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The Proteomic Analysis of Human Placenta with Pre-eclampsia and Normal Pregnancy
양정인,공태욱,김행수,김호연 대한의학회 2015 Journal of Korean medical science Vol.30 No.6
Preeclampsia is one of the most important and complexed disorders for women’s health. Searching for novel proteins as biomarkers to reveal pathogenesis, proteomic approaches using 2DE has become a valuable tool to understanding of preeclampsia. To analyze the proteomic profiling of preclamptic placenta compared to that of normal pregnancy for better understanding of pathogenesis in preeclampsia, placentas from each group were handled by use of proteomics approach using 2DE combined with MALDI-TOF-MS. The 20 spots of showing differences were analysed and identified. Among differentially expressed protein spots Hsp 27 and Hsp 70 were selected for validation using Western blot analysis. In preeclamptic placenta 9 differentially expressed proteins were down-regulated with Hsp 70, serum albumin crystal structure chain A, lamin B2, cytokeratin 18, actin cytoplasmic, alpha fibrinogen precursor, septin 2, dihydrolipoamide branched chain transacylase E2 and firbrinogen beta chain. The 11 up-regulated proteins were fibrinogen gamma, cardiac muscle alpha actin proprotein, cytokeratin 8, calumenin, fibrinogen fragment D, F-actin capping protein alpha-1 subunit, Hsp 27, Hsp 40, annexin A4, enoyl-CoA delta isomerase and programmed cell death protein 6. The western blot analysis for validation also showed significant up-regulation of Hsp 27 and down-regulation of Hsp 70 in the placental tissues with preeclmaptic pregnancies. This proteomic profiling of placenta using 2DE in preeclampsia successfully identifies various proteins involved in apoptosis, mitochondrial dysfunction, as well as three Hsps with altered expression, which might play a important role for the understanding of pathogenesis in preeclampsia.
지방자치단체의 전자적 업무처리에서 저해요인과 활성화요인의 영향정도에 관한 연구
양정은(梁禎恩),정충식(丁忠植) 한국지방자치학회 2001 韓國地方自治學會報 Vol.13 No.2
The purpose of this article is to investigate on the significant factors of electronic management process focused on electronic approval and electronic document exchange in especially Local Government. The major factors facing when local government officials handled document electronically can be divided into four perspectives as follows; legal-constitutional, technological, cultural and individual perspective. The results showed that technological perspectives such as security, infrastructure and usage factor are the most barriers to the electronic management process. In the enabler factors, individual perspectives such as official ranking, human relation and computer literacy are the most important factors. Therefore, the following policy suggestions are recommended. First, security concept should be implemented by the local government. Second, the legal guidelines should be redefined. Third, our local government badly needs to improve its EDMS performance. Therefore, local government have adopted business process reengineering, Fourth, it is requested that information literacy training courses should be prepared in the local government.