β-estrdiol dipropionate [1,3,5(10)-Estratriene-3,17 β-diol dipropionate]를 이용한 Guppy(Poecilia reticulata)의 性轉換에 관한 연구 : 生産物이 높은 實用畜의 生産에 關한 기초 연구

朴弘陽,尹鍾萬 건국대학교 1987 새마을연구논문집 Vol.6 No.-

Guppy fries were treated for the first 40 days of life with 0, 20, 40, 60 & 100㎍ of estradiol per gram of food in order to change the sex of normal undifferentiated fries to functional females. The present investigation deals with the effects of steroid hormones on the sex differentiation in guppy. Steroid hormones used was β-estradiol. Oral administration of steroid hormones caused sex reversal. The degree of sex reverse effect was dependent on the dosage level of steroid hormone. It may be assumed that the success to the higher dosage levels of steroid hormones used in the present investigation. Mild lordosis, however, was caused by the administration of the higher dosage levels of steroid hormone(60, 100㎍/g diet).

독일의 종돈 개량 방법

박홍양,Park, Hong-Yang 대한양돈협회 1986 養豚 Vol.8 No.6

독일의 가금육종에 대하여

박홍양 대한양계협회 1986 월간 양계 Vol.18 No.9

송어의 能力檢定을 위한 半循環 濾過裝置의 開發 : 農家 所得의 增大를 爲한 淡水魚 集約 飼育裝置

朴弘陽 건국대학교 1985 새마을연구논문집 Vol.4 No.-

An intensive rearing system was developed for the corntrolled breeding of cold water fish under defined environmental conditions. The system consists of rearing and a water regeneration unit has 35 single PVC-cylinder with a water volume of 801 each. This enables the separated rearing of about 150 rainbow trout of different origin from fry (about 2g) up to fingerling size (20~25g) in each cylinder. The final fish: water ratio was I : 18.6(or 150kg fish per 2.8m3 water). Water temperature is almost constant (10±2℃) all the year round. Water inflow of about 10l/min. in the cylinder cause a water exchange of seven times per hour. The addition of about 10 percent fresh water per cycle is necessary to secure a good water quality. The concentration of ammonia, nitrite, and nitrate, the pH and conductivity were measured weekly, the COD several times during the experiment. The values of PH (7.2) and of COD(2-6mg O2/1) remained rather concentration of ammonia, nitrite and nitrate increased continuously up to 0.6mg NO2-/l and 15mg No3-/l.

양어업의 현황과 기술동향

박홍양 한국축산학회 1993 축산기술과 산업 Vol.1 No.1

무지개 송어의 유전(遺傳)육종학적(育種學的)연구 : VI . 번식주기(繁殖週期)에 따른 난모세포(卵母細胞)의 발달단계 VI . Developmental Stages of Oocytes in Reproductive Cycles

박홍양,윤종만,김계웅 한국어류학회 1991 韓國魚類學會誌 Vol.3 No.2

1989年 3月부터 1990年 2月까지 채취된 무지개 松魚(Oncorhynchus mykiss) 卵母細胞의 發達 段階를 理解하기 위하여 光學 및 電子顯微鏡을 通해서 本 硏究를 實施하였다. 建國 大學校 畜産大學 養魚場에서 自然 採光 狀態에서 펠렛트 飼料를 給與하여 飼育中인 700-1,200 g의 한腹 새끼인 무지개 松魚 암컷 120마리를 供試魚로 利用하였다. 卵母細胞가 成長함에 따라 核仁의 크기가 增加하였고, 卵黃顆粒도 增加를 나타내었으며, 水化가 이루어짐에 따라 表層胞의 數가 서서히 增加하였다. 卵黃顆粒의 最大値는 卵母細胞 1個當 약 5,000個로 나타났고, 이러한 數値는 水化直前에 약 ½로 그 數가 減少하였으며, 이 時期에 核仁의 數도 有意性 있게 減少하였다. 卵黃은 顆粒狀으로 卵母細胞內에 蓄積되고, 그 格子狀은 水化가 이루어지어 卵子가 透明해지는 時期에 崩壞된다. 커다란 early maturing oocytes와 late maturing oocytes가 存在함으로써 卵巢는 增大되고, 透明하며, 顆粒狀態가 된다. 이러한 成熟狀態의 段階를 가진 무지개 松魚의 數는 9월과 10월 사이에 增加하기 때문에 平均 GSI의 數値도 增加하게 된다. GSI의 變化는 正常的인 光週期를 받는 個體와 빛이 遮斷된 個體 모두 産卵時期인 1月에 각각 14.616%과 15.532%로 最大値를 보였으며, 빛이 遮斷된 個體에서 全般的으로 높은 傾向을 나타내었다. 成熟段階(Stage VI)에 있는 무지개 松魚는 1月과 2月에 存在하며, 平均 GSI는 모든 무지개 松魚가 成熟段階에 있기 때문에 1月에 14.616%로서 가장 높은 數値를 나타내었다. 過熟段階(Stage VII)에 있는 무지개 松魚는 2月末경에 나타나며, 이러한 形態의 卵巢를 가진 무지개 松魚의 GSI는 급격하게 낮아졌다. 季節別 腦下垂體의 重量은 가을과 겨울에 有意하게(P<0.01) 무거운 變化를 보였고, 腦下垂體의 組織的 變化는 産卵時期에는 好酸性으로 granular cell이 增加하였으나, 非産卵時期에는 好鹽基性으로 vesicular cell 數가 더 많았다. 뇌하수체는 가을철인 9월경에 많은 數의 分泌顆粒(secretory globules, SG), 커다란 顆粒(globules, GL), 특히 불규칙적인 顆粒 덩어리 (irregular masses, IM) 等의 미세구조적 변화가 나타났다. 궁극적으로 卵巢의 顯微鏡的 微細形態의 季節的 變化는 外形的인 形態의 變化와 密接한 關係가 있고, GSI는 性腺의 性成熟을 測定하는데 有用한 指標로서 提示할 수 있다. This study was carried out to investigate the histological changes of ovary, pituitary gland and liver, for 3-year-old female rainbow trout (Oncorhynchus mykiss). All data were collected and analysed monthly from March 1989 to February 1990. The size of the nucleoli increased and the yolk granules increased as the oocyte grew. The number of cortical alveoli increased steadily towards hydration. The maximal number of yolk granules was nearly 5,000 per oocyte. This number was reduced to less than the half prior to hydration. A significant reduction occurred also in the number of nucleoli at that time. Yolk was deposited in the oocyte as crystalline granules. The lattice was broken down at hydration, leaving the egg transparent. Due to the presence of large early and late maturing oocytes, their ovaries were enlarged, transparent and granular. As the percentages of fish in this maturity stage increased from September to October, mean values of gonadosomatic index(GSI) increased. The GSI in fishes under normal light and under no-light in spawning period during January were the maximum of 14.616 and 15.532, respectively. Ripe fish (Stage VI) were present from January through February. Mean GSI was highest in May as all fish examined were either still maturing or ripe. The GSI increased abruptly from November to January, and showed the highest peak (14.616% in January, coinciding with the numerical drastic increase of mature oocytes in female. The weight of pituitary gland increased significantly in autumn and winter (P<0.01). Increased number of granular cells with acidophils was detected from the tissues of pituitary gland in spawning period, but in no-spawing period vesicular cells with basophils were detected. The GSI values employed in this study proved to be useful and sensitive parameters in monitoring gonad maturation. Seasonal changes in the microscopic appearance of the ovaries were well correlated with seasonal changes in both gonadosomatic index and macroscopic appearance. The ultrastructures of the gonadotrophs largely parallel the periodical changes in the ovary. Under the natural conditions, the pituitary gonadotrophs show the histological development and periodical secretion of the hormones, sufficient for a oogenesis and gonadal steroid production.

