Superoxide Dismutase와 Ascorbate Peroxidase가 엽록체내로 동시에 과대발현된 형질전환 담배의 제초제들에 대한 항산화 반응

김진석,이병회,권석윤,김윤희,김소희,조광연,Kim Jin-Seog,Lee Byung-Hoi,Kwon Suk-Yoon,Kim Yun-Hee,Kim So-Hee,Cho Kwang-Yun 한국식물생명공학회 2005 식물생명공학회지 Vol.32 No.2

Superoxide dismutase와 ascorbate peroxidase가 동시에 과대발현된 담배(CA)를 가지고 여러 제초제들에 대한 항산화 반응을 조사하였다. 온실조건 실험에서, CA 담배는 PSI 저해제로 알려진 paraquat처리에 대해서 내성이 인정되었고 그 정도는 40% 내외였다. PS II 저해제 (bromoxynil, diuron, bromacil), 엽록소 생합성 저해제 (oxyfluorfen), 카로티노이드 생합성 저해제 (fluridone)와 EPSP synthase 저해제 (glyphosate) 처리에서는 CA와 wild type간의 반응차이가 관찰되지 않았다. Paraquat와 diuron을 이용한 약광 조건의 실험에서도 온실조건의 실험결과와 유사한 정도로 paraquat처리에 대해서만 내성을 나타내었다. 온실조건에서의 엽위별 반응의 경우, 6 - 9 엽기 식물체에 paraquat를 처리하였을 때, 약제처리 당시 위로부터 3 - 4번째 전개되고 있었던 잎이 상대적으로 내성 정도가 높게 나타났다. 한편 paraquat 처리 시에 여러 농도의 ascorbic acid를 혼합할 경우, CA와 wild type 모두에서 비슷한 정도로 paraquat 활성을 경감시켰다. 결론적으로, CuZnSOD/APX의 과대발현은 photosystem I 에서 발생되는 산화스트레스에 대해서만 주로 작용하며, 다른 제초제들에 의해 발생되는 산화적 스트레스에 대해서는 소거 능력이 부족한 것으로 판단되었다. Antioxidative responses of transgenic tobacco plants expressing both superoxide dismutase (SOD) and ascorbate peroxidase (APX) in chloroplasts was investigated with several herbicides. In greenhouse test, tolerance of SOD/APX-overexpressed tobacco (CA) to photosystem (PS) I inhibitor paraquat was increased by about 40%. However, any response differences between CA and wild type (WT) tobacco was not observed in a treatment with PS II inhibitors (bromoxynil, diuron and bromacil), chlorophyll biosynthesis inhibitor(oxyfluorfen), carotenoid biosynthesis inhibitor (fluridone) and 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase inhibitor (glyphosate). This tendency was also similar in the growth chamber test of low light intensity, using paraquat and diuron. That is, increased antioxidant activity of CA was shown only in paraquat treatment. When paraquat was foliar-treated to 6 to 9-leaf stage plant, the third to fourth placed leaf from shoot tip showed relatively higher antioxidant activity. Ascorbate supplemented to paraquat solution alleviated the phytotoxicity with a similar range in both CA and WT. In conclusion, CA specifically responded to oxidative stress induced by paraquat among tested herbicides in a whole plant assay.

유한 이층 영상평면 구조에 의한 EMI 차폐성능 분석

김진석,윤재훈,이애경,김남,Kim, Jin-Suk,Yun, Jae-Hoon,Lee, Ae-Kyung,Kim, Nam 한국통신학회 2000 韓國通信學會論文誌 Vol.25 No.5

