한의학의 발전 방향 검토

신길조,Shin, Gil Cho 대한중풍순환신경학회 2017 대한중풍.순환신경학회지 Vol.18 No.1

It is difficult to combine oriental and western medicine. Each medicine has a different academic background, perspective on the world, and studying methodology. The claim that two paradigms can not be combined is not obtained from an actual verification, but this means it is so hard to combine two medicines having different backgrounds. The amalgamation of oriental and western medicine should be phased in on the basis of the continuous reciprocal understanding and commitment. First, the strength and weakness of each medicine over the treatment and research are required to be identified. Then, a few complementary areas can be chosen enabling a trial of fusion on a small scale. A cycle of problem solving and a new research can be set by analyzing research results obtained through the implementation over a period of time. In other words, the researchers of oriental and western medicine should repeat a continuous and gradual complementary research process by identifying issues to be improved and complemented through a consensus. Once the methodology obtained through the process of problem solving and proficient implementation is established in a stable condition, a method to widen the fusion area by expanding the operating area and implementation method can be chosen. However, the integral system of oriental medicine shouldn't be substituted by mechanical idea or reductionism. What should be done primarily for oriental medicine is to objectify things through quantification. In particular, the oriental treatment should accept the microscopic diagnosis to determine the structure and observe the biochemical change.

흰쥐의 중대뇌동맥 결찰로 유발된 뇌허혈에서 양격산화탕(凉膈散火湯)이 신경세포에 미치는 효과

신길조,오경환,정승현,이원철,Shin, Gil-cho,Oh, Kyung-hwan,Jeong, Sung-hyun,Lee, Won-chul 대한중풍순환신경학회 2006 대한중풍.순환신경학회지 Vol.7 No.1

Objectives : Yanggyuksanhwa-tang is a prescription used for cerebral infarction clinically. Methods : According to previous research data, the effect of Yanggyuksanhwa-tang on cerebral infarction, we induced cerebral infarction by middle cerebral artery occlusion(MCAO) in rats, and the rats were administered Yanggyuksanhwa-tang. Results: Infarct area, infarct volume were measured, and the level of elements such as c-Fos, Bax and caspase-3 in penumbra of infarct were expressed by immunohistochemical staining. Conclusion : Yanggyuksanhwa-tang showed neuroprotective effect through preventing neuronal cell apoptosis.

흰쥐 대뇌세포의 저산소증 모델에서 석창포(石菖蒲 Acori graminei rhizoma. AGR)에 의한 유전자 표현 변화의 microarray 분석

신길조,박동준,정승현,문일수,이원철 한국생명과학회 2007 생명과학회지 Vol.17 No.2

Acori graminei Rhizoma (AGR) is a perennial herb which has been used clinically as a traditional oriental medicine against stroke, Alzheimer’s disease, and vascular dementia. We investigated the effect of AGR on the modulation of gene expression profile in a hypoxic model of cultured rat cortical cells. Rat cerebrocortical cells were grown in Neurobasal medium. On DIV12, cells were treated with AGR (10 ug/㎖), given a hypoxic shock (2% O2, 3 hr) on DIV14, and total RNAs were prepared one day after shock. Microarray analyses indicated that the expression levels of most genes were altered within the global M values +0.5 and -0.5, i.e., 40% increase or decrease. There were 750 genes which were upregulated by < global M +0.2, while 700 genes were downregulated by > global M -0.2. The overall profile of gene expression suggests that AGR suppresses apoptosis (upregulation of anti-apopotic genes such as TEGT, TIEG, Dad, p53, and downregulation of pro-apopotic genes such as DAPK, caspase 2, pdcd8), ROS (upregulation of RARα, AhR), and that AGR has neurotrophic effects (upregulation of Akt1, Akt2). These results provide a platform for investigation of the molecular mechanism of the effect of AGR in neuroprotection.

