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한국 약용식물부터 폴리폐놀류군의 분리 및 기능성화장품소제로서의 응용
배두경,김성기,고대원,이창언,이진태,안봉전 경산대학교 생명자원개발연구소 2001 생명자원과 산업 Vol.5 No.-
For develope the functional cosmetic material. we extracted the Korean persimmon leaves in 80% ethanol solution and isolated fraction I, II, III, to use Sephadex LH-20 column. Their polyphenol quantity was 20%, 70%, 82.8% individually and fraction II, III had polyphenol which included a many flavan-3-ol skeleton structure. We investigated various biological activity such a tyrosinase, xanthin oxidase inhibition, SOD-like acivity and DPPH. Inhibitory effect of tyrosinase was inhibited 51.7%, 74.7% at 50 ppm, 500 ppm of the fraction III and SOD-like activity increased quickly at 500ppm of the fraction II, III. DPPH effect had 80% electron donate ability at 50 ppm all of the fraction and xanthin oxidase was more inhibited 80% at 50 ppm of fraction II, III. These results showed possibility that polyphenols of the Korean persimmon leaves become the functional cosmetic ingredient
한국 재래 간장에서 분리한 Scopulariopsis brevicaulis가 생성하는 ${\alpha}-galactosidase$의 특성 및 작용양상
최광수,이선호,홍승표,이희덕,배두경,최청,Choi, Kwang-Soo,Lee, Seon-Ho,Hong, Seung-Pyo,Lee, Hee-Duck,Bae, Du-Kyung,Choi, Cheong 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.41 No.7
한국 재래 간장으로부터 분리한 Scopulariopsis brevicaulis가 생성하는 ${\alpha}-galactosidase$의 최적 생산조건은 tryptone 1.5%, $NH_4NO_3$ 0.2%, raffinose 2.5%, $KH_2PO_4$ 0.5%, yeast extract 0.5%, pH 7.0, $27^{\circ}C$에서 3일간 배양 했을 때였다. ${\alpha}-galactosidase$의 최적 pH는 7.0, 최적온도는 $27^{\circ}C$였으며, $pH\;6.0{\sim}8.0$범위와 $40^{\circ}C$이하에서 안정하였다. $Ag^{2+},\;Hg^{2+},\;Cu^{2+}$ 등의 금속이온 및 p-chloromercuribenzoic acid, Iodine에 의해 강하게 저해되어 본 효소의 catalytic부위에 -SH기가 있음이 추정되었다. Km값은 1.9 mM, Vmax값은 $9.66{\times}10^2\;{\mu}M/min$이었다. 배양기간에 따른 raffinose의 분해양상을 high performance liquid chromatography로 살펴본 결과 배양초기에 raffinose가 분해되어 sucrose, glucose, fructose가 관찰되었다. 배양기간이 경과함에 따라 raffinose가 감소하였고 sucrose, glucose, fructose도 소실됨을 확인하였다. The optimum culture condition of Scopulariopsis brevicaulis for the production of ${\alpha}$-galactosidase was as follows: Tryptone 1.5%, $NH_4NO_3$ 0.2%, Raffinose 2.5%, $KH_2PO_4$ 0.5%, yeast extract 0.5%, pH 7.0, $27^{\circ}C$. The optimum pH and temperature for the enzyme activity of ${\alpha}$-galactosidase producing Scopulariopsis brevicaulis were pH 7.0 and $27^{\circ}C$, respectively. The enzyme was relatively stable at $pH\;6.0{\sim}8.0$ and at temperature below $40^{\circ}C$. The activity of the enzyme was inhibited by $Ag^{2+},\;Hg^{2+},\;Cu^{2+}$, p-chloromercuribenzoic acid and Iodine. These results would indicate the presence of -SH groups in the catalytic site of the enzyme. Km value was 1.9 mM for $p-nitrophenyl-{\alpha}-D-galactopyranoside$ and Vmax value was $9.66{\times}10^2\;{\mu}M/min$. Sugar constituents of culture broth were identified by HPLC that the enzyme liberated sucrose, glucose and fructose from raffinose and raffinose was significantly decreased.
Bacillus coagulans CE - 74 에 의해 생산된 Biopolymer 의 생리적 기능성
안봉전,최청,김성,이선호,손준호,최희진,배두경,배종호 한국농화학회 2001 Applied Biological Chemistry (Appl Biol Chem) Vol.44 No.3
In order to prove physiological function of biopolymer from Bacillus coagulans CE-74, in vitro experiments simulating the passive membrane transport of gastrointestinal tract were carried out using dialysis membrane. And inhibition effect of isolated biopolymer on tyrosinase and angiotensin converting enzyme (ACE) were observed. The glucose retardation index after 30 min dialysis was 43.5% in the presence of 2% biopolymer. As the dialysis period became longer, the retarding effect toward glucose absorption decreased and the effect was close to zero after 5 hr dialysis. The bile acid retardation index after 30 min dialysis was 34% and 44.2% in the presence of 1% and 2% biopolymer, respectively. The effect decreased as the dialysis time elapsed. It was measured by tyrosinase inhibition activity of biopolymer that inhibition effect was 48.5% in 20 ㎍/㎕. In a ACE inhibition activity, biopolymer showed inhibition activity as 97% in 10 ㎍/㎕.