LHRH-a와 pimozide에 의한 동자개의 배란유도

박홍양,이종영,이영직,권혁추 한국양식학회 1998 韓國養殖學會誌 Vol.11 No.2

동자개의 배란유도에 대한 LHRH-a, pimozide 및 HCG의 복강내 투여효과에 대해 조사하였다. 어체중 ㎏당 HCG를 각각 5,000, 10,000, 20,000 IU를 주사하였을 경우 71.4, 80, 100%의 배란유도율을 나타냈다. 또한 pimozide의 존재에 관계없이 LHRH-a를 주사한 개체들 대부분이 배란되었다. LHRH-a 단독투여 실험에서 ㎏당 200, 300, 400 ㎍처리에서 각각 50, 75 및 100%의 배란유도율을 나타냈다. 즉, LHRH-a와 pimozide의 혼합투여에서 어체중 ㎏당 pimozide 1㎎과 LHRH-a 300㎍에서 100% 가장 좋은 배란유도율을 나타내었다. 그러나 pimpzide 단독투여에서는 배란이 유도되지 않았다. 호르몬 처리후의 생식소숙도지수(GSI)와 pseudo-GSI의 변화를 조사한 결과, HCG, LHRH-a 및 LHRH-a + pimozide를 주사한 그룹간의 생식소숙도지수(GSI)와 pseudo-GSI에 있어서 통계적인 유의차는 없었다.(p>0.05) 호르몬 처리후 배란이 일어나기까지의 소요시간은 HCG 처리후 17∼22시간, LHRH-a 단독 또는 pimozide와의 혼합처리에서 대체로 23∼29시간이 걸렸다. Effects of intraperitoneal administration of LHRH-a (luteinizing hormone releasing hormone analogue), pimozide and HCG (human chorionic gonadotropin) were studied on ovulation in the bullhead, Pseudobagrus. fulvidraco. Ovulation was induced in 71.4, 80 and 100% by injection of HCG 5,000, 10,000 and 20,000 IU/㎏ of body weight, respectively. Also, the majority of fish injected with LHRH-a in the presence or absence of pimozide were ovulated. The ovulating rates in fish injected with LHRH-a alone showed 50, 75 and 100% at a dose 200, 300 and 400 ㎍/㎏, respectively. Pimozide enhanced the activity of LHRH-a, the most effective combination for inducing ovulation (100%) was LHRH-a at 300 ㎍/㎏ plus pimozide at 1 ㎎/㎏. Pimozide alone was ineffective in inducing ovulation of P. fulvidraco. There were no significant differences in gonadosomatic index (GSI) and pseudo-GSI values among the groups treated with HCG, LHRH-a and LHRH-a plus pimozide (p>0.05). The latency time of group treated with HCG was 17∼22 hours, and the group injected with LHRHa alone and/or in combination with pimozide was ovulated within 23∼29 hours.

특집 4 - 건국유업.건국햄의 과거, 현재, 그리고 미래

박홍양,Park, Hong-Yang 한국대학교육협의회 2009 大學敎育 Vol.160 No.-

서독의 계통조성과 실용축 생산을 위한 교배체계

박홍양,Park, Hong-Yang 대한양돈협회 1987 養豚 Vol.9 No.10

육종-독일의 실용축 생산 프로그램에 관하여 (II)

박홍양,Park, Hong-Yang 대한양돈협회 1986 養豚 Vol.8 No.10