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발전소용 스팀제어밸브와 유압서보액추에이터의 고장원인분석 및 개선에 관한 연구
이용범(Y. B. Lee),이기천(G. C. Lee),박종원(J. W. Park),김태석(T. S. KIM),이종직(J. J. LEE),양종대(J. D. Yang) 유공압건설기계학회 2019 유공압건설기계학회 학술대회논문집 Vol.2019 No.6
In nuclear and thermal power plants, turbine output control, a special steam valve that is opened with a hydraulic servo actuator and closed with a large steel spring, is used in high pressure and low pressure steam turbines. This steam control valve should supply an adequate amount of steam during normal power generation operation, but should protect the turbine rotating at high speed (Nuclear power plant 1800 rpm, thermal power plant 3600 rpm) by cutting off the steam supply quickly when an error occurs in the power generation system. This study analyzes the failure of the steam valve in the turbine output control system and the failure of the hydraulic servo actuator that operates the steam valve, and summarizes the results of the improvement.
Encephalomyocarditis Virus 표면항원의 단일항체 생산세포주의 크론과 이의 면역학 및 생화학적 연구(Ⅱ)
양종대,박종수,이영탁,김화영,김영래,이인수,조영준,박재윤,차종희,윤지원,고광삼 朝鮮大學校 附設 醫學硏究所 1987 The Medical Journal of Chosun University Vol.12 No.1
To see whether there is any differencies in RNA dependent DNA polymerase activities between monoclonal antibody-producing hydridoma cells and non-producing hybridoma cells, Balb/c female mice were immunized with the purified viral surface protein of D-variant of encephalomyocarditis virus and then fused with myeloma cells (NR-1). After cloring, monoclonal antibody-producing hybridoma cell lines were separated from non-producing hydridoma cell lines. RNA-dependent DNA polymerase activities were measured in the supernatant of monoclonal antibody-producing hybridoma clones and non-producing hybr idoma clones, and myeloma cells as control, Monoclonal antibody-producing hybridoma cells showed statistically significant higher activity as compar compare to that of nonproducing hybridoma cells. To find whether RNA-dependent DNA polymerase releasing cells aware secreting or budding C-type virus particles, those cells were examined with electron microscope. The hybridoma cell which secrete large amount of RNA-dependent DNA polymerase shows significant number of extracellular C-type virus particles. In constrast, non-producing hydridoma cells contains a lot of intracellular C-type virus particles. It is concluded that monoclonal antibody-producing hydridoma cells released particles. It is concluded that monoclonal antibody-producing hydridoma cells released significant amount of RNA-dependent DNA polymerase land extracellular C-type virus particles, while non-producing hydridoma cells showed less release of RNA-dependent DNA polymerase and contains intracellular C-type virus particles.