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꾸지나무 유래 화합물 Broussochalcone B의 HepG2 간암세포의 세포사멸에 미치는 영향
박진량,류형원,조병옥 한국생약학회 2017 생약학회지 Vol.48 No.4
The aim of this study was to investigate the mechanisms underlying apoptosis induced by a broussochalcone B (BCB) from Broussonetia papyrifera in HepG2 cells. The results showed that BCB treatment for 24 hr significantly inhibited cell viability in a dose-dependent manner, and induced apoptosis in HepG2 cells. More so, BCB treatment triggered the cleavage of caspase-8, -9, -3, poly (ADP-ribose) polymerase (PARP), increase of Bax level, and decrease of Bcl-2 expression. A general caspase inhibitor (z-VAD-fmk) blocked BCB-induced cell death. Furthermore, BCB treatment caused reactive oxygen species (ROS) production in a dose-dependent manner. In addition, an antioxidant N-acetylcysteine (NAC) blocked BCB-induced ROS production and cell death. Therefore, these results indicate that BCB-induced apoptosis is mediated by a caspase dependent pathway and ROS production in HepG2 cells.
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김중현,박진량,김성호,윤창영 한국자원식물학회 2011 한국자원식물학회지 Vol.24 No.5
This study was carried out to investigate the flora of Mt. Gahyeon (Gyeonggi-do, Gimpo). The vascular plant were collected 13 times (from March to Oct. 2010), and 413 taxa in total were identified, including 96 families, 267 genera, 362 species, 6 subspecies, 40 varieties and 5 forms. Among them were 6 taxa of Korean endemic plants and 17 taxa of specially designated plants by the Ministry of Environment. Usage of 413 taxa were consists of 159 taxa (38.5%) of edible plants, 122 taxa (29.5%) of medicinal plants, 77 taxa (18.6%) of pasture plants, 47 taxa (11.4%) of ornamental plants, 26 taxa (6.3%) of stainable plants, 14 taxa (3.4%) of timber plants, 6 taxa (1.5%) of fiber plants, 5 taxa (1.2%) of industrial plants and 102 taxa (24.7%) of unknown resource plants, respectively. The naturalized plants were identified as 41 taxa and the percent of naturalized index (NI) was 9.9% of total 413 taxa vascular plants. Furthermore, therophytes (27.1%), hemicryptophytes (26.4%), geophytes (14.7%) showed high proportional ratio in life form spectrum. 본 연구는 가현산의 관속식물상을 밝히고 주요 식물들의 분포를 조사하였다. 2010년 3월부터 10월까지 총 13회에 걸쳐 수행되었다. 그 결과 관속식물은 96과 267속 362종 6아종 40변종 5품종으로 총 413분류군이 확인되었다. 그 중 한국 고유종은 6분류군, 식물구계학적 특정식물종은 17분류군이 조사되었다. 조사된 413분류군의 유용도는 식용이 159분류군(38.5%), 약용 122분류군(29.5%), 목초용 77분류군(18.6%), 관상용 47분류군(11.4%), 염료용 26분류군(6.3%), 목재용 14분류군(3.4%), 섬유용 6분류군(1.5%), 공업용이 5분류군(1.2%) 그리고 용도를 알지 못하는 식물도 102분류군(24.7%)으로 나타났다. 귀화식물은 41분류군으로 확인되었으며, 귀화율은 9.9%로 나타났다. 또한 생활형조성은 일년생식물(27.1%), 반지중식물(26.4%), 지중식물(14.7%)등이 높게 나타났다.
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송아지 설사분변으로부터 Shiga toxin-producing Escherichia coli 의 분리 및 특성규명
임금기,강문일,김상기,남경우,박현주,박진량,조경오,이봉주,Lim, Keum-Gi,Kang, Mun-Il,Kim, Snag-Ki,Nam, Kyung-Woo,Park, Hyun-Joo,Park, Jin- Ryang,Cho, Kyoung-Oh,Lee, Bong-Joo 대한수의학회 2006 大韓獸醫學會誌 Vol.46 No.2
Shiga toxin (stx) producing Escherichia coli (STEC) causes various clinical signs in animal and human. In this study, 255 fecal samples from calves showing diarrhea were collected from cattle farms in Chonnam province during the period from January 2005 to July 2005. Twenty six STEC (10%) were isolated from 255 fecal samples by PCR. The isolates displayed three different stx combinations (stx1 [69%], stx1 and stx2 [15%], and stx2 [38%]). The isolates were further studied for virulence associated genes and antimicrobial resistance to define the virulence properties. Intimin (eaeA), enterohemolysin (hlyA), and lipopolysaccharide (rfbE) virulence genes were detected in 6 (23%), 7 (26%), and 1 (3.8%) of the isolates, respectively, by PCR. One isolate possessing rfbE gene was typed as E. coli 0157 : H7 by agglutination test with O and H antisera. All 26 isolates showed susceptibility to amikacin (100%) and the majority of isolates showed high susceptibility to gentamicin (88.5%) and chloramphenicol (73.1%). But all isolates were resistant to penicillin. These results may provide the basic knowledge to establish strategies for the treatment and prevention of enteric disease in calves.
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