영상평면을 사용하면 인쇄회로기판(PCB)의 전자파 복사를 효과적으로 감소시킬 수 있다. 이 논문은 이층 유한 영상평면이 PCB 로부터 방출하는 불요 전자파에 미치는 영향을 분석한 것이다. 유한 영상평면 구조의 해석을 삼각형 patch 모델을 모멘트 기법에 적용하여 이루어졌다. 기존에 보편적으로 사용하던 삼각형 분할방법을 개선하여 표면의 경계에 있는 삼각형에서 두 개의 기저함수를 삼각형 분할방법을 제안하고 그 결과가 수치해석의 정확도에 미치는 영향을 비교분석하였다. EMI problem is very important in a printed circuit board design. This paper deals with image plane as one of the most effective method to reduce EMI in PCB. The shielding performance of one and two layers of image planes of finite width and length is analyzed by the method of moments based on the triangular surface patch model. The current distributions were modeled as two simple line sources. It is demonstrated that proper triangulation should be made so as to take two basis functions in the boundary triangles of the surface.

The effects of selenium on fetal growth and development in CD-1 mice exposed with mercury for the gestation period

김진석,이상목,최석화,이원창,Kim, Jin-suk,Lee, Sang-mok,Choi, Seok-wha,Lee, Won-chang The Korean Society of Veterinary Science 1994 대한수의학회지 Vol.34 No.2

Teratogenic and embryotoxic effects of mercury have been reported, however, there is little information about possible antidotes against mercury exposure during gestation. In order to evaluate therapeutic effects of selenium as an antidote against mercury poisoning, pregnant CD-1 mice were exposed to methylmercury chloride(20ppm) through the drinking water with treatment of sodium selenite (1.0mg, 2.0mg or 3.0mg/kg b.w., subcutaneously) or BAL(5.0mg/kg b.w., subcutaneously) under the single or combination base as the therapeutic agents from day 6 to 15 of gestation. Fetal growth parameters such as body weight and crown-rump length in the mice exposed to mercury, were reduced as was placental weight compared to those in the control. Treatment of selenium(alone, combination with BAL) reduced the harmful effects induced by mercury on the fetal growth parameters even though no specific relationship between dose and therapeutic effect. The incidence of dead fetuses/resorptions and malformed fetuses(especially cleft palate) was also increased in the mercury only treated group. Selenium treatment demonostrated reduced the incidence of abnormal fetuses under the exposure of mercury. Relative maternal organ weights(liver, kidney, spleen) were increased significantly but relative brain weight was decreased as evidenced by decreased in the mercury treated mice compared to that in the control. A subtle indication of maternal mercury toxicity evidenced by changes of relative maternal organ weights, decreased water and feed consumption were also prevented efficiently by selenium treatment. The present study suggests that methylmercuric chloride is embrytoxic and teratogenic in CD-1 mice when exposured during organogenesis and that selenium administration may have therapeutic application for the treatment of mercury poisoning although more applicable study in human should be performed with caution in the future.

유한 도전성 영상 평면이 PCB 전자파 복사에 미치는 영향 분석

김진석,조광윤,김남,Kim, Jin-Suk,Cho, Kwang-Yun,Kim, Nam 한국통신학회 1998 한국통신학회논문지 Vol.23 No.4

In this ppaer, the confguration of multi-layers of the image planes surrounding the current trace of inite width has been considered, and the shielding performance of image planes is analyzed with the parameters of the width and conductivity of the planes, and the distance between the current trace and the planes. The induced current distributions on the image planes have bgeen calculated by solving a set of electric field integral equations by the method of moments. To cacluate the current distribution on the planes effectively, a new method for dividing integral intervals is proposed.

