암환자의 가족지지와 희망에 관한 연구

어은영 병원간호사회 1998 임상간호연구 Vol.3 No.2

For cancer patients, family support and perceived hope by himself is an important factor to fight against cancer and to enhance his own quality of life. This study was conducted to propose the basic data for nursing care that support well beings and quality of life for cancer patients through evaluating family support, perceived hope and their relationship. The author executed questionnaire survey on 147 patients who hospitalized, and recognized about his own diagnosis, in one university hospital at Pusan from December 1 to December 31, 1996. The data was analyzed by PC SAS(Window ver 6.11) with level of significance α=0.05. The subjects had no difference in gender and age distribution, and whose diagnosis was cancer of stomach(29.9%), hepatobiliary(14.3%), breast(12.9%), rectal(11.6%), lung(10.9%), etc. Around half of them(42.9%) was diagnosed within three months, and 54.4% took drug therapy, nursing care was mainly provided by spouses(55.8%). The mean of the family support and perceived hope by five points scale was 3.93±0.82 and 3.48±0.62, respectively, and there showed positive correlation between the two scores(r=0.6338, p<0.01). For the family support, there showed significant difference by age, type of cancer and type of therapy, and for the perceived hope there showed significant difference by age, presence of pain, interval after diagnosis (p<0.05). That is, the mean of family support was lower in the age group of 50~59, in the case of hepatobiliary, pancreas and rectum, and in the case of drug and radiology combined therapy than the other classifications. The mean of perceived hope was lower in painful case, in the age group of 50~59, and in seven to twelve months interval after diagnosis than the other classifications(p<0.05). And, the mean of family support and perceived hope was higher in cancer of colon and stomach than the others by the type of cancer. In contrast that, there was no difference of family support and perceived hope by gender, marital status, religion, education level, occupation, socioeconomic status, number of family, main financial provider, main nursing provider, number of hospitalization, history of operation, severity of pain, etc. In conclusion, there showed positive correlation between family support and perceived hope, and it may be different according to age, type of cancer, pains, interval after diagnosis, therefore, for the supportive and efficient nursing care to cancer patients, it might be helpful to set up the nursing care that can encourage family support.

비전자기록물 이관업무 개선방안 연구 - 부산지역 기초자치단체를 중심으로 -

어은영,조지영 한국기록관리학회 2012 한국기록관리학회지 Vol.12 No.3

As records managers are assigned in accordance with act on public records management, the record management has settled down a bit, and also much effort is put to perform the record management in accordance with law. The record management includes all the works like production, classification, organization, transfer, collection, evaluation, disuse, preservation, opening to the public and application. Among them, the record transfer is an important work that performs the initial stage in which the main agent of management is changed from administration department to record center. Thus this study suggested the improvement measures for non-electronic record transfer after examining the current transfer state of 16 local governments in Busan region and also problems occurring in the process of transfer through interviews with institutional records managers. 공공기록물 관리에 관한 법률에 따른 기록물관리전문요원의 배치가 이루어지면서 기록물관리는 어느 정도 자리를 잡아가고 있으며, 법률에 따른 기록물관리를 이루기 위해 많은 노력을 기울이고 있다. 기록물관리는 기록물의 생산 · 분류 · 정리 · 이관 · 수집 · 평가 · 폐기 · 보존 · 공개 · 활용 및 이에 부수되는 제반업무를 말한다. 이중에서도 기록물 이관은 해당 기록물이 처리과에서 기록관으로 관리주체가 변경되는 최초 단계를 수행하는 중요한 업무라고 할 수 있다. 이에 따라 본 연구에서는 부산지역 16개 기초자치단체의 이관현황을 살펴보고, 기관의 기록물관리전문요원들의 인터뷰를 통해 이관진행과정에서 발생하는 문제점을 살펴보고 비전자기록물 이관업무 개선방안을 제시하였다.

