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한국인 만성 B형 간염 바이러스 ( HBV ) 보유자에서 HBV 유전자형 - 일차 중합 효소 반응법에 의한 온전한 B 형 간염 바이러스 증폭 및 그의 염기 서열 -
김학철(Haak Cheoul Kim),서검석(Geom Suk Seo),김용성(Youg Sung Kim),송우건(Woo Gun Song),문형배(Hyung Bae Moon),조지현(Jie Heun Cho) 대한내과학회 2001 대한내과학회지 Vol.61 No.5
N/A Background : Hepatitis B virus (HBV) is major source of chronic liver disease in Korea. However this virus might have different nucleotide sequence according to races, different region, etc. Recently the novel method that allows sensitive amplification with dramatically decreased mis-incorporation has developed. We determined to get the major form of HBV nucleotide sequence from whole sequencing data of 26 Korean HBV carriers. Methods : HBV DNA were collected from 26 Korean chronic HBV carriers. We used the novel PCR with pfu for the amplification of HBV DNA, and specific primers were made with combination sequence bases of non-HBV part and HBV parts which were located head and tail in the virion. Then whole length of HBV were directly sequenced and analysed. Result : HBV DNA was consisted of 3215 bases in 20 cases of 26 Korean chronic HBV carriers. And the remainder had smaller or larger number due to deletion, insertion or both in pre-S2 and S gene. They were 99.03% homology of their nucleotide sequence and belong to genotype C. The variability of nucleotide sequence was significantly higher in the singly coding region (SCR) than doubly coding region (DCR), and also high in pre-S1 and pre-S2 gene among the DCR. Hot-spots were more frequently found in the SCR, pre-S1 and pre-S2 gene. Conclusion : In Korean chronic HBV carriers, HBV is consisted of 3215 nucleotides, and belongs to genotype C. And it might exist one genotype with the variability in Korea.(Korean J Med 61:479-488, 2001)
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만성 B형 간염 바이러스 보유자에서 Core Promoter의 다양성
김학철,김용성,서검석,송우건 대한소화기학회 1999 대한소화기학회지 Vol.34 No.5
Background/Aims : The core promoter (CP) is included in X-gene of hepatitis B virus (HBV), and is composed of a upstream regulatory sequence (URS) and a basic core promotor (BCP) which is regulated by URS. Nucleotide (nt) mutation of CP might influence the expression of precore mRNA and pregenomic mRNA and the activity of liver disease. Thus, we investigated the relation between the nucleotide mutations in CP and their effects on HBeAg status, and severity of disease in chronic B viral liver disease. Methods : Samples were obtained from 7 chronic asymptomatic carriers, 9 patients with chronic hepatitis and 20 cirrhotic patients with HBV. Polymerase chain reaction and DNA analysis for CP were performed. Results : Cirrhotic group was older than asymptomatic carrier group and chronic hepatitis group. In BCP, point mutations were observed in 10 positions. Among them, nts 1753, 1762 and 1764 were frequently mutated, and the mutations at the nts 1762 and 1764 were accompanied (double mutation). In URS, point mutations were observed in 19 positions. Among these, nt 1653 (α-box) had significantly high mutation rate and the mutation at nt 1653 was closely associated with mutations at nts 1762/1764 in chronic liver disease and HBeAg (-) groups. Conclusions : The α-box of URS is closely related with double-mutation in BCP and HBeAg status in chronic B viral liver disease.
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