측두하악관절과 저작근의 통증이 긴장성 두통에 미치는 영향

김진석,어규식,이진용,홍정표,전양현,Kim, Jin-Suk,Auh, Q-Schick,Lee, Jin-Yong,Hong, Jung-Pyo,Chun, Yang-Hyun 대한안면통증구강내과학회 2006 Journal of Oral Medicine and Pain Vol.31 No.4

Generally, Tension-Type Headache(TTH) patients exhibit muscle pain, but can also have TMJ pain, which includes mouth opening limitation or joint sounds. The purpose of our study is to observe the clinical pain characteristics between TTH patients with muscle pain and TMJ pain. One hundred sixty-seven patients were diagnosed with TTH according to the questionnaires based on the International Headache Society's proposal on the diagnostic criteria of TTH. The patients were classified into three group; arthralgia group (18 patients), myalgia group (50 patients) and arthromyalgia group (99 patients). TTH patients with pericranial muscle pain were classified in the myalgia group. TTH patients with temporal region pain were classified in the arthralgia group. TTH patients with both types of pain were classified in the arthromyalgia group. The parameters in the diagnostic criteria such as quality, intensity, laterality of pain, and aggravation due to physical activities were compared among the three groups. 1. There were no significant differences in the quality of pain among the three groups. 2. There were no significant differences in the intensity of pain among the three groups. 3. There were no significant differences in the laterality of pain among the three groups. 4. A higher percentage of patients in the arthromyalgia group experienced headaches that were aggravated due to physical activity (p=0.03) compared to the other groups. The results of this study show that TTH patients with both arthralgia (TMJ pain) and myalgia (pericranial muscle pain) are more aggravated by physical activity than TTH patients with either one.

차량 궤적 추적을 위한 불확실성 처리기 구현

김진석,김동호,류근호,Kim, Jin-Suk,Kim, Dong-Ho,Ryu, Keun-Ho 한국정보처리학회 2004 정보처리학회논문지D Vol.11 No.5

인터넷 기술의 발달로 다양한 응용분야의 컴퓨팅 환경은 상당한 변화를 겪어 왔다. 특히 지난 3년여 동안 물류분야에서는 물류 회사간 인터넷 기반의 가상 비즈니스 활동 또는 서비스 아키텍처를 의미하는 e-로지스틱스라는 새로운 개념에 대한 활발한 연구가 진행되었다. 효과적인 e-로지스틱스 프레임워크 구축을 위해서 GIS, GPS 및 시공간 데이터베이스를 포함하는 이동체 기술 개발이 진행 중에 있다. 이동체 기술은 자동차, 비행기, 선박 등과 같이 시간에 따라 공간상의 위치를 변경하는 시공간 객체에 대한 효율적인 데이터 관리를 의미한다. 그러나 기존의 관련 시스템은 여러 가지 이유로 이동체에 대하여 오직 가장 최근에 검출된 위치정보만을 관리하기 때문에, 과거 및 미래의 불확실한 위치 추정방법에 대한 구체적인 제시는 미약한 상태에 있다. 따라서 이 논문에서는 이동체에 대한 이력정보 관리와 이를 이용한 과거의 위치 추정이 가능한 시스템을 제안한다. 이를 위해 물류 운송차량 위치 추적을 위한 모델링과 데이터베이스 및 시스템 구조를 제시한다. 아울러 제안 시스템을 e-로지스틱스 분야의 주요 응용인 택배 차량에 대한 위치정보 불확실성 처리 시나리오에 적용하는 예를 가지고 설명한다. Along the advent of Internet technology, the computing environment has been considerably changed in many application domains. Especially, a lot of researches for e-Logistics have been done for the last 3 years. The e-Logistics means the virtual business activity and service architecture among the logistics companies based on the Internet technology. To construct effectively the e-Logistics framework, researches on the development of the Moving Object Technology(MOT) including GPS and GIS with spatiotemporal databases technique so far has been done The Moving Object Technology stands for the efficient management for the spatiotemporal objects such as vehicles, airplanes, and vessels which change continuously their spatial location along with time flows. However, most systems manage just only the location information detected lately by many reasons so that the uncertainty processing for the past and future location of the moving objects is still very hard. In this paper, we propose the moving object uncertainty model and system design for e-Logistics applications. The MOMS architecture in e-Logistics is suggested and the detailed explain of sub-systems including the uncertainty processor of moving objects is described. We also explain the comprehensive examples of MOMS and uncertainty processing in Delivery Parcel Application that is one of major application of e-Logistics domain.