中風患者의 左·右半身不遂에 관한 臨床的 觀察

辛吉祚(Shin Gil-Cho),曺基湖(Cho ki-ho),金永錫(Kim Young-Suk),裵亨燮(Bai Hyoung-Sup),李京燮(Lee Kyung-Sup) 대한한의학회 1991 대한한의학회지 Vol.12 No.1

운동실조와 현훈을 주소로 한 소뇌경색 환자의 척담탕 치험 1례

최인영,김미경,김보람,최동준,한창호,정승현,신길조,이원철,Choi, In-Young,Kim, Mi-Kyung,Kim, Bo-Ram,Choi, Dong-Jun,Han, Chang-Ho,Jung, Seung-Hyun,Shin, Gil-Jo,Lee, Won-Chul 대한중풍순환신경학회 2008 대한중풍.순환신경학회지 Vol.9 No.1

Ataxia and vertigo are frequent clinical findings in cerebellar infarction patients. In severe cases, they cannot achieve normal activity in daily life. A 65-year-old man was admitted with ataxia and vertigo diagnosed as acute cerebellar infarction. We prescribed for Chukdam-tang to treat the phlegm disease. After this treatment, ataxia and vertigo improved. We suggest Chukdam-tang is significantly effective on the treatment of cerebellar infarction.

加味四物湯 및 加味四君子湯이 高脂血症 및 高血壓에 미치는 影響

辛吉祚,曺基湖,金永錫,裵亨燮,李京燮 경희대학교 1993 慶熙醫學 Vol.9 No.3-4

固眞飮子가 galactosamine으로 유발한 흰쥐의 간중독에 미치는 영향

원철환,임성우,윤상협,이원철,정승현,신길조 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 1997 東西醫學硏究所 論文集 Vol.1997 No.-

최근 간질환에 대한 진단과 기술의 비약적인 발전에도 불구하고 치료방법론에서는 그 해결이 모호한 상태에 직면해 있다. 실험적으로 간중독을 유발시킨 동물에 약물을 투여하여 그 악화를 입증하려는 노력이 진행되고 있다. 이에 저자는 固眞飮子가 간독성의 완화효과에 미치는 영향을 관찰하기 위하여 固眞飮子추출물을 투여한 흰쥐에 galactosamine으로 간독성을 유발하고 간조직내 glutathione과 과산화지질의 함량, 혈청중의 GOT, GPT, γ-GPT, ALT, LDH의 효소활성측정 및 혈청중의 bile acid함량을 측정하여 다음과 같은 유의한 결과를 얻었다. glutathione의 함량은 固眞飮子를 전처치한 실험군에서 유의성 있게 증가하였고, 과산화지질, 혈청중 GOT, GPT, γ-GPT, ALT, LDH, bile acid는 고진음자를 전처치한 실험군에서 유의성있게 감소하였다. The purpose of this study is to observe the protective effect of Kojinyumja on serum reaction and hepatic tissue in galactosamine treated rats. In this study, the experimental rats divided four group(Normal group, Control group, Sample A group, and Sample B group) : Under the same condition, normal and control group were administered water, sample A, B group were administered Kojinyumja for 8days. And then, both control group and Sample B group were injected to abdomen with galactosamine for 1day. The rates of of glutathione, lipid peroxide, GOT, GPT, γ-GTP, ALP, LDH, and contents of bile acid level were measured. The results are as follows : The glutathione rate significantly increased in sample group, the others (lipid peroxide, GOT, GPT, γ-GTP, ALP, LDH, bile acid) significantly decreased in sample group.

목향(木香) 및 사향(麝香)이 저산소증 유발 배양 대뇌신경세포에 미치는 영향

정승현,신길조,이원철,문일수,류도균,Jeong Sung-Hyun,Shin Gil-Cho,Lee Won-Chu,Moon Il-Su,Ryu Do-Kyun 대한한방내과학회 2003 大韓韓方內科學會誌 Vol.24 No.2

Objectives : The purpose of this investigation is to evaluate the effects of Aucklandiae Radix Moschus(木香 麝香)and to study the mechanism for neuronal death protection in hypoxia with Embryonic day 20 (E20) cortical cells of a rat (Sprague Dawley). Methods : E20 cortical cells used in this investigation were dissociated in Neurobasal media and grown for 14 days in vitro (DIV). On 14 DIV, Aucklandiae Radix Moschus(木香 麝香) was added to the culture media for 72 hrs. On 17 DIV, cells were given a hypoxic shock and further incubated in normoxia for another three days. On 20 DIV, Moschus(麝香)'s effects for neuronal death protection were evaluated by LDH assay and the mechanisms were studied by Bcl-2, Bak, Bax, caspase family. Results : This study indicate that Aucklandiae Radix(木香)'s effects for neuronal death protection in normoxia and Scutellariae Radix(麝香)'s effects for neuronal death protection in hypoxia were confirmed by LDH assay in culture method of Embryonic day 20(E20) cortical neuroblast. Moschus(麝香)'s mechanism for neuronal death protection in hypoxia is to increase the anti-apoptosis protein Bcl-2. Conclusions : It may be reasonable to propose that Moschus(麝香) protects delayed neuronal death in hypoxia by increasing Bcl-2, thereby reducing mitochondrial permeability transition(PT) pores, the cytochrome c channels.

