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김현주 ( Hyun Joo Kim ),조은진 ( Eun Jun Cho ),이광호 ( Kwang Ho Lee ),김수배 ( Su Bae Kim ),배현종 ( Hyeun Jong Bae ) 한국목재공학회 2011 목재공학 Vol.39 No.1
본 연구는 새로운 전처리 방법인 팝핑법을 이용하여 폐목재에 처리하였고, 전처리 된 시료에 효소를 처리하여 당화 수율을 비교 분석하였다. 폐목재의 팝핑 전처리 전후의 화학적 조성 분석 결과 홀로셀룰로오스의 경우 각각 65.9%, 58.8%였으며, 리그닌과 알코올-벤젠을 이용한 유기용매 추출물, 회분의 함량은 전처리 전보다 각각 3.1%, 3.9%, 0.7% 정도 증가하였다. 그리고 폐목재의 팝핑 전처리 전과 후의 시료에 각각 효소 처리 후 환원당을 측정한 결과 팝핑 전처리를 한 경우 전처리 전보다 1.0~1.5mg/㎖ 증가하였다. 시료 50mg (1%, w/v)에 대한 효소 가수분해율은 셀룰레이즈와 자일란네이즈를 50U씩 l:l로 섞어 처리하였을 때 가장 효과적이었다. 또한 팝핑 된 시료에 효소를 처리하여 가수분해되어 나온 상등액을 HPLC 분석법을 이용하여 분석한 결과 팝핑 전처리 후 셀룰레이즈와 자일란네이즈를 처리하였을 때 피크가 증가하였으며, 주된 피크는 글루코스와 자일로스였다. 또한 GC 분석법을 이용하여 팝핑 후 효소가수분해 한 잔여물의 중성당 분석 결과 잔류 글루코스와 자일로스가 각각 팝핑 전보다 57.5%, 64.2% 감소하였다. 글루코스와 자일로스 당전환율은 각각 약 45.9%, 38.7% 였다. We have investigated pretreatment of waste wood using milling refinery combined with poping method, which can save energy for pretreatment and enzyme loading for enzymatic hydrolysis. The chemical analysis of holocellulose of non and popping treated waste wood showed 65.9% and 58.8%, and the lignin, organic extracts and ash were increased by 3%, 4% and 0.7% after pretreatment, respectively. The reducing sugar yields of pretreated waste wood were increased four times more than non-pretreated one and the synergistic effect of cellulase and xylanase were evaluated compare with individual enzyme treatment. Especially, enzyme cocktail (cellulase 50U and xylanase 50U) treatment was very efficient in 1% substrate (50mg). Also, glucose and xylose conversion rate of pretreated waste wood by GC analysis were 45.9% and 38.7%, respectively.
표고 및 구름버섯으로부터 Mn - peroxidase 와 Laccase 의 생산
김윤수,한옥수,배현종,고홍범 한국목재공학회 1993 목재공학 Vol.21 No.3
This study was undertaken to investigate the characteristic, and the productivities of lignin olytic enzymes: lactase (Lac) and Mn-dependent peroxidase(MnP) from Coriolus versicolor and Lentinus edodes respectively. Enzymes were isolated from cultural filterates and purified according to the standard methods. These enzymes showed one band in SDS-PAGE and their molecular weights were found 62,000 and 45,000 dalton respectively. Polyclonal antibodies against Lac and MnP were raised against mouse, In the ELISA (enzyne-lurked immunosorbent assay). Lac and MnP-antiserum produced a strong positive reaction with Lac and MnP antigen (S_(405)=2.50 and 3.53 respectively). The sera to negative (S/N)ratio way determined by the dividing the mean absorbance of antibodies by the corresponding diluted samples from normal mouse serum. The sera produced showed 2 times more positive reaction in S/N ratio than negative sera.
Laccase 와 Mn - peroxidase 에 의한 Ligninsulfonate 의 분해 특성
김윤수,문성필,배현종 한국목재공학회 1995 목재공학 Vol.23 No.4
To understand whether ligninolytic enzyme catalyze polymerization or depolymerization of the high molecular weight (HMW) lignin, the action of laccase and Mn-peroxidase (MnP) towards commercial ligninsulfonates (LS) was examined in various conditions of pH and cosubstrates. Polymerization occurred when LS was incubated with lactase at pH 6. 0. In contrast, the high molecular weight portions were significantly reduced at pH 4.5, especially when glucose was added. When LS was treated with MnP at pH 4.5, compounds of low molecular weight were produced. In particular, when cellobiose was added to Mn-P reaction mixture, the breakdown of LS was observed. In conclusion, degradation of LS by lactase and MnP occurred primarily at pH 4. 5 where as polymerization of LS was dominant at pH 6. 0. Color index, however, was not greatly changed in the degradation mixtures of LS.