퇴원시간 단축을 위한 개선활동

어은영 한국의료QA학회 2009 가을학술대회 Vol.2009 No.3

Antitumor Effects of Adenovirus-RUNX3 on Human Lung Cancer Cells

박미영,어은영,임효정,박종선,조영재,윤호일,이재호,배석철,이춘택 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-

Introduction: Runt-domain transcription factor (RUNX3) has an important role in gene regulation in development. In several cancers, such as gastric cancer, RUNX3 showed tumor suppressive function. Frequent inactivation of RUNX3 by methylation also was found in lung cancer, especially adenocarcinoma. Interestingly, lung adenocarcinoma is observed in RUNX3 heterozygous mice. Recently, inactivation of RUNX3 draws an attention as a potential marker of tumor stem cell. Materials and Methods: We constructed a recombinant adenovirus expressing RUNX3 (ad-RUNX3) by homologous recombination and an adenovirus expressing RUNX3 dominant negative form (ad-RUNX3 DNF) as a nonfunctioning control adenovirus. We tested the function of these adenoviruses in several lung cancer cell lines. Results: Transduction of ad-RUNX3 in lung cancer cell lines showed the production of RUNX3 protein and the expression of RUNX3 was enhanced by vorinostat, histone deacetylase inhibitor which could increase the transduction and transcription of adenoviral transgene. Transduction of ad-RUNX3 showed moderate antitumor effect in lung cancer cell lines. However, combined treatment of ad-RUNX3 with vorinostat showed strong synergistic antitumor effect with enhanced induction of apoptosis. Conclusion: Restoration of RUNX3 via adenoviral vector demonstrated antitumor effect. Further study on ad-RUNX3 will elucidate the role of RUNX3 in lung tumorigenesis.

암 환자의 가족지지와 희망에 관한 연구

전진호,어은영,손병철 인제대학교 1999 仁濟醫學 Vol.20 No.1

The Effects of Sleep Fragmentation on Gene Therapy in Murine Melanoma Model

김세중,어은영,박미영,강혜란,임가진,김유림,이연주,박종선,조영재,윤호일,이재호,이춘택 대한결핵 및 호흡기학회 2015 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.120 No.-

Introduction: Sleep fragmentation (SF) is a highly prevalent condition and a hallmark of sleep apnea. Recent studies suggested that SF may have associations with cancer growth and progression. However, it is not known that SF affects cancer treatment. The aim of this study was to investigate the effects of gene therapy in murine melanoma model according to the SF. Methods: Four-week-old C57BL/6 mice weighting 25g were used in this study. Melanoma cell (B16-F10) was injected subcutaneously. SF was conducted by a mouse forced exercise walking wheel system (Lafayette instruments, IN, USA). The wheel was activated for 30 sec and remained stopped for 90 sec from 7 AM to 7 PM. The light was on at 7 AM and off at 7 PM with ad libitum accessing to food and water. For gene therapy, interleukin (IL)-12 expressing oncolytic adenovirus was injected to the tumor. Results: Without gene therapy, tumor volume was not significantly different between SF group (n=22, 7.3 [4.9] cm3) and home cage (HC) group (n=20, 8.9 [4.1] cm3) (P=0.256). Serum IL-6 and TGF-β1 were significantly higher in SF group (79.5 [57.2] pg/ml, 62307.0 [26954.3] pg/ml) than in HC group (34.3 [8.3] pg/ml, 34737.2 [14528.2]) (P=0.044, P=0.039, respectively). After gene therapy, tumor volume showed smaller tendency in SF group (n=6, 3.6 [2.6] cm3) than HC group (n=4, 6.4 [4.6]cm3) (P=0.26). Conclusion: This study suggested that SF might improve the effectiveness of the gene therapy for melanoma. SF enhanced the productions of cytokines, which could help the oncolytic adenovirus more easily delivered to melanoma cells.

