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추출 시간이 계내금(Galli stomachichum corium)의 항산화 및 단백질 효소 활성에 미치는 영향
이수아,박주현,심우등,서승명,김미숙,김태언,김교남,황지환 동아시아식생활학회 2024 동아시아식생활학회지 Vol.34 No.1
. Kyenegum (Galli stomachichum corium), or dried gizzard, is rich in protein and has long been used as a digestive aid. While previous research has focused on the pharmacological properties of Kyenegum, studies on its natural antioxidant and food additive potential have been limited. Therefore, this study aimed to investigate the antioxidant activity, protease activity, and total protein content of Galli Stomachichum Corium water extracts (GW) prepared using distilled water as a solvent. Various assays, including the total phenolic assay, 2,2-diphenyl-1-picrylhydrazy (DPPH) radical scavenging activity, copper reduction assay, Folin-Ciocalteu assay, and the Bradford protein assay, were performed. Kyenegum was extracted over different time periods (0 hr, 6 hr, 12 hr, 18 hr, and 24 hr). As a positive control for antioxidant activities, vitamin C (ascorbic acid) was used. The total phenolic content of GW significantly increased from 0 hr to 24 hr (0.93, 5.70, 8.20, 10.01, and 11.35 μg GAE/mL, at the above-mentioned time periods, respectively). The DPPH radical scavenging activity of GW was highest at 6 hr (12.42%) compared to GW extracted at other times. The protease activity and total phenolic content showed a time-dependent increase. Overall, these findings suggest that Kyenegum could serve as a natural antioxidant and food additive ingredient.