랫드에서 Pueraria mirifica 추출물의 주산기 및 수유기시험

양세란(Se-Ran Yang),조성대(Sung-Dae Cho),조종호(Jong-Ho Cho),김경배(Kyung-Bae Kim),이지해(Ji-Hae Lee),안남식(Nam-Shik Ahn),정지원(Ji-Won Jung),박준석(Joon-Suk Park),이영순(Yang-Soon Lee),강경선(Kyung-Sun Kang) 한국환경성돌연변이발암원학회 2002 한국환경성돌연변이·발암원학회지 Vol.22 No.2

To evaluate the modifying effect of Kwao Kreu, Pueraria mirifica (PM) well-known as a rejuvenating folk medicine from Thailand, peri- and post-natal studies were carried out in rats. PM extract was administered to pregnant Sprague Dawley (SD) rats by oral gavage from gestation 6 (GD 6) to postnatal day 21 (PND 21). The amount of administered in this study was 0.042, 0.42 and 4.2 mg/kg/day, respectively. There were no treatment related changes of dams in deaths, clinical signs, and parturition. Treatment related changes in body weight, food consumption and lactation of dams were not observed. F1 fetuses in external abnormality, physical development, reflex/sensory functions and behavioral development were not found. No adults and F1 fetuses in organ weight was found with the exception of vagina and uterus of F1 fetuses. The results showed that PM extract, up to 4.2 mg, had no adverse effects on the peri- and post-natal development of rats. Therefore, PM extract has no adverse effects on peri- and post-natal development of rats.

전국의 공중화분 및 공중진균 포자 분포에 관한 연구

편복양(Bok Yang Pyun),김성원(Sung Won Kim),오재원(Jae Won O),이혜란(Hae Ran Lee),김정수(Jung Soo Kim),이경일(Kyung Il Lee),강임주(Yim Joo Kang),국명희(Myung Hee Kook),강혜영(Hae Young Kang),김진석(Jin Suk Kim),이명현(Myung Hyun Lee) 대한소아알레르기호흡기학회(구 대한소아알레르기 및 호흡기학회) 2000 소아알레르기 및 호흡기학회지 Vol.10 No.1

목 적 : 공중화분과 공중진균에 대한 분포 조사는 전세계적으로 이미 많이 연구되었고, 알레르기 질환과의 연관성에 대해서도 구미에서는 많은 연구가 이루어져 있다. 이는 알레르기 질환에 대한 기본 자료로서 절대적으로 필요하며 이를 기초로 역학적 조사가 이루어져야 함에도 불구하고 국내에서는 아직 전국적인 공중화분과 공중진균에 대한 분포 조사가 아직 미흡한 실정이다. 1960년대부터 현재까지 공중화분의 분포에 대하여 산발적이고 지역적인 보고가 있었으나 전국적인 역학적 분포 조사는 보고되지 못하고 있다. 이에 본 저자들은 1997년 7월 1일부터 1999년 6월 30일까지 2년간 전국의 공중화분과 공중진균의 분포를 조사하였기에 이를 보고한다. 본 연구는 대한소아알레르기 및 호흡기학회의 주관으로 이루어진 한국 어린이, 청소년 알레르기 질환에 관한 전국적 역학조사의 일환으로 조사가 시행되었다. 방 법 : 1997년 7월 t일부터 1999년 6월 30일까지 전국 10개 지역에 공중화분 및 진균포자 채집소를 정하여 가능한 동일한 조건을 얻기 위하여 모든 채집기(rotorod sampler; Sampling Technologies, Inc. MN)는 바닥으로부터 150 ㎝ 높이에 장치하여 매일 오전 8시부터 익일 오전 8시까지 24시간 동안 공중화분 및 공중진균을 포집한 후 매 2주마다 집결소(한양대 구리병원)로 보내어져 그곳에서 2명의 연구원에 의해 Calberla`s fuchs운으로 염색하여 100배 배율 하에서 공중화분을 관찰하고 400배 배율 하에서 공중진균을 관찰 동정하고 ㎥당 grain과 spore 수를 동정, 측정하였으며, 각 지역의 기상 관측소와 연계하여 채집 기간의 날씨(풍향, 기온, 습도 등)를 매일 기록하여 연관성을 비교하였다. 결 과 : 전국적으로 가장 많이 화분이 비산되는 시기는 8월 12일부터 9월 21일까지이었다. 지역별로는 전주지역에서 가장 많은 화분이 채집되었으며, 그 다음은 부산지역 순이었으며 대전, 대구, 여수지역은 비교적 적은 양의 화분이 채집되었다. 공중화분은 12월 중순부터 1월 중순까지는 발견되지 않았으며, 봄철 화분분포는 3월 7일부터 3월 30일까지 절정을 이루고 있었다. 지역별로는 부산지역이 가장 먼저 화분이 발견되었으며. 가장 많이 채집되었고, 그 다음으로 전주지역, 여수지역 순이었다. 8월부터 9월까지 두드러기쑥(Ambrosia)와 쑥(Artemisia) 가장 많이 나타났는데, 전주, 부산, 서울지역에서 많은 분포를 보였다. 공중진균은 7월 10일경부터 10월 24일까지 절정기를 이루었으며, 강우량과 비래하였으며, 장마철과 같이 아주 많은 양의 강수량이 있는 시기는 급격히 감소하였다가 비가 개인 다음날부터 많은 양의 진균포자가 비산하였다. 1998년 7월에 서울, 대전, 여수, 부산지역에서는 게릴라성 폭우로 인하여 강수량은 급증하였으나 공중진균의 양은 감소되고 있음을 보여주고 있다. 공중진균 포자의 비산은 11월부터 서서히 감소되며, 12, 1월에는 현격히 감소되지만 전국적으로 연중 발견되었다. 공중진균은 전국적으로 유사한 채집량을 보이고 있으며, 종류별로는 Cladosporium, Alternaria, Leptospheria의 ascospore 순으로 많이 발견되었다. Purpose : As outdoor allergen, pollen and mold are increasely drawing attention all over the country in Korea. The peninsula is located in a temperate zone and has four distinct seasons. In this study, we described the seasonal variation of the pollen and mold distributions in Korea, which may be used to evaluate the pollenosis prevalence for the first time. Methods : Rotorod samplers were installed on the 10 stations at each state in Korea. Outdoor allergens were collected daily in each station from July 1, 1997 to June 30, 1999. After staining they were identified, counted and recorded with the weather in each state. Results : The pollens were found in the beginning of February through the middle of December. The earliest pollen was found from Pusan which is a south region, and followed by Chonju, Daegu which are midsouth. The peak season for pollen was from the end of April to the middle of May(major pollen: Tree, Grasses), the second peak was depicted from the end of August to the middle of September (major pollen : sagebrush, ragweed) the outdoor molds were largely collected from the July to the middle of October. Cladosporium and Alternaria were prevalent all year round and Ascospores of Leptospheria was peak in monsoon season. Conclusion : The pollens were found during the whole year except from middle of the December through the middle of the January. Molds were found all year round.