Glyphosate에 대한 옥수수 반응의 개선된 검정방법

김진석,이병회,김소희,민석기,최정섭,Kim, Jin-Seog,Lee, Byung-Hoi,Kim, So-Hee,Min, Suk-Ki,Choi, Jung-Sup 한국식물생명공학회 2006 식물생명공학회지 Vol.33 No.1

본 연구에서는 옥수수를 대상으로 glyphosate에 대한 여러 가지 생리적 반응을 검토한 후, glyphosate 저항성 평가에 활용될 수 있는 보다 개선된 방법 두 가지를 확립하였다. 한 가지 방법은 옥수수 제3엽 상단에 약제를 국소 처리한 다음, 처리 후 3일째에 약제처리 되지 않았던 제4엽의 신장 정도를 조사하는 것이다 (전식물체-엽생장 검정). 이 경우 glyphosate $50-1,600{\mu}g/mL$ 범위에서 농도가 증가됨에 따라 엽 생장이 억제되었으며, $1,600{\mu}g/mL$ 농도에서의 생장 억제율은 무처리 대비 55.5%였다. 다른 한 가지 방법은 옥수수 제3본엽의 엽절편 ($4{\times}4mm$) 4개씩을 $200{\mu}L$의 시험용액이 담긴 48 well plate에 치상한 후 $25^{\circ}C$ 연속 명조건에 24시간동안 배양하여 shikimate 축적량을 조사하는 것이다 (엽절편-shikimate 축적 검정). 이 경우 시험용액에 0.33% sucrose를 가하면 무첨가에 비해 약3-4배 정도의 shikimate 축적 증가가 관찰되었고 glyphosate $2-8{\mu}g/mL$ 농도범위에서 직선적 증가반응을 나타내어 기존방법 (Shaner et al. 2005)보다 개선된 특징을 보였다. 본 방법들은 glyphosate 저항성 옥수수를 창출할 때 또는 저항성 유전자의 타 식물로의 이동여부와 잡초화된 저항성 옥수수 존재여부를 감별하는데 활용될 수 있을 것이다. 이 때 glyphosate에 대한 저항성 원인이 작용점 EPSPS와 관련이 있는 경우에는 "엽절편-shikimate 축적 검정"이 가장 바람직하고, 저항성 원인이 체내이행 감소 때문일 경우에는 "전식물체-엽생장 검정" 수행이 필요하다. Several methods for determining the response of corn to glyphosate were investigated to provide a fast and reliable method for identifying glyphosate-resistant corn in vivo. Two bioassays were developed. One assay is named 'whole plant / leaf growth assay', in which the herbicide glyphosate is applied on the upper part of 3rd leaf and the growth of herbicide-untreated 4th leaf is measured at 3 day after treatment. in this assay, the leaf growth of conventional corn was inhibited in a dose dependent from 50 to $1600{\mu}g/mL$ of glyphosate and growth inhibition at $1600{\mu}g/mL$ was 55% of untreated control. The assay has the potential to be used especially in the case that the primary cause of glyphosate resistance is related with a reduction of the herbicide translocation. Another assay is named 'leaf segment / shikimate accumulation assay', in which the four excised leaf segments ($4{\times}4mm$) are placed in each well of a 48-well microtiter plate containing $200{\mu}L$ test solution and the amount of shikimate is determined after incubation for 24 h in continuous light at $25^{\circ}C$. In this assay, 0.33% sucrose added to basic test solution enhanced a shikimate accumulation by 3 to 4 times and the shikimate accumulation was linearly occurred from 2 to $8{\mu}g/mL$ of glyphosate, showing an improved response to the method described by Shaner et al. (2005). The leaf segment / shikimate accumulation assay is simple and robust and has the potential to be used as a high throughput assay in the case that the primary cause of glyphosate resistance is related with EPSPS, target site of the herbicide. Taken together, these two assays would be highly useful to initially select the lines obtained after transformation, to investigate the migration of glyphosate-resistant gene into other weeds and to detect a weedy glyphosate-resistant corn in field.