배양한 흰쥐 대뇌세포의 저산소증 모델에서 황금(黃芩)이 유전자 표현에 미치는 영향

정승현,신길조,이원철,김성배,Chung, Sung-Hyun,Shin, Gil-Cho,Lee, Won-Chul,Kim, Sung-Bae 대한한방내과학회 2004 大韓韓方內科學會誌 Vol.25 No.4

Objectives : The purpose of this investigation is to evaluate the effects of Scutellaria baicalensis GEORGI on alteration in gene expression in a hypoxia model using cultured rat cortical cells. Methods : E18 rat cortical cells were grown in a Neurobasal medium containing B27 supplement. On 12 DIV, Scutellaria baicalensis GEORGI(20 ug/ml) was added to the culture media and left for 24 hrs. On 11 DIV, cells were given a hypoxic insult $(2%\;O_2/5%\;CO_2,\;37^{\circ}C,\;3\;hrs)$, returned to normoxia and cultured for another 24 hrs. Total RNA was prepared from Scutellaria baicalensis GEORGI-untreated (control) and -treated cultures and alteration in gene expression was analysed by microarray using rat 5K-TwinChips. Results : For most of the genes altered in expression, the Global M values were between -0.5 to +0.5. Among these, 1143 genes increased in their expression by more than Global M +0.1, while 1161 genes decreased by more than Global M -0.1. Effects on some of the genes whose functions are implicated in neural viability are as follows: 1) The expression of apoptosis-related genes such as Bad (Global M = 0.39), programmed cell death-2(Pdcd2) (Global M = 0.20) increased, while Purinergic receptor P2X(P2rxl) Global M = -0.22), Bc12-like1(Bc1211)(Global M = -0.19) decreased. 2) The expression of 'response to stress-related genes such as antioxidation-related AMP-activated protein kinase subunit gamma 1 gene (Prkag1) (Global M = 0.14), catalase gene (Global M = 0.14) and Heme Oxygenase(Hmoxl) increased. 3) The expression of Fos like antigen 2 (Fos12) expressed in neurons that survive ischemic insult increased (Global M = 0.97). Conclusions : these data suggest that Scutellaria baicalensis GEORGI increases the expression of antiapoptosis- and antioxidation- related genes in a way that can not yet be explained.

우황청심원(牛黃淸心元)이 저산소증 유발 배양신경세포에 미치는 영향

조규선,신길조,문일수,이원철,정승현,Cho, Gyu-Seon,Shin, Gil-Cho,Moon, Il-Soo,Lee, Won-Chul,Chung, Sung-Hyun 대한한방내과학회 2001 大韓韓方內科學會誌 Vol.22 No.2

Objectives : The purpose of this investigation is to evaluate the effects of Woohwangcheongsim-won on neuronal death of hypoxic E18 cortical neuroblast. Methods : To evaluate the effect of Woohwangcheongsim-won on neuronal death caused by hypoxia, the survival rate of E18 cortical neuroblast was measured with MTT assay and the changes of several synaptic proteins and enzymes were investigated with the immunoblot assays. Results : The E18 cortical neuroblasts were added 50, 100, 500, 1,000, and $5,000{\mu}g/ml$ Woohwangcheongsim-won. They showed neurotoxicity, when the concentration of Woohwangcheongsim-won was above $1,000{\mu}g/ml$. The E18 cortical neuroblasts, which were added 50, 100, and $500{\mu}g/ml$ Woohwangcheongsim-won, were exposed 98% $N_2/5%\;CO_2$ for 3 hours to induce hypoxia, 3 days later, the survival rate of $50{\mu}g/ml$ Woohwangcheongsim-won was 141.5% when compared to the control group. On the immuneblot assays, the expressions of ${\alpha}$CaMKII, NR2A, NR28, PDE2, PSD-95, and eEF-$1{\alpha}$ were increased in normoxia, but those of NR2A, NR2B were decreased in hypoxia when compared to the control group. Conclusions : The data shows that the effects of Woohwangcheongsim-won on neuronal death of hypoxic E18 cortical neuroblast is a significant result.