnO can be considered approximately spherical. The gold and $SiO_2$ nanoparticles show no response (no toxicity) in concentration response experiments. A statistically significant toxic effect was found in Hep G2 cells treated with Ag, SWCNT and ZnO nanomaterials. Conclusion: In this study, we considered CFE assay to be a promising test for screening studies for cytotoxicity with physicochemical analysis.
류현수(Hyeonsu Ryu),윤효정(Hyojung Yoon),엄익춘(Igchun Eom),박진현(Jinhyeon Park),김순신(Sunshin Kim),조만수( Mansu Cho),양원호(Wonho Yang) 한국환경보건학회 2018 한국환경보건학회지 Vol.44 No.2
Objectives: The purpose of this study was to provide basic data for air pollutant exposure modelling and understanding the contribution of respective microenvironments by assessing the time-activity patterns of Korean students according to variables such as grade, sex, weekday, and weekend. Methods: In this study, we compared the residential time of 521 (both weekday and weekend) lower elementary students, 1,735 (1,054 on weekdays, 681 on weekends) upper elementary students, 2,210 (1,294 on weekdays, 916 on weekends) middle school students, and 2,366 (1,387 on weekdays, 979 on weekends) high school students in different microenvironments according to grade, sex, weekday, and weekend. We used data from the 2014 Time-Use Survey by the Korean National Statistical Office for upper elementary students through high school students, and surveyed time-activity patterns of 521 lower elementary students aged 7-9 years. Each microenvironment was divided into indoor, outdoor, and transport. Indoor environments were divided into home, school, and other places. In addition, the results of previous studies were compared to this study. Results: Weekday time-activity patterns of Korean students indicated that lower elementary students spent 16.02±2.53 hr in the home and 5.37±2.32 hr in school. Upper elementary students spent 14.11±1.79 hr in the home and 6.27±1.37hr in school. Middle school students spent 12.83±2.22 hr in the home and 7.48±1.88 hr in school. High school students spent 10.65±2.86 hr in the home and 10.23±2.86 hr in school on weekdays. High school students spent the least amount of time in the home and the most time in school compared to other grades Conclusions: Students spent most of their time indoors, including in the home, school, and other indoors. On weekdays, as the grade increases, home residential times were decreased and school residential times were increased. Differences in time-activity patterns according to sex were not found for either weekdays or weekends. It is estimated that Korean students could be affected by school indoor air quality. High school students could be most affected by school indoor air quality since they spent the most time at school.
SWCNT 및 MWCNT의 기관내 점적주입 후 폐 계면활성제의 분석
이병우(Byeongwoo Lee),서정관(Jungkwan Seo),심일섭(Ilseob Shim),엄익춘(Igchun Eom),김필제(Plije Kim) 한국환경보건학회 2017 한국환경보건학회지 Vol.43 No.4
Objectives: Carbon nanotubes (CNTs) are next-generation industrial nanoparticles which possess excellent mechanical strength along with good thermal conductivity and electric properties. Given these characteristics, carbon nanotubes are being widely applied in various fields, including research and development. However, concerns have been raised over hazardous properties due to their similar fiber shape to asbestos. Recent studies have shown that CNTs pose potential hazards which may cause fibrosis and/or lung inflammation similarly to asbestos. Methods: After intratracheal instillation of SWCNTs and MWCNTs to rats, pulmonary surfactant (PS) of the SWCNTs and MWCNTs was measured and analyzed using bronchoalveolar lavage fluid collected from the lung. After a single intratracheal instillation of SWCNTs and MWCNTs, phospholipid predominantly showed a significant increase compared to the control group, while proteins exhibited a significant increase both three days and one week after instillation. Results: As a result of surface tension, MWCNTs showed a significant decrease three days after treatment compared to the control group. In the case of the total cell number three days after instillation, MWCNTs revealed a temporarily significant increase when compared to the control group. For PMN number, when compared to the control group, SWCNTs displayed a significant increase throughout the observation period, while MWCNTs showed a significant increase three days and three months after treatment. Conclusions: After exposure to CNTs, the total cell number and PNT number, which indicate inflammatory response, were significantly increased. Therefore, this study suggests fiber–shaped CNTs may have a harmful effect on the lungs.
생물시험법을 이용한 중금속 물질의 급성 및 피부독성 평가
조은혜 ( Eunhye Jo ),유선경 ( Sun Kyoung Yoo ),성화경 ( Hwa Kyung Sung ),김은정 ( Eunjeong Kim ),엄익춘 ( Igchun Eom ),김필제 ( Pilje Kim ) 한국환경분석학회 2017 환경분석과 독성보건 Vol.20 No.2
Cadmium chloride(CdCl<sub>2</sub>), copper(II) chloride(CuCl<sub>2</sub>), potassium dichromate(VI)(K<sub>2</sub>Cr<sub>2</sub>O<sub>7</sub>), zinc chloride (ZnCl<sub>2</sub>) and nickel(II) chloride(NiCl<sub>2</sub>) are found in a wide range of products. The purpose of this study was to determine the acute toxicity and skin irritancy of the five chemicals using in vitro systems, and compare the results with in vivo endpoints. In this study, BALB/c 3T3 cells were exposed to several concentrations of CdCl<sub>2</sub>, CuCl<sub>2</sub>, K<sub>2</sub>Cr<sub>2</sub>O<sub>7</sub> ZnCl<sub>2</sub>, and NiCl<sub>2</sub>,and the neutral red uptake (NRU) assay was used to analyze cell viability. In addition, the chemicals were applied to the EpiDerm human skin model (EPI-200) to determine which of them causes significant cytotoxicity. Compared with the UN GHS and EU risk phrases, the assay results exhibited a high prediction rate (100%) in the skin bioassay. In contrast, the acute toxicity bioassay results were more similar to the lethal dose, 50% obtained from rat experiments than GHS and R-phrases. In summation, these findings indicate that acute toxicity and skin irritancy bioassays can be utilized as an alternative to the use of animals in evaluating chemical toxicity.