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흰쥐 대뇌세포의 저산소증 모델에서 석창포(石菖浦 Acori graminei rhizoma. AGR)에 의한 유전자 표현 변화의 microarray 분석
박동준,정승현,문일수,이원철,신길조,Park, Dong-Jun,Jung, Seung-Hyun,Moon, Il-Soo,Lee, Won-Chol,Shin, Gil-Jo 한국생명과학회 2007 생명과학회지 Vol.17 No.1
Acori graminei Rhizomn (AGR) is a perennial herb which has been used clinically as a traditional oriental medicine against stroke, Alzheimer's disease, and vascular dementia. We investigated the effect of AGR on the modulation of gene expression profile in a hypoxic model of cultured rat cortical cells. Rat cerebrocortical cells were grown in Neurobasal medium. On DIV12, cells were treated with AGR $(10ug/m\ell)$, given a hypoxic shock (2% $O_2$, 3 hr) on DIV14, and total RNAs were prepared one day after shock. Microarray analyses indicated that the expression levels of most genes were altered within the global M values +0.5 and -0.5, i.e., 40% increase or decrease. There were 750 genes which were upregulated by < global M +0,2, while 700 genes were downregulated by > global M -0.2. The overall profile of gene expression suggests that AGR suppresses apoptosis (upregulation of anti-apopotic genes such as TEGT, TIEG, Dad, p53, and downregulation of pro-apopotic genes such as DAPK, caspase 2, pdcd8), ROS (upregulation of RARa, AhR), and that AGR has neurotrophic effects (upregulation of Aktl, Akt2). These results provide a platform for investigation of the molecular mechanism of the effect of AGR in neuroprotection.
흰쥐의 제 2 형 Collagen 유발 관절염 : IgG 항 Collagen 항체의 변화
박동준(Dong Jun Park),조철수(Chul Soo Cho),김호연(Ho Youn Kim),김동집(Dong Jip Kim) 대한내과학회 1991 대한내과학회지 Vol.41 No.3
N/A Collagen-induced arrthritis (CIA) is a chronic inflammatory arthritis in rodents immunized with type II collagen, a major protein of hyaline cartilage. Because CIA shares clinical, histologic, and immunologic features with human rheumatoid arthritis, it has been regarded as an important animal model for elucidation of the pathogenesis and therapeutic approach to this disease. To investigate the role of humoral immunity to type II collagen in the initiation of CIA, the authors evaluate the clinical features and sequential changes of IgG anticollagen antibody(ACA) in CIA(n=6), IgG ACA to native bocine type II collagen was measured by enzyme-linked immunosorbent assay. The results were as follows: 1) The incidence of CIA was 67% (6 out of 9 rats), and the mean number of involved limbs per rat in the arthritic group was 2.2±0.4. The mean day of arthritic onset was the 22th day, ranging from the 12th to the 25th day after immunization. 2) IgG ACA to native bovine type II collagen in the arthritic group (n=6) was significantly higher than that in the control group (n=9) from the 2nd to the 8th week after initial immunization (p<0.003). In the arthritic group IgG ACA reached a peak level in the 4th week and then slightly decreased. However, it showed a persistently higher level until the 8th week after immunization, Th results suggest that humoral immunity to type II collagen has an important role in the initiation of CIA.