[indie. Special#3-독립영화인들의 반전을 위한 외침]폭력의 화염에 휩싸인 이라크, 그곳에 다녀와서

성혜란 한국독립영화협회 2003 독립영화 Vol.17 No.-

한국어의 모음배합의 유형 : 희곡작품을 중심으로

成惠蘭 이화여자대학교 1972 韓國語文學硏究 Vol.12 No.-

자동배양기를 이용한 미생물 검출

성혜란,김일회,김지연,이종길,정연복,한상배,송석길,Sung, Hye-Ran,Kim, Il-Hoi,Kim, Jee-Youn,Lee, Chong-Kil,Chung, Yeon-Bok,Han, Sang-Bae,Song, Suk-Gil 한국미생물학회 2008 미생물학회지 Vol.44 No.2

미생물 자동배양기를 이용한 감염성 물질의 검출은 시험적 오차의 경감은 물론 분리율 향상과 시간 단축을 가능하게 하였다. BacT/ALERT 3D 자동배양기는 미생물성장 시 발생되는 이산화탄소를 비색법으로 검출하는 장비로서 임상시료를 이용한 미생물 배양과 검출에 이용되어왔다. 자동배양기의 효율성을 검증하고 의약품의 무균 시험에 적용가능한지를 평가하기 위하여 6종의 세균을 이용하여 중식 및 검출특성을 분석하였다. 3종의 호기성세균 Pseudomonas aeruginosa, Micrococcus Iuteus, Bacillus subtilis와 Staphylococcus aureus는 1 CFU가 31.44 시간 내에 검출되었고, 혐기성균인 Clostridium sporogenes는 15.96시간에 균의 중식이 감지되었다. 저성장 혐기성세균인 Propionibacterium acnes는 $10^4$ CFU의 균수에서 검출에 129.36시간이 소요되었다. 이 같은 결과는전통적 직접 배양법보다 검출감도에 있어서 $10\sim10^5$배 높고 검출시간을 $2\sim10$10시간 단축한 것으로서 자동배양법이 미생물 증식과 검출에 효율적임을 말해준다. 따라서 본 시험에서 이용한 자동배양법은 임상시료에서의 감염원 진단뿐 아니라 생물의약품의 무균시험에 이용 가능하다고 판단된다. Modern automated culture systems have increased the isolation rate of microorganisms and shortened the time to detection, reducing experimental errors in diagnosis of infecting agents. BacT/ALERT 3D system is based on the colorimetric detection of $CO_2$ produced by the growing microorganisms. In order to evaluate the efficiency of the detection system, sterility test were performed using 6 bacteria. With standard aerobic and anaerobic bottles containing the liquid media, both three aerobic bacteria (P. aeruginosa, M. luteus, B. subtilis) and a facultative bacterium S. aureus were detected up to 1 CFU in 31.44 hr. In addition, growth of anaerobic C. sporogenes was recognized up to 1 CFU in 15.96 hr. The slowly growing bacteria P. acnes was detected up to 10,000 CFU in 129.36 hr. In comparison with conventional culture method, BacT/ALERT 3D automated culture system was more sensitive and saved detection time up to$2\sim10$ hr. Therefore, this automated culture system enables to efficiently detect bacteria in clinical samples and biological medicines.

다중 중합효소 연쇄반응을 이용한 DNA 바이러스의 동시검출

성혜란,주진영,이종길,정연복,송석길,Sung, Hye-Ran,Joo, Jin-Young,Lee, Chong-Kil,Chung, Yeon-Bok,Song, Suk-Gil 한국미생물학회 2007 미생물학회지 Vol.43 No.1

Epstein-Barr virus (EBV), cytomegalovirus (CMV), hepatitis B virus (HBV), parvovirus B19 (B19)등 4종의 바이러스는 인체에 감염을 일으키는 병원체로서 DNA를 유전물질로 함유한다. 각 바이러스 유전자의 염기서열을 분석하여 EBV CMV, HBV의 pol 유전자와 B19의 ns 유전자에 특이적으로 결합할 수 있는 primer를 설계 제작하고 단일 시험으로 4종의 바이러스를 동시에 검출할 수 있는 다중 중합효소 연쇄반응(Multiplex PCR)법을 확립하였다. Primer 염기서열, PCR 반응조성물의 농도, PCR 반응시간 및 온도조건을 최적화하여 민감도를 증대시킴으로써, 단일 시험으로 5-10 분자수의 유전물질까지 검출이 가능하였다. 또한 4종의 바이러스 사이에 교차반응이 일어나지 않았으며 생체시료를 이용한 시험에서도 특이성과 민감도가 유지됨을 확인하였다. 그러므로 본 연구에서 확립한 다중 중합효소 연쇄반응은 세포배양액 또는 생체 시료에 감염된 4종 DNA 바이러스진단에 효율적으로 이용할 수 있을 것이라고 판단된다. We describe a multiplex PCR method that can detect and differentiate simultaneously four different kinds of DNA viruses, Epstein-Barr virus (EBV), cytomegalovirus (CMV), hepatitis B virus (HBV) and parvovirus B19 (B19). Primers for the multiplex PCR reaction were designed to amplify specific regions of the EBV (pol), CMV (pol), HBV (pol) and B19 (ns) viral genomes and used to simultaneously detect individual viruses. In order to achieve optimal sensitivity and specificity for multiplex PCR, the thermo-cycling parameters, primer sequences, and concentration of each reaction components were optimized systematically. The sensitivity of the detection method ranged between 5 and 10 copies of viral genome with a mixture of multiple primer pairs. Furthermore, this highly sensitive test showed no cross-reactivity among the four viruses. Thus, the results obtained in this study provide evidence that the assay system is a good tool for supporting the diagnosis of viral infection and contamination.

