생약의 ${\beta}-Glucuronidase$ 저해와 간장보호효과

심상범,박주석,김남재,김동현,Shim, Sang-Bum,Park, Ju-Suk,Kim, Nam-Jae,Kim, Dong-Hyun 한국생약학회 1999 생약학회지 Vol.30 No.2

Inhibitory effect of water and ethyl acetate extract of 60 kinds of herbal medicines was investigated on ${\beta}-glucuronidase$. Among water extract of them, Galla Rhois had the most potent ${\beta}-glucuronidase-inhibitory\;activity$. Termaliae Fructus, Amomi Tsa-ko Fructus and Arecae Semen were also potent inhibitors. Among ethyl acetate extract of them, Galla Rhois had the most potent ${\beta}-glucuronidase-inhibitory\;activity$. Nelumbinis Semen. Ephedrae Radix and Termaliae Fructus were also potent inhibitors. The extract of Galla Rhois had potent hepatoprotective effect on $CCl_4-induced$ hepatotoxicity of rats. These results suggest that the ${\beta}-glucuronidase$ seems to be closely related to the liver injury, which could be prevented by the inhibitor of ${\beta}-glucuronidase$.

녹용발효와 생리활성

김동현,한상범,박주석,한명주,Kim, Dong-Hyun,Han, Sang-Bum,Park, Ju-Suk,Han, Myung-Joo 한국생약학회 1994 생약학회지 Vol.25 No.3

Bacillus P-92 which fermented antler was isolated from intestinal bacteria. The biological activites, carbon clearance and growth activity of lactic acid bacteria, of fermented antler was better than those of untreated antler. The enyzmes activities, ${\beta}-glucosidase,\;{\beta}-glucuronidase$ and tryptophanase, of intestinal bacteria of mice treated with fermented antler were lower than those of mice treated with untreated antler, although those of mice treated with fermented antler or untreated antler were higher than those of control. Biological activity of the antler seems to be increased by fermentation.

배드민턴 경기 승패에 따른 혈중 젖산농도 및 스트레스 호르몬 분석

김상수(Kim, Sang-Su),박성빈(Park, Sung-Bin),박영호(Park, Young-Ho),이소정(Lee, So-Jung),김진환(Kim, Jin-Hwan),윤해철(Yoon, Hae-Chul),주석범(Ju, Suk-Bum),황병준(Hwang, Byung-Jun),박찬후(Park, Chan-Hu),윤성덕(Yoon, Sung-Duk) 한국체육과학회 2013 한국체육과학회지 Vol.22 No.5

The following research was conducted in order to compare changes in blood lactate levels and stress hormones, such as cortisol, epinephrine, and norepinephrine, of twenty male university students who belonged to a badminton club and arrived at the following conclusions: 1. The changes in blood lactate levels did not show a significant difference in the subjects before or after a game, or between winning and losing groups. 2. The cortisol levels within the same group also did not show a significant change before or after the game; however, the losing group showed higher cortisol levels, and the winning group showed significantly higher levels of cortisol (p<.05). 3. There was no significant change in epinephrine levels between subjects in the same group; however, the losing group showed slightly lower epinephrine levels before the game and the winning showed slightly higher levels. There was, however, no significant difference between the two groups. 4. The change in norepinephrine levels in the winning group dropped after the game and increased in the losing group. But the change was not statistically significant. In conclusion, this research looked into the blood lactate and stress hormone levels of university badminton club students with less than one year of experience. According to the research, blood cortisol levels of the losing group was significantly higher than that of the winning group. The epinephrine and norepinephrine levels of the winning group"s participants dropped by a statistically insignificant amount after a game. These results are in contrast to existing research results. This can be attributed to anxiety factors before games and achievement factors such as winning. In order to better explain the changes in stress hormone levels due to winning or losing, further psychological and physiological research on various sporting mediums and skill levels need to be conducted.

대장암모델 생쥐에 발효녹용의 발암 억제 효과

김동현(Dong Hyun Kim),이승원(Seung Won Lee),박주석(Ju Suk Park),장일성(Il Sung Jang) 경희대학교 동서약학연구소 1995 경희약대 논문집 Vol.23 No.-