The effect of thiamin on fetal growth and development in CD-1 mice exposed with mercury for the gestation period

김진석,최석화,Kim, Jin-suk,Choi, Seok-wha The Korean Society of Veterinary Science 1994 대한수의학회지 Vol.34 No.1

Pregnant CD-1 mice were exposed to methylmercury in the drinking water at concentration of 20ppm with subcutaneous treatment of thiaminHCl(vitamin $B_1$) (100mg, 200mg or 300mg/ kg b.w.) or BAL(5.0 mg/kg b.w.) under the alone or combined base at the therapeutic agents from day 6 to 15 of gestation. Fetal growth parameters, including body weight and crown-rump length in the mice exposed to mercury, were reduced as placental weight compared to those in the control group(no treatment). The incidence of dead fetuses/resorption and malformed fetuses(especially cleft palate) was also increased even in the group treated with thrapeutic agents as well as in the mercury only treated group. However, all kinds of alteration indicated above, possibly induced by mercury, reduced/or decreased significantly compared to those of control. A subtle indication of maternal toxicity was noted in most experimental animals as evidenced by decreased water consumption and increased relative liver weight. The present study confirmed that methylmercuric chloride is embrytoxic and teratogenic in CD-1 mice when administered during organogenesis and that thiamin administration may have therapeutic application for the treatment or prevention against of deleterious effects induced by mercury during gestation period.

뇌조직 재응집배양을 이용한 알루미늄과 Superoxide Dismutase(SOD)의 약리독성학적 효능 평가

김진석(Jin Suk Kim),오동환(Dong Hwan Oh),조대현(Dae Hyun Cho) 한국예방수의학회 1995 예방수의학회지 Vol.19 No.1

환경물질 중에는 낮은 농도에서 장기간 노출될 경우 신경계(특히 중추신경계)에 심한 신경 독성요인이 되는 경우가 많으나, 기술적 및 경제적인 문제 때문에 정확한 신경독성학적인 평가에 많은 어려움이 있다. 이러한 이유 때문에 좀 더 간단하며 이용이 용이하고 정확한 실험실내의 신경독성물질 검색방법을 확립하기 위하여 이미 신경독소로서 그 성질이 확립되어 있는 알루미늄 (0.1%)과 이론적으로 알루미늄과 생화학적 상호작용이 예상되는 superoxide dismutase(SOD) (6.8, 13.6 I.U./kg)를 임신기간 동안 투여받은 Sprague-Dawley 랫트 어미로부터 제왕절개술로 태아를 얻어 그들의 신체조건의 측정과 형태학적인 관찰을 통하여 우선 알루미늄과 SOD의 기형 독성에서의 상호작용을 살펴본 후, 전뇌를 더 이상의 알루미늄과 SOD 투여없이 재웅집배양을 실시하고, 다른 한편으로는 임신기간동안에는 투여하지 않은 태아의 전뇌를 이용하여 배지에 알루미늄 (0.1, 0.25, 0.5, 0.75, 1.0mM)과 SOD(2.0, 4.0, 6.0, 8.0, 10.0mM)를 시험적 화학물질로 선택하여 재응집세포배양을 실시하여 그 결과를 평가하였다.

특별강연(特別講演) 및 학술연구(學術硏究) 발표요지(發表要旨) : 제2분야: 잡초 생리생태 ; 화본과 식물에 대한 glyphosate 저항성 검정법

김진석 ( Jin Seong Kim ),이병희 ( Byung Hoi Lee ),김소희 ( So Hee Kim ),민석기 ( Suk Ki Min ),최정섭 ( Jung Sup Choi ),황인택 ( In Taek Hwang ) 한국잡초학회 2006 한국잡초학회 별책(학술대회 초록집) Vol.26 No.1