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토니 모리슨의『빌러비드』에 나타난 탈식민적 정체성 추구
박순정 ( Sun Jung Park ) 한국현대영어영문학회 2012 현대영어영문학 Vol.56 No.4
The purpose of this paper is to examine Beloved by Toni Morrison from the viewpoint of post-colonial framework. In the novel, Morrison seeks a way for ex-slaves to regain a free, autonomous self. In so doing, the blacks have to ask themselves, "What do I want to do?" Then their answers would be to recover the status as a human subject the slavery discourse denied. One of the characters, Paul D, with the Emancipation, reclaiming his body, is still in the state of slave of the past, so he needs to liberate himself from the rememory. So, as a catalyst, Beloved enters the narrative and stimulates him to face his repressed past. Thanks to her, making contact with his own rememory, he redescribes his past favoring present life. As a result of it, finding his own desire of making a home with Sethe, he finally achieves the post-colonial self realization. (Chungnam University).
유격훈련이 신체조성과 혈중 지질치 및 운동 능력에 미치는 영향
이정환 ( Jung Hwan Lee ),남민정 ( Min Jung Nam ),이건희 ( Geon Hui Lee ),최웅림 ( Woong Rim Choi ),이민주 ( Min Joo Lee ),박순정 ( Sun Jung Park ),양동희 ( Dong Hee Yang ) 국군의무사령부 2014 대한군진의학학술지 Vol.45 No.1
Objectives: Soldiers are expected to maintain the highest possible level of physical strength and health for military operation. The ranger training augments individual physical readiness of each solider for combat situation. However, there are fewer researches for ranger training. Hence, we research and analyze the changes of body composition, physical fitness and lipid profile among a group of soldiers during a week of ranger training. Methods: 68 of young men were enrolled in their ranger training program and one man was excluded. Before and after the training, Body composition was measured by bioelectrical impedance assessments (BIA). Lipid profile and physical fitness was also assessed. Results: After one week, 51 men completed the training and 16 men did not complete. Fat mass, fat percentage and visceral fat gained among the completed training group. LDL cholesterol of both group decreased. After the training, increased grip and leg power and agility of both group were resulted. However balance and flexibility increased only in completed training group Conclusions: one week ranger training could reinforce some of physical performance but could not be enough to improve body composition and lipid profile of soldiers. Hence, at the sight of individual health, we need to consider reforming ranger training.
유전자 전달체로 응용하기위해 Folate와 Poly-L-lysine이 결합된 O-carboxymethyl Chitosan의 제조
정경원 ( Gyeong Won Jung ),박순정 ( Sun Jung Park ),김영민 ( Young Min Kim ),남정표 ( Joung Pyo Nam ),최창용 ( Changyong Choi ),박성철 ( Seong Cheol Park ),나재운 ( Jae Woon Nah ),장미경 ( Mi Kyeong Jang ) 한국키틴키토산학회 2013 한국키틴키토산학회지 Vol.18 No.4
In present study, PLL-OCMCh-PEG-FA (POCPF) was prepared to apply the cell targeting gene delivery carrier. The POCPF copolymer was characterized by 1-proton nuclear magnetic resonance ( 1 H-NMR). The result indicated that POCPF has been successfully synthesized. The particle size of the POCPF was 188 ~ 320nm, which was measured by DLS (Dynamic Light Scattering). In addition, gel retardation assay showed that the POCPF has potential to bind with pDNA. Also, it showed that POCPF can be protected pDNA from DNase. The gene transfection efficiency of the POCPF was evaluated in HCT119 cells. The result showed that transfection efficiency of the POCPF was better than the OCMCh and PLL (Poly-L-lysine) via receptor mediate endocytosis between FA and FA receptor on cancer cell surface. The results demonstrated that POCPF copolymer is good candidate as a gene carrier for gene therapy.
인간배아줄기세포 유래 배상체내의 신경세포군 확인과 분화과정 동안 이뤄지는 신경발생과정의 이해
김동환 ( Dong Hwan Kim ),문성환 ( Sung Hwan Moon ),김주미 ( Ju Mi Kim ),이민지 ( Min Ji Lee ),신정민 ( Jeong Min Shin ),이경일 ( Kyung Il Lee ),박순정 ( Soon Jung Park ),정선화 ( Sun Hwa Chung ),김희정 ( Hee Jung Kim ),정형민 ( Hy 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.4
Recently, human embryonic stem cells(hESCs) have been highlighted to understand early embryogenesis as well as to apply to clinical cell therapy. During human embryogenesis, dynamic processes of early neuronal development are very important to elucidate their developing mechanism. However, the mechanism has not been entirely clear. The formation of embryoid bodies(EBs) derived from hESCs is a tissue-like spheroids in suspension culture and has a potential to differentiate into the three germ layers. Even though many kinds of specific cell makers have been studied, the specific proteins expressed from differentiated cells have been elusive. The objective of this study is to investigate the expression of neuronal protein while EBs is differentiating from hESCs(day 5, 10, 15 and 20). The expressions of neuronal specific makers such as Neurofilament 68kDa(NF 68), tubulin III(TuJ-1) and microtubule-associated protein 2(MAP2) in each EBs stage were analyzed by immunofluorescence(IF). The expressions and localization of neuronal specific markers were shown to be different depending on EBs stages. NF 68 positive cells were expressed on the surface of EBs at day 10 and the expression of NF68 almost disappeared at day 20. MAP2 was not detected in EBs at day 10, whereas it began to be slightly detected on the surface of EBs at day 15. On the other hand, TuJ-1 positive cells were found at all stages and their expression levels were maintained until day 20. These results demonstrate neuronal specific markers can be expressed differently depending on EBs stages, suggesting this allows us to probably understand the early development of neurogenesis in hESCs. Furthermore, this idea expands that we are able to understand the development of all specific cells differentiated from early human embryogenesis.
hESC로부터 유래된 혈관내피전구세포를 위한 최적의 배양조건 확립
김주미 ( Ju Mi Kim ),문성환 ( Sung Hwan Moon ),이민지 ( Min Ji Lee ),오인록 ( In Rok Oh ),신정민 ( Jeong Min Shin ),박순정 ( Soon Jung Park ),정선화 ( Sun Hwa Chung ),김문규 ( Mun Kyou Kim ),이경일 ( Kyung Il Lee ),정형민 ( Hyung 한국조직공학과 재생의학회 2008 조직공학과 재생의학 Vol.5 No.3
Human embryonic stem cell(hESC) can be very valuable source for the cell therapy due to their pluripotency when they differentiated into specialized cell types. Conventionally, differentiated cell population were obtained from spontaneously formed embryoid body(EB) which could contain various cell types. In this study, we described the optimization of culture condition for endothelial precursor cells derived from human embryonic stem cells(hESC-EPCs). First, we compared the expression of endothelial precursor cell markers such as CD133, CD34 and KDR in day 7 hEBs and isolated CD133 and KDR double positive population as hESC-EPCs. Second, we compared the culture medium and ECM coating to optimize culture condition for hESC-EPCs. As the result, we could confirm EGM-2 and collagen coating were optimized condition to culture of hESC-EPCs. After conducting optimized culture condition for hESC-EPCs, we examined the maintenance of characteristics of hESC-EPCs cultured in optimized culture condition as vascular cells. Finally, we could confirm the characteristics of hESC-EPCs cultured in optimized condition as vascular cells were maintained in developed optimized culture condition. Furthermore, the optimized culture condition developed in this study will be useful to culture of other vascular lineage cells derived from hESCs.