섬유소 당화현탁액에 함유된 Spent Cellulase의 탈착, 회수 및 재이용

박진서,이용현 한국산업미생물학회 1990 한국미생물·생명공학회지 Vol.18 No.2

섬유소 효소당화 후 미분해 잔류 섬유소에 흡착된 spent cellulase와 당화액에 함유된 미흡착 spent cellulase를 최대한 회수 재사용코져 연구하였다. 반응초기에는 soluble protein과 효소활성의 대부분이 흡착된 상태에 있었으며, 특히 FPase가 CMCase보다 더욱 강하게 흡착되어 있었다. 24시간 당화 후 soluble protein은 53%인 4.77g/l, CMCase activity는 40%인 10,040IU/l, 그리고 FPase activity는 55%인 6,633IU/l가 미분해 잔류섬유소에 흡착되어 있었다. 미분해 잔류섬유소에 흡착되어 있는 spent cellulase의 재회수를 목적으로 pH 및 온도의 변화, chemical agents의 첨가에 따른 회수율을 검토하였다. 미분해 잔류섬유소에 흡착되어 있는 spent cellulase의 최족회수조건은 50℃에서 0.1% Tween 80을 함유한 pH 10인 glycine-NaOH buffer 용액으로 처리하였을 경우였으며, 이 때 흡착되어 있는 soluble protein의 회수량은 62.2%인 2.99g/l, CMCase activity는 62%인 6,225IU/l, FPase activity는 44%인 2,919IU/l였다. Triton X-100, isopropanol, glycerol, ethylene glycol, silicon oil과 같은 chemical agent 첨가시에도 우수한 결과를 얻었다. 한편 가수분해액에 함유된 미흡착 spent cellulase는 fresh substrate에의 흡착 후 filtration하는 방법, acetone precipitation법, 그리고 ultrafiltration법으로 회수하였다. 특히 fresh substrate에 흡착 후 filtration하는 방법이나 ultrafiltration법을 이용할 경우 높은 회수율을 얻을 수 있었다. 위의 최적조건에서 흡착된 효소를 탈착시키고 탈착된 효소와 당화액 중에 남아있는 spent cellulase를 기질 흡착성을 이용하여 fresh substrate에 재흡착시킨 결과 soluble protein 70%, CMCase activity 65%, FPase activity 57%가 최종적으로 회수되었다. To enhance the process economics for enzymatic hydrolysis of cellulose, the recovery and reuse of spent cellulase is essential because of high cost of enzyme. The various desorption methods for recovery of adsorbed spent cellulase on undigested cellulose were compared. The maximum desorption, with recovery yield of 62.2% of soluble protein, 62% of carboxymethyl cellulase(CMCase ) activity, and 44% of filter paper(FPase ) activity, was achieved by treating with pH 10 glycine-NaOH solution at 50℃ in the presence of 0.1% Tween 80. On the other hand, around 87% of the free spent cellulase contained in hydrolysate was successively recovered by adsorption on fresh substrate and then filtration with sinter glass. Total recrvery yield of spent cellulase, around 70% of soluble protein, 57% of filter paper (FPase) activity, and 65% of carboxymethyl cellulase(CMCase) activity, was achieved by desorption and readsorption on fresh substrate.

항공분야 온실가스 배출량 산정방법 정립을 위한 정책적 제언

박진서,김제철,최성원 한국교통연구원 2010 交通硏究 Vol.17 No.3

전 세계적으로 항공부문의 에너지 소비량은 매년 증가하고 있는 가운데 EU의 항공부문 배출권 거래제 도입과 ICAO의 온실가스 저감대책 추진 등 배출가스 규제가 본격적으로 진행되고 있다. 이는 우리나라의 항공산업에 직간접적인 영향을 미칠 것으로 예상되고 있다. 이와 같은 온실가스 규제정책에 적극적으로 대응하기 위해서는 국가 온실가스의 체계적인 관리와 더불어 배출량 산정방법 정립이 선행되어야 한다. 본 연구는 항공분야 배출가스 산정에 있어 그간 선행연구의 실제 배출량이 상이한 내용에 대하여 문제점을 제기하고 타당한 산정방법론을 정립하여 그에 따른 정책적 제언을 마련하는 것이 목적이다. 이를 위해 기후변화정부간위원회(IPCC, Intergovernmental Panel on Climate Change, IPCC)의 배출가스 산정 가이드라인에 따라 항공부문의 선행연구를 비교분석하여 배출량 결정요인과 방법론을 정립하였다. 또한 정립된 산정방법을 기초로 하여 2005년부터 2009년까지 우리나라 항공부문 연도별 온실가스 배출량을 산정하였으며, 항공부문의 온실가스 인벤토리 구축을 위한 정책적 제언을 제시하였다. Energy consumption of aviation sector is increasing in the whole world. Thus, introduction to ETS in aviation sector of EU and greenhouse gases reduction plans of ICAO proceed such as restriction of emission gas. It is expected to that has an direct and indirect influence on aviation industry in Korea. To cope with greenhouse gases regulation proactively, it needs for effective management of greenhouse gases and take triangular position of estimation method for quantity of emissions. The purpose of this research is to provide advice by building estimation method. Also this study bring forward problems about different contents in actual quantity of emissions in the advance study for the calculating emission gases in aviation sector. According to emission gas calculating guidelines of Intergovernmental Panel on Climate Change, determinant of emissions and methodology have been built with comparison analysis from the advance study in aviation sector. Also, based on built calculation method, it calculated quantity of greenhouse gases every year from 2005 to 2009 on aviation sector in Korea. Furthermore, this study come up with advice for policy to build emission gas inventory in aviation sector.

