손상된 흰쥐 척수에 사람중간엽세포 이식시기에 따른 영향

박영란(Young Lan Park),문정석(Jeong Seok Moon),정윤영(Yoon Young Chung),김 진(Jin Kim),송창훈(Chang Hun Song),김종중(Jong Joong Kim) 대한해부학회 2007 Anatomy & Cell Biology Vol.40 No.2

골수와 탯줄혈액에서 분리한 중간엽세포(mesenchymal stem cells: MSCs)는 각종 질병을 치료하는데 많이 이용하는 성체줄기세포이다. 본 연구에서는 흰쥐에 척수손상(spinal cord injury: SCI) 후 꼬리 정맥에 이식한 사람중간엽세포(human MSCs: hMSCs)가 손상부위로 안정하게 이주하여 신경세포 및 신경아교세포로 분화되는지 관찰하였고 hMSCs의 이식날짜에 따른 SCI로 인한 마비된 척수의 운동기능의 회복률 차이를 비교하고자 본 실험을 시도하였다. 본 실험에서 SCI은 척추궁절제술을 시행하여 8~9번째 흉추부위척수를 노출시킨 후 동맥류 집게를 이용하여 약 30초간 압박하여 손상시켰다. 이식에 이용한 hMSCs는 세포 생존율을 측정하기 위하여 이식하기 직전에 cholera toxin subunit B (CTX-B) FITC로 미리 표지하였고 이후 hMSCs를 쥐의 꼬리정맥을 통해 이식하거나 SCI 부위에 직접이식을 하였다. 이식된 CTX-B FICT로 표지된 hMSCs가 손상된 부위에 이주하였으며, 이식된 hMSCs는 이식한 전체의 수에 비하면 매우 적었다. 그리고 SCI1+hMSCs 군에 서 관찰된 CTX-B FITC로 표지된 hMSCs은 SCI5+hMSCs 군보다 더 많이 분포하였다. human specific mitochondria항체를 이용한 면역조직화학법을 통해 CTX-B FICT로 표지된 세포가 hMSCs임을 재확인하였으며 이 중 일부는 신경세포의 표지단백질들이 발현되었다. 또한 hMSCs가 면역억제제를 쓰지 않은 조건에서도 일부세포는 4주까지 생존함을 확인할 수 있었다. 그리고 SCI후에 hMSCs를 이식한 흰쥐에 행동평가를 시행한 결과 대체적으로 뒷다리의 운동성이 서서히 회복됨을 관찰할 수 있었다. 특히 SCI을 시행하고 1일 후에 이식한 실험군(SCI1+hMSCs)이 5일 후에 이식한 실험군(SCI5+hMSCs)보다 뒷다리 운동이 더 효과적으로 회복되었다. 따라서 본 연구의 결과는 SCI후에 hMSCs의 이식은 SCI 후 흰쥐 운동성 회복에 기여할 수 있다는 것을 확인할 수 있었으며 이러한 hMSCs에 대한 연구는 자가이식, 윤리적, 면역학적 측면에서 다른 방법의 세포치료에 비해 더욱 유용한 치료방법이라 할 수 있으며 임상응용에 중요한 기초가 될 것으로 판단된다. Two sources of adult stem cells that have aroused great interest are human bone marrow-derived mesenchymal stem cells (hMSCs) and human umbilical cord blood cells. hMSCs have been reported to maintain their ability to differentiate into neuronal lineage cells in the central nervous system. Therefore, transplantation of hMSCs represents an attractive new form of cellular therapy for clinical application in spinal cord injury (SCI). The aim of this study was to investigate how transplanted hMSCs from the venous circulation moved into a target zone of compression injury in the spinal cord of rats, and if they ameliorated the behavioral impairments associated with SCI. SCI in rats was induced by compressing the spinal cord for 30 s with an aneurysm clip. hMSCs labeled with cholera toxin subunit B conjugated to fluorescein isothiocyanate (CTX B-FITC) were injected intravenously through the tail vein or directly on the SCI site using a 27-g needle. Suspensions of hMSCs collected from adult humans were delivered at concentrations (1×106cells/200 μL) in 1 or 5 d after experimental SCI. After transplantation of hMSCs, the SCI regions displayed some endogenous background fluorescence, but CTX B-FITC-labeled hMSCs were clearly identifiable. They were observed in injured but not in intact areas; they were usually round or slightly elongated with a prominent nucleus. Only a few hMSCs were found in the spinal cord in each case but there were more cells in the rats injected at day one than at day five. This study confirmed that these were indeed transplanted hMSCs using antisera recognizing human-specific nuclei or human-specific mitochondria. Double immunofluorescence analysis showed the production of some neuronal and glial cell markers in the SCI lesions. Behavioral test scores of SCI rats treated with hMSCs at day one were significantly better than those for rats treated at day five and for the untreated SCI group. Thus, hMSCs appear to be beneficial in reversing the behavioral effects of SCI in this rat model, even when infused one day after injury. They might be a viable source of stem cells for the treatment of human neurological disorders.