국내 중국 유학생의 문화적응 유형에 따른 극복력과 건강상태

장혜경(Chang Hae-Kyung),한수정(Han Su-Jeong),양남영(Yang Nam-Young),유명란(Yoo Myoung-Ran),고은자(Ko Eun-Ja),김희경(Kim Hee-Kyung),임경춘(Lim Kyung-Choon),이미라(Lee Mi-Ra),손연정(Son Youn-Jung) 한국성인간호학회 2010 성인간호학회지 Vol.22 No.6

자기공명영상으로 선택된 환자에서의 대퇴전자간 회전 내전 절골술을 이용한 대퇴골두 무혈성 괴사환자의 치료

양진원 ( Jin Won Yang ),구경회 ( Kyung Hoi Koo ),송해룡 ( Hae Ryong Song ),정순택 ( Soon Taek Jeong ),박형빈 ( Hyung Bin Park ),이승환 ( Seung Hwan Lee ),정운하 ( Oun Hwa Chung ),조세현 ( Se Hyun Cho ) 대한고관절학회 1998 Hip and Pelvis Vol.10 No.2

We used MR(magnetic resonance) image to formulate new selection criteria for the identification of suitable patients with femoral head osteonecrosis for transtrochanteric rotational varizational osteotomy. The selection criteria included: [1] an early post-collapse osteonecrotic hip(loss of congruity or crescent sign) without narrowing of joint space or acetabular involvement; [2] an age of less than 55 years and a painful hip; [3] an angle of more than 120 of intact portion of the femoral head on midsagittal MR image. Seventeen patients met predefined selection criteria and were treated with the osteotomy. The angle of rotation was determined preoperatively in midsagittal MR image. The osteotomy included 20 of intentional varization. Postoperative scintigraphy showed adequate perfusion of the femoral head. They were followed for a period of 36 to 67 months(mean, 50 months). Hip score by Merle dAubigne et al improved from a mean of 13.4 preoperatively to a mean of 17.6 at the latest follow- up. Collapse of the feporal head was arrested in all hips and no case required a total hip arthroplasty. Use of MR images(kstead of plain radiograph) in the patient selection and in the determination of rotational angle may improve the success rate of the transtrochanteric rotational varizational osteotomy.

