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골수의 Polyol 및 Sugar Dehydrogenase에 관한 연구
안병문,이희성,이근배,Ahn, Byoung-Moon,Lee, Hi-Sung,Lee, Keun-Bai 생화학분자생물학회 1979 한국생화학회지 Vol.12 No.1
토끼 골수의 homogenate를 분별원심 분리하여 세포질, mitochondria 및 핵분획을 분리하여 sorbitol dehydrogenase, adonitol dehydrogenase 및 mannitol dehydrogenase의 분포를 관찰하였으며 aldose 인 여러 당류에 대한 sugar dehydrogenase의 존재유무를 관찰하여 다음과 같은 결과를 얻었다. 1. Sorbitol을 D-fructose로, D-fructose를 sorbitol로 전환시키는 sorbitol dehydrogenase는 골수에 있어서는 D-fructose를 sorbitol로 촉매하는 작용만을 하며 mitochondria에만 존재한다. 2. Adonitol(ribitol) dehydrogenase의 활성도는 조직 1g 당 약 196 unit로 sorbitol dehydrogenase 및 mannitol dehydrogenase 보다 2.6배 및 41배 높았으며 세포질, mitochondria 및 핵분획에 각각 분포되어 있다. 3. Mannitol dehydrogenase는 조직 1g 당 4.78 unit로 활성도가 낮으며, 이 중 약 88%인 4.2 units가 세포질에 함유되어 있다. 4. Sugar dehydrogenae는 기질에 따라 특이성을 나타냈다. 즉, 조직 1g 당 효소활성도는 rhamnose>xylose>lactose>glucose>mannose>galactose>maltose의 순이며 rhamnose는 활성도가 20.14 unit/g으로 가장 높았고 trehalose에 대한 활성도는 전혀 없었다. Polyol dehydrogenases, i.e., sorbitol dehydrogenase, adonitol dehydrogenase and mannitol dehydrogenase of rabbit bone marrow were studied. Bone marrow was homogenized and the distribution of the enzyme activity in cytosolic, mitochonrial and nuclear fractions was measured. Sugar dehydrogenase activity in the crude preparations was also observed. The results obtained were as follows: 1. Sorbitol dehydrogenase activity which reduces D-fructose in the presence of NADH to D-sorbitol was localized in the mitochondria) fraction. The reverse reaction was inactive in both cytosolic and mitochondria) fractions. 2. Adonitol (ribitol) dehydrogenase activity (196 units/g wet tissue) was found to be approximately 3 times higher than that of sorbitol dehydrogenase and 40 times higher than mannitol dehydrogenase. 3. Mannitol dehydrogenase activity was very low (4.2 units/g wet tissue) and 88% of total activity was found in cytosolic fraction. 4. Sugar dehydrogenase were also examined in the crude preparation of bone marrow. he enzyme was most active with rhamnose (20.14 units/g wet tissue), but a number of other sugars was also good substrate. Dehydrogenation was effective in the following order of activity: rhamnose>xylose>lactose>glucose>mannose>galactose>maltose Trehalose did not serve as substrate.
골수의 Polyol 및 Sugar Dehydrogenase 에 관한 연구
안병문,이희성,이근배 ( Byoung Moon Ahn,Hi Sung Lee,Keun Bai Lee ) 생화학분자생물학회 1979 BMB Reports Vol.12 No.1
Polyol dehydrogenases, i.e., sorbitol dehydrogenase, ado:nitol dehydrogenase and mannitol dehydrogenase of rabbit bone marrow were studied. Bone marrow was homogenized and the distribution of the enzyme activity in cytosolic, mitochonrial and nuclear fractions was measured. Sugar dehydrogenase activity in the crude preparations was also observed. The results obtained were as follows: 1. Sorbitol dehydrogenase activity which reduces D-fructose in the presence of NADH to D-sorbitol was localized in the mitochondria) fraction. The reverse reaction was inactive in both cytosolic and mitochondria) fractions. 2. Adonitol (ribitol) dehydrogenase activity (196 units/g wet tissue) was found to be approximately 3 times higher than that of sorbitol dehydrogenase and 40 times higher than mannitol dehydrogenase. 3. Mannitol dehydrogenase activity was very low (4.2 units/g wet tissue) and 88% of total activity was found in cytosolic fraction. 4. Sugar dehydrogenase were also examined in the crude preparation of bone marrow. The enzyme was most active with rhamnose (20.14 units/g wet tissue), but a number of other sugars was also good substrate. Dehydrogenation was effective in the following order of activity: rhamnose $gt; xylose $gt; lactose $gt; glucose $gt; mannose $gt; galactose $gt; maltose Trehalose did not serve as substrate.