사회적 지지와 사회적 부정성이 농촌 노인의 우울감에 미치는 영향에 관한 연구

박영란(Park, Yeong-Ran),박경순(Park. Kyung-Soon),정은화(Jung, Eun-Hwa) 한국정신건강사회복지학회 2014 정신보건과 사회사업 Vol.42 No.2

본 연구는 농촌 노인의 사회적 관계가 정신건강에 미치는 영향을 분석함에 있어서 특히 사회적 관계의 긍정적 차원인 사회적 지지와 부정적 차원인 사회적 부정성이 농촌 노인의 우울감에 미치는 영향력의 차이를 검증하는 데 목적이 있다. 이와 더불어 사회적 지지원의 유형에 따라 사회적 지지와 사회적 부정성이 우울감에 미치는 영향의 차이를 확인하였다. 이를 위해 구조화된 설문지를 사용하여 경기도 K군 1개 면의 노인을 전수 조사한 “한국인의 사회적 삶, 건강한 노화에 대한 조사(KSHAP: Korean Social Life, Health and Aging Project)” 자료 중에서 60세 이상의 유배우자 노인 569명을 분석의 대상으로 하였다. 연구 결과에 따르면 사회적 지지와 사회적 부정성은 농촌 노인의 우울감에 유의미한 영향을 주는 것으로 나타났는데, 사회적 부정성의 영향력이 사회적 지지보다 더 크게 나타났다. 사회적 지지원의 유형별로 살펴보면 배우자의 사회적 지지와 사회적 부정성은 우울감에 유의미한 영향을 미치는 것으로 나타났으며, 친구(이웃)의 경우에는 사회적 부정성만이 우울감에 영향을 주는 것으로 나타났다. 그러나 가족(친척)은 사회적 지지와 사회적 부정성 모두에서 영향력이 유의미하지 않은 것으로 나타났다. 이러한 결과를 바탕으로 농촌 노인의 정신건강 증진을 위한 긍정적 사회적 관계 형성의 중요성 및 실천적 함의를 제시하였다. In order to examine the effects of social relationship on mental health of the rural elderly, this study especially focused on testing the differential effects of social support and social negativity on their depressive symptoms. Data from 「Korean Social Life, Health and Aging Project(KSHAP)」 was used for analyzing 569 elderly aged over 60 who were living with spouses. The results showed that both social support and social negativity had statistically significant impact on rural elderly’s depressive symptoms. Furthermore, social negativity showed more influence on depressive symptoms than social support. Both social support and social negativity from spouse had statistically significant effect on depressive symptoms. However, for friends and neighbors, only social negativity showed statistically significant effect on depressive symptoms. Social support and negativity of family members did not have any significant impact. Implications for paying more attention to the quality of social relationships and for fostering more positive relationships within social network of the rural elderly were suggested.

Fe 첨가된CuO의 구조적, 자기적 특성

박영란(Young Ran Park),김광주(Kwang Joo Kim),박재윤(Jae Yun Park),안근영(Geun Young Ahn),김철성(Chul Sung Kim) 한국자기학회 2006 韓國磁氣學會誌 Vol.16 No.1

Pure and Fe-doped CuO thin-film and powder samples were prepared using a sol-gel method. Undoped CuO films exhibited monoclinic structure and p-type electrical conductivity (~10-² Ω-¹ ㎝-¹) due to copper deficiency. On the other hand, CuO : Fe films were found to be insulating and Li doping on the films led to a restoration of p-type conductivity and a ferromagnetic hysteresis behaviour at room temperature. The observed properties for the CuO : Fe, Li films can be explained in terms of hole creation by substitution of Li+ for Cu²+ sites and mediation of long-range interactions between Fe³+ ions by the Li+-induced defect states. CuO : Fe powders exhibited a ferromagnetism at room temperature with its strength being dependent on post-annealing temperature. Mossbauer measurements on the CuO : Fe films and powders revealed that the octahedral Cu²+ sites are mostly substituted by Fe³+ ions.