F-71 : Free Paper Presentation ; Reversal of Resistance to EGFR Tyrosine Kinase Inhibitor by EGFR T790M Specific siRNA in Non-Small Cell Lung Cancer

박미영,어은영,이연주,박종선,조영재,윤호일,이재호,이춘택 대한결핵 및 호흡기학회 2013 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.116 No.-

Mutations in EGFR tyrosine kinase domain become driver oncogenic mutation in lung adenicarcinoma. EGFR TKI induces dramatic response in lung adenocarcinoma with sensitive mutations. Unfortunately, this dramatic response can not last long and resistance to EGFR TKI emerges and induces treatment failure. More than 50% of resistant mutations are EGFR T790M mutation. In this study, we investigated the role of siRNA specific to EGFR T790M. We designed three sequences (siRNA1, 2, 3) specific to EGFR T790M. Lung cancer cells were used: A549, NCI H460 (EGFR; wild type), NCI H1975 (EGFR L858R+T790M), PC9 (EGFR small deletion in exon 19), PC9-G (EGFR small deletion in exon 19+T790M). We investigated the effect of three siRNAs on suppression of EGFR T790M and reversal of resistance to gefitinib. Transfection of siRNA 1 and 3 showed marked suppression of EGFR expression in NCI H1975 and PC9-G, however, siRNA 2 failed to suppress. All siRNA don`t affect EGFR expression in A549, NCI H460 and PC-9. EGFR T790M siRNA 1 and 3 not 2, markedly suppressed the growth of NCI H1975 and PC9-G. This finding strongly supports that EGFR T790M is another oncogenic driver mutation. Cotreatment of EGFR siRNA 1 and 3 with gefitinib induced marked increase in sensitivity of NCI H1975 and PC-9 to gefitinib (synergistic interaction). Application of EGFR T790M specific siRNA can reverse the resistance of lung adenocarcinoma and shows its potential to be a breakthrough in EGFR TKI. Further study will focus on preclinical application with efficient delivery system.Mutations in EGFR tyrosine kinase domain become driver oncogenic mutation in lung adenicarcinoma. EGFR TKI induces dramatic response in lung adenocarcinoma with sensitive mutations. Unfortunately, this dramatic response can not last long and resistance to EGFR TKI emerges and induces treatment failure. More than 50% of resistant mutations are EGFR T790M mutation. In this study, we investigated the role of siRNA specific to EGFR T790M. We designed three sequences (siRNA1, 2, 3) specific to EGFR T790M. Lung cancer cells were used: A549, NCI H460 (EGFR; wild type), NCI H1975 (EGFR L858R+T790M), PC9 (EGFR small deletion in exon 19), PC9-G (EGFR small deletion in exon 19+T790M). We investigated the effect of three siRNAs on suppression of EGFR T790M and reversal of resistance to gefitinib. Transfection of siRNA 1 and 3 showed marked suppression of EGFR expression in NCI H1975 and PC9-G, however, siRNA 2 failed to suppress. All siRNA don`t affect EGFR expression in A549, NCI H460 and PC-9. EGFR T790M siRNA 1 and 3 not 2, markedly suppressed the growth of NCI H1975 and PC9-G. This finding strongly supports that EGFR T790M is another oncogenic driver mutation. Cotreatment of EGFR siRNA 1 and 3 with gefitinib induced marked increase in sensitivity of NCI H1975 and PC-9 to gefitinib (synergistic interaction). Application of EGFR T790M specific siRNA can reverse the resistance of lung adenocarcinoma and shows its potential to be a breakthrough in EGFR TKI. Further study will focus on preclinical application with efficient delivery system.