PCR-Based Detection of Mycoplasma Species

성혜란,강승혜,배윤진,홍진태,정연복,이종길,송석길 한국미생물학회 2006 The journal of microbiology Vol.44 No.1

In this study, we describe our newly-developed sensitive two-stage PCR procedure for the detectionof 13 common mycoplasmal contaminants (M. arthritidis, M. bovis, M. fermentans, M. genitalium,M. hominis, M. hyorhinis, M. neurolyticum, M. orale, M. pirum, M. pneumoniae, M. pulmonis,M. salivarium, U. urealyticum). For primary amplification, the DNA regions encompassing the16S and 23S rRNA genes of 13 species were targeted using general mycoplasma primers. The primaryPCR products were then subjected to secondary nested PCR, using two different primerpair sets, designed via the multiple alignment of nucleotide sequences obtained from the 13 mycoplasmalspecies. The nested PCR, which generated DNA fragments of 165-353 bp, was found tobe able to detect 1-2 copies of the target DNA, and evidenced no cross-reactivity with the genomicDNA of related microorganisms or of human cell lines, thereby confirming the sensitivity and specificityof the primers used. The identification of contaminated species was achieved via the performanceof restriction fragment length polymorphism (RFLP) coupled with Sau3AI digestion. The results obtained in this study furnish evidence suggesting that the employed assay systemconstitutes an effective tool for the disagnosis of mycoplasmal contamination in cell culturesystems.

PCR-RFLP법을 이용한 마이코박테리아의 검출

성혜란, 김일회, 김지연, 이종길, 성석길 충북대학교 약품자원개발연구소 2008 약학논문집 Vol.23 No.-

본문참고

SSD1과 상호작용하는 유전자

성혜란, 배윤진, 송나리, 이종길, 송석길 충북대학교 약품자원개발연구소 2006 약학논문집 Vol.21 No.-

-본문참고-

결장암에 대한 활성 자연살해세포의 항암효능

성혜란(Hyeran Sung),김지연(Jee Youn Kim),박민경(Min Gyeong Park),김일회(Il-Hoi Kim),이동욱(Dong Wook Lee),한상배(Sang-Bae Han),이종길(Chong-Kil Lee),송석길(Sukgil Song) 대한약학회 2010 약학회지 Vol.54 No.3

Colorectal cancer is one of the most common alimentary malignancies. In this study, the antitumor activity of activated human natural killer (NK) cells against human colorectal cancer was evaluated in vivo. Human NK cells are the key contributors of innate immune response and the effective functions of these cells are enhanced by cytokines. Human peripheral blood mononuclear cells (PBMC) were cultured with interleukin-2 (IL-2)-containing medium for 14 days and resulted in enriched NK cell population. The resulting populations of the cells comprised 7% CD3+CD4+ cells, 25% CD3+CD8+ cells, 13% CD3-CD8+ cells, 4% CD3+CD16/CD56+ cells, 39% CD3+CD16/CD56- cells, and 52% CD3-CD16/CD56+ cells. Tumor necrosis factor alpha (TNF-α), interferon gamma (IFN-γ), IL-2, IL-4, and IL-5 transcripts of the activated NK cells were confirmed by RT-PCR. In addition, activated NK cells at doses of 2.5, 5 and 10 million cells per mouse inhibited 10%, 34% and 47% of SW620-induced tumor growth in nude mouse xenograft assays, respectively. This study suggests that NK cell-based immunotherapy may be used as an adoptive immunotherapy for colorectal cancer patients.

비소세포성폐암에 대한 자연살해세포의 항암효능

박민경(Min Gyeong Park),한상배(Sang-Bae Han),김지연(Jee Youn Kim),박지성(Ji-sung Park),성혜란(Hyeran Sung),윤병규(Byung Kui Yun),송석길(Sukgil Song),이종길(Chong-Kil Lee) 大韓藥學會 2011 약학회지 Vol.55 No.3

Human NK cells, identified 30 years ago based on their ability to spontaneously kill tumor cells, constitute a subset of lymphocytes, which play an important role in the first line of immune defense and the effective function of these cells are enhanced by cytokines. Lung carcinoma has been one of the most commonly diagonosed cancer as well as the leading cause of cancer death in male. Here we provide the evidence that human natural killer cells has inhibitory effects on tumor growth of human lung cancer cell NCI-H460 (non-small cell lung cancer). Enriched NK cell population was obtained by 2 weeks cultivation in interleukin-2(IL-2)-containing medium. The resulting population comprised 26% CD3+ cells, 9% CD3+CD4+ cells, 16% CD3+CD8+ cells, 76% CD56+ cells, 6% CD3+CD56+ cells and 70% CD3-CD56+ cells. Activated NK cells at doese of 2.5, 5, and 10 million cells per mouse inhibited 2%, 12% and 45% of NCI-H460-induced tumor growth in nude mouse xenograft assays, repectively. This result suggests that NK cell-based immunotherapy may be used as an adoptive immunotherapy for lung cancer patients.