커리어 쇼크에 대비하자

朴晉緖(박진서) 관훈클럽 2002 관훈저널 Vol.43 No.1

온몸의 연속절단면영상 만들기, 제7보고 : 다리의 구조물을 낱낱이 구역화하기

박진서,황성배,정민석 대한해부학회 2006 Anatomy & Cell Biology Vol.39 No.4

A Proposal of New Reference System for the Standard Axial, Sagittal, Coronal Planes of Brain Based on the Serially-Sectioned Images

박진서,정민석,박효석,신동선,하동환,조장희,김영보,한재용,지제근 대한의학회 2010 Journal of Korean medical science Vol.25 No.1

Sectional anatomy of human brain is useful to examine the diseased brain as well as normal brain. However, intracerebral reference points for the axial, sagittal, and coronal planes of brain have not been standardized in anatomical sections or radiological images. We made 2,343 serially-sectioned images of a cadaver head with 0.1 mm intervals, 0.1 mm pixel size, and 48 bit color and obtained axial, sagittal, and coronal images based on the proposed reference system. This reference system consists of one principal reference point and two ancillary reference points. The two ancillary reference points are the anterior commissure and the posterior commissure. And the principal reference point is the midpoint of two ancillary reference points. It resides in the center of whole brain. From the principal reference point, Cartesian coordinate of x, y, z could be made to be the standard axial, sagittal, and coronal planes.

Segmentation of Cerebral Gyri in the Sectioned Images by Referring to Volume Model

박진서,정민석,지제근,박효석,신동선 대한의학회 2010 Journal of Korean medical science Vol.25 No.12

Authors had prepared the high-quality sectioned images of a cadaver head. For the delineation of each cerebral gyrus, three-dimensional model of the same brain was required. The purpose of this study was to develop the segmentation protocol of cerebral gyri by referring to the three-dimensional model on the personal computer. From the 114sectioned images (intervals, 1 mm), a cerebral hemisphere was outlined. On MRIcro software, sectioned images including only the cerebral hemisphere were volume reconstructed. The volume model was rotated to capture the lateral, medial, superior, and inferior views of the cerebral hemisphere. On these four views, areas of 33 cerebral gyri were painted with colors. Derived from the painted views, the cerebral gyri in sectioned images were identified and outlined on the Photoshop to prepare segmented images. The segmented images were used for production of volume and surface models of the selected gyri. The segmentation method developed in this research is expected to be applied to other types of images, such as MRIs. Our results of the sectioned and segmented images of the cadaver brain, acquired in the present study, are hopefully utilized for medical learning tools of neuroanatomy.

Acetalization of glycerol using mesoporous WO3/rod-type SBA-15 acid catalyst

박진서,이정화,이성빈,홍경희,김지만 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.0

Acetalization of glycerol has a great industrial importance due to the large production of glycerol in biodiesel industry. The products have been widely used as fuel additives and surfactant bases. Herein, glycerol conversion with acetone has been studied using mesoporous WO3/rod-type SBA-15 as a catalyst. A series of catalyst with varying WO3 loadings (5-20wt%) were prepared by impregnation method using tungsten precursor. The catalysts were characterized with XRD, Raman spectroscopy, SEM, N2 adsorption-desorption analysis and NH3-TPD. Among the catalysts, 15wt% WO3/rod-type SBA-15 was found to be the most active at 30℃. The catalyst exhibited 99% conversion with 97% selectivity for five-membered (1,3-dioxolane) cyclic product. The excellent performance of the catalyst is mainly due to their high specific surface areas, high pore volumes and strong surface acid properties. These results suggest that WO3/rod-type SBA-15 is highly active catalyst for the acetalization of glycerol.

절단면영상으로 만드는 얼굴곡면영상

박진서 대한체질인류학회 2017 대한체질인류학회 학술대회 연제 초록 Vol.60 No.-

온몸의 연속절단면영상 만들기 (첫째 보고: 포매하고 연속절단하는 방법)

박진서,정민석,김진용,박형선 대한해부학회 2002 Anatomy & Cell Biology Vol.35 No.4

Design and Implementation of Security Function According to Routing Method in Automotive Gateway

박진서,김대현,서일홍 한국자동차공학회 2021 International journal of automotive technology Vol.22 No.1

In recent years, vehicle networks require high bandwidth due to the increasing complexity of electronic control devices for vehicles due to the demand for advanced driving aids, infotainment and V2X communication. Therefore, Ethernet protocol was introduced and vehicle gateway system was newly introduced in vehicle system. The vehicle gateway system provides an interface for continuously connecting and exchanging vehicle data in different communication environments between the Controller Area Network (CAN) protocol and the Ethernet network protocol. There are two types of data exchange in the vehicle gateway. These are direct routing and indirect routing. Vehicle gateways are connected to external networks, which can lead to security vulnerabilities. So it needs security function to ensure message integrity. Recently, vehicle manufacturers have introduced gateway systems with security features that operate to verify the integrity of messages using cipher-based message authentication codes (CMAC). But applying security functions to a gateway system introduces delays in performing security functions. Therefore, in this paper, to design stable and efficient the vehicle gateway system with security function, we have tested and evaluated the latency time that can occur according to the routing methods which are direct routing and indirect routing.