http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
윤영진,김한준,이덕원,엄세욱,전흥재 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.67 No.-
Surface modified poly l-lactic acid (PLLA) samples with hydroxyapatite (HA), heparin and bone morphogenetic protein-2 (BMP-2) mediated by polydopamine (pDA) coating (PLLA/pDA/HA/Hep/BMP-2) were prepared, and their effects on the enhancements of bone formation and osseointegration were evaluated in vitro and in vivo as compared to PLLA, PLLA/pDA/HA, and PLLA/pDA/Hep/BMP-2. The changes in surface chemical compositions, morphologies and wettabilities were observed by X-ray photoelectron spectroscopy (XPS), field-emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM) and water contact angle measurements. Pre-coating of HA particles with pDA provided uniform and homogeneous anchoring of particles to PLLA surface. In addition, the strong ionic interaction between heparin and pDA led PLLA surface readily heparinized for loading of BMP-2. In vitro experiments revealed that the levels of alkaline phosphatase (ALP) activity, calcium deposition, and osteocalcin (OCN) gene expression were higher in MG-63 human osteosarcoma cell lines grown on PLLA/pDA/HA/Hep/BMP-2 than on control PLLA, PLLA/pDA/HA, and PLLA/pDA/Hep/BMP-2. In vivo studies using micro-computed tomography (micro-CT) also showed that PLLA/pDA/HA/Hep/BMP-2 screw exhibited greatest value of bone volume (BV) and bone volume/tissue volume (BV/TV) among samples. Histological evaluations with H&E and Von Kossa staining demonstrated that a combination of HA and BMP-2 contributed to the strong osseointegration.
유영범,현훈,윤선중,김소연,이덕원,엄세욱,홍성옥,양대혁 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.67 No.-
Wounds that heal with excessive scar formation result in poor functional and aesthetic outcomes. To address this, in our study, visible light cured glycol chitosan (GCH) hydrogels containing endothelial growth factor (EGF) and basic fibroblast growth factor (bFGF) were prepared (GCH-EGF, GCH-FGF and GCH-EGF/FGF) and evaluated their efficacies on the improvement of wound healing in vivo. In vitro release test showed that the growth factors were released in a sustained manner along with initial burst for 24 h. In vitro cell proliferation assay of L-929 mouse fibroblast cell line resulted in the superior ability of GCH-EGF/FGF on the rate. In vivo results demonstrated that the growth factor loaded GCHs further enhanced wound healing compared with GCH. In particular, GCH-EGF/EFG showed the most remarkable wound healing effect among the samples.
현훈,이성수,임원봉,조단비,정진석,조가영,김소연,이덕원,엄세욱,양대혁,전흥재 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.70 No.-
In this study, we prepared a beta-cyclodextrin (β-CD)-based carrier consisting of β-CD, polyethyleneglycol (PEG) and folic acid (FA) (CDPF) for improved doxorubicin (DOX) delivery to targeted breast cancerin vitro and in vivo. The morphology and size distribution were characterized by transmission electronmicroscopy (TEM) and dynamic light scattering (DLS) measurements, which revealed in particles rangingfrom 38 nm to 52 nm in size. DOX from CDPF was released in a sustained manner for 48 h. Cell viabilityanalysis showed the low toxicity of free DOX, whereas CDPF-DOX remarkably exhibited reduced viabilityafter incubation for 7 days. In vivo animal test showed that intravenous injection of CDPF-DOXcontributes to decreased tumor volume, along with no systemic toxicity and cardiotoxicity. The resultssuggested that CDPF can maximize the efficacy of DOX delivery; therefore can be used as a goodcandidate for developing optimal drug delivery systems.