잇솔질 교육방법이 구강위생관리능력에 미치는 영향

안현희 ( Hyun Hee An ),이명희 ( Myung Hee Lee ),서화정 ( Hwa Jeong Seo ) 한국보건정보통계학회 2012 보건정보통계학회지 Vol.37 No.1

Objectives: The purpose of this study is to find out the difference of oral hygiene performance ability according to toothbrushing instruction methods. Methods: We conducted survey and patient hygiene performance index teston 150 patients, who visited one of 3 dental clinics in the national capital region and did not have any oral disease. Results: This study conducted logistic regression analysis to find out major factors affecting oral hygiene performance ability. The subjects in B group, trained by direct instruction of toothbrushing practice, showed a statistically significant difference as they were 3.08 times more likely to have oral hygiene performance ability above the average, compared to A group, who were trained by dentiform model. Moreover, the subjects in C group, trained by toothbrushing instruction with disclosing solution, also showed statistically significant difference by being 4.13 times more likely to have oral hygiene performance ability above the average compared to A group. Conclusions: According to the aforementioned results, C group was enhanced the most, so it was proved that toothbrushing instruction with disclosing solution was the most effective method to improve oral hygiene performance ability, which guarantees voluntary management of one`s oral hygiene.

침지형 막분리 공정의 막오염 요인 연구 ( 1 )

신항식(Hang Sik Shin),안현희(Hyun Hee An),강석태(Seok Tae Kang) 한국물환경학회 1999 한국물환경학회지 Vol.15 No.3

Among several factors related to the fouling of the submerged membrane bioreactor (SMBR) such as suction pressure, backwash cycle, MLSS, and ECP (exocellular polymer). ECP which is main component of activated sludge flocs has been reported as a major factor on fouling because it increases of viscosity in activated sludge and accumulates on the surface of membrane. In this study, the cake and plugging resistances caused by MLSS and ECP were measured to identify the influence of ECP on fouling quantitatively. It was found that the cake resistance by ECP occupied over 90% of total resistance. The ratio of protein-like ECP (ECPp) to carbohydrate-like ECP (ECPc) was an important fator to increase the cake resistance. In the operation of SMBR, it was needed to monitor the concentration of protein-like ECP and total ECP.

Conditioned Media of RAW 264.7 Cells Stimulated with Phellinus linteus Extract Regulates the Epithelial-mesenchymal Transition in Prostate Cancer Cells

Taewoo Kang(강태우),Hyun-Hee An(안현희),Sul-Gi Park(박슬기),Sun-Nyoung Yu(유선녕),You-Lim Hwang(황유림),Ji-Won Kim(김지원),Soon-Cheol Ahn(안순철) 한국생명과학회 2019 생명과학회지 Vol.29 No.8

전립선암은 전이성 종양 중의 하나로 치료를 위해 호르몬 요법이나 외과 적 거세 방법이 주로 수행되지만 많은 부작용을 나타내었다. 최근 많은 연구자들이 이러한 상황을 해결하기 위해 종양 미세 환경을 연구하고 있으며 그 중 면역 세포, 특히 대식세포는 종양 미세 환경의 중요한 구성요소이다. 정상적인 조건에서 대식세포는 여러 암세포에 대해 약한 종양 살균 활성을 갖으나 interferon-γ 또는 lipopolysaccharide에 의해 활성화되면, 염증성 사이토카인 및 케모카인을 분비함으로써 암세포를 직접 또는 간접적으로 사멸 시키게 된다. 본 연구에서는, 마우스 대식세포인 RAW 264.7 세포에 Phellinus linteus 추출물을 처리하여 산화질소의 방출과 pro-inflammatory cytokine들을 real-time PCR과 ELISA 방법으로 분석하였다. RAW 264.7의 조정 배지는 48시간 동안 전립선 암세포처리하여 상피간엽세포전이 관련 유전자의 발현을 측정 하였다. 그 때에 mesenchymal 관련 유전자들인 N-cadherin, snail, twist, slug 및 cadherin 11이 감소했을 뿐만 아니라 epithelial 관련 유전자인 E-cadherin은 증가하였다. 또한 암 전이 및 신생 혈관 형성에 관여하는 vimentin, ccl2 및 vegfa가 감소되었는데, 이는 EMT가 암세포의 이동과 침범에 밀접한 관련이 있기 때문이다. 따라서 Phellinus linteu에 의해 자극된 RAW 264.7 세포의 조정 배지는 인간 전립선 암세포주인 PC-3 세포의 이동과 전이를 억제하고 EMT 경로를 조절한다는 것을 나타낸다. Prostate cancer (PCa) is one of the most metastatic tumor. Although hormone therapy or surgical castration is mostly conducted to treat PCa, it has a lot of side effects. Recently, many researchers have been exploring the tumor microenvironment to remedy these circumstances. Immune cells, especially macrophages, are an important composition of the tumor microenvironment. Under normal conditions, macrophages exhibit mild tumoricidal activity against tumors. However, once activated by interferon gamma or lipopolysaccharides, macrophages can kill cancer cells directly or indirectly by secreting cytokines and chemokines. In this study, murine macrophage RAW 264.7 cells were treated with Phellinus linteus extract. To analyze their pro-inflammatory phenotype, we were used several assays such as a real-time polymerase chain reaction, an enzyme-linked immunosorbent and nitric oxide assay. Prostate cancer cells were treated with the RAW 264.7-conditioned media, which was identified as a pro-inflammatory nature, for 48 h, and the expression of epithelial-mesenchymal transition (EMT)-related genes was determined. Not only N-cadherin, Snail, Twist, Slug, and Cadherin 11, which are mechenchymal-related proteins, were decrease, but epithelial marker of E-cadherin was increased. In addition, the mRNA level of vimentin, ccl2, and vegfa were decreased, as the EMT is closely related to the migration and invasion of cancer cells. In conclusion, the RAW 264.7-conditioned media stimulated with P. linteus extract inhibited migration and invasion and regulated the EMT pathway in human prostate cancer cells.

