알긴산나트륨과 키토산을 이용한 Niacinamide의 경피 흡수특성

엄미선 ( Mi Sun Um ),문기섭 ( Ki Seop Moon ),채지혜 ( Ji Hae Chai ),정광보 ( Kwang Bo Chung ),이한섭 ( Han Seob Lee ),정병옥 ( Byung Ok Jung ),정석진 ( Suk Jin Chung ),김경호 ( Kyung Ho Kim ),원성권 ( Sung Kwon Won ),김동권 ( Dong 한국키틴키토산학회 2014 한국키틴키토산학회지 Vol.19 No.1

Drug delivery technologies are protected formulation technologies that modify drug release profile, absorption, distribution and elimination for the benefit of improving product efficacy and safety, as well as user convenience and compliance. The most commonly used transdermal system is the skin patch and gel using various types of technologies. Compared with other method of dosage, it is possible to use for a long term. It is also possible to stop the drug dosage if the drug dosage lead to side effect. In this study, Polysaccharide, such as chitosan and sodium alginate were selected as base materials for Percutaneous Absorption. Also, these natural polymers were characterized in terms of enhancers, niacinamide contents. Among these polysaccharide, the permeation rate of chitosan matrix was fastest in Niacin amide such as hydrophilic material in vitro. We used glycerin, PEG 400 and 800 as enhancers. Therefore, transdermal absorption of niacinamide could be improved by changing vehicle composition or using penetration enhancers. Especially it would be anticipated that the high permeation efficacy could be obtained using vehicle that has enhancing effect for itself and by adding enhancers to it.

제주 자생 우슬(Achyranthes japonica Nakai)의 항산화, 항염증과 항주름 평가

엄미선(Mi-Sun Um) 한국응용과학기술학회 (구.한국유화학회) 2021 한국응용과학기술학회지 Vol.38 No.5

본 연구는 화장품 소재로서 우슬(Achyranthes japonica Nakai)의 가능성을 확인하기 위한 것이다. 우슬 추출물이 가지고 있는 항산화, 항염증, 항주름 효과를 측정하였다. 각각의 식물 재료는 70% 에탄올을 이용하여 우슬 뿌리(Achyranthes japonica Nakai roots, AJNR)와 우슬 줄기 (Achyranthes japonica Nakai stalks, AJNS)로부터 추출하였다. RAW 264.7 세포를 배양하여 추출물의 Nitric oxide assay를 진행하였고, 섬유아세포 CCD-986sk를 배양하여 추출물의 MMP-1 assay, Type I procollagen synthesis assay를 실시하였다. 이 연구의 결과, 항산화 활성은 우슬 뿌리와 우슬 줄기 모두 우수하였고, 뿌리는 함염증 효과가 월등하게 우수하였으며 줄기는 뿌리에 비해 MMP-1 저해 활성과 Type I procollagen 합성 효과가 조금 더 높았다. 이 연구의 결과, 우슬 뿌리와 우슬 줄기의 항산화 활성은 유사한 수준으로 우수하였고, 뿌리의 함염 활성은 줄기보다 월등하게 높았다. MMP-1 저해 활성과 Type I procollagen 합성 효과는 대체적으로 우수하였는데 줄기가 뿌리에 비해 가 조금 더 뛰어났다. 그러므로 우슬은 항산화, 항염증, 항주름의 활성을 갖는 기능성 화장품 소재로서 활용이 가능할 것으로 판단된다. The aim of this study was to assure the possiblility of Achyranthes japonica Nakai for a cosmetic material. For this we examined the Anti-oxidation, Anti-inflammation, Anti-wrinkle effects of Achyranthes japonica Nakai extract. Each material is extracted from Achyranthes japonica Nakai roots(AJNR) and Achyranthes japonica Nakai stalks(AJNS) using 70% ethanol. We carried out Nitric oxide assay of the extract with cultured RAW 264.7 macrophage cells, And we conducted MMP-1 assay, Type I procollagen synthesis assay of the extract with cultured CCD-986sk fibroblast cells. After we measured ABTS+ assay and SOD assay of AJNR and AJNS, we knew that effect of anti-oxidant increased as their concentration get higher. The result of this study was that the antioxidant activity of AJNR and AJNS was similarly excellent and the anti-inflammatory activity of AJNR was significantly higher than AJNS. The MMP-1 inhibitory activity and Type I procollagen synthesis of AJNR and AJNS was generally excellent, AJNS had slightly better than AJNR. Therefore I thought it has the chance to play an imfortant role as a functional cosmetic material for anti-oxidant, anti-inflammation and wrinkle reduction effects.

