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박준봉,이기대 慶北大學校 齒科大學 1988 慶北齒大論文集 Vol.5 No.1
To obtain the basic knowledge of methods for biological evaluation of every dental materials and drugs used in dentistry author have used Vero cell (African green monkey kidney cell) to investigate of cytotoxic effects of cholorhexidine gluconate which used for oral gargling solution. Eagle's Minimum Essential Medium with 5% Fetal Bovines Serum were used in this study. After seeding the same number of cell to each 60㎜ Petri dish with medium including different concentration of chlorhexidine, all of the dishes were incubated in the 95% Co_2, 5% O_2 incubator. The number of cells in the each dishes were counted on the hemocytometer after 24, 48 hour incubation. The following results were obtained: 1. The number of survival cells was depend on the concentration of chlorhexidine in medium. The higher of concentration of chlorhexidine showed more decreasing the number of the each dish. 2. In the case of medium only used, the number of cells was increased two times every hour after seeding. 3. In the case of 0.001% chlorhexidine medium used, there was no change of number of cells during the 48 hour.
朴準奉,申洪仁 慶北大學校 齒科大學 1986 慶北齒大論文集 Vol.3 No.1
The purpose of this study is to determine the pattern of the histological changes and resorption of cyanoacrylate which have been used as plastic tissue adhesive and periodontal dressing materials. For this experimental study, 20 male mice were injected the cyanoacrylate into subcutaneous tissue of posterior femur area by syringe. The results were examined by light microscope for the histologic appearance with conventional stainable specimen. The results were as follows ; 1. Edematous tissue changes and infiltration of acute inflammatory cell were revealed on the subcutaneous tissue in early stage. 2. From the 14th days, the loss of edematous changes and dense fibrous tissue encapsulation surround the cyanoacrylate by collagenesis from granulation tissue were shown. 3. polynuclear giant cell were observed since 3 rd week, but there were not revealed resorptive phenomenon caused by them. 4. In the case of injected to muscle fiber, cyanoacrylate seperated the muscle fiber bundle. 5. During the whole period of this study, the amount of infiltration of inflammatory cell observed in the subcutaneous tissue less than in the muscle fiber.
朴準奉,李基大 慶北大學校 齒科大學 1985 慶北齒大論文集 Vol.2 No.1
To evaluate the relationship of the hardness and properties of the periodontal hand instruments, the authors investigated the wearness and surface texture of the instruments with metallurgical microscope and Knoop Hardness Tester. Four Gracey curettes and three sickle scalers were used in the study. The results were as follows; 1. The curette which had the highest hardness valus was the Gracey curette of Hu-Friedy Co. 2. The hand scaler which had the gighest hardness value was Jaquette # 1, the scaler of Hu-Friedy Co. 3. In the view of the metallurgical microscope, all periodontal hand instruments revealed the stainless steel of the Martensite system.
전기적 자극이 배양 두개관 골세포의 석회화에 미치는 영향에 관한 연구
박준봉,허인식,이혜자,최영철,Park, Joon-Bong,Hur, In-Sik,Lee, Hye-Ja,Choi, Young-Chul 대한치주과학회 1997 Journal of Periodontal & Implant Science Vol.27 No.4
To date, various clinical procedures have been used to restore periodontal apparatus destroyed by periodontal disease. And then, many experimental approaches have been proceeded to develop treatment methods to promote periodontal regeneration. Mechanical, chemical treatments to enhance the attachment of periodontal tissue cells as changing the physical properties of root surfaces, bone graft procedure, and treatments for guided tissue regeneration have been used for periodontal regeneration. However, recent studies have revealed that biologic factors such as growth factors promote biologic mechanism associated with periodontal regeneration. This study was done to enucleate how ELF stimulus affect the periodontal regeneration. We can have following conclusions from this experimental results. The influence of low frequency(ELF) electric stimulus (30Hz at $lO{\mu}A$) known to promote bone formation in vivo, was evaluated for its ability to affect bone cell function in vitro. After 12 hour exposure of ELF stimulus at most appropriate densities ($5{\times}10^4\;cells/cm^2$) to increase osteoblastic cells normally, rat calvarial cells were incubated for 60 hours were used in this study. We have found ELF stimulus suppress calvarial cell proliferation and the ability of protein synthesis, enhance the alkaline phosphatase activity significantly.