암 환자의 가족지지와 희망에 관한 연구

전진호,어은영,손병철 인제대학교 1999 仁濟醫學 Vol.20 No.1S

암 환자에 있어서 가족의 지지와 환자 자신이 느끼는 병의 경과에 대한 희망은 암에 대한 대처 능력 확보와 삶의 질 추구에 중요한 요소이다. 암으로 진단 받고 자신이 암이라는 사실을 알고 있는 환자 147명을 대상으로 가족 지지도 관련 10문항, 환자 희망도 관련 15문항과 일반적 특성, 암의 진단 및 경과와 관련되는 특성 등의 설문 조사를 시행하였다 연구 대상자는 위암(29.9%), 간담도암(14.3%), 유방암(12.9%), 직장암(11.6%), 폐암(10.9%) 환자로 성별ㆍ연령별 분포의 차이는 없었으며 진단 경과 기간은 1∼3개월이 약 절반(42.9%)에 해당하였다. 치료 방법으로는 약물 요법(54.4%)이 가장 많았으며 주 간호 제공자는 배우자(55.8%)였다. 5점 척도로 환산한 가족 지지도와 환자 희망도의 전체 평균치는 가족 지지도 3.93±0.82. 환자 희망도 3.48±0.62였으며 가족 지지도와 환자 희망도 간에는 유의한 상관관계를 나타내었다(r=0.6338, p<0.01). 가족 지지도는 연령, 암의 종류, 치료 방법에 따라, 환자의 희망도는 통증의 유무, 연령, 진단 경과 기간에 따라 각각 유의한 차이를 보였다(p<0.05). 가족지지도는 50∼59세의 연령층이 다른 연령에 비하여 (p<0.05), 간담도암, 췌장암, 직장암 등이 다른 암종에 비하여(p<0.05), 약물ㆍ방사선 병행요법이 약물 또는 방사선의 단독요법에 비하여(p<0.05) 낮았다. 환자 희망도는 통증이 있는 경우가 없는 경우에 비하여(p<0.01), 50-59세의 연령층이 다른 연령에 비하여(p<0.05), 진단 경과 기간이 7∼12개월인 경우가 6개월 이하 또는 1년이상에 비하여(p<0.05) 낮았다. 암의 종류에 따른 가족 지지도와 환자 희망도는 대장암, 위암 등이 가장 높게 나타났다. 암 환자의 가족 지지도와 환자 희망도는 상호간에 밀접한 관련을 지니며, 연령, 암의 종류 통증의 유무, 진단 경과 기간 등에 따라 다를 수 있으므로 이러한 특성을 잘 이해하여 가족의 지지를 고취시키는 방향으로 암 환자 관리를 시도한다면 환자의 안녕과 대처 능력 증진에 도움을 줄 수 있을 것으로 여겨진다. For cancer patients, family support and perceived hope by himself is an important factor to fight against cancer and to enhance his own quality of life. This study was conducted to propose the basic data for nursing care that support well beings and quality of life for cancer patients through evaluating family support, perceived hope and their relationship. Questionnaire survey was executed on 147 cancer patients who recognized about his own diagnosis from one university hospital at Pusan during December, 1996. The data was analyzed by PCSAS(ver 6.11) with level of significance α=0.05. The subjects had no difference in gender and age distribution, and whose diagnosis was cancer of stomach(29.9%), hepatobiliary(14.3%), breast(12.9%), rectum(11.6%), lung(10.9%), etc. Around half of them(42.9%) was diagnosed within three months. and 54.4% took drug therapy, nursing care was mainly provided by spouses(55.8%). The mean score of the family support and perceived hope by five points scale was 3.93±0.82 and 3.48±0.62, respectively, and there was positive correlation between the two scores(r = 0.6338, p<0.01). For the family support, these was significant difference by age, type of cancer and type of therapy, and for the perceived hope, there was significant difference by age, presence of pain, interval after diagnosis (p<0.05). The mean score of family support was lower in the age group of 50-59, in the case of hepatobiliary, pancreas and rectum, and in the case of drug and radiology combined therapy than the other classification. The moan score of perceived hope was lower in painful case, in the age group of 50-59. and in seven to twelve months interval after diagnosis than the other classifications(p<0.05). And, the mean score of family support and perceived hope was higher in cancer of colon and stomach than the ethers by the type of cancer. In contrast that, there was no difference of family support and perceived hope by gender, marital status, religion, education level, occupation, income, number of family, main financial provider, main nursing provider, number of hospitalization, severity of pain, etc. In conclusion, there was positive correlation between family support and perceived hope, and it may be different according to age, type of cancer, presence of pain, interval after diagnosis, therefore, for the supportive and efficient care to cancer patients, it might be helpful to set up the care that can encourage family support.