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The Effects of Cninnire(L)Cuss on the Esscnrial Elements to the Activated Macrophages
박세봉,고우신,Park, Se-bong,Ko, Woo-Shin The Society of Korean Medicine Ophthalmology 2000 한방안이비인후피부과학회지 Vol.13 No.1
外治에 응용되는 蛇床子의 抗炎症效果를 살펴보고자 NO의 生成濃度, 細胞 生存度, iNOS의 합성 및 $TNF-{\alpha}$의 生成濃度를 實驗하였다. 그 結果 NO의 濃度는 顯著하게 減少하였고, 細胞 生存度는 蛇床子에 의하여 影響을 받지 않았으며, iNOS의 합성에 있어서 iNOS의 濃度가 顯著하게 낮아졌고, $TNF-{\alpha}$의 濃度는 顯著하게 減少하였다. 以上을 살펴볼 때 蛇床子가 iNOS 및 $TNF-{\alpha}$의 생성자체의 억제를 통하여 NO의 合性을 조절함으로써 蛇床子가 충분한 抗炎症效果가 있음을 알 수 있었다. 향후 蛇床子의 성분분석등 다양한 연구가 진행되어야 할 것으로 생각되며, 이를 통하여 앞으로 抗炎症效果가 우수한 약제의 개발이 가능할 것으로 思料된다.
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박세봉,고우신,Park, Se-bong,Ko, Woo-shin 대한한방안이비인후피부과학회 1999 한방안이비인후피부과학회지 Vol.12 No.1
Based on the research of cataract, glaucoma, bluish glaucoma, we have abtained the following results. 1. Main etiologies of the cataract are deficiency of the liver and kidneys, main symptoms are no nebula, no pain, no tear and sometimes light blue and white vision appear. 2. Main etiologies of glaucoma are receives heat from the liver, stagnation of the liver and gall bladder etc, main symptoms are both forehead corner pull each ether, pain from eye ball to nasal septum, headache, extending pain of the eyes, sometimes red and white flower vision appear, etc. 3. main etiologies of the bluish glaucoma are melancholy, wrath, stagnation of the liver, internal damage, flaming up fire of the phlegm, deficiency of the liver, deficiency of blood, etc, main symptoms are numbness, visual field constriction, blindness, reduce of visual capacity, blue flower vision sometimes appear, etc. 4. Treatments are mainly uses nourish Um(陰) to reduce pathogenic fire, relieve stagnancy of Ki(氣) of the liver, nourish Um(陰) of the liver and kidneys, etc, acupuncture points are mostly contended arounded eyes such as BL10)(天柱), GV16(風府), LR3)(太衝), HT5)(通里) and in the case of nauses and vomitting PC6)(內關, ST36)(足三里) are included in addition to the above.
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박세봉,김종한,고우신,Park, Se-Bong,Kim, Jong-Han,Ko, Woo-shin 대한한방안이비인후피부과학회 1998 한방안이비인후피부과학회지 Vol.11 No.1
A Literatur Review of Physical and Breathing Exercises for Ear Health, the results were as follows. 1. The most common using way is rubbing your ears and auricle with warm palms. 2. To stimulate inner acoustic meatus, obstruct external acoustic meatus for a short time by the palm or fingers and then to stimulate it by the sudden opening. 3. This method is a common way to press aurical or orifice of external acoustic meatus for example, TE21(耳門), TE20(角孫), GB2(聽會), TE19(노息), TE18(계脈), GB11(竅陰), TE17(예風) etc. 4. The best time to pratice is early in the morning or before go to bed, but a litter difference by the writer's opinion, so there is not consistency. 5. Since tinnitus and hardness of hearing is related to the who1e body, it is known that you can relax your whole body by pressing the acupuncture points located in the head of occipital region or by stretching the upper half of the body. 6. There is method that obstruct your ears with palms and 3rd finger on your occipital region and splash by the 2th fingers.(sound the celestial drum)
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유근피 추출물이 RBL-2H3 비만세포에서 ${\beta}$-hexosaminidase 및 cytokine 분비에 미치는 효과
박세봉,강경화,윤화정,고우신,Park, Se-Bong,Kang, Kyung-Hwa,Yoon, Hwa-Jung,Ko, Woo-Shin 대한한방안이비인후피부과학회 2011 한방안이비인후피부과학회지 Vol.24 No.1
Objectives : Ulmus davidiana (UD) has been widely used in Korean herbal medicines used for treatment of acute and chronic inflammatory diseases, such as rhinitis, asthma, and abscess. In this study, To investigated the protective effect of UD on type 1 allergic response, we determined whether UD inhibits early and late allergic response. Methods : The effect of UD was analyzed by ELISA and RT-PCR in RBL-2H3 cells. Levels of ${\beta}$ -hexosaminidase, interleukin (IL)-4 and TNF-${\alpha}$ were measured using enzyme-linked immunosorbent assays (ELISAs). mRNA levels of COX-2 and T-helper type 2(Th2) cytokines were analyzed with RT-PCR. Results : We found that UD suppressed ${\beta}$-hexosaminidase release in RBL-2H3 not only by the PMA plus A23187 stimulation, but also by the IgE-DNP-HSA stimulation at the antigen-antibody binding stage and antibody-receptor binding stage. UD also significantly inhibited COX2 level, along with reduced Th2 cytokine levels, such as IL-3, IL-4, IL-5, IL-13, GM-CSF, and TNF-${\alpha}$ in RBL-2H3. Conclusions : Our results indicate that UD protects against type 1 allergic response and exerts an anti-inflammatory effect through the inhibition of degranulation and expression of COX2 and Th2 cytokines.
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