영한번역에서의 시제의 등가성과 기계번역과의 비교

박영란(Park Young ran) 한국통번역교육학회 2017 통번역교육연구 Vol.15 No.1

This paper focuses on how tense equivalence is maintained when English present progressives are translated into Korean and on how university students translations are different from those derived from translation machines. The source text is selected from COCA according to Vendler s verb categories. Based on the analyses of 30 students questionnaires, two findings are identified. First, the tense equivalence in English-Korean translation is affected by verbal types. The activity verbs strongly keep the tense equivalence and then achievement and accomplishment verbs follow. The tense equivalence in state verbs is relatively weak. Second, the machine translation keeps the tense equivalence between source and target texts, but students seem to use several tenses: the present, the progressive, the future, and the past. With regard to abstract nouns, adjectives, adverbs, adverb phrases, the machine produces more monotonous translations than students.

CFD 해석을 이용한 Balloon형 인공심폐기 설계를 위한 구조적 해석

박영란 ( Young Ran Park ),심정연 ( Jeong Yeon Shim ),김기범 ( Gi Beum Kim ),김상진 ( Shang Jin Kim ),강형섭 ( Hyung Sub Kang ),김진상 ( Jin Shang Kim ),김민호 ( Min Ho Kim ),홍철운 ( Chul Un Hong ),김성종 ( Seong Jong Kim ) 한국화학공학회 2011 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.49 No.2

In this study, we attempted a structural analysis in order to design a balloon type extracorporeal membrane oxygenator that can induce blood flow without using blood pumps for the purpose of complementing the weakness in the existing extracorporeal membrane oxygenator. To analyze the flow characteristic of the blood flow within the virtual model of extracorporeal membrane oxygenator, computational fluid dynamics(CFD) modeling method was used. The operating principle of this system is to make the surface of the extracorporeal membrane oxygenator keep contracting and dilating regularly by applying pressure load using a balloon, and the ``Time Function Value`` that changes according to the time was applied by calculating a half cycle of sine waveform and a cycle of sine. waveform Under the assumption that the uni-directional blood flow could be induced if the balloon type extracorporeal membrane oxygenator was designed as per the method described above, we conducted a structural analysis accordingly. We measured and analyzed the velocity and pressure of blood flow at both inlet and outlet of the extracorporeal membrane oxygenator through CFD simulation. As a result of the modeling, it was confirmed that there was a flow in accord with the direction of the blood by the contraction/dilation. With CFD simulation, the characteristics of blood flow can be predicted in advance, so it is judged that this will be able to provide the most optimized design in producing an extracorporeal membrane oxygenator.

돌봄 노동 여성의 욕구와 노인요양보장 정책

박영란 ( Yeong Ran Park ) 한국사회보장학회 2007 사회보장연구 Vol.23 No.1

이진영상을 위한 심층암호 기법에 관한 연구

박영란(Young-Ran Park),하순혜(Soon-Hye Ha),강현호(Hyun-Ho Kang),이혜주(Hye-Joo Lee),신상욱(Sang-Uk Shin) 한국멀티미디어학회 2006 멀티미디어학회논문지 Vol.9 No.2

카툰 화상을 이용한 심층암호에 관한 연구

박영란(Young-Ran Park),박지환(Ji-Hwan Park) 한국멀티미디어학회 2004 멀티미디어학회논문지 Vol.7 No.7

오차 확산법을 이용한 기밀 데이터 합성법

박영란(Young-Ran Park),이혜주(Hye-Joo Lee),박지환(Ji-Hwan Park) 한국멀티미디어학회 1999 멀티미디어학회논문지 Vol.2 No.2

산소 결핍된 TiO₂-δ 박막의 철 도핑에 의한 전기적, 자기적 특성 변화

박영란(Young Ran Park),김광주(Kwang Joo Kim),박재윤(Jae Yun Park),안근영(Geun Young Ahn),김철성(Chul Sung Kim) 한국자기학회 2006 韓國磁氣學會誌 Vol.16 No.1

Oxygen-deficient anatase and rutile titanium dioxide (TiO₂-δ) thin films were prepared by a sol-gel method and their structural, electronic, and magnetic properties were investigated. Both anatase and rutile TiO₂-δ : Fe films exhibited ferromagnetism at room temperature for a limited range of Fe doping. For the same amount of Fe doping, the anatase sample exhibited a higher magnetic moment than the rutile one. Result of conversion electron Mossbauer spectroscopy measurements indicates that Fe³+ ions substituting the octahedral Ti⁴+ sites mainly contribute to the room-temperature ferromagnetism. Some of the anatase TiO₂-δ : Fe films exhibited p-type character but the observed ferromagnetism turns out to be independent of the hole concentration. The room-temperature ferromagnetism can be explained in terms of a direct ferromagnetic coupling between two neighboring Fe³+ ions via an electron trapped in oxygen vacancy in TiO₂-δ : Fe.