N/A

5 . 18 광주민주화운동 관련 기록사진의 발굴 및 분석

박주석,조대연 한국사진학회 2001 AURA Vol.8 No.1

It could be seen through this research that the formal photo records were very few and feeble for 5.18 Kwangju Democratic Movement in 1980 considering the historicity and the great influential power which the movement itself possessed. Particularly, as for the photo records of domestic newspapers which stood at the point of an advance guard, the photo record itself and report were extremely few though there were periodic limitations. If the newspapers at that time had recorded and reported the movement thoroughly with photos, the problems of Kwangju would not have ended simply as the problem of Kwangju itself. Moreover, the way which could get access to disclosing the reality could have been opened. Compared with this fact, the efforts of the partial photo reporters at the front line who tried their best to be faithful to keep the periodic records should be highly appreciated. Though their photos could not be shown in the media of those days, the fact that they could make it easy to get access to the fact of 5.18 Kwangju Democratic Movement in 1980 later through photos was very important. They were very faithful to their inherent duty of recording the fact through records at the circumstance which bore a close resemblance to the actual warfare. It can be said that they again proved the attribute of verifying the fact which photos possessed by means of their photo records. On the other hand, as seen in the frame of photo analysis of <If The Day of May Comes Again> or photos by the reporter, Na Gyeong-taeg, the ongoing process and problems of 5.18 Kwangju Democratic Movement in 1980 could be seen. Furthermore, the fact that Kwangju citizens were not the mob but the people who had resistance spirits which had earnest desire for democratization and mature citizen consciousness could be recognized through the analysis of photos themselves. In addition, the efforts of the social organization such as <Justice-Peace Committee of Kwangju Head-Parish of Catholic Church> which gathered photo records at that time and actively utilized them for disclosing the real situation and for the movement to publicize the actualities of the struggle should be highly appreciated. Particularly, 225 photos, which were printed in the <If The Day of May Comes>, including 14 photos which were put in <Schuern>, which was the German weekly News magazine, and 75 photos by anonymous citizens, and 153 record-photos by Na Gyeong-taek were the photo records which could reveal the actual substance of 5.18 Kwangju Democratic Movement in 1980 photographically. And, besides, photos of the foreign press such as AP and UPI can be considered as important records which should be accessed, if possible. Photos have been positively considered as the best media which can reach the reality of the history and the fact through the role of faithful record of the fact from the very time they were invented. Man had made continuous efforts to develop the value of such photos. We could recognize the importance of the photo records once again through this fact. Likewise, we also came to realize the fact that photographers must do their best for accomplishing their social responsibilities to keep photo records with a true heart by all means.

내분비계 교란물질의 검출계를 이용한 γ-HCH의 미생물에 의한 중간대사산물에 대한 내분비계교란 활성의 평가

이행석,박주석,조은민,문명숙,太田明德,류재천 대한상하수도학회 2003 상하수도학회지 Vol.17 No.1

To develop an efficient degradation system for Endocrine disruptors (EDs), it is necessary to have a good system to evaluate rapidly and accurately endocrine-disrupting activities of suspected chemicals and their degradation products. We previously constructed a co-expression system of GAL4 DNA binding domain (DBD)-human estrogen receptorβ ligand binding domain (hERβ LBD) and Gal4p transcriptional activation domain (TAD)-co-activator SRC1 in Saccharomyces cerevisiae with a chromosome-integrated lacZ reporter gene under the control of CYC1 promoter and GAL4 binding site (Upstream Activating Sequence, UAS). Expression of this reporter gene was dependent on the presence of estrogen or endocrine disruptors in the culture medium. Furthermore, the extent of transcriptional activation by those chemicals correlated with their estrogenic activities measured by other assay systems, indicating that this assay system is efficient and reliable for measuring estrogenic activity. We applied this assay system to measure estrogenic activity of microbial degradation products of γ-hexachlorocyclohexane (γ-HCH) by Sphingomonas paucimobilis. Among the γ-HCH metabolites, 2,5-dichlorohydroquinone (2,5-DCHQ) and chlorohydroquinone (CHQ) had similar estrogenic activities to the original chemical, but hydroquinone (HQ), a metabolite at later stage, had no activity at the concentration of 10^-4M, showing the necessity of evaluation of intermediate metabolites in microbial degradation systems.

생약의 β-Glucuronidase 저해와 간장보호효과

김남재,심상범,박주석,김동현 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 1999 東西醫學硏究所 論文集 Vol.1999 No.-

Inhibitory effect of water and ethyl acetate extract of 60 kinds of herbal medicines was investigated on β-glucuronidase. Among water extract of them, Galla Rhois had the most potent β-glucuronidase-inhibitory activity. Termaliae Fructus, Amomi Tsa-ko Fructus and Arecae Semen were also potent inhibitors. Among ethyl acetate extract of them, Galla Bhois had the most potent β-glu-curonidase-inhibitory activity. Nelumbinis Semen. Ephedrae Radix and Termaliae Fructus were also potent inhibitors. The extract of Galla Rhois had potent hepa-toprotective effect on CCl₄-induced hepatotoxicity of rats. These results suggest that the β-glucuronidase seems to be closely related to the liver injury, which could be prevented by the inhibitor of β-glucuronidase.