반응표면분석법을 이용한 감잎(Diospyros kaki L . folium) 에탄올 추출물의 최적화
안봉전,최청,배만종,손준호,최희진,박무희,배두경,배종호 한국농화학회 2000 Applied Biological Chemistry (Appl Biol Chem) Vol.43 No.3
The efforts were made to optimite ethanol extraction from persimmon leaf with the time of extraction(1.5∼2.5 hrs), the temperature of extraction(70∼90℃), and the concentration of ethanol(0∼40%) as three primary variables together with several functional characteristics of persimmon leaf as reaction variables. The conditions of extraction was best fitted by using response surface methodology through the center synthesis plan, and the optimal conditions of extraction were established. The contents of soluble solid and soluble tannin went up as the concentration of ethanol went up and the temperature of extraction went down, and the turbidity went down as the concentration of ethanol went down. Electron donation ability was hardly affected by the extraction temperature and had the tendency to go up as the concentration of ethanol went up. The inhibitory activity of xanthine oxidase(XOase) had the tendency to go up as both the concentration of ethanol and the temperature of extraction went up. The inhibitory activity of angiotensin converting enzyme(ACE), the significance of which still was not recognized, showed the maximum when the concentration of ethanol was 27%. In result, the optimal conditions of extraction was the extraction time of two hours, the extraction temperature of 75∼81℃, and the ethanol concentration of 33∼35%.
한국 재래 간장에서 분리한 Scopulariopsis brevicaulis 가 생성하는 α-galactosidase 의 특성 및 작용양상
이희덕,홍승표,최광수,이선호,최청,배두경 한국농화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.41 No.7
The optimum culture condition of Scopulariopsis brevicaulis for the production of α-galactosidase was as follows: Tryptone 1.5%, NH₄NO₃ 0.2%, Raffinose 2.5%, KH₂PO₄ 0.5%, yeast extract 0.5%, pH 7.0, 27℃. The optimum pH and temperature for the enzyme activity of α-galactosidase producing Scopulariopsis brevicaulis were pH 7.0 and 27℃, respectively. The enzyme was relatively stable at pH 6.0∼8.0 and at temperature below 40℃. The activity of the enzyme was inhibited by Ag^(2+), Hg^(2+), Cu^(2+), ρ-chloromercuribenzoic acid and Iodine. These results would indicate the presence of -SH groups in the catalytic site of the enzyme. Km value was 1.9 mM for ρ-nitrophenyl-α-D-galactopyranoside and Vmax value was 9.66 × 10² μM/min. Sugar constituents of culture broth were identified by HPLC that the enzyme liberated sucrose, glucose and fructose from raffinose and raffinose was significantly decreased.
감잎(Diospyros kaki folium) 으로부터 분리한 Polyphenol 화합물의 카드뮴 제거효과
안봉전,최청,배만종,손준호,최희진,박무희,우희섭,배두경,조국영 한국농화학회 2000 Applied Biological Chemistry (Appl Biol Chem) Vol.43 No.3
In order to investigate the removal effect of cadmium by polyphenol compound extracted from persimmon leaves(Diospyros kaki folium), animal test was done. Two fractions such as F-1 and F-2 from persimmon leaves were compared with their safety and function. The food intake of group F-1 and F-2 considerably decreased within 1% level. Cadmium addition influenced to rat growth(a tested animal), but food efficiency ratio(FER) wasn't shown any considerable difference in F-1 and F-2, as compared to the control. Cadmium content of liver, kidney and femur considerably decreased within 1% level in F-1 and F-2, compared to the control, cadmium content of liver decreased 25% in F-1, 28% in F-2 also decreased 22% and 25% in kidney. In femur, also decreased 53% in F-1 and 59% in F-2 respectively. The test of cadmium content in feces indicate that the content considerably increased within 1% level in both group F-1 and F-2, as compared to the control(42% in F-1, 54% in F-2). As shown the above results, have seen the removal effect of cadmium by polyphenol compound.
감나무 잎(Diospyros kaki folium) 으로부터 분리한 Polyphenol 화합물이 카드뮴 중독 흰쥐의 피해경감 효과에 미치는 영향
안봉전,최청,배만종,손준호,최희진,우희섭,배두경,조국영 한국농화학회 1999 Applied Biological Chemistry (Appl Biol Chem) Vol.42 No.4
In order to inspect safety and function regarding the reduction of cadmium poisoning by polyphenol compounds prepared from persimmon leaves (Diospyros kaki folium), animal test was done. Rats, group P-1 and P-2, were treated with cadmium and polyphenols of persimmon leaves. A control was just treated with cadmium. GOT activities in P-1 and in P-2 were lower than that of the control without considerable difference, whereas GPT activities decreased in P-1 and P-2. The SOD activity of liver tissue increased in P-l and P-2 compared with that of the control. GST activity was higher than that of the control without considerable difference in P-1; however, it increased in P-2. Lipid peroxide value considerably increased in both P-1 and P-2.