Bacillus subtilis natto 균주로 발효한 병아리콩 낫토를 이용한 mask pack의 피부 개선 효과

엄미선(Mi Sun Um),류희욱(Hee Wook Ryu) 한국유화학회 2018 한국응용과학기술학회지 Vol.35 No.1

In order to utilize health food chick bean as a raw material for cosmetics, the skin improvement effect of a mask pack made of chickpea Natto fermented with Bacillus subtilis Natto strain was studied. Powder obtained by lyophilization of chickpea Natto was applied to a facial mask pack as a paste formulation. This Natto pack was applied to subjects in their 50s and 60s to investigate their effect on skin improvement. As the number of treatments of Natto pack increased, the skin was improved and the improvement effect was remarkable after 4 treatments. After four times treatments, moisture content and sebaceous secretion increased by 8.4 ± 3.6%p and 4.0 ± 2.3%p, respectively. Skin pores and wrinkles were also decreased by 1.8 ± 0.3%p and 1.8 ± 0.9%p, respectively. Skin pigmentation decreased by 1.3 ± 0.2%p and skin tone also increased from 55.2% to 55.9%. These results indicate that Natto product obtained by fermenting chickpea has the effect of improving skin such as moisturizing, pore, wrinkle, pigmentation, sebum secretion and skin tone, and can be used as a raw material for various functional cosmetics. 건강 식품인 병아리콩을 화장품의 원료로 활용하기 위하여 Bacillus subtilis Natto 균주로 병아리콩을 발효한 낫토를 마스크 팩에 적용하여 피부개선 효과를 연구하였다. 병아리콩 낫토를 동결건조하여 얻은 분말을 paste 제형으로 얼굴 마사지 팩에 적용하였다. 50~60대 여성을 대상으로 낫토 팩의 피부개선 효과를 조사하였다. 낫토 팩의 처치 횟수가 증가함에 따라 피부가 개선되며 4회 처치 후 개선효과가 두드러졌다. 4회 처치 후 수분 함량과 피지 분비량이 각각 8.4±3.6%p와 4.0±2.3%p 증가하였고, 피부 모공과 주름도 각각 1.8±0.3%p와 1.8±0.9%p 감소하였다. 피부 색소침착은 1.3±0.2%p 감소되었고, 피부 톤은 55.2%에서 55.9%로 0.7±0.2%p 밝아졌다. 병아리콩을 발효하여 얻은 낫토 발효물이 보습, 모공, 주름, 색소침착, 피지분비, 피부톤 등의 피부개선 효과가 있으며, 다양한 기능성 화장품의 원료로 활용 가능성을 확인하였다.

피부개선에 미치는 마스크 제형의 영향

엄미선 ( Mi Sun Um ),류희욱 ( Hee Wook Ryu ) 한국미용학회 2015 한국미용학회지 Vol.21 No.5

This study is the test for the analysis of the changes in the skin conditions before and after using gel type, sheet type, and carboxy type facial masks, to see the effects of their different formulations. Gel mask, sheet mask, and carboxy mask that contain ingredients such as 1% of hyaluronic acid, 2% of niacin amide, 0.04% of adenosine were each applied by 7 test subjects and by a total of 21. Skin analysis was conducted to objectively evaluate the changes in the moisture, pores, pigmentation, wrinkles and sebum before and after applying beauty masks for 30 minutes once a week in the test of 8 weeks` duration. As a result, all the three facial masks showed that the moisture increases in proportion to the number of uses(F=91.081, p<0.001) and there was no statistical significant difference according to formulations between them. The wrinkles measurement was shown to decrease since the 5th use(F=5.024, p<0.01) and there was no statistical significant difference according to their formulations. There was no statistically significant difference according to their formulations and the number of uses in the improvements of pigmentation. However, the effect of gel mask has gradually increased compared to before using it. The pores and the sebum didn`t show statistically significant difference depending on the type and the number of uses but the effect of gel and carboxy mask has increased step by step compared to before using them.

Phthalate 3종에 대한 경피투과 특성 연구

정덕채,윤철훈,엄미선,황현석,백정훈,최진호,Jung, Duck-Chae,Yoon, Cheol-Hun,Um, Mi-Sun,Hwang, Hyun-Suk,Baek, Jung-Hun,Choi, Jin-Ho 한국환경보건학회 2013 한국환경보건학회지 Vol.39 No.4

Objectives: Phthalates are used in a large variety of products including as coatings of pharmaceutical tablets, film formers, stabilizers, dispersants, emulsifying agents, and suspending agents. They have been the subject of great public concern in recent years. The extensive uses of this material have attracted attention and issues regarding its safety have been raised. Methods: In this study, three types of phthalate skin permeation were studied using matrixes such as ointments, creams and lotions in vitro. The absorption of phthalate diesters [Dimethyl phthalate (DMP), Di-n-propyl phthalate (DPP) and Di-n-pentyl phthalate (DNPP)] using film former has been measured in vitro through rat skin. Epidermal membranes were set up in Franz diffusion cells and their permeability to PBS measured in order to establish the integrity of the skin before the phthalates were applied to the epidermal surface. Results: Absorption rates for each phthalate ester were determined and permeability assessment made to quantify any irreversible alterations in barrier function due to contact with the esters. Types of phthalate in vitro experimental results quickly appeared in the following order DMP > DPP ${\geq}$ DNPP. Conclusions: In the experimental results, lotion> cream> ointment, and the permeation rate of lotion with a great amount of moisture was the fastest. Skin permeation rate is generally influenced by the chemical characteristics of a given chemical, such as molecular weight and lipophilicity. As the esters became more lipophilic and less hydrophilic, the rate of absorption decreased.