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머루 줄기와 자소자로부터 분리한 Resveratrol 올리고머와 Flavonoid의 항균효과
손락호(Rak Ho Son),진휘승(Hwi Seung Chin),함아름(Ahrom Ham),마응천(Woongchon Mar),남궁우(Kung-Woo Nam) 대한약학회 2010 약학회지 Vol.54 No.1
We studied the antimicrobial activities of five compounds isolated from the stems of Vitis coignetiae Pulliat and the seeds of Perilla frutescens (L.) Britton. Based on spectroscopic evidence, compounds 1 to 5 were characterized as resveratrol, ε-viniferin, ampelopsin E, apigenin, and luteolin, respectively. The antimicrobial activities against Gram-positive (Staphylococcus aureus) and -negative (Pseudomonas aeruginosa) bacteria and a fungus (Candida albicans) were investigated using the disc diffusion and broth dilution methods. C. albicans was not inhibited by the five compounds. Compounds 2 and 5 had significant anti-microbial activity against S. aureus, and the 50% inhibitory concentration (IC50) of compound 2 against S. aureus was 7.2 μM. Compounds 4 and 5 significantly inhibited P. aeruginosa and the minimum inhibitory concentration (MIC) of compounds 2 and 5 was 0.07 and 2.0 μM, respectively. Compounds 2, 4, and 5 had strong anti-microbial activity against S. aureus and P. aeruginosa.
박송이,채규서,손락호,정지혜,임영례,권지웅 한국산업식품공학회 2012 산업 식품공학 Vol.16 No.4
본 연구에서는 2단계 발효를 통해 복분자 식초를 제조한 후 이에 대한 이화학적 성분 및 항산화 활성을 측정하였다. 복분자 식초의 알코올 발효 종료 후 알코올 함량은 11.78±0.03%이었고, 당도는 8.40±0.05 Brix이었으며, 초산발효 종료 후 알코올 함량은 0.16±0.03%이었고, 산도는 6.07±0.11%이었다. 복분자 식초의 주요 유기산은 acetic acid(4,641.59 mg%)와 citric acid(1,241.05 mg%)이었고, 복분자 식초의 총 폴리페놀, 총 플라보노이드 함량은 각각 38.01 mg/g, 17.91 mg/g이었다. 페놀화합물 중 ellagic acid 함량이 1,127.43 μg/g으로 가장 많았으며, gallic acid의 함량은 962.44 μg/g을 보였다. 복분자 식초의 DPPH 라디칼 소거 활성과 환원력은 500 μg/mL 농도에서 각각 60.3%, 0.50이었다. This study was performed to investigate the physicochemical properties and antioxidant activity of bokbunja (Black raspberry) vinegar by two step fermentation. The bokbunja vinegar was contained the highest amount of acetic acid (4,641.59 mg%) followed by citric acid (1,241.05 mg%) among the major organic acids in the vinegar. The total polyphenol and flavonoid contents in bokbunja vinegar were 38.0 mg/g and 17.8 mg/g, respectively. The ellagic acid and gallic acid contents in bokbunja vinegar were 1,127.43 μg/g and 962.44 μg/g, respectively. At concentration of 500 μg/mL bokbunja vinegar, the DPPH radical scavenging activity and reducing power were 60.3% and 0.50, respectively.
토종효모를 이용한 복분자 발효주의 바이오제닉아민 함량 및 향기성분 분석
윤해훈,손락호,류은혜,정지혜,Yoon, Hae-Hoon,Son, Rak-Ho,Ryu, Eun-Hye,Jung, Ji-Hye 한국식품조리과학회 2015 한국식품조리과학회지 Vol.31 No.3
We investigate black raspberry (Rubus occidentalis) wine made using traditional yeast (Saccharomyces cerevisiae A8, B6, GBY2, GBY3) and S. cerevisiae Fermivin (FM), which is widely used in wine manufacturing, and analyze the biogenic amine content and the volatile flavor compounds. Black raspberries were separately inoculated with yeast up to $1{\times}10^9CFU/kg$, followed by incubation at $25^{\circ}C$ for 7 days. FM produced the highest alcohol content, however the final fermentation characteristics of the wine made using four different yeasts were similar. S. cerevisiae A8 had a large biogenic amine (BA) content, specifically tryptamine, thus we excluded this yeast from fermentation. S. cerevisiae GBY3 was selected for black raspberry wine fermentation as a result of sensory evaluation. The volatile flavor compounds of two wines (S. cerevisiae GBY3 and FM) were analyzed by gas chromatography and mass spectrometry. 37 compounds in the samples were separated, and several ester compounds were identified in greater amounts in the wine made with S. cerevisiae GBY3 than in the wine made with FM. A greater amount of the major compound, ethyl benzoate, giving the sweet and fruity flavor, was identified in wine made with S. cerevisiae GBY3 than in the wine made with FM. In conclusion, S. cerevisiae GBY3 was confirmed to produce no major BAs and a better flavored wine. These results give new leads in the production of high quality wine.
