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담수 어류 혈청의 폴리펩타이드형 및 젖산 탈수소 효소 아이소 자임형에 관한 연구
宋容圭,李錦洙 충남대학교 자연과학연구소 1979 學術硏究誌 Vol.6 No.2
The serum proteins of sixteen different freshwater fish species which belong to seven different families were analyzed by polyaerylamide gel discontinuous electrophoresis. The polypeptide and LDH isozyme patterns were then examined on the basis of the phylogenetic relationship among species and the similarity of their life forms. 1. The polypeptide pattern of M,goreensisin Gobiobotiinae was similar to those of H.labeo, S.curriculus, and H.longirostris in Cyprininae which is belong to the same family, Cyprinidae. It suggests that the similarity of polypeptide pattern accords to the taxonomic classification. 2. The polypeptide pattern of P. asotus in Siluridae was similar to those of P,emarginatus and C.brevicorpus in Bagridae which have similar life forms. 3. The polypeptide pattern of A.iaponica in Anguillidae was similar to that of F.alba in Flutidae and that of P.emarginatus in Bagridae, They have similarity in their life forms. 4. The number of bands in the LDH isozyme pattern was always shown as 1, 2, 3, or 5, Since five bands are presented on the LDH isozyme patterns of P.herzi, and C.brevicorpus, the possible binomial distribution of two different polypeptide subunit in forming the LDH isozyme pattern still remains in the fish.
보리새우, Penaeus Japonicus 의 중장에서 일어나는 Glycine의 흡수과정에 관한 연구
宋容圭,李錦洙 충남대학교 자연과학연구소 1981 忠南科學硏究誌 Vol.8 No.1
Glycine transport mechanism through muscosal and serosal border of marine shrimp (P. Japonicus) midgut has been investigated. The transmural fluxes of glycine across the midgut wall were also examined. 1. Mucosal glycine influx process is composed of the active transport process with Na^+ requirement and the passive diffusion process. 2. Mucosal glycine uptake of midgut tissue in Na^+-free saline was significantly decreased as compared to that in normal saline. This result supports the idea of the co-transport mechanism of glycine with Na^+ on the mucosal border of the colummar epithelium. 3. L-alanine appeared to be a competitive inhibitor of mucosal glycine entry process. 4. Glycine accumulation process was significantly decreased by adding 1 mM IAA into the medium, suggesting that the most of glycine active uptake process was energy-dependent. 5. A tentative model of glycine transport in P.japonicus midgut is proposed.