복합운동프로그램이 비만중년여성들의 생활습관병 관련인자와 혈중활성산소에 미치는 영향

양승자(Seung-Ja Yang),서영환(Young-Hwan Seo),오은해(Eun-Hae Oh),문경래(Kyung-Rae Moon) 한국발육발달학회 2018 한국발육발달학회지 Vol.26 No.2

Recently, due to lack of physical activity, middle-aged women are exposed to Lifestyle-related diseases by obesity and health care is urgently needed. The purpose of this study was to investigate the effect of complex exercise program on Lifestyle-related factors and blood free oxygen radical of middle-aged obese women. The two-way repeated measure ANOVA was used to verify the difference between the time of measurement and the group. At this time, the paired sample t-test was performed for the variables with statistically significant differences in each variable. The significance level of all results was set to p<.05 and the following conclusions were obtained. In the changes of Lifestyle-related factors, the body fat percentage showed an interaction effect between group and period & group. The blood glucose showed only the interaction effect between period & group. Total cholesterol had an interaction effect between timing and period & group, In neutral fat, there was only interaction effect between timing & group. In blood pressure, there was no interaction effect in time, group and time & group. Also in all the factors of the exercise group, the difference between the two groups was statistically significant after the experiments than before. Changes in blood activated oxygen were found to have an interaction effect between the two groups. In the exercise group, statistically significant decrease was observed after the experiment. In conclusion, complex exercise has a positive effect on improving Lifestyle-related factors and blood stress. This indicates that this can be a recommended exercise method for middle-aged obese women. In future follow-up studies, if the exercise duration is prolonged and the intensity is gradually increased, it can be considered to be a program that can prevent various Lifestyle diseases by eliminating middle-aged obesity.

코볼화일과 데이타베이스 설계 방법의 비교

양미아(Mi-a Yang),경연정(Youn-Jeong Kyung),이혜경(Hae-Kyung Lee) 한국정보과학회 1990 한국정보과학회 학술발표논문집 Vol.17 No.2

정보화 사회라 일컬어지는 현대사회에서 실세계의 데이타화와 그 관리는 매우 중요한 문제로 부각되고 있다. 그러나 아직 주류를 이루는 화일중심의 데이타관리는 그 운용에 있어 많은 문제점들을 갖고있다. 그로인한 과정들 속에서 대두된 개념이 데이타베이스 관리시스템이다. 자료들에 대한 그 중요성이 더욱 확대되면서 이 DBMS에 대한 관심은 더욱 증가하고 있다. 이에 우리는 시스템에 대한 설계를 중심으로, 코볼(COBOL) 프로그램언어를 통한 화일처리와 데이타베이스 개념을 사용하여 시스템을 설계하고, 이들을 비교함으로써 데이타베이스 설계가 어느 정도 실효성이 있는지 알아보고자 한다.

조기대퇴골두무혈성괴사에서 괴사범위와 대퇴골두함몰과의 관계

구경회 ( Kyung Hoi Koo ),송해룡 ( Hae Ryong Song ),정순택 ( Soon Taek Jeong ),김지연 ( Ji Yeon Kim ),양진원 ( Jin Won Yang ),하용찬 ( Yong Chan ),조세현 ( Se Hyun Cho ) 대한고관절학회 1995 Hip and Pelvis Vol.7 No.2

In a randomized trial conducted to compare the core decompression to the conservative treatment, we tested the hypothesis that the extent of necrosis at the intial magnetic resonance(MR) study predicts the subsequent risk of collapse of the femoral head. After the initial clinical evaluation incluing plain roentgenography and MR imaging, thirty-seven hips of early-stage osteonecrosis(ON) in thirtythree patients were randomly assigned to core decompression group or conservative treatment group. All the patients were regularly followed by clinical evaluation, plain roentgenography and MR imag- ing at three-month intervals. The extent of ON was estimated on the basis of the percentage of abnormal signal intensity in the weight-bearing portion of the femoral head as determined on a combination in coronal and sagittal MR images. The angle of necrotic portion in mid-coronal image(A) and that in mid-sagittal image(B) were used to quantify the extent of necrotic portion by the formular;(A/180)x(B/180)x100%. A strong correlation was observed between the percentage of necrotic portion and the development of collapse. We concluded that the extent of necrotic portion is a major risk factor of the collapse and proposed a systematic method of classifying the percentage of necrotic portion which might be useful as a predictive index for that fate of early-stage ON.