견우자의 생리활성 분석과 추출물로부터 항암 활성물질의 분리

최현덕(Hyeun Deok Choi),유선녕(Sun Nyoung Yu),박슬기(Sul-Gi Park),김영욱(Young Wook Kim),남효원(Hyo Won Nam),안현희(Hyun Hee An),김상헌(Sang Hun Kim),김광연(Kwang-Youn Kim),안순철(Soon Cheol Ahn) 한국생명과학회 2017 생명과학회지 Vol.27 No.2

견우자는 한의학에서 사하 작용 및 이뇨 작용 등의 치료 목적으로 사용되어 왔다. 본 연구에서는 견우자를 methanol로 추출하고(PNM), 이를 기본으로 ethyl acetate 분획물(PNE), butanol 분획물(PNB), water 분획물(PNW)로 나누었으며 각 분획물을 이용하여 mushroom tyrosinase 저해활성, pancreatic lipase 저해활성, DPPH free radical 소거활성 및 암세포주의 증식 저해활성 등을 조사하였다. 특히, 전립선암 세포주 PC-3 세포에서 강력한 세포 증식 저해활성을 보였으며, 용매 분획물 중, butanol 분획에서 가장 높은 암세포의 증식 저해활성을 보였다. 따라서 견우자의 methanol 추출물을 Diaion HP-20 column chromatography, reverse phase column chromatography, Sephadex LH-20 column chromatography를 순차적으로 실시하여 최종적으로 저해활성이 가장 높은 compound PN을 얻었으며 HPLC를 통해 그 순도를 확인하였다. Compound PN을 이용하여 cell cycle arrest과 annexin V/PI를 측정한 결과, 세포의 sub-G1기를 축적되면서 apoptosis가 유발되어 전립선암 세포주 PC-3 세포의 세포사멸이 유도되는 것을 확인하였다. 따라서 본 연구를 통하여 견우자에 대한 항산화 활성, 미백 활성 등의 효과 뿐 만 아니라 항암 활성에도 활용할 수 있을 것으로 사료된다. This study aimed to evaluate several biological activities of Pharbitis nil and to isolate an anticancer agent from its methanol extract. Pharbitis nil seeds were extracted with methanol (PNM). Then, PNM was fractionated into solvent layers such as ethyl acetate fraction (PNE), butanol fraction (PNB), and water fraction (PNW). The biological activities of the fractions were analyzed for tyrosinase inhibition, lipase inhibition, DPPH-free radical scavenging, and cell growth inhibition. PNM showed strong growth inhibition of prostate cancer PC-3 cells. PNM was subjected to Diaion HP-20 and eluted stepwise with 50%, 80%, and 100% methanol. Then, for activity-guided fraction, each fraction was analyzed for growth inhibition of prostate cancer PC-3 cells by using an MTT assay. Because the 100% fraction showed significantly strong inhibitory activity, the fraction was further separated in the reverse phase C18, which was eluted with 80% and 90% methanol. The 90% fraction was further subjected to Sephadex LH-20 using a mobile solvent of 100% methanol. Finally, the compound PN was partially purified for HPLC analysis. PN showed cell growth inhibitory activity and induced the apoptosis and cell cycle arrest of prostate cancer PC-3 cells, as measured by flow cytometry. The results together suggest that Pharbitis nil possesses various biological activities, especially the inhibitory activity for the proliferation of prostate cancer PC-3 cells, suggesting the possibility of its use as an anticancer agent.

흰쥐의 시상하부내에서 Vasopressin과 Oxytocin 분비세포의 미세구조에 대한 면역세포화학적 연구

정주현,장인엽,안현희 朝鮮大學校 附設 醫學硏究所 1993 The Medical Journal of Chosun University Vol.18 No.1

An immunocytochemical study was performed using the specific monoclonal antibodies against arginine vasopressin (VP) and oxytocin (OT) to demonstate vasopressinergic and oxytocinergic neurons in the hypothalamus of the rat. 1. The oxytocinergic and vasopressinergic neurons were observed in both supraoptic and paraventricular nuclei. 2. The shapes of OT and VP nuclei were round, ovoid and spindle-shaped and its diameters of every shapes were 15-20 um. 3. In the supraoptic nuclei, numbers of oxytocinergic nuclei were about 667 and numbers of vasopressinergic nuclei were abserved than oxytocinergic nuclei. 4. In the paraventricular, unmbers of oxytocinergic nuclei were about 68 and numbers of vasopressinergic nuclei were about 804 and so more vasopressinergic nuclei were observed than oxytocinergic nuclei. 5. The electron microscopic observations were rough endoplasmic reticulum mitochondria, Golgi apparatus and microtubules in the VP and OT nuclei.