진휘승(Hwi Seung Chin),손락호(Rak Ho Son),이용화(Yong-Hwa Lee),함아름(Ahrom Ham),마응천(Woongchon Mar),김원기(Won-Ki Kim),남궁우(Kung-Woo Nam) 대한약학회 2010 약학회지 Vol.54 No.2
The chemical structures of eight compounds purified from two plants (Polygala tenuifolia Willdenow and Dioscorea batatas Decene) were determined and their anti-microbial activity against three microbial strains (Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans) was tested. The three micro organisms were cultured in 96-well plates or Petri dishes without (control) or with the eight compounds added at concentrations of 100 to 0.01 μM (wt/vol). The growth of the microorganisms in the medium was examined after a 24-h incubation. The inhibitory effect of each compound on the growth of the microorganisms was calculated from the optical density measured at 595 nm, turbidity, and size of the inhibition zone around the treated paper disc. The minimum inhiitory concentration (MIC) of compounds 4 to 7 against S. aureus was 0.08, 0.05, 1.3 and 0.02 μM, respectively, and 0.09, 0.1, 0.2 and 100 μM against C. albicans. The IC50 (50% inhibition) values of compounds 5 and 6 were 3.1 and 6.4 μM against S. aureus, respectively, and 10 and 2.4 μM against C. albicans. Therefore, compounds 4 to 6 were the most potent anti-microbial agents among the eight compounds tested.
이응지 ( Eung-ji Lee ),배성윤 ( Seong Yun Bae ),손락호 ( Rak Ho Son ),이용화 ( Yong Hwa Lee ) 대한화장품학회 2009 대한화장품학회지 Vol.35 No.3
천연 미백 소재 개발을 위하여 복령피 추출물과 복령피 추출물에서 분리한 활성 물질의 멜라닌 생성에 연관된 생리 활성을 분석하였다. 복령피 추출물은 100 μg/mL 이하에서 세포 독성이 없는 것으로 확인 되었으며 free radical 소거능(DPPH)과 superoxide radicals 소거능 결과는 각각 IC<sub>50</sub> = 19.4 ± 2.21 μg/mL, IC<sub>50</sub> = 103 ± 3.33 μg/mL을 나타내었다. 50 μg/mL 농도에서 B16 melanoma 세포 내 tyrosinase의 활성을 34 % 저해 하였으며, 세포 내 멜라닌 생합성 저해 효과는 50 μg/mL 농도의 복령피 추출물을 72 h 동안 처리한 세포에서 51 % 억제율을 보이는 것으로 나타났다. 이러한 복령피 추출물의 활성 물질을 분리하여 1H-NMR, 13C-NMR, Mass analysis 등의 기기 분석을 실시한 결과 triterpene류의 3-β-hydroxylanosta-7,9(11),24-trien-4-oic acid로 동정되었고 100 μg/mL 이하에서 세포 독성이 없는 것으로 확인 되었다. 3-β-hydroxylanosta-7,9(11),24-trien-4-oic acid의 free radical 소거능과 superoxide radicals 소거능 결과는 각각 IC<sub>50</sub> = 4.3 ± 0.15 μg/mL, IC<sub>50</sub> = 54 ± 1.67 μg/mL을 나타내었다. 10 μg/mL 농도에서 세포내 tyrosinase의 활성을 43 % 저해 하였으며, 멜라닌 저해 효과를 확인한 결과 IC<sub>50</sub> = 3.6 μg/mL으로 나타났다. Western blot을 이용하여 tyrosinase, tyrosinase related protein-1 (TRP-1), TRP-2 단백질의 발현 감소를 확인하였다. 복령피 추출물과 3-β-hydroxylanosta-7,9(11),24-trien-4-oic acid는 우수한 미백 효능을 갖는 화장품 소재로의 개발 가능성이 클 것으로 기대된다. To develop a new natural whitening agent for cosmetics, we investigated the inhibitory effects of Poria cocos Bark extracts (PCBE) and its active compound on melanogenesis. PCBE showed ROS scavenging activities in 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and xanthine/xanthine oxidase system with the IC<sub>50</sub> values of 19.4 ± 2.21 μg/mL and IC<sub>50</sub> = 103 ± 3.33 μg/mL, respectively. PCBE reduced intracellular tyrosinase activity about 34 % at concentration of 50 μg/mL. And PCBE reduced melanin contents of B16 melanoma cells about 51 % at concentration of 50 μg/mL without cell cytotoxicity (below 100 μg/mL). We purified one active compound from PCBE and identified its structure. It was identified as 3-β-hydroxylanosta-7,9(11),24-trien-4-oic acid, triterpene family, by 1H-NMR, 13C-NMR and Mass analysis. 3-β-hydroxylanosta-7,9(11),24-trien-4-oic acid showed ROS scavenging activities in DPPH radical and xanthine/xanthine oxidase system with the IC<sub>50</sub> values of 4.3 ± 0.15 μg/mL and 54 ± 1.67 μg/mL, respectively. Also, it was shown that 3-β-hydroxylanosta-7,9(11),24-trien-4-oic acid reduced intracellular tyrosinase activity about 43 % at concentration of 10 μg/mL. And it inhibited melanin synthesis in a dose dependent manner (IC<sub>50</sub> = 3.6 μg/mL) without cell cytotoxicity (below 100 μg/mL). 3-β-hydroxylanosta- 7,9(11),24-trien-4-oic acid inhibited tyrosinase, TRP-1 and TRP-2 expression at protein level. These results suggest that PCBE and 3-β-hydroxylanosta-7,9(11),24-trien-4-oic acid reduced melanin formation by the inhibition of tyrosinase activity and expression in B16 melanoma cells. Therefore, we suggest that PCBE could be used as a useful whitening agent.