대퇴골두 무혈성 괴사에서 핵시감압술의 효과(무작위 선정에 의한 임상 실험)

구경회 ( Kyung Hoi Koo ),조세현 ( Se Hyun Cho ),소해룡 ( Hae Ryong Song ),정순택 ( Soon Taek Jeong ),김재수 ( Jae Soo Kim ),양진원 ( Jin Won Yang ) 대한고관절학회 1995 Hip and Pelvis Vol.7 No.1

A randomized trial was conducted on 33 patients' 37 hips with early-stage osteonecrosis (ON). After the initial clinical evaluation including plain roentgenography aad magnetic resonance (MR) imaging, hips were randomly assigned to a core decompression group or conservative treatment group. All the patients were regularly followed by clinical evaluation, plain roentgenography and MR imaging at intervals of three months. Hip pain was relieved in nine out of ten initially symptomatic hips in the core decompression group, while pain persisted in three out of four initially painfol hips in the conservatively treated group on the second evaluation (p<0.05). At a minimum follow-up of 24 months, 14 of the 1S eore decompressed hips (78%) and 15 of the 19 nonoperated hips (79%) devel- oped collapse of the femoral head. By survival analysis, there was no significant difference in the time to collapse between the two groups (log rank test p=0.79). Core decompression may be effective in symptomatic relief, but it may not provide additional benefit in preserving femoral head as compared to the conservative management in early-stage ON of the femoral head.

H Menthol (Nicotine Free-Tobacco Free) Herbal Cigarette의 흡입독성시험

강경선(Kyung-Sun Kang),조성대(Sung-Dae Cho),조종호(Jong-Ho Cho),김경배(Kyung-Bae Kim),이지해(Ji-Hae Lee),안남식(Nam-Shik Ahn),정지원(Ji-Won Jung),양세란(Se-Ran Yang),박준석(Joon-Suk Park),이영순(Yong-Soon Lee) 한국환경성돌연변이발암원학회 2002 한국환경성돌연변이·발암원학회지 Vol.22 No.2

Nowadays a huge variety of products that aim to assist to quit smoking or reduce addictive symptoms are developed and manufactured with safety evaluation, but the safety of the most recent products of interest which do not contain tobacco and nicotine, and shape cigarettes is not evaluated and guaranteed relatively. This study was carried out to evaluate the single and repeated dose inhalation toxicity and genotoxicity of H menthol (Nicotine free-tobacco free) herbal cigarettes provided by Cigastop Ltd. in ICR mice. In this study, doses which we determined to expose to mice were 40 cigarettes for 6 hours a day to mice in single dose and 20 (high dose), 10 (middle dose) and 5 cigarettes (low dose) a day for 28 days in repeated dose inhalation toxicity, in vivo chromosome aberration test and micronucleus test. The particulate substances from H menthol herbal cigarettes also were gathered and used in the Salmonella typhimurium/microsome assay (Salmonella test; Ames<br/> test). We could find neither significant changes between control and treatment groups nor dose-response effects of test material at all except serum Ca level of female middle dose treatment group in repeated dose inhalation toxicity test. In conclusion, H menthol herbal cigarettes, when applied clinically intended dose we used, might not show any toxic and/or mutagenic effect.

마우스에서 항암제 유발 호중구 감소에 대한 HM 10411의 회복촉진효과

강경선(Kyung-Sun Kang),제정환(Jeong-Hwan Che),김경배(Kyung-Bae Kim),이지해(Ji-Hae Lee),조성대(Sung-Dae Cho),조종호(Jong-Ho Cho),박준석(Joon-Suk Park),안남식(Nam-Shik Ahn),양세란(Se-Ran Yang),정지원(Ji-Won Jung),이영순(Yong-Soon Lee) 한국독성학회 2001 Toxicological Research Vol.17 No.2

Neutropenia is a major dose-limiting side effect of cancer chemotherapy. The therapeutic effect of HM 10411 was examined on neutropenia caused by anticancer agents. Neutropenia in normal ICR mice was induced by a single combined intraperitoneal injection of 130 mg/kg of cyclophosphamide (CPA), 4.5 mg/kg of doxorubicin (DXR), and 1mg/kg of vincristine (VCR) on day 0. Neutropenia in tumor-bearing mice was made by a single intraperitoneal injection of 200 mg/kg oj cyclophosphamide (CPA) into BALB/c mice bearing Colon 26 adenocarcinoma at 7 day after tumor implantation. HM 10411 or filgrastim (100 ㎍/kg/day) was subcutaneously administered for 5 consecutive days starting 1 day after injection of anticancer agents in order to stimulate neutrophil production. Injection of HM 10411 accelerated the recovery from these anticancer drug-induced neutropenia. In normal and tumor-bearing mice, neutrophil production efficacy of HM 10411 was similar than that of filgrastim. These results suggest that HM 10411 could be useful in the clinical treatment for neutropenia induced by anticancer agents.